• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.7-13
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• 2004

We have investigated the optimal conditions for the growth and ginsenoside production in adventitious root of “Chunpoong” (Panax ginseng C.A. Meyer). Ginseng adventitous roots induced from the embryo of “Chunpoong” were cultured in various plant media supplemented with several growth hormones. The best growth rate and high ginsenoside contents were obtained in B5 medium supplemented with IBA (2 mg/L) and kinetin (0.1 mg/L). The supplement of 2.5 mM KH$_2$PO$_4$ was good for high growth rate of the adventitious roots, but the accumulation of ginsenosides was increased by reducing the KH$_2$PO$_4$ concentration to 1.25 mM. We have established the effective liquid culture system for the optimal growth and ginsenoside production of “Chunpoong” adventitious roots.

• Journal of National Security and Military Science
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• s.15
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• pp.1-30
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• 2018

This study focused on how to supplement and develop military spiritual education in response to promotion of peace and prosperity and Change of Security Environment on the Korean Peninsula. In order to succeed in the peace and prosperity on the Korean Peninsula policy, Strengthening the military spiritual education is necessary. This study was analyzed through the survey and the results are as follows: First, the awareness level of military spiritual education is more than 68%. Second, the satisfaction level of education has been quit motivated, but, the respondents demands various methods of eduction. Third, the current mental power scores showed no significant difference for environmental change. In addition, based on the results of the research, the problem of military spiritual education is as follows. It is not enough to secure the identity that can firmly support the era of peace and prosperity on the Korean peninsula. Poor budget support might hinder improving poor education facilities and outdated equipment. Furthermore, there is a lack of research on future-oriented educational system in support of traditional education methods of repetition-type repeated education and unification. Therefore, we deducted the following development strategies for the military spiritual education in this paper. First, it is necessary to strengthen the military spiritual education to support the era of peace and prosperity on the Korean Peninsula. Second, for enhancing educational environments, the educational facilities and equipments should be improved by understanding the characteristics of the education target. Third, the integrated management of military research institutes specialized in military spiritual education should be pursued as a system development for ensuring the continuous effect of education. In conclusion, continuous attention and research are needed to establish national perspective and national security perspective, raise the military spirit and utilize various education development programs in order to develop efficient military spiritual education in the future.

• Journal of Broadcast Engineering
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• v.24 no.3
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• pp.515-526
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• 2019

In the real world, the user's preference for a particular product is determined by many factors besides the quality of the product. The reflection of these external factors was very difficult because of various fundamental problems including lack of data. However, access to external factors has become easier as the infrastructure for public data is opened and the availability of evaluation platforms with diverse and vast amounts of data. In accordance with these changes, this paper proposes a recommendation system structure that can reflect the collectable factors that affect user's preference, and we try to observe the influence of actual influencing factors on preference by applying case. The structure of the proposed system can be divided into a process of selecting and extracting influencing factors, a process of supplementing insufficient data using sentence analysis, and finally a process of combining and merging user's evaluation data and influencing factors. We also propose a validation process that can determine the appropriateness of the setting of the structural variables such as the selection of the influence factors through comparison between the result group of the proposed system and the actual user preference group.

• Journal of The Korean Society of Integrative Medicine
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• v.10 no.2
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• pp.61-72
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• 2022

Purpose : This study aimed to identify the educational effects of the International Development Cooperation and Healthcare course from 2018 to 2020. Methods : Changes in awareness of international development cooperation (understanding international development cooperation as well as government Official Development Assistance [ODA], thoughts on aid, and thoughts on government's aid scale) were analyzed using web-based surveys. The pre-course survey was conducted 1-2 weeks before the semester started, and the post-course survey was conducted 1-2 weeks after the semester ended. All data were analyzed using the SPSS 26.0 program, and statistical significance was set at p< .05. Results : A total of 314 people participated in the survey before taking the course, and 286 people participated in the survey after taking the course. Analysis of the changes in students' awareness of international development cooperation showed that all items (understanding international development cooperation and government ODA, as well as having thoughts on aid and government's aid scale) were improved. Regarding changes in awareness according to general characteristics, thoughts on aid were statistically significant according to all characteristics except for 2018. Regarding government's aid scale, awareness scores increased after taking the course in students who majored in healthcare, fine arts and athletics, broadcasting and media studies, and digital contents. Conclusion : This study showed that changes in awareness of international development cooperation were largest in second-year students. Compared to changes regarding international development cooperation, government ODA, and thoughts on aid, changes regarding thoughts on government's aid scale were minor. In the future, it is necessary to develop class content suitable for the first-year level and to supplement and operate content that can change the way of thinking on government's aid scale.

• Journal of Ginseng Research
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• v.46 no.3
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• pp.408-417
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• 2022

Background: Korean Red Ginseng extract (KRGE) has been used as a health supplement and herbal medicine. Astrocytes are one of the key cells in the central nervous system (CNS) and have bioenergetic potential as they stimulate mitochondrial biogenesis. They play a critical role in connecting the brain vasculature and nerves in the CNS. Methods: Brain samples from KRGE-administered mice were tested using immunohistochemistry. Treatment of human brain astrocytes with KRGE was subjected to assays such as proliferation, cytotoxicity, Mitotracker, ATP production, and O₂ consumption rate as well as western blotting to demonstrate the expression of proteins related to mitochondria functions. The expression of hypoxia-inducible factor-1α (HIF-1α) was diminished utilizing siRNA transfection. Results: Brain samples from KRGE-administered mice harbored an increased number of GFAP-expressing astrocytes. KRGE triggered the proliferation of astrocytes in vitro. Enhanced mitochondrial biogenesis induced by KRGE was detected using Mitotracker staining, ATP production, and O₂ consumption rate assays. The expression of proteins related to mitochondrial electron transport was increased in KRGE-treated astrocytes. These effects were blocked by HIF-1α knockdown. The factors secreted from KRGE-treated astrocytes were determined, revealing the expression of various cytokines and growth factors, especially those related to angiogenesis and neurogenesis. KRGE-treated astrocyte conditioned media enhanced the differentiation of adult neural stem cells into mature neurons, increasing the migration of endothelial cells, and these effects were reduced in the background of HIF-1α knockdown. Conclusion: Our findings suggest that KRGE exhibits prophylactic potential by stimulating astrocyte mitochondrial biogenesis through HIF-1α, resulting in improved neurovascular function.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.23 no.2
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• pp.1-6
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• 2023

The purpose of this study is to research and develop so that users who are gradually progressing in the popularization of smartphones and the calculation of agricultural quality can use more active and flexible applications than existing application fields. People use event management applications to remember what they need to do, and maps applications to get to their appointments on time. You will need to build a glue-delivered application that leverages the Maps API to be able to recommend the glove's path for events so that the user can use the application temporarily. By comparing and analyzing currently used calendar, map, and schedule applications, several Open Maps APIs were compared to supplement the weaknesses and develop applications that converge the strengths. The results of application development by applying the optimal algorithm for recommending traffic routes according to time and place for the schedule registered by the user are described.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.89-89
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• 1995

Surface epithelial cells isolated from hamster tracheas and grown on a thick collagen gel become a highly enriched population of mucus-secreting cells. Epithelial cells from tracheas of hamsters were collected using enzymatic procedures and cultured under various conditions. The medium used consisted of a 1:1 mixture of medium 199 and Dulbecco's modified Eagle's (DME) medium which was conditioned before use. Insulin, transferrin, hydrocortisone, epidermal growth factor, and extract from bovine hypothalamus were used as supplement. Due to relatively low basal rates of min secretion from in vitro cultures, cultures are generally radiolabeled using $^3$H-glucosamine as a metabolic precursor. The radiolabeled mucinsreleased are quantitated by precipitation with TCA/PTA. Using this cell culture system, we investigated mucin release of goblet cells by altering the media bathing the apical surface of hamster tracheal surface epithelial(HTSE) cells. Acidic media added sulfuric acid caused sigcificant increases in mucin relesse (155${\pm}$20% at pH 4 and 146${\pm}$16% at, pH 5). Ammonium hydroxide also increased mucin release at pH 9.0(156${\pm}$17%) and pH 10(295${\pm}$9%) respectively. This additional mucin release seems to be associated with cell membrane damage as indicated by release of cellular LDH. SP stimulates secretion of mucin in cultured HTSE cells(154${\pm}$16% at 1${\times}$10$\^$-6/M and 165${\pm}$25% at 1${\times}$10$\^$-5/M. PAF at 5${\times}$10$\^$-6/M and 5${\times}$10$\^$-5/M enhanced by HTSE cells in vitro 168${\pm}$34% and 259${\pm}$30% of mucin secretion, respectively. The increase in mucin release by PAF and SP was not secondary to cell damage or necrosis. SP and PAF may be in mediating mucous secretion induced by inflammation irritantion and infection.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.93-100
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• 2009

This study was carried out to investigate the efficient in vitro mass propagation methods for juvenile sporophytes of Matteuccia struthiopteris. Chopped segments of pinnae, petiole and rhizome were cultured on 1/2MS with 0.1% activated charcoal. Among these explant sources only rhizome segments produced young sporophytes, regenerating vigorously on 1/2 MS medium. Adjusting sucrose concentration to 2% and supplement to $50mgL^{-1}$ $NaH_2PO_4$ in 1/2MS medium proved to be more efficient for plant regeneration. Various combinations of growth regulators such as kinetin, BA, NAA, and IBA were added to the growing media, and the best sporophyte regeneration was obtained by $1{\mu}M$ kinetin. The BA addition resulted in vigorous proliferation of meristematic tissues, but without differentiation to sporophytes. Three types of culture methods, solid using agar, liquid stationary, and liquid shaking culture, were employed with or without activated charcoal. The addition of 0.1% activated charcoal to modified 1/2MS media (2% sucrose, $50mgL^{-1}$ $NaH_2PO_4$, $1{\mu}M$ kinetin, pH 5.8 and 0.8% agar) yielded highest sporophyte regeneration in liquid shaking culture.

• Smart Media Journal
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• v.9 no.4
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• pp.81-90
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• 2020

Due to the recent economic development and the improvement of income level in China, the desire for quality medical services is increasing compared to the past. As an alternative to satisfy these needs, various applications using smart phones and the like are being developed. The new corona that occurred in December 2019 began to show great interest in non-face-to-face telemedicine services using smart phones due to the worldwide spread of the coronavirus. Therefore, in this study, a total of 200 people were surveyed on the top three mobile medical applications in China, and the data of 120 people who actually used medical applications were analyzed based on Venkatesh's UTAUT2 theory. A study was conducted on the intent to use and the factors affecting the in-law behavior. First, it has become clear that the interactive characteristics, expectations for effort, price value, interest in privacy, habits, and promotional conditions have a positive impact on the user's use. Second, it was investigated that the user's intention to use influences the behavior of use, and among the intentions of use, it was found that the mobilization characteristic expectation, hedonistic motivation, price value, habits, and promotion conditions affect the use behavior. Third, a study result was derived that the controlling variables such as gender, age, school age, and annual income do not affect the user's intention to use mobile medical applications as a controlling variable. Finally, due to the nature of mobile devices that use the Internet, various security vulnerabilities exist, and this can cause great damage or personal and social impact. Therefore, for the development of mobile medical services in China, it is necessary to re-establish a research model through comprehensive and in-depth considerations to supplement these problems in the future.

• Journal of the Korean Institute of Landscape Architecture
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• v.51 no.3
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• pp.166-178
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• 2023

This study aims to introduce and assess CNN Deep Learning methods to analyze visual landscape images on social media with embedded user perceptions and experiences. This study analyzed visual landscape images by focusing on a healing place. For the study, seven adjectives related to healing were selected through text mining and consideration of previous studies. Subsequently, 50 evaluators were recruited to build a Deep Learning image. Evaluators were asked to collect three images most suitable for 'healing', 'healing landscape', and 'healing place' on portal sites. The collected images were refined and a data augmentation process was applied to build a CNN model. After that, 15,097 images of 'healing' and 'healing landscape' on portal sites were collected and classified to analyze the visual landscape of a healing place. As a result of the study, 'quiet' was the highest in the category except 'other' and 'indoor' with 2,093 (22%), followed by 'open', 'joyful', 'comfortable', 'clean', 'natural', and 'beautiful'. It was found through research that CNN Deep Learning is an analysis method that can derive results from visual landscape image analysis. It also suggested that it is one way to supplement the existing visual landscape analysis method, and suggests in-depth and diverse visual landscape analysis in the future by establishing a landscape image learning dataset.