• Journal of Embryo Transfer
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• v.24 no.2
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• pp.97-104
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• 2009

The objective of this study was to examine the effect of macromolecule in a maturation medium on nuclear maturation, intracellular glutathione (GSH) level of oocytes, and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Immature pig oocytes were cultured in maturation medium that was supplemented with each polyvinyl alcohol (PVA), pig follicular fluid (pFF) or newborn calf serum (NBCS) during the first 22 h and the second 22 h. Oocyte maturation was not influenced by the source of macromolecules during in vitro maturation (IVM). Embryo cleavage and cell number in blastocyst after PA was altered by the source of macromolecule but no difference was observed in blastocyst formation among treatments. Oocytes matured in PVA-PVA medium showed lower rates of oocyte-cell fusion (70.4% vs. 77${\sim}$82%) and embryo cleavage (75% vs. 86${\sim}$90%) after SCNT than those matured in other media but blastocyst formation was not altered (13${\sim}$27%) by different macromolecules. pFF added to IVM medium significantly increased the intracellular GSH level of oocytes compared to PVA and NBCS, particularly when pFF was supplemented during the first 22 h of IVM. Our results demonstrate that source of macromolecule in IVM medium influences developmental competence of oocytes after PA and SCNT, and that pFF supplementation during the early period (first 22 h) of IVM increases intracellular GSH level of oocytes.

• The Research Journal of the Costume Culture
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• v.18 no.5
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• pp.991-1002
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• 2010

Nowadays, personal media is a new tool for communication as digital cameras and mobile phones are developing rapidly. We are concerned over Cyworld users who could have different personal characteristics which will influence on buying patterns in on line shopping behaviors. The purpose of this research was to observe fashion attitudes and purchase behavior of Cyworld mini homepage users, for establishing marketing strategies by understanding consumers. For this study, one line survey was used for 500 male and female subjects who are 20 to 40 years old. Only reliable 441 questionnaires were used for analysis. The SPSS program was used for frequency, K-means cluster, t test, and chi-square test. A total of 441 respondents were clustered on the basis of 8 item self-disclosure scale, using the K-means procedures. The results indicated that respondents were clustered into two segments; 267 respondents(active attitude towards self-disclosure) and 164 ones(not active). We examined fashion attitudes in mini home page and buying behavior by self-disclosed variable. Those who are involved actively in self expression and self-disclosure considered more fashion style and trend. The major motivates of web surfing was finding a good design, and good price. High self-disclosure group tends to search many shopping mall for right design and low self disclosure group tends to search them for the right price. High self-disclosure group tend to shop the fashion products more, while low self disclosure group tend to purchase books more through the internet. We realized that active group in self-disclosure purchased their clothing accidently when they visit Cyworld.

• Journal of Microbiology
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• v.44 no.3
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• pp.293-300
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• 2006

The Escherichia coli heat-labile enterotoxin B subunit (HLT-B) is one of the most powerful mucosal immunogens and known mucosal adjuvants. However, the induction of high levels of HLT-B expression in E. coli has proven a difficult proposition. Therefore, in this study, the HLT-B gene was cloned from pathogenic E. coli and expressed as a fusion protein with GST (glutathion S-transferase) in E. coli BL2l (DE3), in an attempt to harvest a large quantity of soluble HLT-B. The culture conditions, including the culture media used, temperature, pH and the presence of lactose as an inducer, were all optimized in order to obtain an increase in the expression of soluble GST-rHLT-B. The biological activity of the purified rHLT-B was assayed in a series of GMI-ELISA experiments. The findings of these trials indicated that the yield of soluble recombinant GST-rHLT-B could be increased by up to 3-fold, as compared with that seen prior to the optimization, and that lactose was a more efficient alternative inducer than IPTG. The production of rHLT-B, at 92 % purity, reached an optimal level of 96 mg/l in a 3.7 L fermentor. The specific GM1 binding ability of the purified rHLT-B was determined to be almost identical to that of standard CTB.

• Journal of Digital Convergence
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• v.14 no.12
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• pp.267-281
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• 2016

This study analyzed the contents of articles on GMO in Chosun Ilbo and Hankyoreh from 1994 through 2015 to check the trend of reporting on GMO as a science and technology risk issue. As a result, 'risk' and 'anxiety/concern' were continuously treated as important subjects, and there was a lack of depth and journalist's professionalism in all three periods: 'Introduction of GMO technology (1994-2000)', 'Development of GMO technology (2001-2010)' and 'Social acceptance of GMO (2011-2015).' In a comparison between the media with different ideological goals, the Chosun Ilbo had a stronger new-technological inclination to GMO than the Hankyoreh in reporting topics and tones of arguments. As a result of checking the relation of the tones in reporting with the 'risk-benefit' of GMO, reports in negative tones underlined 'risk' while those in positive tones, 'benefit.' This study could suggest improvements of subject bias and unprofessionalism in the domestic science journalism, the barometer of public awareness of disputes over science and technology risk.

• The Journal of the Korea institute of electronic communication sciences
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• v.12 no.6
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• pp.1135-1142
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• 2017

In this paper, we propose an algorithm that can automatically mix the screen and sound by tracking the change of the sound data according to the screen change so that the real sound can be implemented in the personal broadcasting service. Since the personal broadcasting service is often broadcasted lively, it should be convenient to have a real-time mixing. Through experiments, it was confirmed that the sound pressure changes in a wide range in the high frequency band related to the clarity for understanding according to the rotation angle change of the screen. Regression analysis of the sound pressure changes at 2kHz, 4kHz, and 8kHz, The attenuation change of sound pressure was observed at the slope of -1.17, the slope of -2.0, and the slope of -2.44 for each frequency. Therefore, these experiment results can be applied to the VR service. This study is expected to be useful data in the implementation of personal broadcasting service.

• Journal of Broadcast Engineering
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• v.13 no.2
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• pp.236-244
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• 2008

We propose a new building detection and description algorithm for Lidar data and photogrammetric imagery using color segmentation, line segments matching, perceptual grouping. Our algorithm consists of two steps. In the first step, from the initial building regions extracted from Lidar data and the color segmentation results from the photogrammetric imagery, we extract coarse building boundaries based on the Lidar results with split and merge technique from aerial imagery. In the secondstep, we extract precise building boundaries based on coarse building boundaries and edges from aerial imagery using line segments matching and perceptual grouping. The contribution of this algorithm is that color information in photogrammetric imagery is used to complement collapsed building boundaries obtained by Lidar. Moreover, linearity of the edges and construction of closed roof form are used to reflect the characteristic of man-made object. Experimental results on multisensor data demonstrate that the proposed algorithm produces more accurate and reliable results than Lidar sensor.

• KIPS Transactions on Computer and Communication Systems
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• v.2 no.8
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• pp.357-368
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• 2013

Our society has evolved into a fully connected society in a mixed reality environment enabling various knowledge sharing / management / control / creation due to the expansion of broadband ICT infrastructure, smart devices, cloud services and social media services. Therefore cyber threats have increased with the convenience. The society of the future can cause more complex and subtle problems, if you do not have an effective response to cyber threats, due to fusion of logical space and physical space, organic connection of the smart object and the universalization of fully connected society. In this paper, we propose the strategy to build knowledge-base as the basis to actively respond to new cyber threats caused by future various environmental changes and the universalization of fully connected society.

• The Journal of the Korea Contents Association
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• v.19 no.8
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• pp.483-489
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• 2019

Recently, various media such as broadcasting, movies, and mobile converge with game elements, expanding the contents and expanding the area. This study focuses on the gamification convergence contents. Among them, we examine the case of mobile application live quiz show by applying the formal elements of the game mentioned by Tracy Fullerton. The elements have all the formal elements of the game, which led to the conclusion that they lead the audience to immersion based on the play characteristics of the game. This can be attributed to the elements of the game that have penetrated into the contents, and it can be reinterpreted, and the directions of new contents can be presented, which is industrially, culturally and academically significant. This study focuses on the industrial and cultural achievements created by contents, and plans and prospects new content that balances play and usefulness.

• Journal of Korea Society of Industrial Information Systems
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• v.24 no.3
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• pp.39-50
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• 2019

Social commerce is a term derived from the fusion of social media and e-commerce. This study aims to classify social commerce benefits as social benefits, confidentiality benefits, economic benefits, and customization benefits based on previous research claims. We also examine the effect of social commerce relationship benefits on trust and flow, and the effect of trust and flow on customer loyalty. Also, we examine how the relationship between trust and customer loyalty, and commitment and customer loyalty varies with self determination. The following are main results of this study. First, social benefits, confidentiality benefits, economic benefits, and customization benefits have significant effects on trust and flow. Second, social commerce trust and flow have a positive effect on customer loyalty. Third, the relationship of social commerce trust, flow, customer loyalty is differ depending on self determination. This study suggests the necessity of differential approach according to self-determination.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.18 no.1
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• pp.83-90
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• 2019

LED lighting is spreading on the strength of LED lighting, and various government policies are being implemented. VLC research which is a wireless communication technology using lighting has been actively conducted, and it has been proven through many studies that a general LED light source such as a high-speed data transmitter can be used. But from now on, one of the main problems is the noise from side lights, which can be compared to the noise of radio broadcasts. So in this paper, we proposed a noise canceling model to remove the interference of ambient light by using an optical filter for a detachable VLC channel. In order to verify the proposed model, various high brightness RGB LED modules were used for comparative analysis. In addition, the applicability was verified through experiments using High Luminance LED lighting which is applied in real life.