• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2005.06a
• /
• pp.154-164
• /
• 2005

Saccharomyces cerevisiae KNU5377 is a thermotolerant strain, which can ferment ethanol from wasted papers and starch at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. This strain showed alcohol fermentation ability to convert wasted papers 200 g (w/v) to ethanol 8.4% (v/v) at 40$^{\circ}C$, meaning that 8.4% ethanol is acceptable enough to ferment in the industrial economy. As well, all kinds of starch that are using in the industry were converted into ethanol at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. Hyperthermic cell killing kinetics and differential scanning calorimetry (DSC) revealed that exponentially growing cells of this yeast strain KNU5377 were more thermotolerant than those of S. cerevisiae ATCC24858 used as a control. This intrinsic thermotolernace did not result from the stability of entire cellular components but possibly from that of a particular target. Heat shock induced similar results in whole cell DSC profiles of both strains and the accumulation of trehalose in the cells of both strains, but the trehalose contents in the strain KNU5377 were 2.6 fold higher than that in the control strain. On the contrary to the trehalose level, the neutral trehalase activity in the KNU5377 cells was not changed after the heat shock. This result made a conclusion that though the trehalose may stabilize cellular components, the surplus of trehalose in KNU5377 strain was not essential for stabilization of whole cellular components. A constitutively thermotolerant yeast, S. cerevisiae KNU5377, was compared with a relatively thermosensitive control, S. cerevisiae ATCC24858, by assaying the fluidity and proton ATPase on the plasma membrane. Anisotropic values (r) of both strains were slightly increased by elevating the incubation temperatures from 25$^{\circ}C$ to 37$^{\circ}C$ when they were aerobically cultured for 12 hours in the YPD media, implying the membrane fluidity was decreased. While the temperature was elevated up to 40$^{\circ}C$, the fluidity was not changed in the KNU5377 cell, but rather increased in the control. This result implies that the plasma membrane of the KNU5377 cell can be characterized into the more stabilized state than control. Besides, heat shock decreased the fluidity in the control strain, but not in the KNU5377 strain. This means also there's a stabilization of the plasma membrane in the KNU5377 cell. Furthermore, the proton ATPase assay indicated the KNU5377 cell kept a relatively more stabilized glucose metabolism at high temperature than the control cell. Therefore, the results were concluded that the stabilization of plasma membrane and growth at high temperature for the KNU5377 cell. Genome wide transcription analysis showed that the heat shock responses were very complex and combinatory in the KNU5377 cell. Induced by the heat shock, a number of genes were related with the ubiquitin mediated proteolysis, metallothionein (prevent ROS production from copper), hsp27 (88-fold induced remarkably, preventing the protein aggregation and denaturation), oxidative stress response (to remove the hydrogen peroxide), and etc.

• Cartoon and Animation Studies
• /
• s.43
• /
• pp.47-75
• /
• 2016

This study has analyzed the history of the subcontract animation in Korea that began with Golden Bat of TBC Animation Division in 1966 to 1980s and shed the light on the history of subcontract animation that has been processed over 30 years in Korean animation. For this purpose, through the outlined status of subcontract animation, such as, production company, production status, scale of industry and so forth, the status of the OEM industry then has been checked and it links the solidified background of animation into subcontract production industry with the situation in time for analysis. In addition, on the basis of the foregoing, it is intended to broaden the horizon of the history of animation through the analysis on new search for facilitating the creative animation by overcoming the issues and limits generated by the subcontract animation industry. 1970s was the time that the national objective is to advance heavy-chemical industry and export-led economic growth. From the late 1970s, the animation has been spot lighted as the main-stream export industry through the overseas subcontract orders for animation. Expansion of the subcontract animation production has been influenced from the national policies on public culture, dispersion of color TV, facilitation of video production market and other media changes of the time that led the decline of animation audiences in theaters, and another cause would be in lack of platform of broadcasting companies that avoided the independent animation production for its economic theory. The subcontract animation industry may have the positive evaluation in the aspect of expanding the animation environment, such as, structuring of animation infra, development of new human resources and etc. However, the technology-incentive 'production'-oriented advancement has created distorted structure in advancing the professional human resources due to the absence of 'pre-production' of planning and others as well as the insufficient perception on 'post production (post work)', and it was unable to formulate domestic market by re-investing the capital accumulated for OEM industry into the production of creative animation and it has been assessed as negative aspect. Animation is a cultural and spiritual product of a country. Therefore, the systematic support policy for the facilitation of the creative animation, such as, development of professional human resources, creation of outstanding work, formation of market to make the pre-circulation structure and so forth has to be sought. However, animation is an industry, but there is no perception that it is a cultural industry based on the creativeness, not hardware-oriented manufacturing business. Such a lack of recognition, there was no policies to make the market and facilitate the creative animation by the animation of Korea for this period through the long-term plan and investment for independent work production. Such an attempt is newly begun through diverse searches for protection and advancement of creative animation in Korea after 1990s.

• Cartoon and Animation Studies
• /
• s.48
• /
• pp.359-383
• /
• 2017

3,000 years ago, since the first poster of humanity appeared in Egypt, the invention of printing technique and the development of civilization have accelerated the poster production technology. In keeping with this, the expression of poster has also been developed as an attempt to express artistic sensibility in a simple arrangement of characters, and now it has become an art form that has become a domain of professional designers. However, the technological development in the expression of poster is keep staying in two-dimensional, and is dependent on printing only that it is irrelevant to the change of ICT environment based on modern multimedia. Especially, among the many kinds of posters, the style of movie posters, which are the only objects for video, are still printed on paper, and many attempts have been made so far, but the movie industry still does not consider ICT integration at all. This study started with the feature that the object of the movie poster dealt with the video and attempted to introduce the augmented reality to apply the dynamic image of the movie to the static poster. In the graduation work of the media design major of a university in Korea, the poster of each works for promoting the visual work of the students was designed and printed in the form of a commercial film poster. Among them, 6 artworks that are considered to be suitable for augmented reality were selected and augmented reality was introduced and exhibited. Content that appears matched to the poster through the mobile device is reproduced on a poster of a scene of the video, but the text informations of the original poster are kept as they are, so that is able to build a moving poster looked like a wanted from the movie "Harry Potter". In order to produce this augmented reality poster, we applied augmented reality to posters of existing commercial films produced in two different formats, and found a way to increase the characteristics of AR contents. Through this, we were able to understand poster design suitable for AR representation, and technical expression for stable operation of augmented reality can be summarized in the matching process of augmented reality contents production.

• Journal of radiological science and technology
• /
• v.28 no.4
• /
• pp.279-286
• /
• 2005

Purpose : There are two modalities, those are small bowel series(SBS) and abdominal pelvic computed tomography(CT), for diagnosis of small bowel disease. The aim of this study is to lend radiological technologists who are doing the two modalities assistance in the understanding characteristic of disease by comparing the two results. Meterials and method : 284 patients were examined the two SBS and abdominal pelvic CT together from 1999 to 2003. 250 ml $BaSO_4$ suspension 40 w/v% and 600ml carboxy methyl cellulose 0.5 w/v% were used for SBS. Abdominal Pelvic CT was examined in one hour before taking 450 ml $BaSO_4$ suspension 1.5 w/v%. The CT scan was done in 72 sec after 150 ml contrast media injection. the used protocol was helical mode 5:5 mm pitch 1.375:1, speed 27.50, exposure 120 kv, 240 mA, tube rotation time 0.5 sec. the statistic analysis was conducted with statistical program SPSS 10 version with frequency and crossing analysis. P-value less than 0.05 were considered significant. Results : In the results of SBS, normal findings were 131 patients(46.1%), inflammatory bowel disease(IBD) 64(22.9%), ischemia+ileocolitis+vasculitis 22(7.7%), Obstruction+stricture 21(7.7%) and Others 45(15.9%). In the results of abdominal pelvic CT, normal findings were 103 patients(36.3%), inflammatory bowel disease 65(22.9%), wall thickening+lymphadenopathy 42(14.8%), Fluid collection 17(6%), and Others 57case(20%). The same results of the two were 130patients(45.8%). 30patients(10.6%) of normal finding in SBS were diagnosed as wall thickening+lymphadenopathy and IBD in CT, and 15patients(5.3%) of normal finding in CT were diagnosed as ischemia+ileocolitis+vasculitis, mass and IBD in SBS(p<0.05). Transit time delay was diagnosed in 10patients(3.5%) on only SBS, wall thickening+lymphadenopathy was diagnosed in 20patients(7%) in only CT(p<0.05). Conclusion : We think that proper examination method will be selected in the small bowel disease, if we understand the characteristics of the disease and method.

• Journal of radiological science and technology
• /
• v.32 no.3
• /
• pp.325-334
• /
• 2009

Purpose : This study was conducted to assess how effective the permeability ratio and relative cerebral blood volume ratio are to tumor through perfusion MRI by measuring and reflecting the grade assessment and differential diagnosis and the permeability and relative cerebral blood volume of contrast media plunged from blood vessel into organ due to breakdown of blood-brain barrier in cerebral. Subject and Method : Subject of study was 29 patients whose diagnosis were confirmed by biopsy after surgery and 550 (11 slice$\times$50 image) perfusion MRI were used to make image of relative cerebral blood volume with the program furnished on instrument. The other method was to transmit to private computer and the image analysis was made additionally by making image of relative cerebral blood volume-reformulated singular value decomposition, rCBV-rSVD and permeability using IDL.6.2. In addition, Kruskal-wallis test tonggyein non numerical average by a comparative analysis of brain tumors Results : The rCBV ratio (Functool PF; GE Medical Systems and IDL 6.2 program by analysis) and permeability ratio of tumors were as follows; high grade glioma(n=4), (14.75, 19.25) 13.13. low grade astrocytoma(n=5) (14.80, 15.90) 11.60, glioblastoma(n=5) (10.90, 18.60), 22.00, metastasis(n=6) (11.00, 15.08). 22.33. meningioma(n=6) (18.58, 7.67), 5.58. oliogodendroglioma(n=3) (23.33, 16.33, 15.67. Conclusion : It was not easy to classify the grade with the relative cerebral blood volume ratio measured by using the relative cerebral blood image by type of tumors, however, permeability ratio measured by permeability image revealed that the higher the grade of tumor, the higher the measured permeability ratio, showing the assessment of tumor grade is more effective to differential diagnosis.

• Horticultural Science & Technology
• /
• v.18 no.6
• /
• pp.834-838
• /
• 2000

As an endeavor to establish a micropropagation system for Epimedium koreanum Nakai., this study was carried out to define methods to disinfect its explants and media for callus induction, proliferation and plant regeneration. The lowest infection rates by fungi or bacteria on apical and axillary bud explants of rhizome were observed when they were immerged in 0.3% NaOCl solution for 20 min after soaked in 0.1% $AgNO_3$ solution for 30 min, but leaf explants were seldom infected with fungi or bacteria by this disinfectant method. The highest rate of plantlet formation was obtained from the explants disinfected in 0.3% NaOCl solution for 20 min after soaked in 0.1% $AgNO_3$ solution for 60 min for tip buds and in 0.1 % $AgNO_3$ solution for 30 min for axillary buds of rhizome. Induction rate of callus was the highest from the explants disinfectd in 0.3% NaOCl solution for 20 min after soaked in 0.2% $AgNO_3$ solution for 15 min. Callus growth was proper in a modified 1/2 MS medium including half strength of $NH_4NO_3$ with $0.02-0.2mg{\cdot}L^{-1}$ BA and $2.0mg{\cdot}L^{-1}$ NAA. Low rate of plantlet regeneration was obtained in 1/2 UM or 1/2 White medium with $2.0mg{\cdot}L^{-1}$ BA and $0.2mg{\cdot}L^{-1}$ AA.

• Applied Microscopy
• /
• v.38 no.4
• /
• pp.279-284
• /
• 2008

The phase change materials have been extensively used as an optical rewritable data storage media utilizing their phase change properties. Recently, the phase change materials have been spotlighted for the application of non-volatile memory device, such as the phase change random access memory. In this work, we have investigated the crystallization behavior and microstructure analysis of In-Sb-Te (IST) thin films deposited by RF magnetron sputtering. Transmission electron microscopy measurement was carried out after the annealing at $300^{\circ}C$, $350^{\circ}C$, $400^{\circ}C$ and $450^{\circ}C$ for 5 min. It was observed that InSb phases change into $In_3SbTe_2$ phases and InTe phases as the temperature increases. It was found that the thickness of thin films was decreased and the grain size was increased by the bright field transmission electron microscopy (BF TEM) images and the selected area electron diffraction (SAED) patterns. In a high resolution transmission electron microscopy (HRTEM) study, it shows that $350^{\circ}C$-annealed InSb phases have {111} facet because the surface energy of a {111} close-packed plane is the lowest in FCC crystals. When the film was heated up to $400^{\circ}C$, $In_3SbTe_2$ grains have coherent micro-twins with {111} mirror plane, and they are healed annealing at $450^{\circ}C$. From the HRTEM, InTe phase separation was occurred in this stage. It can be found that $In_3SbTe_2$ forms in the crystallization process as composition of the film near stoichiometric composition, while InTe phase separation may take place as the composition deviates from $In_3SbTe_2$.

• Horticultural Science & Technology
• /
• v.29 no.2
• /
• pp.87-94
• /
• 2011

This study was carried out to investigate the effects of hot solution soaking and chilling treatment on sprouting and enlargement of bulblets obtained through in vitro culture in Korean native lilies with ornamental values. In vitro bulblets of Lilium cernuum, L. hansonii, L. hansonii for. mutatum, L. leichtlinii, and L. tsingtauense were soaked in distilled water or 100 $mg{\cdot}L^{-1}$ $GA_3$ and $GA_{4+7}$ solution maintained at $35^{\circ}C$ for two hours (hot water treatment) and/or exposed at $4^{\circ}C$ for 0, 4, or 8 weeks (chilling treatment) and then planted in plastic trays filled with media and grown in a greenhouse at $20^{\circ}C$ and under 16 h photoperiod. In all species, no bulblet propagated by tissue culture sprouted without chilling or hot water treatment due to dormancy. For dormancy breaking, $GA_{4+7}$ hot solution treatment increasing sprouting by 55-96%, whereas distilled water or GA was not effective in sprouting. Chilling treatment for 4 weeks induced sprouting by 50-70% in L. cernuum and L. leichtlinii, whereas 8 weeks was needed for sprouting of L. hansonii and L. hansonii for. mutatum. Combined treatment of hot water and chilling treatments synergistically promoted sprouting. Especially, in L. cernuum and L. hansonii, $GA_{4+7}$ hot solution soaking prior to chilling for 4 weeks promoted sprouting by 35-45% compared with the reverse order. Enlargement of bulblets resulted from increase in fresh weight and diameter was promoted by the treatments that increased the sprouting percentage of bulblets. Only in L. cernuum, shoots emerged from bulblets soaked in hot $GA_{4+7}$ solution or chilled at $4^{\circ}C$ and shoot emergence rate was highest in bulblets soaked in hot $GA_{4+7}$ solution and then chilled for 8 weeks. From these results, the most effective method for bulblet sprouting and enlargement was to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. hansonii, hansonii for. mutatum, and leichtlinii, and to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. cernuum and tsingtauense.

• Journal of Internet Computing and Services
• /
• v.21 no.5
• /
• pp.139-148
• /
• 2020

Infectious diseases have long plagued mankind, and predicting and preventing them has been a big challenge for mankind. For this reasen, various studies have been conducted so far to predict infectious diseases. Most of the early studies relied on epidemiological data from the Centers for Disease Control and Prevention (CDC), and the problem was that the data provided by the CDC was updated only once a week, making it difficult to predict the number of real-time disease outbreaks. However, with the emergence of various Internet media due to the recent development of IT technology, studies have been conducted to predict the occurrence of infectious diseases through web data, and most of the studies we have researched have been using single Web data to predict diseases. However, disease forecasting through a single Web data has the disadvantage of having difficulty collecting large amounts of learning data and making accurate predictions through models for recent outbreaks such as "COVID-19". Thus, we would like to demonstrate through experiments that models that use multiple Web data to predict the occurrence of infectious diseases through LSTM models are more accurate than those that use single Web data and suggest models suitable for predicting infectious diseases. In this experiment, we predicted the occurrence of "Malaria" and "Epidemic-parotitis" using a single web data model and the model we propose. A total of 104 weeks of NEWS, SNS, and search query data were collected, of which 75 weeks were used as learning data and 29 weeks were used as verification data. In the experiment we predicted verification data using our proposed model and single web data, Pearson correlation coefficient for the predicted results of our proposed model showed the highest similarity at 0.94, 0.86, and RMSE was also the lowest at 0.19, 0.07.

• Journal of dental hygiene science
• /
• v.8 no.4
• /
• pp.313-322
• /
• 2008

The purpose of this study was to examine the awareness of dental hygienists in different regions about credit bank system and their needs for that. The subjects in this study were 312 dental hygienists in dental hospitals and clinics in Seoul, Gyeonggi province, metropolitan cities, Gangweon province and Gyeongsang province. After a survey was conducted in 2008, the collected data were analyzed with SPSS 11.5 program. The findings of the study were as follows: 1. In regard to perception of the credit bank system, 68.3 percent of the dental hygienists were cognizant of that. The dental hygienists from most of the geographic areas were aware of it, but all the forty three dental hygienists from Gangweon province who accounted for 13.8 percent didn't know about that. As for media by which they acquired information on that, many of the dental hygienists learned about it from their acquaintances (46.2%), but that was not the case for the dental hygienists from Seoul and Gyeonggi province. 2. As for expectations for the credit bank system, those from Gyeongsang province had the greatest expectations for that in six out of nine items, which included academic or job performance improvement, acquisition of credits, degree or certificates and access to new technology. 3. Concerning educational spending on the credit bank system, 18.9 percent and 8.7 percent from Seoul/Gyeonggi province and Gangweon province spent less than 30 thousand won, and 19.9 percent from the metropolitan cities spent 30 thousand to less than 50 thousand won. 4. As for the importance of the categories of their major in the credit bank system, those from Gangweon province attached the most importance to oral health education ($3.56{\pm}1.259$), and the dental hygienists from the metropolitan cities put the most stress on preventive treatment ($3.64{\pm}1.191$).