• Microbiology and Biotechnology Letters
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• v.25 no.6
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• pp.623-629
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• 1997

The studies of isolation and physiological characteristics of mesophilic or thermophilic bacteria from thermophilic oxic process (TOP) treating Chinese restaurant wastes were conducted. Chinese restaurant wastes were consist of moisture; 75.8%, solids; 24.2% and ash; 0.49%. The volatile solid was about 99% of total dry solids. In wastes used in this experiment, there was content of crude protein; 4.47%, crude lipid; 3.56%, free sugar, 0.4% , crude starch; 10.34% and crude fiber 3.14%, respectively. And then it has about 4,970 kcal/kg-dry solid of Chinese restaurant wastes. From TOP treating the chinese restaurant wastes, 37 strains of mesophilic or thermophilic bacteria were primarily isolated using medium used for the isolation and among them 6 strains of thermophilic and 7 strains of mesophilic bacteria were selected by testing the activities of amylase, cellulase, protease and lipase. TB-1, TB-9 as thermophilic bacteria and MB-15-1, 15-2, MB23 as mesophilic bacteria having strong enzyme activity were selected among isolated strains. All selected strains reduced nitrate to nitrite and they utilized glucose, manose, manitol, and maltose as carbon source. From these MB15-2 was identified as Bacillus cereus, TB1, Bacillus licheniformis and TB9; Bacillus schlegelii.

• Journal of Korean Society of Water and Wastewater
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• v.21 no.4
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• pp.467-475
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• 2007

This study was conducted to examine temperature effects on hydrogen production in anaerobic fermentation. 18 batch reactors were operated at mesophilic ($35^{\circ}C$) and thermophilic conditions ($55^{\circ}C$) to achieve maximum hydrogen production in anaerobic fermentation. Optimum hydrogen production conditions were also investigated at each temperature. Different trends were observed regarding pH effects on hydrogen production. This effect was not significant for mesophilic fermentation ($35^{\circ}C$). In this case, pH may not drop to interfere hydrogen production during the test. However, hydrogen production decreased without pH control for thermophilic condition ($55^{\circ}C$). Effects of heat treatment were observed for both fermentation process. Hydrogen production with heat treatment was higher than hydrogen production without heat treatment for both fermentation processes. The amount of produced hydrogen for each substrate concentration with temperature changes showed that more hydrogen was produced at $35^{\circ}C$ than at $55^{\circ}C$.

• Journal of Microbiology and Biotechnology
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• v.2 no.1
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• pp.50-55
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• 1992

A genomic library of a mesophilic cellulolytic anaerobe, Clostridium sp. KCTC 8440 DNA was constructed in Escherichia coli using plasmid pUC9. Clones of E. coli exhibiting carboxymethyl cellulose-hydrolyzing activity (CMCase) were isolated and divided into seven types based on the restriction enzyme patterns of recombinant plasmids. E. coli strains carrying type A genes showed activity on carboxymethyl cellulose about 7-8 times greater than clones carrying genes of other types. Restriction maps of the cloned DNA fragments were determined, and homologies between them were investigated. The results suggest that Clostridium sp. KCTC 8440 has seven distinct CMCase genes.

• Journal of Environmental Health Sciences
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• v.26 no.4
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• pp.15-20
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• 2000

The studies of swine wastewater treatment aim to development of process using soil microorganism. Removal rate of swine wastewater containing organic matter was 99 percent in case of high loading rate. Microorganism was devoted to improve the treatment efficiency of the process. According to the result obtained from biological treatment of high loading rate swine wastewater. Hydraulic retention time was 2.3 days in unit process of biological phosphorus removal. BO $D_{rm}$ / $P_{rm}$ ratio was 1122 in room temperature anaerobic process and 355.6 in mesophilic anaerobic process. And then phosphorus removal rate mesophilic anaerobic process was 3 time as much as than room temperature acaerobic process.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.291-294
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• 1997

The purpose of this study was to investigate the change of microbial flora of using coal fly ash as humidity conditioner during the household garbage composting.The summarized results of microbial flora were as follows:There was no difference of t he seasonal fluctuation of mesophilic and themophilic microorganisms.The population of thermophilic actinomycetes was rapidly increased in winter,but not much changed in spring and summer.Thermophilic and mesophilic fungal flora were increased at the same time,but the population of thermophilic fungal flora was smaller than that of mesophilic. The population of bacteria, actinomycestes and fungi showed not much difference.

• Journal of Microbiology
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• v.39 no.3
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• pp.232-235
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• 2001

Electrophoretic resolution of superoxide dismutase (SOD) from the highly UV-resistant bacteria, Deinococcus species revealed multiple forms of superoxide dismutases (SODs) in D. radiodurans, D. grandis, and D. proteolyticus, as judged from electrophoretic properties and metal cofactors. A single SOD occurred in both D. radiophilus and D. radiopugnans. Deinococcal SODs were either MnSOD, FeSOD or cambialistic Mn/FeSOD. The unique SOD profile of each mesophilic Deinococcus species, multiplicity and metal cofactors would be valuable in identifying Deinococcus species.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.1
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• pp.585-592
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• 2014

Mixing effect on anaerobic digestion of livestock wastewater was different results depending on the researchers have been reported. The purpose of this study was to understand application of Korea livestock waste it was necessary to determine the effect of mixing. 4 anaerobic reactors were operated mesophilic and thermophilic temperature with continuous mixing or non mixing condition, respectively. Experimental result showed If temperature was same, TCOD removal efficiency of continuous mixing reactor was 0.11-0.58% higher than non mixing reactor. Different mesophilic and thermophilic temperature, there was no significant difference of TCOD removal efficiency. Continuously mixed digester gas production was 1.7-4.6% higher than non mixed digester. In addition, mesophilic digester gas production was 29.1-32.1% higher than the thermophilic digester. It was due to the thermophilic digester believe the inhibition of ammonia. This study suggest that the optimized operation condition of anaerobic digestion for livestock wastewater treatment was mesophilic continuous mixing state.

• Korean Journal of Environmental Agriculture
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• v.16 no.1
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• pp.44-47
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• 1997

The change in microbial flora has been studied through dwelling house composting by the composter with double layer walls. The results are summarized as followes. 1. Mesophilic bacteria increased and decreased mildly, thermophilic bacteria showed a tendency to decrease except for spring, and the number of mesophilic bacteria and thermophilic bacteria had a tendency to increase and decrease simultaneously. 2. The number of mesophilic actinomycetes were increased at the early stage of compositing in winter, mildly decreased in spring and slightly decreased in summer, and the number of thermophilic actinomycetes were decreased at the early stage of composting. 3. The decrease in the number of mesophilic fungi was observed at the middle stage in summer, but the mild increase was observed in spring and winter. The number of thermophilic fungi was generally decreased. 4. Ammonia oxidizer and nitrite oxidizer were observed in this field composting much more than in the other composting experiments.

• Applied Chemistry for Engineering
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• v.23 no.1
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• pp.28-34
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• 2012

In this study, we operated a UASB (upflow anaerobic sludge blanket) reactor by using foodwaste leachate as a raw material with the method of Mesophilic Digestion ($35{\pm}0.5^{\circ}C$) and Thermophilic Digestion ($55{\pm}0.5^{\circ}C$). During 20 days of operating time with the Mesophilic Digestion, the recirculation ratio of effluent was stepwisely changed in every five days. Thermophilic Digestion was carried out at the same condition for Mesophilic Digestion. Results showed that the organic removal efficiency of Mesophilic Digestion was over 90% and the yield of methane production was from 66 up to 70%. The organic removal efficiency of Thermophilic Digestion was over 80% and the yield of methane production was between 62 to 68%. Also, when UASB reactor was operating to over the 3Q effluent recirculation, the experiment could be carried out economically and stably.

• Korean Chemical Engineering Research
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• v.61 no.2
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• pp.278-286
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• 2023

The present work evaluated the production of biohydrogen under mesophilic and thermophilic conditions through dark fermentation of palm oil mill effluent (POME) in batch mode using the design of experiment methodology. Response surface methodology (RSM) was applied to investigate the influence of the two significant parameters, POME concentration as substrate (5, 12.5, and 20 g/l), and volumetric substrate to inoculum ratio (1:1, 1:1.5, and 1:2, v/v.%), with inoculum concentration of 14.3 g VSS/l. All the experiments were analyzed at 37 ℃ and 55 ℃ at an incubation time of 24 h. The highest chemical oxygen demand (COD) removal, hydrogen content (H₂%), and hydrogen yield (HY) at a substrate concentration of 12.5 g COD/l and S:I ratio of 1:1.5 in mesophilic and thermophilic conditions were obtained (27.3, 24.2%), (57.92, 66.24%), and (6.43, 12.27 ml H₂/g COD_rem), respectively. The results show that thermophilic temperature in terms of COD removal was more effective for higher COD concentrations than for lower concentrations. Optimum parameters projected by RSM with S:I ratio of 1:1.6 and POME concentration of 14.3 g COD/l showed higher results in both temperatures. It is recognized how RSM and optimization processes can predict and affect the process performance under different operational conditions.