• Korean journal of communication and information
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• v.63
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• pp.26-52
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• 2013

This study started from the questions on how the selection of the 'New 7 Wonders of Nature' was repeatedly reported, and whose voice was emphasized on which perspective in regional daily newspapers. This is a subject that received the attention of the media with the tag of 'fraud on a national scale'. The results of this study showed that there was no difference among newspapers in all frames excluding the frame of public confidence in relation to the N7W Foundation. Furthermore, after examining the change before and after six media companies' conclusion of MOUs, a significant difference was discovered between newspapers concerning the frame of public confidence pertaining to the N7W Foundation. However, no significant difference was discovered in all frames after the MOUs were concluded. The tone of the press mostly displayed a positive or supporting perspective, and almost no negative or critical reports were published. Second, 68.0% of all the articles displayed use direct quotes. The speakers of direct quotes were mostly from official organizations that initiated the selection event for the 'New 7 Wonders of Nature', and almost no civilians or representatives from civil social groups were quoted. On the other hand, direct quotes of anonymous sources were used rather frequently, and most of these sources were confirmed to be official sources and related parties that initiated the selection event for the 'New 7 Wonders of Nature'. As a result, regional daily newspapers displayed support and advocated for the voices of the entities that initiated the selection event for the 'New 7 Wonders of Nature', including the district government, by emphasizing and repeating their perspectives in the newspaper coverage.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.37-42
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• 2013

Rice is one of the most important cereal crops as a model plant for functional genomics of monocotyledons and usually transformed using Agrobacterium tumefaciens. However, the transformation's process using previous method is still time consuming and uneconomical, low efficiency. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the infection and co-cultivation, Agrobacterium elimination, infected calli's selection steps using liquid media. We directly inoculated Agrobacterium containing a ZjLsL gene under the control of constitutive promoter into the 1- to 3-week-old rice calli derived from mature seeds. After 3 days of co-cultivation, the infected calli were transferred onto liquid media of Agrobacterium elimination and calli's selection for 3 days. The calli were transferred to calli's growth solid media for 14 days and then the calli transferred to shoot induction and root induction media. Putative transformants were initially selected on the medium containing phosphinothricin, and the PAT protein verified by PAT strip test. This method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

• Journal of Plant Biotechnology
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• v.2 no.3
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• pp.143-149
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• 2000

An Efficient in vitro regeneration system and an optimized Agrobacterium mediated transformation protocol are described, based on the use of young seedling cotyledons of Capsicum annuum L. Optimal regeneration efficiency can be obtained by cultivating cotyledon explants on media containing 4 mg/L benzyladenine and 0.1 mg/L indolacetic acid. The effect of antibiotics used to eliminate Agrobacteria, as well as the toxic level of some generally used selection agents (kanamycin, geneticin, hygromycin, phosphinotricin and methotrexate) in regenerating pepper tissues were determined. To enable the comparison of different selection markers in identical vector background, a set of binary vectors containing the marker genes for NPTII, HPT, DHFR and BAR respectively, as well as the CaMV 35S promoter/enhancer-GUS chimaeric gene was constructed and introduced into four different Agrobacterium host strains.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.138-149
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• 2002

Plant cell culture provides a viable alternative over whole plant cultivation for the production of secondary metabolites. In order to successfully cultivate the plant cells at large scale, several engineering parameters such as, cell aggregation, mixing, aeration, and shear sensitivity are taken into account for selection of a suitable bioreactor. The media ingredients, their concentrations and the environmental factors are optimized for maximal synthesis of a desired metabolite. Increased productivity in a bioreactor can be achieved by selection of a proper cultivation strategy (batch, fed-batch, two-stage etc.), feeding of metabolic precursors and extraction of intracellular metabolites. Proper understanding and rigorous analysis of these parameters would pave the way towards the successful commercialization of plant cell bioprocesses.

• Proceedings of the Korean Society of Computer Information Conference
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• 2013.01a
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• pp.223-225
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• 2013

본 논문에서는 카메라가 여러 개 존재하는 다중의 카메라 환경에서 주변의 환경에 최적으로 적합한 카메라를 동적으로 선택하는 알고리즘을 제안한다. 제안된 알고리즘에서는 초기의 입력영상을 받아들인 후, 이 영상으로부터 주위의 환경을 가장 잘 표현할 수 있는 특징인 밝기와 텍스처 특징을 추출한다. 그리고 이전 단계에서 추출된 밝기와 텍스처 특징값들을 가장 잘 반영할 수 있는 카메라를 선택하는 규칙을 생성함으로써 주위 환경에 맞는 카메라를 자동으로 선택해 준다. 본 논문의 실험결과에서는 제안된 방법이 여러 가지 환경에서 잘 동작하며, 결과적으로 주위 환경에 적합한 카메라의 선택을 통해 보다 정확한 3차원의 정보를 추출함을 보여준다.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.562-565
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• 1998

Nisin-producing and nisin resistant L. lactis subsp. lactis ATCC7962 harbored six plasmids. To find a plasmid containing a nisin resistant gene, these plasmids were transformed into L lactis LM0230 of plasmid-free and nisin sensitive strain. After screening on nisin selection media containing nisin (150 $\mu\textrm{g}$/$m\ell$), several nisin resistant transformants were obtained and the level of nisin resistance was very similar to that of wild type L lactis subsp. lactis ATCC7962. A 26.5 kb plasmid, named as pCS100, which confers resistance to nisin, was identified in transformants. The pCS100 was digested with EcoRI and Southern blot hybridization was done with nisI probe to localize the nisin resistant gene. A 4 kb EcoRI fragment showed a strong positive signal, and it was cloned into pBluescript for the potential selection marker.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.07a
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• pp.294-295
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• 2011

The fundamental problem of selective protection scheme for encoded bitstreams is to find an efficient algorithm to select the set of frames required to be encrypted that can maximize protection effect with the minimum amount of protected data is required. In this paper, we propose an optimal selective protection scheme for SVC bitstreams by protecting the best combination of frames for selective protection in the sense that the amount of data required for protection is minimized and the resulting visual quality degradation is maximized. The selection of the frames to be encrypted is done by first expressing R-Q (protection rate - visual quality) relationship with Lagragian cost model. The experimental results show that, compared to protecting SVC bitstreams layer by layer, the proposed scheme gives superior performance in terms of protection effectiveness due to its better selection of frames for protection given protection bit budgets.

• Proceedings of the Korean Society of Computer Information Conference
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• 2011.01a
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• pp.85-88
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• 2011

Fuzzy C-Means (FCM) 알고리듬은 가장 널리 사용되는 군집화 알고리듬 중 하나로 다양한 응용 분야에서 사용되고 있다. 하지만 FCM은 여러 가지 문제점을 가지고 있으며 초기 원형 설정이 그 중 하나이다. FCM은 국부 최적해에 수렴하므로 초기 원형 설정에 따라 클러스터링 결과가 달라진다. 이 논문에서는 이러한 FCM의 초기 원형 설정 문제를 개선하기 위하여 커널밀도 추정 (kernel density estimation) 기법을 활용하는 방법을 제안한다. 제안한 방법에서는 먼저 커널 밀도 추정을 수행한 후 밀도가 높은 지역에 클러스터의 초기 원형을 설정하고 원형이 설정된 영역의 밀도를 감소시키는 과정을 반복함으로써 효율적으로 초기 원형을 설정할 수 있다. 제안된 방법이 일반적으로 사용되는 무작위 초기화 방법에 비해 효율적이라는 사실은 실험결과를 통해 확인할 수 있다.

• Proceedings of the Korea Association of Information Systems Conference
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• 2005.12a
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• pp.381-387
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• 2005

As the most widely used media of BtoB e-business, the e-Marketplace (EM) can play an important role in the age of c-commerce (collaborative commerce). In supply chain management (SCM) area, the relationship-based collaboration among partners has shown great efficiency. Although the collaboration is important in both areas of EM and SCM there has been a critical difference in the selection of trade partners between them. In this paper, the EM's collaborative stages for integration with its customer system are reviewed and a system architecture is proposed for EM's electronic functional role within the perspective of collaborative commerce and buyer-supplier relationship. The relationship-based BtoB commerce through EM is reviewed to explain that it can be more beneficial than the commerce based on the price competitive selection of trade partners. With the proposed system architecture, an EM can be the functional medium for the collaborative IOIS system architecture.

• Journal of Information Processing Systems
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• v.15 no.5
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• pp.1029-1035
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• 2019

In recent years, artificial intelligence (AI) services have become one of the most essential parts to extend human capabilities in various fields such as face recognition for security, weather prediction, and so on. Various learning algorithms for existing AI services are utilized, such as classification, regression, and deep learning, to increase accuracy and efficiency for humans. Nonetheless, these services face many challenges such as fake news spread on social media, stock selection, and volatility delay in stock prediction systems and inaccurate movie-based recommendation systems. In this paper, various algorithms are presented to mitigate these issues in different systems and services. Convolutional neural network algorithms are used for detecting fake news in Korean language with a Word-Embedded model. It is based on k-clique and data mining and increased accuracy in personalized recommendation-based services stock selection and volatility delay in stock prediction. Other algorithms like multi-level fusion processing address problems of lack of real-time database.