Odontoclasts and osteoclasts resorb tooth root and alveolar bone, respectively. Many studies have focused on osteoclast formation in periodontitis, but effect of periodontitis on odontoclast formation is not clearly clarified. In this study, we observed formation of odontoclasts as well as osteoclasts in rats with ligature-induced periodontitis. To induce periodontitis, ligatures were placed around the first molars in left mandibles of rats. Rats were sacrificed at days 1, 3, and 10 after ligation. After tartrate resistant acid phosphatase (TRAP) staining in mandible section, the number of TRAP-positive odontoclasts and osteoclasts were histologically counted along the root and the alveolar bone surfaces of tooth, respectively. Odontoclasts increased until day 10 in mesial and furcation root surface, but did not increase in distal root surface. When compared odontoclast formation to osteoclast formation in mesial surface, osteoclasts peaked at day 3, and then decreased gradually, whereas odontoclasts were continuously increased until day 10. The number of odontoclasts was lower than that of osteoclasts before and after periodontitis induction. These indicate that periodontitis increased formation of odontoclasts as well as osteoclasts, but odontoclast formation occurs slower and weaker than that of osteoclasts.
Kim, Eui-Seong;Kim, Hyun-Jung;Seo, Deog-Gyu;Roh, Byoung-Duck
Restorative Dentistry and Endodontics
/
v.34
no.3
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pp.232-239
/
2009
The aim of this study was to compare the effects of anti curvature filing with stainless steel k-file versus nickel-titanium ProFile in the shaping of mesial root canals of extracted mandibular molars. A total of 60 canals from 30 mesial roots of mandibular molar teeth were randomly assigned to three groups with n=20 each. They were prepared with different instruments and methods: The first group with stainless steel k-file and circumferential filing. the second with precurved stainless steel k-file and anticurvature filing and the third with ProFile C06 taper) and anticurvature filing. Using a micro-computed tomography system (skyscan-1076, SKYSCAN, Antwerpen, Belgium), pre-and post-operative specimens were scanned. Subsequently, canal images were superimposed and changes in root dentin thickness were measured at distal side (danger zone) of the canal. The data was analyzed using a one-way ANOVA and the comparison of means was conducted using a post hoc multiple comparison Tukey test. There were significant differences in the change of root dentin thickness at the $7.5{\sim}8.5\;mm$ level between group 1 and 2, $3.5{\sim}6mm$ level between group 1 and 3 and $3.5{\sim}6mm$ level between group 2 and 3(n=20, P<0.05).
The purpose of this study was to investigate the displacement of and the stress distribution on the prosthesis, abutment, and its supporting tissues under functional load, and the effect of alteration in root length of 2nd abutment. The 3-dimensional finite element method was used and the finite element models were prepared in which the abutments of left mandibular 5 unit axed partial denture were canine, the 1st pre-molar and the 2nd molar, and the root lengths of canines were as follows. Model I : Root length of canine was 2mm longer than the 1st premolar Model II : Root length of canine was 2mm shorter than the 1st premolar Static compressive force of 300N was applied to connector between 2nd premolar & 1st molar, and then von Mises stress, displacement and reaction force were obtained. The results were as follows : 1. In fixed partial denture, prosthesis under load on pontic was rotated around mesio-distal long axis of it from longual side to buccal, and simultaneously bended in buccal and gingival direction with mesial end deformed in gingival direction and distolingual end in occlusal. 2. Clinical crowns of abutments were bended in the same directions with those in which prosthesis deforms. Due to that, roots of anterior abutments were twisted in counterclockwise with concentration of shear stress on distal or distobuccal sides of their cervices, and that of posterior was in clockwise with concentration of shear stress on mesiobuccal side of it in the same level with anterior abutments. 3. In case that root length of the 2nd abutment was longer than that of the 1st abutment, its displacement and reaction force which means the force tooth exerts on the surrounding periodontal tissues were smaller but shear stress on itself was larger than in the case root length of 2nd abutment was shorter.
In order to evaluate the therapeutic effect of argon laser irradiation on hypersensitive root, 60 mandibular premolars that had been extracted due to periodontal disease were treated by thorough scaling, root planing and root conditioning with tetracycline HCI(100mg/ml, 5 min.) for induction of hypersensitive root. Within middle one third of root mesial surface(30) or distal surface(30) was randomly irradiated by 0.5W-, 0.75W- and 1.0W-power for 2 minutes argon laser(HGM Inc., USA) with or without topical application of 1.23% acidulated phosphate fluoride gel(Pascal, USA). After all teeth were maintained in 2% methylene blue solution for 48 hours and cross-sectioned through irradiated surface of dried roots, methylene blue stained area was compared between irradiated side and nonirradiated side. Following results were obtained : 1. Methylene blue stained area was significantly less in the laser irradiated group than control group(p<0.05). 2. There was no significant difference in methylene blue stained area between 0.5W-power and 0.75W-power group(p>0.1) and between 0.75W-power and 1.0W-power group(p>0.1), but there was significant difference between 0.5W-power and 1.0W-power group(p<0.05). 3. There was no significant difference in methylene blue stained area between two groups with and without topical application of 0.12% acidulated phosphate fluoride gel(p>0.1). The results suggest that argon laser irradiation on hypersensitive root may reduce the hypersensitivity by obstruction of dentinal tubules.
The purpose of this study was to analysis the stress distribution induced by three unit PFM bridges and various cantilever bridges replacing maxillary latersal incisor. The simplified two-dimensional photoelastic models used for this study was contructed in the folio- wing way. CR/R ratio was designed to be 1 : 1, 1 : 1.25 and 1 : 1.5. The pontics of cantilever bridge supported by maxillary canines consisted of wrap-around type, rest-extension type, and simple type. 3-unit PFM bridge was constructed with traditional method. 1kg vertical static load was applied on the center of the incisal edge of the pontic. The stress pattern was examined and recorded by photography. The results obtained were as follows ; 1. The magnitude of stress on the abutment root apex area of a traditional 3-unit bridge was the lowest. 2. The model of cantilevered pontic with a rest showed the relatively well distributed stress around the abutment tooth. The model with simple pontic generated the greatest stress concentration in the supporting structure of the abutment tooth. 3. As the height of bone level reduced, the rotational and vertical force increased around the abutment tooth. 4. The stress concentration of the 3-unit bridges occured on the root apex and stress concentration of the cantilever briage occured on the root apex and cervix area, 5. In the case of the cantilever bridge, stress concentrated distally on the root apex area of the abutment tooth and additional stress was observed mesially on the upper part of the root. Especially in the case of the simple pontic, was phenomenon was more apparent than the others. 6. Force applied to cantilevered pontic was transmitted to the adjacent central incisor through the contact surface. Stress was markedly observed on the mesial cervix area in the case of simple pontic and on the root apex area in the case of wrap-around type and rest-extension type.
Objective: To investigate changes in the immature teeth of Sprague-Dawley rats during orthodontic treatment and to explore the changes in the peri-radicular alveolar bone through micro-computed tomography (CT). Methods: Twenty-five 26-day-old male Sprague-Dawley rats were included. The maxillary left first molar was moved mesially under a continuous force of 30 cN, and the right first molar served as the control. After orthodontic treatment for 7, 14, 21, 28, and 42 days, the root length, tooth volume, and alveolar bone mineral density (BMD) around the mesial root were measured through micro-CT. Results: The immature teeth continued to elongate after application of orthodontic force. The root length on the force side was significantly smaller than that on the control side, whereas the differences in the volume change between both sides were not statistically significant. Alveolar bone in the coronal part of the compression and tension sides showed no difference in BMD between the experimental and control groups. The BMD of the experimental group decreased from day 14 to day 42 in the apical part of the compression side and increased from day 7 to day 42 in the apical part of the tension side. The BMD of the experimental group decreased in the root apex part on day 7. Conclusions: The root length and volume of immature teeth showed continued development under orthodontic forces. Alveolar bone resorption was observed on the compression side, and bone formation was observed on the tension side.
Journal of the korean academy of Pediatric Dentistry
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v.36
no.4
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pp.556-562
/
2009
Ectopic eruption of the maxillary first permanent molar means that the molar erupts out of the normal position and is arrested in its eruption by the second primary molar. This local eruption disturbance results in a premature atypical resorption on the distal part of the second primary molar. In most irreversible cases, the second primary molar is lost prematurely, either by spontaneous exfoliation or by extraction, In cases of doubt as to whether the eruption is of the irreversible type or not, careful radiographic observation period for a few months would be valuable in evaluating the possibilities of the tooth's freeing itself. The purpose of this study was to determine the characteristics and occurrence of the ectopic eruption of the maxillary first permanent molar. A descriptive, observational, retrospective study was done using the radiographs of 25 conseutive patients, who were in the first phase of mixed dentition. A method was designed to evaluate the amount of pathologic resorption of the second maxillary primary molar and the mesial angulation of the first permanent molar. The study showed that the most important etiologic factor was the eruption path or mesial angulation of the first permanent molars relative the chosen reference lines.
Objective: To evaluate the short-term effect of fangchinoline, an anti-inflammatory drug widely used in Asia, on root resorption that is associated with orthodontic tooth movement. Methods: Twenty-four Wistar rats were randomly divided into 6 groups. Mesial forces of 0, 50, or 100 g were applied to the maxillary first molar of the rats in each group for 14 days by activating nickel-titanium closed-coil springs. One-half of the rats receiving each of these treatments also received injections of 200 ${\mu}L$ fangchinoline every 2 days. Finally, movement of the maxillary first molars was measured using digitized radiographs. The molars were extracted and the surfaces of the root resorption craters were recorded using a scanning electron microscope. The distance the molars moved and resorption-area ratio was measured, and results were analyzed using 2-way ANOVA tests. Results: There were no statistical differences in the distances the first molars moved under 50 or 100 g force, regardless of treatment with fangchinoline. However, the resorption area ratios were significantly smaller in those rats that were treated with both tension and fangchinoline than in those rats treated by tension alone. Conclusions: Fangchinoline reduced the resorption area ratio in rats and is therefore an important means of alleviating root resorption.
Purpose: This study evaluated anatomical variations in the root canals of the lower premolars and molars in a Brazilian sub-population using cone-beam computed tomography (CBCT). Materials and Methods: In total, 121 CBCT images of patients were selected from a database. All images contained lower first and second premolars and molars on both sides of the arch, fully developed roots, and no treatment, resorption, or calcifications. In each image, the root canals of the lower premolars and molars were evaluated according to the Vertucci classification in On-Demand 3D software in the multiplanar reconstruction with dynamic navigation. Twenty-five percent of the images were re-assessed to analyze intraobserver confidence with the kappa test. Data were statistically evaluated with linear regression to evaluate the correlations of anatomic variations with age and sex, and the Wilcoxon test to analyze the laterality of variations, with a significance level of 5%. Results: The intraobserver agreement (0.94) was excellent. In general, the root canals of lower premolars and molars showed a higher prevalence of type I than other Vertucci classification types, followed by type V in premolars and type II in molars. When the molar roots were evaluated separately, type II was more frequent in mesial roots and type I in distal roots. Although age showed no correlations with the results, sex and laterality showed correlations with tooth 45 and the lower second premolars, respectively. Conclusion: The lower premolars and molars of a Brazilian sub-population showed a wide range of root canal anatomic variations.
Hadi Rajeh Alfahadi;Saad Al-Nazhan; Fawaz Hamad Alkazman;Nassr Al-Maflehi; Nada Al-Nazhan
Restorative Dentistry and Endodontics
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v.47
no.2
/
pp.24.1-24.15
/
2022
Objectives: Regenerative endodontic treatment is a clinical procedure aimed at biologically regenerating damaged root canal tissue of immature permanent teeth. This study aimed to report the outcomes of regenerative endodontic treatment performed by endodontic postgraduate students. Materials and Methods: Clinical and radiographic data of 27 patients, aged 10-22 years, who underwent regenerative treatment of immature permanent teeth from 2015 to 2019 were followed up, wherein clinical and radiographic examinations were performed for each patient. Postoperative success rate and tooth survival were analyzed, and the postoperative radiographic root area changes were quantified. Results: A total of 23 patients attended the dental appointments, showing that all teeth survived and were asymptomatic. Specifically, 7 periapical pathosis cases were completely healed, 12 were incompletely healed, and 4 cases failed. Moreover, significant differences were found between discolored and non-discolored teeth, and between the presence or absence of periapical radiolucency. Additionally, 3 anterior teeth showed complete closure of the apical foramen, while the apical foramen width was reduced in 17 teeth and failed in 3 teeth. Root length was also found to have been increased in 7 anterior and 4 posterior teeth, and the average length ranged from 4.00-0.63 mm in the anterior teeth, 2.85-1.48 mm of the mesial root, and 2.73-2.16 mm of the molar teeth distal root. Furthermore, calcified tissue deposition was observed in 7 teeth. Conclusions: A favorable outcome of regenerative endodontic treatment of immature permanent teeth with necrotic pulp was achieved with a high survival rate.
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