• The Journal of the Korean Society for Microbiology
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• v.22 no.3
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• pp.197-208
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• 1987

Chlamydia trachomatis has now shown that this interesting intracellular parasite is a cause of nongonococcal urethritis, infantile pneumonia, pelvic inflammatory disease and epididymitis, in addition to lymphogranuloma venerum and inclusion conjunctivitis. There are several diagnostic methods for C. trachomatis, but the method using monoclonal antibody is the most sensitive and specific. The hybride cell were prepared by fusion of myeloma cell($P_3X_{63}\;Ag_8{\cdot}V_{653}$) of mouse and lymphocyte of mouse(BALB/c) that were immunized with formalin killed C. trachomatis serotype D. The cell mixtures after fusion were dispensed into 640 wells of the 96 well culture plates and continuously cultured in HAT medium for 2 weeks. The supernatants of culture media in 83(13%) wells were reacted with C. trachomatis, which were determined by enzyme-linked immunosorbent assay in 96 well microplate. The clones that secreted antibody to C. trachomatis were cloned by limiting dilution. Only six monoclones secreted antibody to C. trachomatis. The antibody titer of ascitic fluid that collected from same BALB/c mice bearing hybridoma cells was above 1:100,000. These monoclonal antibodies that were IgG reacted with elementary and reticulate bodies of all serotypes(Ba, D, E, F, G, H, J and LGV type-I) using ELISA and indirect immunofluorescence stain, but there were no cross reaction with other bacteria(coagulase negative Staphylococcus, Proteus and E. coli). We concluded these six monoclones secreted the same monoclonal antibody to C. trachomatis. The sensitivity and specificity of the monoclonal antibody compared with Microtrak(confirmatory test of C. trachomatis, Syva) was 100%, respectively.

• Journal of Life Science
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• v.12 no.1
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• pp.55-60
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• 2002

The differentiation of skeletal muscle precursor cells in culture is marked by the transcriptional activation of muscle-specific genes and the morphological differentiation of myoblast into multinucleate myotube. In this study, we examined the effect of TSA (Trichostatin A) on WF-kB DNA binding activity and muscle cell fusion in the process of myogenesis. Under TSA treatment, C2C12 myoblast could not fuse to myotube and its NF-kB DNA binding activity was also blocked. To investigate whether these phenomenons were affected by TSA in direct or not, differentiation media (DM) used to differentiate cells without TSA was concentrated and added to C2C12 myoblast with TSA simultaneously. C2C12 myoblast was fused to myotube and NF-kB DNA binding activity was recovered. These results suggest that TSA affects on the differentiation of myoblast, perhaps through several factors, by inhibiting myoblst fusion and blocking NF-kB DNA binding activity.

• Journal of Broadcast Engineering
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• v.26 no.4
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• pp.390-397
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• 2021

As digital twin technology is applied to various industrial fields, technologies to effectively process large amounts of data are required. In this paper, we discuss a customized service method for fast search and effective delivery of large-scale data for underground facility for public utilities management. The proposed schemes are divided into two ways: a fast search data generation method and a customized information service segmentation method to efficiently search and abbreviate vast amounts of data. In the high-speed search data generation, we discuss the configuration of the synchronization process for the time series analysis of the sensors collected in the underground facility and the additional information method according to the data reduction. In the user-customized service method, we define the types of users in normal and disaster situations, and discuss how to service them accordingly. Through this study, it is expected to be able to develop a systematic data generation and service model for the management of underground utilities that can effectively search and receive large-scale data in a disaster situation.

• Journal of Broadcast Engineering
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• v.27 no.6
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• pp.940-943
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• 2022

This study aims to analyze the Korean dataset by performing Korean sentence Emotion Analysis in the radio listeners' text messages collected personally. Currently, in Korea, research on the Emotion Analysis of Korean sentences is variously continuing. However, it is difficult to expect high accuracy of Emotion Analysis due to the linguistic characteristics of Korean. In addition, a lot of research has been done on Binary Sentiment Analysis that allows positive/negative classification only, but Multi-class Emotion Analysis that is classified into three or more emotions requires more research. In this regard, it is necessary to consider and analyze the Korean dataset to increase the accuracy of Multi-class Emotion Analysis for Korean. In this paper, we analyzed why Korean Emotion Analysis is difficult in the process of conducting Emotion Analysis through surveys and experiments, proposed a method for creating a dataset that can improve accuracy and can be used as a basis for Emotion Analysis of Korean sentences.

• Journal of Broadcast Engineering
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• v.27 no.6
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• pp.885-896
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• 2022

Motor failure in manufacturing plays an important role in future A/S and reliability. Motor failure is detected by measuring sound, current, and vibration. For the data used in this paper, the sound of the car's side mirror motor gear box was used. Motor sound consists of three classes. Sound data is input to the network model through a conversion process through MelSpectrogram. In this paper, various methods were applied, such as data augmentation to improve the performance of classifying fault motors and various methods according to class imbalance were applied resampling, reweighting adjustment, change of loss function and representation learning and classification into two stages. In addition, the curriculum learning method and self-space learning method were compared through a total of five network models such as Bidirectional LSTM Attention, Convolutional Recurrent Neural Network, Multi-Head Attention, Bidirectional Temporal Convolution Network, and Convolution Neural Network, and the optimal configuration was found for motor sound classification.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.74-74
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• 2002

Production of u 1-antitrypsin ($\alpha$AT) in transgenic cows has a great value in the field of medicine. The present study was conducted to determine the effect of chemically defined KSOM media on in vitro development of bovine transgenic nuclear transfer (NT) embryos. An expression plasmid for human $\alpha$AT was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and a human $\alpha$AT target gene into a pcDNA3 plasmid. Cumulus cells as donor nuclei in NT were collected from a Holstein cow and transfected by lipid-mediated method using FuGene6 (Roche Molecular Biochemicals, USA) as reagent. GFP expressed cumulus cells were introduced into recipient oocytes under DIC microscopy equipped with FITC filter set. After electrical fusion and chemical activation, reconstructed embryos were cultured in 1) SOF + 0.8% BSA, 2) KSOM + 0.8% BSA, 3) KSOM + 10% FBS and 4) KSOM +0.01% PVA for 192 h at 39$^{\circ}C$ with 5% $CO_2$, 5% $O_2$ and 90% $N_2$in humidified condition. The development of the embryos was recorded and the GFP expression in blastocyst was determined under FITC filter. The average fusion rate was 73.8% (251/340; n=8). The development rates to 2-4 cells, morula, blastocysts and expression rates in blastocysts varied from 70.3 to 76.5%, 30.2 to 33.8%, 25.4 to 33.8% and 11.8 to 15.6%, respectively. The difference in development and expression rates of embryos among 4 culture groups was not significant (P>0.05). This study indicates that chemically defined KSOM medium is also able to support development of bovine transgenic NT embryos at similar rate of SOF or KSOM supplemented with BSA or serum.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.443-451
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• 2023

Sound Art (Sonic Art) is a branch of contemporary art that has been practiced dominantly in Europe and the Americas since the mid-20th century; and in Korea, it tends to be regarded as a multiple art field or as a subgenre of contemporary music or media art. Since the 2000s, some leading universities in North America and Europe have been opened sound art majors, producing talented people who specialize in this field or work as practical artists, yet it is still considered a non-mainstream art field. It is difficult to find schools that have opened sound arts as their major program in domestic universities. Along with the introduction of a liberal arts curriculum model and teaching methods used in the <Sound Art of Modern Society> course operated in a four-year university in South Korea, this paper discusses the possibility of using sound art as a main subject in liberal arts learning in higher education as a creative fusion liberal arts subject.

• Journal of Broadcast Engineering
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• v.25 no.3
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• pp.405-417
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• 2020

Due to the popularization of the Extended Reality, research is actively underway to implement human motion in real-time 3D animation. In particular, Microsoft developed Kinect cameras for 3D motion information can be obtained without the burden of facilities and with simple operation, real-time animation can be generated by combining with 3D formats such as FBX. Compared to the marker-based motion capture system, however, Kinect has low accuracy due to its lack of estimated performance of joint information. In this paper, two algorithms are proposed to correct joint estimation errors in order to realize natural human motion in motion capture animation system in Kinect camera-based FBX format. First, obtain the position information of a person with a Kinect and create a depth map to correct the wrong joint position value using the human body segment length constraint information, and estimate the new rotation value. Second, the pre-set joint motion range constraint is applied to the existing and estimated rotation value and implemented in FBX to eliminate abnormal behavior. From the experiment, we found improvements in human behavior and compared errors between algorithms to demonstrate the superiority of the system.

• Cartoon and Animation Studies
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• s.49
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• pp.697-712
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• 2017

In recent years, film and animation for cinematic release have embraced stereoscopic vision and the three-dimensional depth it creates for the viewer. The maturation of consumer-level virtual reality (VR) technology simultaneously spurred a wave of media productions set within 3D space, ranging from computer games to pornographic videos, to Academy Award-nominated animated VR short film Pearl. All of these works rely on stereoscopic fusion through stereopsis, that is, the perception of depth produced by the brain from left and right images with the amount of binocular parallax that corresponds to our eyes. They aim to emulate normal human vision. Within more experimental practices however, a fully rendered 3D space might not always be desirable. In my own abstract animation work, I tend to favour 2D flatness and the relative obfuscation of spatial relations it affords, as this underlines the visual abstraction I am pursuing. Not being able to immediately understand what is in front and what is behind can strengthen the desired effects. In 2015, Jeffrey Shaw challenged me to create a stereoscopic work for Animamix Biennale 2015-16, which he co-curated. This prompted me to question how stereoscopy, rather than hyper-defining space within three dimensions, might itself be used to achieve a confusion of spatial perception. And in turn, how abstract and experimental moving image practices can benefit from stereoscopy to open up new visual and narrative opportunities, if used in ways that break with, or go beyond stereoscopic fusion. Noteworthy works which exemplify a range of non-traditional, expanded approaches to binocular vision will be discussed below, followed by a brief introduction of the stereoscopic animation loop III=III which I created for Animamix Biennale. The techniques employed in these works might serve as a toolkit for artists interested in exploring a more experimental, expanded engagement with stereoscopy.

• Trans-
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• v.4
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• pp.1-36
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• 2018

This paper summarizes some of the contents of the 7th International Symposium "Classical Art and Digital" of the Transmedia Institute(TMI), held on October 31, 2017. The purpose of this year's International Symposium was to look back on the genre and the aesthetic history of classical art and to create a place for discourse about the recognition of classical art in the modern digital era. In the first part of the discussion about the duplication of the original and virtual imitation, three presentations on "Visual Image Art and Digital" were introduced. The conclusion of this first discussion is summarized by urging the movement of viewpoint as 'systematic change of the routine aesthetic process'. In the second part on "Performing Arts and Digital", we realize that we need to definitize the 'new directive term system' necessary for the era of digital convergence and performance. In this process we can refer to the need for the emergence of a new aesthetic basis. Two papers in the second part of the paper will introduce the study of 'dance' performance. Some of these studies are reintroduced at the conclusion. The theme of this year's International Symposium can be expected to provide a foothold for the forthcoming second 'Laocoon sculpture argue'. In short, behind the various controversies in the history of aesthetics, this year's conference is concluded with a call for the need to flexibly read the enormous flow of 'art' and 'mental heritage left by art'. It is said that this year's international conferences are presenting the basis of practical and systematic theories for the upcoming fusion media era.