• Journal of Pharmaceutical Investigation
• /
• 제25권3호
• /
• pp.249-264
• /
• 1995

Although liposome has many advantages as a pharmaceutical dosage form, its application in the industrial field has been limited because of some problems such as preparation method, reproducibility, scale-up, stability and sterilization etc. Liposomes prepared by microemulsification method had defined size, narrow size distribution, reproducibility and high entrapment efficiency. For enhancing the stability, the dry form of liposome was recommended. These types of liposome are proliposome and freeze-dried liposome. The liposome must have some properties for preparing of freeze-dried liposome; small size $(50{\sim}200\;nm)$, narrow size distribution and cryoprotectant. In this experiment, the liposomes containing 5-Fluorouracil(5-FU) and its prodrug(pentyl-5-FU-1-acetate; PFA, hexyl-5-FU-1-acetate; HFA) were made with soybean phosphatidylcholine, cholesterol, stearylamine(SA) and dicetyl phosphate(DCP) employing hydration method or microemulsification method using $Microfluidizer^{TM}$. Both or liposome types were MLV and MEL. After preparation, freeze drying and rehydration were performed. In the process of freezing, trehalose(Tr) was added as a cryoprotectant. Their evaluation methods were as follows; entrapment efficiency, mean particle size and size distribution, dissolution test, retain of entrapment efficiency and turbidity after freeze-drying. The results are summarized as belows. The entrapment efficiency of 5-FU was dependent on total lipid concentration and cholesterol content but that of PFA and HFA was decreased when cholesterol was added. When DCP and SA were added, entrapment efficiency was decreased. As the partition coefficient of drug was increased, entrapment efficiency was increased. Under the same condition, entrapment efficiency of MEL is similar to that of MLV. The mean particle size and size distribution of MEL were smaller than those of MLV. Dissolution rates of drug from both liposome types were comparatively similar. Dissolution rates of drugs with serum and liver homogenate were faster than without these material. After preparation of liposome, free drug was removed efficiency by Dowex 50W-X4. When liposome was freeze-dried and then rehydrated in the presence of Tr, characteristics of liposome were maintained well in MEL than MLV. Tr Was used successfully as a cryoprotectant in the process of freeze drying and the optimal ratio of Tr:Lipid was 4:1(g/g).

• 미생물학회지
• /
• 제28권1호
• /
• pp.35-40
• /
• 1990

Thiostrepton 내성 유전자(tsr)를 포함하는 multi-copy 재조합 플라스미드 pJY7J2의 제한효소 절단지도를 작성하였다. pJY, 712는 Streptomyces에서 넓은 host range를 나타내었으며 cloning 목적에 사용할 수 있는 단일 BgtIl 제한효소 인식부위를 갖고 있었다. 플라스미드 pJY 712는 lethal zygosis(Ltz+) 현상을 보였다. pJY 712의 혁질전환빈도는 S. lividans에서 $5.0\times 10^{4}$ TFU였다. pJY 712의 Bell 제한효소 인식부위에 tyrosmase 유전자(mel)를 삽입하여 플라스미드 PJY713을 제조하였다. met 유전자를 포함한 재조합 플라스미드 pJY 714는 pJY 713의 일부분(1.9kb BgllI-BelI 단편)을 제거하여 제고하였다.

• 한국산업융합학회 논문집
• /
• 제21권1호
• /
• pp.17-27
• /
• 2018

We propose a new approach to control a biped robot motion based on iterative learning of voice command for the implementation of smart factory. The real-time processing of speech signal is very important for high-speed and precise automatic voice recognition technology. Recently, voice recognition is being used for intelligent robot control, artificial life, wireless communication and IoT application. In order to extract valuable information from the speech signal, make decisions on the process, and obtain results, the data needs to be manipulated and analyzed. Basic method used for extracting the features of the voice signal is to find the Mel frequency cepstral coefficients. Mel-frequency cepstral coefficients are the coefficients that collectively represent the short-term power spectrum of a sound, based on a linear cosine transform of a log power spectrum on a nonlinear mel scale of frequency. The reliability of voice command to control of the biped robot's motion is illustrated by computer simulation and experiment for biped walking robot with 24 joint.

• 음성과학
• /
• 제14권4호
• /
• pp.131-144
• /
• 2007

In an effort to improve the performance of mel frequency cepstral coefficients (MFCC), we investigate the effects of varying the parameters for the filter banks and their associated windows on speech recognition rates. Specifically, the mel and bark scales are combined with various types of filter bank windows. Comparison and evaluation of the suggested methods are performed by two independent ways of speech recognition and the Fisher discriminant objective function. It is shown that the Hanning window based on the bark scale yields 28.1% relative performance improvements over the triangular window with the mel scale in speech recognition error rate. Further work on incorporating PCA and/or LDA would be desirable as a postprocessor to MFCC extraction.

• 한국음향학회지
• /
• 제20권2호
• /
• pp.10-15
• /
• 2001

본 논문에서 우리는 음향신호에서 음성과 음악을 분류하는 음성/음악 분류실험에 사용되는 특징들간의 상호조합을 비교하였다. 음향신호는 3가지 (음성, 음악, 음성+음악)와 2가지 (음성, 음악)로 분류하였다. 실험은 멜캡스트럼, 에너지, 영교차를 특징으로 사용하였고, 음성/음악 분류성능이 가장 좋은 특징간 상호조합을 모색하였다. 분류 알고리즘으로는 Gaussian Mixture Model (GMM)을 이용하였으며, GMM에 의한 데이터 모델링 전에 각기 다른 특징들을 하나의 특징공간에서 결합하였다. 실험결과 3가지 분류기준 적용시에는 멜캡스트럼, 영교차 조합이 가장 좋은 결과 (음성: 95.1％, 음악: 61.9％, 음성+음악: 55.5％)를 보였고, 2가지 분류기준 적용시에는 멜캡스트럼, 에너지 조합과 멜캡스트럼, 에너지, 영교차 조합이 가장 좋은 결과 (음성: 98.9％, 음악: 100％)를 보였다.

• 한국ITS학회 논문지
• /
• 제11권1호
• /
• pp.86-91
• /
• 2012

다양한 잡음환경에 노출되는 텔레메틱스 기반의 음성 통화 시스템에서 고품질의 통화 품질을 제공하기 위해서는 저연산량을 가지며 효과적으로 배경 잡음을 제거할 수 있는 잡음제거 알고리즘이 요구된다. 본 논문에서는 Mel-Filter 기반의 잡음제거 알고리즘을 제안하며, 제안된 알고리즘을 기존 잡음제거 알고리즘들과 비교하여 설명한다. 자동차 잡음과 배블 잡음 환경에서 잡음제거 알고리즘의 성능 측정 결과, 제안된 Mel-Filter 기반의 잡음제거 알고리즘이 기존 잡음제거 알고리즘들에 비해 비슷한 PESQ 성능에 적은 연산량을 가지는 장점을 가지고 있으며, 제안된 잡음제거 알고리즘이 텔레메틱스 단말기에서 효과적으로 잡음을 제거할 수 있음을 입증하였다.

• 한국자동차공학회논문집
• /
• 제19권1호
• /
• pp.109-116
• /
• 2011

To measure the traffic pollutants with high temporal and spatial resolution under real conditions a mobile emission laboratory (MEL) was designed and built in KIST with close-cooperation with KIMM and Yonsei university. The equipment of the mini-van provides gas phase measurements of CO, NOx, $CO_2$, THC (Total hydrocarbon) and number density & size distribution measurements of fine and ultra-fine particles by a fast mobility particle sizer (FMPS) and a condensation particle counter (CPC). The inlet sampling port above the bumper enables the chasing of different type of vehicles. This paper introduces the construction and technical details of the MEL and presents data from the car chasing experiment of diesel and CNG city bus. The dilution ratio was increased rapidly according to the chasing distance. Most particles from the diesel city bus were counted under 300 nm and the peak concentration of the particles was located between 40-60 nm. However, the most particles from the CNG city bus were nano particle counted under 50 nm.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.26-26
• /
• 2017

Seed germination stimulated by light is said to be photoblastism. Photoblastism has not been reported in cereal crops, especially in the rice, but Korean weedy rice was reported to have photoblastism and longer mesocotyl than cultivar. Photoblastic weedy rice (PBR) was used to identify QTLs for photoblastism and mesocotyl length. In previous works, QTLs for photoblastism, pbr1 and pbr12 were identified on chromosomes 1 and 12 using 124 F4 lines from a cross between Ilpum and PBR using bulked segregant analysis. Two QTLs for mesocotyl elongation, qMel-1 and qMel-3 were mapped on chromosomes 1 and 3 120 F8 lines from the same cross. Of interest, the RM8260-RM246 region of pbr1 overlapped with a region of qMel-1. To know whether these two QTLs are functionally related, 110 F3 lines were developed from a cross between Ilpum and CR7124. CR7124 having photoblastism and long mesocotyl was selected from 120 F8 lines. 95 F3 lines were measured for germination rate in a light and dark condition and mesocotyl length. Mesocotyl length and germination rate in the dark condition in F3 lines showed significant correlation (r = 0.7, P < 0.0001). 95 $F_3$ lines were genotyped with RM7419 on chromosome 1. ANOVA showed that RM7419 was tightly linked to QTLs for photoblastism as well as mesocotyl length on chromosome 1 (P < 0.0001) indicating the tight linkage of two QTLs. Fine mapping of the two QTL is underway to analyze their functional relationship.

• 한국음향학회지
• /
• 제18권1호
• /
• pp.78-85
• /
• 1999

본 논문에서는 현재 음성 인식에서 널리 사용되고 있는 멜켑스트럼의 성능 향상 방안을 제안한다. 일반적으로 멜켑스트럼은 인접한 필터간의 중심 간격과 필터의 대역폭이 일정한 critical band 필터들을 사용하여 구한다. 그러나 필터의 특성에 따라 멜켑스트럼의 값들이 달라지게 되고, 이에 따라 인식 성능도 변하게 된다. 본 논문에서는 삼각형과 사각형 모양의 critical band 필터를 사용하여 인접한 필터간의 중심 간격과 필터의 대역폭을 각각 변화시키면서 멜켑스트럼을 구하고 이에 따른 인식 성능을 분석한다. 또한 최적화 알고리즘인 simplex 방법을 사용하여 필터의 중심 주파수와 대역폭을 각각 변화시키면서 최적의 성능을 나타내는 필터를 구하는 방법을 제안한다. 인식 알고리즘으로 DTW (dynamic time warping)를 사용하고, 남자 10명과 여자 10명이 발음한 한국어 숫자음을 인식 대상으로 하여 실험을 수행하였다. 사각형 모양의 필터가 삼각형 모양의 필터 보다 우수한 성능을 보여 주었고 제안된 방법으로 최적화된 필터를 사용하여 구한 멜켑스트럼은 기존의 critical band 필터를 사용하는 것보다 향상된 인식 성능을 나타내었다.

• Animal cells and systems
• /
• 제1권1호
• /
• pp.143-150
• /
• 1997

Transcription factor CP2 was identified initially to bind the promoter region of the murine a-globin gene and its activity was shown to increase 2 to 3 fold during the induced differentiation of murine erythroleukemia (MEL) cells. To get further insight into the role of CP2 during development and differentiation, steady-state levels of CP2 message were monitored by using reverse transcriptase (RT)-PCR and in situ hybridization assays in the cultured MEL cells and differentiating embryonic stem (ES) cells in vitro, and in fetal and adult mouse tissues. The amount of CP2 messages increased 3 to 5 fold during induced differentiation of MEL cells, suggesting that the increment of CP2 activity during induced differentiation of MEL cells is originated from the increase of transcription initiation. On the other hand, CP2 expression is not restricted to the erythroid lineage cells; CP2 expressed ubiquitously from the undifferentiated ES cells to adult tissue cells. CP2 transcript was observed even in the undifferentiated ES cells and the level of expression increased from day 8 of the differentiating embryoid bodies. RT-PCR assay in the total RNAs prepared from several tissues of the adult mouse also showed ubiquitous expression profile, although the levels of expression were variable among tissues. When non-radioactive in situ hybridization assay was performed to the paraffin-sectioned whole body mouse embryos at days 11.5, 13.5, and 16.5 after fertilization, variable amounts of positive signals were also detected in different tissues.