• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.3
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• pp.190-195
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• 2015

The detection of single nucleotide polymorphisms (SNPs) caused of mutant or genetic diseases is important to diagnosis and medicine. There are many methods have been proposed to detect SNPs. However the detection of SNPs is difficulty, because the difference of energy between complementary DNA (cDMA) and SNPs is very small. In this work, we detect the SNPs using field-effect transistors (FETs) which based on the detection of negative charge of DNA. We bias -0.3 V on the drain-source electrode at the target DNA hybridization process. The efficiency of hybridization and the amplitude of signal decrease by repulsive force between negative charge of DNA and negative bias on the electrode. However, the sensitivity of SNPs increases about 5 times from 1.7 mV to 8.7 mV.

• Korean Journal of Health Education and Promotion
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• v.1 no.1
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• pp.72-82
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• 1983

The purpose of this study is to provide the basic data necessary for the effective performance of administrative readjustment and demend and suppley medical care service by of analysing the extent of satisfaction of outpatient toward hospital. The subjects of this study are the 832 outpatients (398 male and 434 female) visit to 2 different university hospital in Seoul. The data were collected through self-administered techniques with a structured questionnaire from Oct.21 to Nov. 9, 1982. All the collected data were analyzed by means of percentage, mean and standard deviation. The results were as follows: 1. Those who are between :30 and 39 of age constitute the largest part of them as being 31.7 percent of the whole body. 40.0 percent of them graduated from the college and they take the lergest part of those who answered the questionnaire. 43.3 percent of the patients visit to the hospital by the reason for the reputation of a doctor and they take the largest part of the subjects. 2. The extent of satisfaction for hospital system. The mean extent of satisfaction for hospital system was revealed 2.50 scores, which is evaluated to neutral. The mean extent of satisfaction for waiting time of prescribed medicine presented 1.51 scores, the lowest among the component of hospital system, which is evaluated to high dissatisfaction. 3. The extent of satisfaction for the environment and facilities of hospital. The mean extent of satisfaction for the environment and facilities of hospital was revealed 3.08 scores, which is evaluated to moderate satisfaction. 4. The extent of satisfaction for doctor and other hospital employees. The mean extent of satisfaction for doctor and other hospital employees was revealed 3.05 scores which is evaluated to moderate satisfaction. The mean extent of satisfaction for doctor presented 3.39 scores, the highest among the components of doctor and other hospital employees. 5. The extent of satisfaction for charge of hospital. The mean extent of satisfaction for charge of hospital was revealed 2.74 scores, which is evaluated to neutral. 6. The extent of whole satisfaction for hospital. The mean extent of whole satisfaction for hospital was revealed 2.84 scores which is evaluated to neutral.

• Korea Journal of Hospital Management
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• v.20 no.3
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• pp.13-23
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• 2015

This research is intended to inquire any discrepancy depending on the variables such as types, geography and size of hospitals of Korea in order to develop differentiated marketing strategy and to investigate how the aforementioned variables affect the management performance such as the increase in the number of foreign patients, their willingness to re-visit, the increase in profit and etc. The survey for this research was conducted for five weeks from July 10th 2014 to August 30th 2014 with 251 participants in charge of foreign patient attraction for 161 domestic hospitals. As the method of the research, a hypothesis was first established based on previous studies, followed by the incorporation of self-administered questionnaire to confirm the formulated hypothesis. Frequency analysis and ANOVA analysis were used to analyze the result of the survey. The outcome of the research and the implications are as follows. First, according to the demography of the persons-in-charge of foreign patient attraction, the proportion of female was superbly high by 76.5%. As for the age, those in their 30s were highest in proportion. Finally, for the education level, college graduates took up the largest portion by 46.5%. Second, in respect to the hypothesis assuming the difference in performance depending on the types of hospitals, national university-affiliated hospitals showed the highest level of the increase in foreign patients by the average of 3.25. Third, in respect to the hypothesis assuming the difference in performance based on geographical position, it was confirmed that hospitals in Ulsan City experienced the largest growth in the number in foreign patients. Fourth, in respect to the sizes of hospitals, those with 201 to 300 sickbeds showed the highest increase by the average of 3.45. The implication of the above research outcome indicates that while the number of foreign patients visiting Korea for medical purposes is on the rise, the number of professionals in place with necessary knowledge and capacity is insufficient and requires improvement.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1299-1306
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• 2015

This study aims to investigate the mechanism of action of the cinnamon bark essential oil (CB), when used singly and also in combination with piperacillin, for its antimicrobial and synergistic activity against beta-lactamase TEM-1 plasmid-conferred Escherichia coli J53 R1. Viable count of bacteria for this combination of essential oil and antibiotic showed a complete killing profile at 20 h and further confirmed its synergistic effect by reducing the bacteria cell numbers. Analysis on the stability of treated cultures for cell membrane permeability by CB when tested against sodium dodecyl sulfate revealed that the bacterial cell membrane was disrupted by the essential oil. Scanning electron microscopy observation and bacterial surface charge measurement also revealed that CB causes irreversible membrane damage and reduces the bacterial surface charge. In addition, bioluminescence expression of Escherichia coli [pSB1075] and E. coli [pSB401] by CB showed reduction, indicating the possibility of the presence of quorum sensing (QS) inhibitors. Gas-chromatography and mass spectrometry of the essential oil of Cinnamomum verum showed that trans-cinnamaldehyde (72.81%), benzyl alcohol (12.5%), and eugenol (6.57%) were the major components in the essential oil. From this study, CB has the potential to reverse E. coli J53 R1 resistance to piperacillin through two pathways; modification in the permeability of the outer membrane or bacterial QS inhibition.

• Nuclear Engineering and Technology
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• v.43 no.6
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• pp.567-572
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• 2011

A sensitivity analysis of the methods used to evaluate the transport properties of a CdZnTe detector was performed using two different radiations (${\alpha}$ particle and gamma-ray) emitted from an $^{241}Am$ source. The mobility-lifetime products of the electron-hole pair in a planar CZT detector ($5{\times}5{\times}2\;mm^3$) were determined by fitting the peak position as a function of biased voltage data to the Hecht equation. To verify the accuracy of these products derived from ${\alpha}$ particles and low-energy gamma-rays, an energy spectrum considering the transport property of the CZT detector was simulated through a combination of the deposited energy and the charge collection efficiency at a specific position. It was found that the shaping time of the amplifier module significantly affects the determination of the (${\mu}{\tau}$) products; the ${\alpha}$ particle method was stabilized with an increase in the shaping time and was less sensitive to this change compared to when the gamma-ray method was used. In the case of the simulated energy spectrum with transport properties evaluated by the ${\alpha}$ particle method, the peak position and tail were slightly different from the measured result, whereas the energy spectrum derived from the low-energy gamma-ray was in good agreement with the experimental results. From these results, it was confirmed that low-energy gamma-rays are more useful when seeking to obtain the transport properties of carriers than ${\alpha}$ particles because the methods that use gamma-rays are less influenced by the surface condition of the CZT detector. Furthermore, the analysis system employed in this study, which was configured by a combination of Monte Carlo simulation and the Hecht model, is expected to be highly applicable to the study of the characteristics of CZT detectors.

• Health Policy and Management
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• v.28 no.1
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• pp.53-69
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• 2018

Background: This study investigates association modified category medical specialization (CMS) and hospital charge, length of stay (LOS), and mortality among lumbar spine disease inpatients. Methods: This study used National Health Insurance Service-cohort sample database from 2002 to 2013, using stratified representative sampling released by the National Health Insurance Service. A total of 56,622 samples were analyzed. The primary analysis was based on generalized estimating equation model accounting for correlation among individuals within each hospital. Results: Inpatients admitted with lumbar spine disease at hospitals with higher modified CMS had a shorter LOS (estimate, -1.700; 95% confidence interval [CI], -1.886 to -1.514; p<0.0001). Inpatients admitted with lumbar spine disease at hospitals with higher modified CMS had a lower mortality rate (odds ratio, 0.635; 95% CI, 0.521 to 0.775; p<0.0001). Inpatients admitted with lumbar spine disease at hospitals with higher modified CMS had higher hospital cost per case (estimate, 192,658 Korean won; 95% CI, 125,701 to 259,614; p<0.0001). However, inpatients admitted with lumbar spine surgery patients at hospitals with higher modified CMS had lower hospital cost per case (estimate, -152,060 Korean won; 95% CI, -287,236 to -16,884; p=0.028). Inpatients admitted with lumbar spine disease at hospitals with higher modified CMS had higher hospital cost per diem (estimate, 55,694 Korean won; 95% CI, 46,205 to 65,183; p<0.0001). Conclusion: Our results showed that increase in hospital specialization had a substantial effect on decrease in hospital cost per case, LOS, and mortality, and on increase in hospital cost per diem among lumbar spine disease surgery patients.

• Journal of the Korean Society of Industry Convergence
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• v.24 no.6_2
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• pp.809-818
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• 2021

The purpose of this study was to identify the influence of oral health knowledge and awareness of caregivers in charge on the oral health-related quality of life of the elderly in nursing homes. Data were collected from 115 elderly without dementia and their 115 caregivers in nursing facilities in S and C cities. The data were analyzed using SPSS/WIN 22.0 program. The average score for oral health knowledge and awareness of the caregivers were 11.62, 39.22 points each and the oral health-related quality of life of the elderly was 40.62 points. Oral health knowledge, awareness of caregivers and oral health-related quality of life of the elderly showed a difference according to oral health education experience of the caregivers (𝜌<.001), the nursing facility evaluation grade (𝜌=.016), and the oral health education experience (𝜌=.008), working hours of 40 hours or less per week of caregivers (𝜌=.008) each in order. The influencing factors on the oral health-related quality of life of the elderly were the oral health education experience, the working hours per week of the caregivers and the facility evaluation grade. This findings imply that developing customized program and the work environment improvement for caregivers should be considered to improve the oral health-related quality of life of the elderly in nursing homes.

• Korea Journal of Hospital Management
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• v.3 no.1
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• pp.192-218
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• 1998

This study is the positive one about the influence which the characteristics of medical consumers' perceived words-transmission information exercises on its acceptance level and its activities. The aim of this study was to investigate how the characteristics-reliability of perceived information, its directionity, and its availability-had an influence on the acceptance level and activities of words-transmission information. For the purpose of that aim, the positive research by questionnaire was applied. In addition, to collect necessary data, investigation was performed about 20 days from July 1st, 1938 to July 20th, 1938. As for the respondents' sampling in sample planning, the method of Simple Random Sampling was used laying stress on patients or their responsible person aged over 20s in second and third medical institutions. And the collected sample were 230 people. Of these, However, the number of sample used actually for this study analysis was 211 except 19 people who responded unfaithfully. The analysis of the data collected by the abovesaid investigation used SPSS/PC statistical package. The study result to have proved the hypothesis by experiment is as follows. First, as the result of having analysed the influence relations on the acceptance level and activities of words-transmission information among the factors of reliability about medical consumers' perceived information, I found that 'specialty' had an affirmative effect and there was a significant effect relations. Second, as the result of having made a multi-regression analysis of the effect relations on the acceptance level and activities of words-transmission information of the factors of directionity on medical consumers' perceived information, 'reliablity' was found as having a significant effect on the acceptance of words-transmission information. Third, 'importantness' was revealed as having a significant effect on the words-transmission activities in the acceptance level and activities of words-transmission information among the factors of reliability on medical consumers' perceived information. Fourth. for medical consumers there was a statistical correlation between the acceptance level and activities of words-transmission information. Therefore, words-transmission communication will be very useful for hospital promotion programs having a limited advertising ability in business. Especially, I suggest that a person in charge of his/her hospital marketing should not only excavate a source of words-transmission communication in order to arrive effectively at sampling market, but also use a marketing mix suitable to that The test result shows that the unsatisfied patients over their hospital treatment tend to be more stronger in transmitting words to the other positive minded patients than the satisfied ones. Therefore, all relating representatives in hospital should concentrate their energies on decreasing or eliminating these unwanted results.

• Journal of Periodontal and Implant Science
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• v.42 no.3
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• pp.95-104
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• 2012

Purpose: The purpose of this study was to determine whether increasing the Ti6Al4V surface oxide negative charge through heat ($600^{\circ}C$) or radiofrequency plasma glow discharge (RFGD) pretreatment, with or without a subsequent coating with fibronectin, stimulated osteoblast gene marker expression in the MC3T3 osteoprogenitor cell line. Methods: Quantitative real-time polymerase chain reaction was used to measure changes over time in the mRNA levels for osteoblast gene markers, including alkaline phosphatase, bone sialoprotein, collagen type I (${\alpha}1$), osteocalcin, osteopontin and parathyroid hormone-related peptide (PTH-rP), and the osteoblast precursor genes Runx2 and osterix. Results: Osteoprogenitors began to differentiate earlier on disks that were pretreated with heat or RFGD. The pretreatments increased gene marker expression in the absence of a fibronectin coating. However, pretreatments increased osteoblast gene expression for fibronectin-coated disks more than uncoated disks, suggesting a surface oxide-mediated specific enhancement of fibronectin's bioactivity. Heat pretreatment had greater effects on the mRNA expression of genes for PTH-rP, alkaline phosphatase and osteocalcin while RFGD pretreatment had greater effects on osteopontin and bone sialoprotein gene expression. Conclusions: The results suggest that heat and RFGD pretreatments of the Ti6Al4V surface oxide stimulated osteoblast differentiation through an enhancement of (a) coated fibronectin's bioactivity and (b) the bioactivities of other serum or matrix proteins. The quantitative differences in the effects of the two pretreatments on osteoblast gene marker expression may have arisen from the unique physico-chemical characteristics of each resultant oxide surface. Therefore, engineering the Ti6Al4V surface oxide to become more negatively charged can be used to accelerate osteoblast differentiation through fibronectin-dependent and independent mechanisms.

• Journal of mucopolysaccharidosis and rare diseases
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• v.3 no.1
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• pp.20-27
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• 2017

Purpose: Mucopolysaccharidosis IV (MPS IV) is a disease characterized by deficient activity of N-acetylgalactosamine-6-sulfatase (GALNS) causing excessive lysosomal storage of keratan sulfate (KS). The identification of the relevant disaccharide units of KS after keratanase II digestion followed by liquid chromatography/tandem mass spectrometry detection (LC-MS/MS) is validated and applicable for the preliminary diagnosis of MPS IV. Methods: A total of 67 urine samples were collected and analyzed from 11 MPS IV patients comprising 10 MPS IVA and one MPS IVB patients, and 56 normal controls. Urinary glycosaminoglycan was first precipitated by the Alcian blue method followed by a digestion of keratanase II. The protonated species of the digested disaccharide products were detected by using multiple reaction monitoring experiment. Results: One particular disaccharide of KS was selected. The transition mass-to-charge (m/z) of the parent ion and its daughter ion after collision was $462.0{\rightarrow}97.0$, whereas the chondrosine used as an internal standard in this assay was m/z $353.9{\rightarrow}73.0$. The results corresponded well with the two-dimensional electrophoresis method. The quantities of urinary KS were significantly raised in confirmed MPS IV patients when comparing with those of normal controls ($170.2{\pm}81.1$ vs. $4.06{\pm}1.92{\mu}g/mL$). Conclusion: The LC-MS/MS method for MPS IVA determination is specific, sensitive, validated, and applicable for urinary KS quantification. This method can be used not only as a first-line biochemistry examination of MPS IVA, but also as an outcome survey after enzyme replacement therapy.