• Title/Summary/Keyword: Medicinal resource

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Molecular Cloning and Characterization of the Gene Encoding Cinnamyl Alcohol Dehydrogenase in Panax ginseng C.A. Meyer (고려인삼으로부터 Cinnamyl Alcohol Dehydrogenase 유전자의 분리 및 특성)

  • Pulla, Rama Krishna;Shim, Ju-Sun;Kim, Yu-Jin;Jeong, Dae-Young;In, Jun-Gyo;Lee, Beom-Soo;Yang, Deok-Chun
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.4
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    • pp.266-272
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    • 2009

  • Cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.95), catalyzes the reduction of hydroxycinnamaldehydes to give hydroxycinnamyl alcohols, or "monolignols," the monomeric precursors of lignin. Lignins are important components of cell walls and lignified secondary cell walls play crucial roles in long distance transport of water and nutrients during plant growth and development and in plant defense against biotic and abiotic stresses. Here a cDNA clone containing a CAD gene, named as PgCAD, was isolated from a commercial medicinal plant Panax ginseng. PgCAD is predicted to encode a precursor protein of 177 amino acid residues, and its sequence shares high homology with a number of other plant CADS. The expression of PgCAD in adventitious roots and hairy roots of P. ginseng was analyzed using reverse transcriptase (RT)-PCR under various abiotic stresses such as salt, salicylic acid, wounding and chilling treatment that triggered a significant induction of PgCAD at different time points within 2-48 h post-treatment. This study revealed that PgCAD may help the plants to survive against various abiotic stresses.

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Characterizations of Broad Benn Wilt Virus isolated from Rehmania glutinosa Liboschitz

  • Park, In-Sook;Park, Min-Kyoung;Kim, Jong-Yeop;Lim, Ju-Rak;Lee, Kui-Jae;Lee, Wang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11b
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    • pp.54-54
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    • 2002

  • The nucleotide sequence of a Korea isolate of broad bean wilt favavirus from Rehmannia glutinosa Lib., designated BBWV-RE, was determined. Direct amino acid sequencing of the virus coat proteins suggests that a comparison of several favaviruses in terms of nucleotide sequence and amino acid sequences showed that BBWV-2 isolates display high sequence identity. The small coat protein genes of RNA-2 were also determined for three other Japanese isolates(E, L, and 1-2) and two ATCC isolates(PV132 and PV176) of BBWV. the CP sequence suggested distinct evolution lineages. Serotype 2 favaviruses are more prevalent in Asia, Australia and North America, Wheres serotype 1 is more prevalent in europe.(중략)

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