• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.13-22
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• 2012

Plant regeneration has been optimized increasingly by organogenesis and somatic embryogenesis using a range of explants with tissue culture improvements focusing on factors, such as the age of the explant, genotype, media supplements and $Agrobacterium$ co-cultivation. The production of haploids and doubled haploids using microspores has accelerated the production of homozygous lines in Brassicaceae crop plants. Somatic cell fusion has facilitated the development of interspecific and intergeneric hybrids in sexually incompatible species of $Brassica$. Crop improvement using somaclonal variation has also been achieved. Transformation technologies are being exploited routinely to elucidate the gene function and contribute to the development of novel enhanced crops. The $Agrobacterium$-mediated transformation is the most widely used approach for the introduction of transgenes into Brassicaceae, and $in$ $vitro$ regeneration is a key factor in developing an efficient transformation method in plants. Although many other Brassicaceae are used as model species for improving plant regeneration and transformation systems, this paper focuses on the recent technologies used to regenerate the most important Brassicaceae crop plants.

• Proceedings of the Korean Society for Information Management Conference
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• 1997.08a
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• pp.175-181
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• 1997

인쇄매체 중심의 학교도서관에 전자매체, 영상매체, 컴퓨터학습프로그램(CAI: Computer Assist Instruction) 및 첨단 통신장비를 갖추어서 교수 매체센터 체제로 전환하여 정보화 시대의 ‘열린 교육’과 ‘자료중심 교육’에 중추적인 역할을할 수 있도록 모형을 개발하여 전국 각, 시, 도에서 시범운영을 하고자 한다.

• Journal of the Korean Society of Clothing and Textiles
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• v.44 no.6
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• pp.1087-1106
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• 2020

The emergence and development of innovative science and technology is changing lives at a rapid pace with the expansion of human perception. The development of the media extends the scope of activities of the body and the mind, realizing the imagination and challenging the limits of experience. In this process, the transformation of media into new forms such as disassembly, merging, and fusion have emerged that have resulted in the emergence of transmedia. In recent years, the fashion industry has responded quickly to trends and is actively engaged in transmedia communication activities. Among them, Gucci's media utilization strategy has attracted interest in the millennial generation. This study examines the strategy of transmedia storytelling by concentrating on the case of Gucci among luxury fashion brands that utilize media effectively in various aspects. It reveals the aspect of how the use of transmedia can be used to enjoy the story of fashion content and spread its value.

• Journal of the Korean Society for Library and Information Science
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• v.37 no.1
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• pp.101-125
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• 2003

This thesis presents a step-by-step development strategy to implement electronic school libraries corresponding to level 2 among 3 levels of the school library. The implementation of electronic school libraries means the transformation of school libraries into school media centers. This thesis divides the implementation of electronic school libraries into 3 steps. The first step is the implementation of facilities, the second step the implementation of web-based information system, the third step the networking of school libraries, on the basis of the first and second steps. And this thesis presents the basic policy of educational administration aspects to support 3 steps.

• 정보저장시스템학회:학술대회논문집
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• 2005.10a
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• pp.124-127
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• 2005

Rewritable optical memory devices such as an CD-RW and DVD+RW are data storage media, which take advantage of the different optical properties in the amorphous and crystalline states of phase change materials. The switching property, structural transformation, transformation kinetics and chemical bindings of $Ge_xSb_{100-x}$($6{\le}x{\le}$34) were studied to investigate the feasibility of applying $Ge_xSb_{100-x}$ alloys in optical memory. The $Ge_xSb_{100-x}$ thin film was deposited by RF magnetron co-sputtering system and phase change characteristics were investigated by X-ray diffraction (XRD), static tester, inductively coupled plasma atomic emission spectrometer (ICP-AES) and atomic force microscopy (AEM). Optimum fiim composition of $Ge_xSb_{100-x}$ was studied and its minimum time fur laser induced crystallization and optical contrast fur phase transition was performed. These results might be correlated with the binding energies between Ge and Sb, and indicate that $Ge_xSb_{100-x}$ have an potential far optical memory applications.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.11
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• pp.1444-1453
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• 2005

In this study, I tried to analyze the characteristics of modem punk fashion by applying characteristics of modem cultural phenomena. The method of this study is the qualitative study: I analyzed collections from 1996 to 2003 by suggesting an analysis frame based on bibliological studies and applying internet photo materials to the analysis frame. As a result, modem punk fashion has the following characteristics. First, it tends to promote things that are entirely different from the previous expression methods by using the overlapping of texts combined with things that are heterogeneous. Second, the scope of its decoration has been expanded with the aid of the expression method, in which the objects that had not been used as decoration previously have been transformed into media. Third, the appearance of non-mainstream group who has suggested the anti-mainstream thought that violates the uniformed beauty of punk group has expanded the scope of classes that accept the punk fashion. Lastly, clothes, wigs and accessories have been commercialized through the permanent modification that used to signify resistance and disgust such as the expression methods of image replicas using tattoo, piercing, and transformation of hair. In doing so, punk fashion has been generalized and popularized. Such modem punk fashion reflects the change of society such as social transformation that is occupied by images thanks to its complexity and pluralism and the development of public media.

• Smart Media Journal
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• v.10 no.1
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• pp.79-87
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• 2021

In recent years research on automatic font generation with machine learning mainly focus on using transformation-based methods, in comparison, generative model-based methods of font generation have received less attention. Transformation-based methods learn a mapping of the transformations from an existing input to a target. This makes them ambiguous because in some cases a single input reference may correspond to multiple possible outputs. In this work, we focus on font generation using the generative model-based methods which learn the buildup of the characters from noise-to-image. We propose a novel way to train a conditional generative deep neural model so that we can achieve font style control on the generated font images. Our research demonstrates how to generate new font images conditioned on both character class labels and character style labels when using the generative model-based methods. We achieve this by introducing a modified generator network which is given inputs noise, character class, and style, which help us to calculate losses separately for the character class labels and character style labels. We show that adding the character style vector on top of the character class vector separately gives the model rich information about the font and enables us to explicitly specify not only the character class but also the character style that we want the model to generate.

• The Plant Pathology Journal
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• v.35 no.6
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• pp.575-584
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• 2019

Colletotrichum acutatum is a species complex responsible for anthracnose disease in a wide range of host plants. Strain C. acutatum KC05, which was previously isolated from an infected pepper in Gangwon Province of South Korea, was reidentified as C. scovillei using combined sequence analyses of multiple genes. As a prerequisite for understanding the pathogenic development of the pepper anthracnose pathogen, we optimized the transformation system of C. scovillei KC05. Protoplast generation from young hyphae of KC05 was optimal in an enzymatic digestion using a combined treatment of 2% lysing enzyme and 0.8% driselase in 1 M NH₄Cl for 3 h incubation. Prolonged incubation for more than 3 h decreased protoplast yields. Protoplast growth of KC05 was completely inhibited for 4 days on regeneration media containing 200 ㎍/ml hygromycin B, indicating the viability of this antibiotic as a selection marker. To evaluate transformation efficiency, we tested polyethylene glycol-mediated protoplast transformation of KC05 using 19 different loci found throughout 10 (of 27) scaffolds, covering approximately 84.1% of the entire genome. PCR screening showed that the average transformation efficiency was about 17.1% per 100 colonies. Southern blot analyses revealed that at least one transformant per locus had single copy integration of PCR-screened positive transformants. Our results provide valuable information for a functional genomics approach to the pepper anthracnose pathogen C. scovillei.

• Journal of Microbiology and Biotechnology
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• v.22 no.1
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• pp.25-33
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• 2012

The association of verotoxic E. coli and Acinetobacter spp. with various antibiotic-resistant, diarrhogenic, and nosocomial infections has been a cause for concern worldwide. E. coli and A. haemolyticus isolated on a number of selective media were screened for virulence factors, antibiotic resistance, and transformation of resistance genes. Out of 69 E. coli isolates obtained, 25 (35.23%), 14 (20.30%), and 28 (40.58%) were positive for Vtx1&2, Vtx1, and Vtx2, respectively, 49 (71.015%) for extendedspectrum beta-lactamases (ESBLs), 34 (49.28%) for serum resistance, 57 (82.61%) for cell surface hydrophobicity, 48 (69.57%) for gelatinase production, and 37 (53.62%) for hemolysin production. For the 14 A. haemolyticus isolates, only 2 (14.29%) in each case from all the samples investigated were positive for Vtx1, Vtx2 and Vtx1&2 respectively, 8 (57.14%) for ESBLs, 7 (50.00%) for serum resistance, 11 (78.57%) for cell surface hydrophobicity, 4 (28.57%) for gelatinase production, and 8 (57.14%) for hemolysin production. Although transformation occurred among the E. coli and Acinetobacter isolates (transformation frequency: $13.3{\times}10^{-7}-53.4^{-7}$), there was poor curing of the plasmid genes, a confirmation of the presence of stable antibiotic-resistant genes (DNA concentration between 42.7 and 123.8 ${\mu}g$) and intragenetic transfer of multidrug-resistant genes among the isolates. The isolates were potentially virulent and contained potentially transferable antibiotic resistance genes. Detection of virulence factors, antibiotic resistance genes, and transformation among these isolates is a very significant outcome that will influence approaches to proactive preventive and control measures and future investigations. However, continued surveillance for drug resistance among these bacteria and further investigation of the mechanism of action of their virulence factors are a necessity.

• Journal of Information Technology Services
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• v.12 no.4
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• pp.125-143
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• 2013

This paper intends to explore the social and cultural implications of convergence media, based on Goffman's situational approach. The situational segregation of regions makes us to re-interpret the current private and public spheres and helps us to reveal the principle and structure hidden in the new middle region. An environment of convergence media is nullifying the separation of private and public spheres, while letting a new middle region appear.