• 동의생리병리학회지
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• 제21권5호
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• pp.1081-1086
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• 2007

This study was performed to investigate the medicinal effects of the herbal combination extracts-2 (HCE-2), consisting of Artemisia capillaris Thunb., Lonicera japonica Thunb., Prunella vulgaris var. lilacina, and Hovenia dulcis Thunb. on the alcohol-induced liver injury in rats. The rats were randomly divided into four groups: normal group (n =6), non-treated control group (n =6), saline-treated group (n =6) and the herbal combination extract (HCE-2)-treated group (n =6). The rats in the alcohol-loaded groups were orally administered with ethanol at a daily dose of 4 g/kg-body weight for 5 weeks. Thirty minutes before the ethanol injection, saline or herbal combination extracts was administered by using a gastrogavage. Blood and liver tissue samples were taken out from the hearts and livers of the rats, respectively, on 15th and 38th days. The activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured using an enzyme-linked immunosorbent assay (ELISA). We also investigated the protective effect of the herbal combination extracts by Hematoxylin-Eosin staining on histological sections of rat liver. In this study, the oral administration of the herbal combination extracts significantly reduced the serum levels of AST and ALT, which had been raised by alcohol-induced liver injury. Histological analysis and apparent observation of liver also showed the preventive effect of the herbal combination extracts in a chronic alcohol-induced rat model. Theses results revealed that the herbal combination extracts effectively prevented hepatic damage consequent to the chronic exposure to repetitive administration of ethanol and could be used as a primary resource of a health beverage or herbal medicine, alleviating the alcohol-induced hepatic injury and hangover symptoms.

• Journal of Preventive Medicine and Public Health
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• 제26권4호
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• pp.587-598
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• 1993

Hydrofluoric acid is one of the strongest irritating, corrosive and poisonous inorganic chemicals. Hydrofluoric acid burns are occurring with ever-increasing frequency due to the wide use of this acid in industries. Hydrofluoric acid burns are characterized by severe progressive tissue destruction and excruciating pain due to the unique properties of the freely dissolvable fluoride ion. The authors reviewed medical records of 32 cases (36 spells) of hydrofluoric acid burns which occurred in a hydrofluoric acid manufacturing factory from Sep. 1, 1990 to June 30, 1993. The results are as follows; 1. Eleven measurements of air concentrations of hydrofluoric acid by detection tube method from 1990 to 1992 were all below TLV (Department of Labor, R.O. K). 2. There were 19 cases (22 spells) of hydrofluoric acid burns which occurred during the study period among regular employees. The overall incidence density of hydrofluoric acid was 17.8 cases (20.6 spells) per 100 person-year. Incidence density was 19.0 cases (22.0 spells) per 100 person-year among male workers and there were no female cases. Incidence density was 32.9 cases (38.3 spells) per 100 person-year among production workers and 1.9 cases (1.9 spells) per 100 person-years among management workers with the difference being statistically significant (P<0.01). 3. Of 32 cases (36 spells) of hydrofluoric acid burns among workers who were regularly employed or temporarily employed, 26 spells (81.2%) were between age 20 to 39. In 15 spells(41.7%) burns occurred between 12:00 and 17:59 with 16 spells(44.3%) having arrived at hospital within 2 hours after the accident. 4. Of 36 spells, the main cause of hydrofluoric acid burns were by splashes (8 spells, 22.2 %). The most frequent site of burns were fingers and pain was the most frequent symptom. Thirty spells (83.3%) of the hydrofluoric acid burns were treated with local injection of antidote (calcium gluconate). Complete recovery without scarring were observed in most of the cases (34 out of 36 cases, 94.4%). The study results suggest that to prevent hydrofluoric acid burns, environmental control and the wearing of hydrofluoric acid resistant protective clothes and gloves are important. It is also stressed that establishment of an emergency management and a transfer system for hydrofluoric acid burn victims is necessary.

• 대한방사선기술학회지:방사선기술과학
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• 제38권2호
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• pp.115-120
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• 2015

Gadoxetic acid (GA) 조영증강 자기공명(MR) 영상에서 일반적으로 많이 이용되는 숙임각(flip angle, FA) $11^{\circ}$와 비교하여 FA $30^{\circ}$를 이용한 3분, 10분 및 15분 영상에서 국소성 간 병변 검출 차이를 알아보고자 하였다. 대상 및 방법 : 3.0T MR 기기로 GA 조영증강지연기 MR영상을 시행 받은 69명의 환자를 대상으로 하였다. 대상 환자는 간세포암 23명과 전이암 12명으로 총 35명(남성 23, 여성12, 평균연령 60.4세)이었다. GA 주입 후 3분, 10분, 15분의 영상에서 각각 FA $11^{\circ}$와 $30^{\circ}$ 영상을 획득하였다. 각각의 영상에서 정량적평가와 정성적평가를 한 뒤 독립표본 T검정을 이용하여 통계적 분석을 하였다. 정량적 평가와 정성적 평가 모두 조영제 주입 후 3분과 10분 영상에서 FA $30^{\circ}$ 영상이 FA $11^{\circ}$ 영상보다 약간 우수하였으나 통계적 유의성은 없었다. 그러나 15분 영상에서는 FA $30^{\circ}$ 영상이 FA $11^{\circ}$ 영상보다 통계적으로 유의하게 우수하였다(p<0.05). GA 조영증강 후 15분 MR영상에서 FA $30^{\circ}$ 영상은 기존의 FA $11^{\circ}$ 영상보다 간 병변을 민감하게 검출해낼 수 있다.

• 대한한의학회지
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• 제16권1호통권29호
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• pp.379-402
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• 1995

This study was performed to investigate the comparative effects of Apitoxin(0.1% bee venom 0.1cc) & Acupuncture therapy on the knee joint arthritis induced by 0.1% carrageenin solution at Rt. knee joint in rats. After carrageenin injection, the apitoxin was injected for two times (Experimental group I : 1 & 24hours later, Experimental group II : 48 & 72hours later) into the corresponding loci to Rt. $ST_{35}$(Group 3) & $EX-LE_4$(group 4) of the human body in rats. Acupuncture therapy was done same as above. And then the comparative effects of apitoxin and acupuncture therapy on the knee joint arthritis were estimated by the WBC count, RBC count, hemoglobin level, hematocrit level and ASO titer in serum. The results were summerized as follows : 1. The effects of apitoxin & acupuncture on WBC count showed remarkable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 2. The effect of apitoxin on RBC count showed noticeable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. The acupuncture was not showed decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 3. The effects of apitoxin & acupuncture on hemoglobin level showed noticeable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 4. The effect of apitoxin on hematocrit level showed noticeable decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. The acupuncture was not showed decrease at group 3 & group 4 as compared with the control group in both experimental group I & experimental group II. There were not any statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. 5. The effect of apitoxin on ASO titer showed noticeable decrease at group 3 & group 4 as compared with the control group only in experimental group I. The apitoxin & acupuncture showed decrease at group 3 & group 4 as compared with the control group in experimental group II. There were significantly statistical difference from apitoxin and acupuncture therapy by Duncan's multiple range test. The effect of apitoxin showed slightly decrease as compared with the acupuncture therapy by Duncan's multiple range test.

• Radiation Oncology Journal
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• 제12권3호
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• pp.301-305
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• 1994

Purpose :. To investigate the effect of aqueous extract of Ganoderma lucidum(G.I.) on the surival of tumor cells in vitro and on the growth of tumors in vivo. Materials and Methods : Dried G.I. was made into powder, extracted with distilled water, filtered and diluted from a maximum concentration of 100 mg/ml in sequence. The cytotoxicity of G.I, in vitro was evaluated from its ability to reduce the clonogenicity of SCK tumor cells. For the tumor growth delay study, about $2{\times}10^5$$ of SCK tumor cells were subcutaneously inoculated in the legs of A/J mice. The first experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. from the first day after tomor inoculation for 10 days. The second experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. either once a day for 10 days or twice a day for 5 days beginining from the 7th day after tumor inoculation Results : 1. Cytotoxicity in vitro;survival fraction, as judged from the curve, at G.I. concentration of 0.5, 1,5, 10, 25, 50 and 100 mg/ml were 1.0, $0.74{\pm}0.03$, $0.18{\pm}0.03$, $0.15{\pm}0.02$, $0.006{\pm}0.002$, 0.015 and 0.0015, respectively. 2. Tumor growth delay in vivo; a) the time required for the mean tumor volume to grow to $1,000mm^3$ was 11 days in the control group and 14 days in the experimental group. b) the time required for tumor volume to increase 4 times was 11 days in the control group while it was 10.5 and 12 days in the groups injected with G.I. once a day and twice a day from the 7th day after tumor inoculation respectively. Conclusion : Aqueous extracts of G.I. showed a marked cytotoxicity on the SCK mammary cells in vitro. Tumor growth delay was statistically signiricant when G.I. in-jection was started soon after tumor inoculation, but it was not significant when injection was started after the tumors were firmly established.

• Journal of Chest Surgery
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• 제39권5호
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• pp.394-398
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• 2006

배경: 원발성 자연기흉에 대한 초 치료의 목적은 흉막강 내의 공기 제거와 폐장의 재팽창 유도이며, 이를 위해 통상적으로 20 Fr이상 크기의 흉관을 흉막강에 삽입한다. 그러나 원발성 자연기흉의 병태 생리를 고려할 때보다 작은 크기의 흉관으로도 소기의 목적을 달성할 수 있다는 생각에서, 7 Fr 크기의 도관을 흉관으로 사용하여 그 효용성과 보완점을 제시하고자 하였다. 대상 및 방법: 2003년 5월부터 2005년 4월까지 원발성 자연기흉으로 입원 치료를 한 환자 중 초 치료로 흉강삽관술을 시행한 111명의 환자를 대상으로 의무기록을 토대로 후향적으로 조사하여, 7 Fr 흉관을 사용한 환자들과 24 Fr 흉관을 사용한 환자들의 흉관 거치기간, 진통제의 사용여부, 폐의 재팽창 정도, 합병증 등에 대해 분석하였다. 결과: 흉관의 평균 거치기간은 7 Fr군이 $2.4{\pm}1.1$일, 24 Fr군이 $2.3{\pm}1.3$일로 차이가 없었다. 주사 진통제의 사용은 7 Fr군에서는 없었으나 24 Fr군에서는 모든 환자들이 사용하였다. 7 Fr 흉관만을 사용하여 치료한 환자들의 77%에서 폐장의 완전 재팽창을 얻을 수 있었다. 7 Fr 흉관의 문제점은 도관의 꺾임이며, 5.6%에서 발생하였다. 결론: 7 Fr 흉관을 이용한 원발성 자연기흉의 초 치료는 기존의 24 Fr 흉관과 비교하여 공기제거, 폐의 재팽창, 통증경감, 미용적 측면에서 모두 만족할 만 하였으나, 꺾임을 방지할 수 있게 도관의 개량이 필요하다고 생각한다.

• 한국산학기술학회논문지
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• 제19권8호
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• pp.417-421
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• 2018

카르복시테라피(Carboxytherapy)는 메조테라피(mesotherapy)와 방법, 치료 조건 및 결과 면에서 매우 유사하다. 그러나 중요한 차이점은 카르복시테라피는 이산화탄소 가스를 피부 바로 아래의 피하층으로 주입하는 반면 메조테라피는 비타민, 미네랄, 그리고 약물의 혼합물을 주입한다는 것이다. 메조테라피의 합병증으로 혈종, 파누스육아종, 봉와직염, 농양 등 이전에 많은 사례들이 보고되었으나, 카르복시테라피는 피하 및 얕은층에 이산화탄소를 주입하고, 이산화탄소는 주입 1주일 안에 흡수되기 때문에 부작용이 거의 보고 되지 않았다. 3일전과 2주전에 한의원에서 카르복시테라피를 받은 29세 여자 환자가 고열, 근육통, 심한 요통 및 엉덩이 부위부터 아래쪽 흉벽까지 피하 기종을 주소로 응급실에 내원하였다. 진단 및 치료를 위해 컴퓨터 단층 촬영을 시행하였으며, 즉시 영상의학과에 판단을 의뢰하였다. 바늘에 의한 손상으로 인한 급성 복막염을 배제할 수 없어 즉각적인 광범위항생제를 투여하였으며, 판독 결과 근육염과 바늘 손상으로 인한 신우신염이 확진되었다. 신우 신염의 경우 적절한 치료가 이루어지지 않으면 패혈증까지 진행할 수 있어 치명적인 결과를 초래 할 수 있다. 따라서 만일 바늘에 찔리거나 침을 맞은 후에 환자가 늑골척추부위나 허리에 통증이 있는 경우 응급의학과 의사는 진단 및 치료를 위해 신속한 초기 평가가 필요하다.

• Journal of Pharmaceutical Investigation
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• 제35권6호
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• pp.423-429
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• 2005

A selective and sensitive reversed-phase HPLC method for the determination of fenoprofen in human serum was developed, validated, and applied to the pharmacokinetic study of fenoprofen calcium. Fenoprofen and internal standard, ketoprofen, were extracted from human serum by liquid-liquid extraction with diethyl ether and analyzed on a Luna C18(2) column with the mobile phase of acetonitrile-3 mM potassium dihydrogen phosphate (32:68, v/v, adjusted to pH 6.6 with phosphoric acid). Detection wavelength of 272 nm and flow rate of 0.25 mL/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^{3}$ factorial design using a fixed fenoprofen concentration $(2\;{\mu}g/mL)$ with respect to its peak area and retention time. And also, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of $0.05-100\;{\mu}g/mL$ with correlation coefficients greater than 0.999. The lower limit of quantification using 1 mL of serum was $0.05\;{\mu}g/mL$, which was sensitive enough for pharmacokinetic studies. The overall accuracy of the quality control samples ranged from 92.27 to 109.20% for fenoprofen with overall precision (% C.V.) being 5.51-11.71 %. The relative mean recovery of fenoprofen for human serum was 81.7%. Stability (freeze-thaw, short and long-term) studies showed that fenoprofen was not stable during storage. But, extracted serum sample and stock solution were allowed to stand at ambient temperature for 12 hr prior to injection without affecting the quantification. The peak area and retention time of fenoprofen were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of fenoprofen in human serum samples for the pharmacokinetic studies of orally administered Fenopron tablet (600 mg as fenoprofen) at three different laboratories, demonstrating the suitability of the method.

• 한국산학기술학회논문지
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• 제19권2호
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• pp.329-338
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• 2018

치과위생사의 근골격계 질환을 조사하고, 통증 조절과 자기효능감이 근골격계 질환에 미치는 영향을 분석하고자, 광주지역 치과위생사 483명을 편의 추출하여 자기기입식 설문조사를 시행하고 빈도분석, 교차분석, t-검정, 다중로지스틱 회귀분석을 실시하여 분석하였다. 연구결과 연구 대상자의 지난 1년간 근골격계 통증 유병율은 83.9%이었으며, 이중 근골격계 질환이 있는 경우는 29.8%이었다. 근골격계 질환에 영향을 주는 요인을 파악한 결과, 근골격계 통증 해결 방법이었다. 통증 해결 방법에서 병가, 작업전환이라고 응답한 군에 비해 병원, 한의원, 약국치료 라고 응답한 군의 근골격계 질환 교차비(odds ratio)는 0.22(95% 신뢰구간 0.14-0.34)로 음의 관련성을 보였다. 이것은 근골격계 장애가 있는 경우 병원, 한의원, 약국치료를 더 하지 않은 것으로 사료된다. 따라서 치과위생사의 근골격계 통증 관리를 위해서는 의학적 관리(한의학적 치료, 정맥주사나 약물 치료, 열과 냉동 치료, 스테로이드 치료, 상담 치료, 척수자극을 이용한 중재적 치료, 물리 치료, 스트레칭, 재활운동치료, 도수 치료)와 작업환경 요인을 개선할 필요가 있고 근골격계질환 예방법에 대한 적절한 접근이 필요할 것으로 생각된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.265-271
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• 2014

Interleukin-7 (IL-7) is a potent anti-apoptotic cytokine that enhances immune effector cell functions and is essential for lymphocyte survival. While it known to induce differentiation and proliferation in some haematological malignancies, including certain types of leukaemias and lymphomas, little is known about its role in solid tumours, including breast cancer. In the current study, we investigated whether IL-7 could enhance the in vivo antitumor activity of tumor-reactive $CD8^+$ T cells with induction of IFN-${\gamma}$ in a murine breast cancer model. Human IL-7 cDNA was constructed into the eukaryotic expression plasmid pcDNA3.1, and then the recombinational pcDNA3.1-IL-7 was intratumorally injected in the TM40D BALB/C mouse graft model. Serum and intracellular IFN-${\gamma}$ levels were measured by ELISA and flow cytometry, respectively. $CD8^+$ T cell-mediated cytotoxicity was analyzed using the MTT method. Our results showed that IL-7 administration significantly inhibited tumor growth from day 15 after direct intratumoral injection of pcDNA3.1-IL-7. The anti-tumor effect correlated with a marked increase in the level of IFN-${\gamma}$ and breast cancer cells-specific CTL cytotoxicity. In vitro cytotoxicity assays showed that IL-7-treatment could augment cytolytic activity of $CD8^+$ T cells from tumor bearing mice, while anti-IFN-${\gamma}$ blocked the function of $CD8^+$ T cells, suggesting that IFN-${\gamma}$ mediated the cytolytic activity of $CD8^+$ T cells. Furthermore, in vivo neutralization of $CD8^+$ T lymphocytes by CD8 antibodies reversed the antitumor benefit of IL-7. Thus, we demonstrated that IL-7 exerts anti-tumor activity mainly through activating $CD8^+$ T cells and stimulating them to secrete IFN-${\gamma}$ in a murine breast tumor model. Based on these results, our study points to a potential novel way to treat breast cancer and may have important implications for clinical immunotherapy.