• Journal of Embryo Transfer
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• v.19 no.3
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• pp.275-282
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• 2004

This study was carried out to examine the effects of development media and those change on in vitro development (IVD) of porcine oocytes matured and fertilized in vitro. Putative embryos, which were matured in vitro in modified NCSU-23 (mNCSU-23) supplemented with 10％ porcine follicular fluid (pFF) and were fertilized in mTBM, were developed in vitro as the experimental scheme. The results are as follows. When porcine putative embryos were cultured in vitro in NCSU-23 or CZB/Pig-MEM, the percentage of oocytes cleaved was not different between two systems, but the percentage of blastocysts in NCSU-23 was significantly higher than in CZB/Pig-MEM (P<0.05). And when porcine putative embryos were cultured in vitro in NCSU-23 during 7 days with or without changing media at day 5, which was supplement with or without 10％ fetal bovine serum(FBS), the percentage of oocytes cleaved, blastocysts at day 6, and the cell number of ICM, TE and total of blastocysts at day 7 were not different among three treatments. As a result of this study, it is supposed that NCSU-23 be more favorable, to develop porcine embryos derived from IVM/IVF, than CZB/Pig-MEM, but that demonstration on the effects of changing medium with fresh one stand in need of the more experiments.

• The Journal of the Korea Contents Association
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• v.22 no.5
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• pp.56-66
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• 2022

Due to the recent increase in information encountered on social media, algorithm-based recommendation formats selectively provide information based on user information, which often causes a filter bubble effect by an Echo Chamber. Eco-chamber refers to a phenomenon in which beliefs are amplified or strengthened by communication only in an enclosed system, and filter bubbles refer to a phenomenon in which information providers provide customized information according to users' interests, and users encounter only filtered information. The purpose of this study is to propose a method of efficiently selecting information as a way to improve the filter bubble phenomenon by such an echo chamber. The research progress method analyzed recommended algorithms used on YouTube, Facebook and Amazon. In this study, humanities solutions such as training critical thinking skills of social media users and strengthening objective ethical standards according to self-preservation laws, and technical solutions of model-based cooperative filtering or cross-recommendation methods were presented. As a result, recommended algorithms should continue to supplement technology and develop new techniques, and humanities should make efforts to overcome cognitive dissonance and prevent users from falling into confirmation bias through critical thinking training and political communication education.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.544-547
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• 1999

The object of this study was to determine the influence of monensin and virginiamycin (VM) on in vitro ruminal fermentation of rice straw or ammoniated rice straw. Rumen fluid was collected from 4 wethers fed 200 g of concentrate supplement with 400 g of untreated (U) or ammoniated (A) rice straw once daily for 28 days. Mixed ruminal microorganisms were incubated in anaerobic media that contained 20% (vol/vol) ruminal fluid and 0.3 g of either U or A rice straw. Monensin and/or VM, dissolved in ethanol, were added in centrifuge tubes at final concentrations of 0, 15, 30, 15+15 and 30+30 ppm of culture fluid. The addition of monensin and VM combination to A rice straw fermentation decreased (p<0.05) the acetate to propionate ratio, total VFA and lactate production, but increased (p<0.05) pH. Total gas production tended to be decreased by the addition of monensin plus VM. Antimicrobial agents decreased $NH_3$ N concentration and dry matter digestibility.

• Korean Institute of Interior Design Journal
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• v.27 no.1
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• pp.12-20
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• 2018

After the "Landscaping Law" was enacted in 2007 Korea, national and local governments has been responding swiftly by seeking various methods. Seoul Metropolitan City and other municipalities have established "Seoul Coloring and Systematization" project, which analyzes and extracts the humanities, humanities and natural environments in 2007 as an opportunity to recognize the importance of environmental color. However, it is necessary to slightly supplement the color scheme created by the observer's concept. In this way, you have to find the color of the environment in a city where real life takes place. The city's environmental color has the concept of "walking" as the basic human behavior. Because the average speed is 4km, it differs from that of a vehicle that travels more than 60km per hour. Also, most of the frontage zone is used except for special cases. Therefore the characteristics of the environmental color and frontage zone on the road side were concluded in this paper as Single Type, Connection Type, and Extension Type. In addition, the principle of environmental color of city was established by the change of texture according to space of time and the characteristics of urban scale, and new media characteristics were found in various interactions. The results of this study suggest an environmental coloring strategy for workability such as hue continuity, hue harmonization and integrated design.

• Journal of Broadcast Engineering
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• v.14 no.4
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• pp.419-427
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• 2009

We present a novel head tracking system for stereoscopic displays that ensures the viewer has a high degree of movement. The tracker is capable of segmenting the viewer from background objects using their relative distance. A depth camera using TOF(Time-Of-Flight) is used to generate a key signal for eye tracking application. A method of the moving parallax barrier is also introduced to supplement a disadvantage of the fixed parallax barrier that provides observation at the specific locations.

• Journal of Korea Multimedia Society
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• v.22 no.11
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• pp.1313-1323
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• 2019

With the development of the Internet and the increase of smart phones, various services considering user convenience are increasing, so that users can check news in real time anytime and anywhere. However, online news is categorized by media and category, and it provides only a few related search terms, making it difficult to find related news related to keywords. In order to solve this problem, we propose a method to recommend related documents more accurately by applying Doc2Vec similarity to the specific keywords of news articles and weighting the title and contents of news articles. We collect news articles from Naver politics category by web crawling in Java environment, preprocess them, extract topics using LDA modeling, and find similarities using Doc2Vec. To supplement Doc2Vec, we apply TF-IDF to obtain TC(Title Contents) weights for the title and contents of news articles. Then we combine Doc2Vec similarity and TC weight to generate TC weight-similarity and evaluate the similarity between words using PMI technique to confirm the keyword association.

• Journal of the Korean Applied Science and Technology
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• v.28 no.2
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• pp.232-239
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• 2011

Fabric fibrous filter has been used in various industrial applications owing to the low cost and wide generality. However, the basic properties of fabric materials often limit the practical utilization including hot gas cleaning. This study attempts to find new coatings of porous fibrous filter media in order to overcome its insufficient thermal resistance and durability. Teflon was one of the plausible chemicals to supplement the vulnerability against frequent external thermal impacts. A foaming agent composed of Teflon and some organic additives was tentatively coated on the glass fiber mat. The present test Teflon foam coated filter was fount to be useful for hot gas cleaning, up to $250^{\circ}C$-$300^{\circ}C$. Close examination using XPS(X-ray Photoelectron Spectroscopy) and Contact angle proved the binding interactions between carbon and fluorine, which implies coating stability. The PTFE/Glass foam coated filter consisted of more than 95% (C-F)n bond, and showed super-hydrophobic with good-oleophobic characteristics. The contact angle of liquid droplets on the filter surface enabled to find the filter wet-ability against liquid water or oil.

• Nuclear Engineering and Technology
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• v.51 no.2
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• pp.626-630
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• 2019

After several significant nuclear accidents, public attitudes toward nuclear energy technologies and facilities are considered to be one of the essential factors in the national energy and electricity policy-making process of several nations that employ nuclear energy as their key energy resource. However, it is difficult to explore and capture such an attitude, because the majority of prior studies analyzed public attitudes with a limited number of respondents and fragmentary opinion polls. In order to supplement this point, this study suggests a big data analyzing method with K-LIWC (Korean-Linguistic Inquiry and Word Count), sentiment and query analysis methods, and investigates public attitudes, positive and negative emotional statements about nuclear energy with the collected data sets of well-known social media and network services in Korea over time. Results show that several events and accidents related to nuclear energy have consistent or temporary effects on the attitude and ratios of the statements, depending on the kind of events and accidents. The presented methodology and the use of big data in relation to the energy industry is suggested as it can be helpful in addressing and exploring public attitudes. Based on the results, implications, limitations, and future research areas are presented.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.123-123
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• 2003

This study was conducted to determine the ability of nuclear development of canine oocytes depend on the kind of maturation media and addition of serum sources. Ovaries were collected from a bitches at various stages of estrus cycle by an ovariohysterectomy. Oocytes were collected of cumulus oocytes complexes after slicing of ovaries with blade. The maturation medium was containing 0.6 mM/ml cysteine, 0.2 mM pyruvic acid, 20 ng/ml $E_2$ and 1 $\mu g/ml$ rbST Exp. 1, the oocytes were matured in four different maturation medium as follows: 1) TCM-199, 2) DMEM, 3) NCSU37 and 4) modified-NCSU37 with 10% FBS. Exp. 2: the oocytes were matured in mNCSU37 supplemented with different protein sources (10% FBS, 10% EDS, 0.3% BSA and 0.1% PVA) to select the optimal one. Oocytes were matured in a humidified atmosphere containing 5% $CO_2$ at $39{\circ}C$ for 72 hrs. The maturation rate were analyzed by Duncan's multiple range test using General Linear Models procedure in SAS. The rates of meiotic resumption to MI-MII depend on different culture media were achieved with TCM-199 (5.2%), DMEM (5.0%), NCSU37 (7.2%) and m-NCSU37 (5.9%), respectively. The rates of meiotic resumption to MI-MII according to addition of protein source were 10% FBS (13.3%), 10% EDS (25.0%), 0.3% BSA (25.0%) and 0.1% PVA (15.4%), respectively. In conclusion, the results obtained showed that in vitro maturation media and protein supplement to m-NCSU37 culture medium tested did not promote the final steps of IVM in canine oocytes.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.135-141
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• 2010

Embryoid bodies (EBs) generated from human embryonic stem cells (hESCs) include spontaneously induced endodermal lineage cells (ELCs). Activin-A plays important roles in the endoderm differentiation of hESCs. Despite studies on the generation of ELCs from hESCs with treatment of Actvin-A, it was unclear for localization and pattern of ELCs by Activin-A during differentiation of hESCs. Accordingly in this study, we knew that Actvin-A increased the cystic EBs formation, including the highly enriched AFP (endoderm lineage specific marker)-expressing cells in the surface of cystic EBs. To induce the EBs formation from undifferentiated hESCs, cells were transferred onto petri-dish and cultured in suspension condition without bFGF removed hESC media (EB media) for 3 days. Next to investigate the effect of Activin-A, EBs were subsequently cultured in EB media supplement with 100 ng/ml Activin-A for 3 days. After 5~7 days of Activin-A treatment, cystic EBs began to appear which increased in numbers reaching ~60% of initially formed EBs over 5 days. Endoderm lineage marker, AFP were highly expressed and specifically localized at the surface region of cystic EBs comparison with normal EBs. We next attached the cystic EBs onto gelatin-coated plates and cultured for 5 days. In the results of real-time PCR and immunocytochemistry analysis, AFP-expressing cells migrated and localized at the outgrowth region of attached cystic EBs. To obtain the AFP-expressing cells of the outgrowth region, we manually isolated by using micro-dissection and cultured them. These cells strongly express AFP over 70% of isolated cells post re-plating. Here, we first showed an expression pattern of specifically localized ELCs by Activin-A during differentiation of hESCs. From this observation, we could highly purified ELCs from undifferentiated hESCs. Taken together, our system will provide a novel and efficient option to generate ELCs from hESCs.