• KSBB Journal
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• v.14 no.2
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• pp.241-247
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• 1999

Experimental studies were carried out to optimize the culture conditions of Corynebacterium diphtheriae for the production of diphtheria toxin. A new media which does not contain any meat digest products was selected. The main ingredient of new medium was enzymatic digests of casein known as NZ-Case. In fermenter experiments, the toxin production was increased with the increase of cell growth. The optimum initial pH of media, air flow rate and agitation speed were 7.0, 0.22, vvm and 400 rpm, respectively. The contents of iron and calcium-phosphate precipitate were important for maximal cell growth and toxin production. The optimum concentration of iron was 0.3 mg/L and calcium-phosphate precipitate could serve in gradual supply of iron to maintain the optimal culture condition which is required for enhanced yield of toxin production. In potency test, the potency of toxoid from fermentor culture was higher than that from static culture. When diphtheria toxin is produced by fermentor culture, it is possible to produce higher levels of toxin and better toxoid quality in terms of safety, yield, productivity and immunity.

• The Journal of Engineering Geology
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• v.14 no.2
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• pp.179-187
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• 2004

This study conducted a laboratory work to determine the change of dielectric constant of saturated soil porous media, which is injecting EML to use Frequency Domain Reflectometry(FDR) system and length 7cm-length measurement sensor. It is purpose of study that estimate a movement path through pore of soil for an ethanol mixing liquid(EML) which have the same specific gravity of water at $20{\;}^{\circ}C$, and determine to a dielectric measuring range for the measurement sensor. As an outflow point of saturated soil column upper part recedes from an EML inject point in EML diffusion test, the diffusion extent of EML through pore of saturated soil was expanded. And results of ail EML flow experiment were measured the change of dielectric constant at all measurement sensors which are placed to constant interval at the same travel time for saturated soil column. Therefore, the displace process of water that exist in pore of saturated soil by EML injection is enough available and confirm together mobility through pores.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.5
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• pp.1397-1403
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• 2004

By recently study, GM (Gamipaemo-tang) treatment have worked well on the allergic asthma. The purpose of this study was effect of GM extract on helper T cell, major regulator of immune system. Splenic cells from 8-week BALB/c mice were cultured in GM containing media without activation for 48 hours. The MTS assay and flow cytometry study revealed that lymphocyte treated with GM were not effective on CD4+ T cells. Subsequently CD4+ T cells were isolated and cultured in GM containing media. Either GM were not effective on CD4+ T cell without APCs. By FACS scan analysis, the expression of INF-γ, IL-4 were down-regulated in the condition skewed Th1 and Th2 cells respectively, Using ELISA analysis, the expression of INF-γ is up-regulated and IL-4 is down-regulated in the condition skewed Th1, Th2 cells respectively. With RT-PCR analysis, the expression of mRNA for INF-γ is down-regulated and IL-4 is down-regulated in the condition skewed Th1 and Th2 cells respectively. The result suggests that GM inhibited the differetiation of Th2 cells significantly and indicates GM could enhance anti-allergic immune system.

• Journal of Korea Multimedia Society
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• v.14 no.7
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• pp.905-916
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• 2011

Recently, the W3C has developed the HTML 5 standard to provide various multimedia services as well as a web based streaming. HTML5 based web browsers with the built-in video codecs can be used directly for streaming services, though previous web browsers should contain playback plug-in for streaming services. In addition, an adaptive streaming technology using HTTP/TCP has been standardized, since an Internet firewall does not support RTP/UDP streaming protocol but HTTP/TCP streaming protocol. In this paper, we propose the service migration mechanism among various devices in the HTML5-based adaptive HTTP streaming environments. The proposed algorithm and architecture were modeled for migrating media flows, media flow control, and session control and implemented using JavaScript. Finally, we showed that the proposed mechanism was effective to migrate HTTP streaming service among various devices by performing several experiments.

• The Journal of the Korea Contents Association
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• v.14 no.1
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• pp.139-149
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• 2014

This study attempts to explore the inter-media agenda setting effect between television and Twitter based on the framework of the two-step flow theory. Twitter's increasingly important role in political communication can be effectively addressed by examining the process by which Twitter users form their opinions on television debate program. Content analyses of Twitter discussions after television debate of the Korean presidential candidates provided interesting insights into how Twitter's opinion leaders reflect on the televised debates. The results show that Twitter mentions rather focus on personality traits of the candidates while television debates emphasize the candiates' policy issues. Specifically, Twitter users mainly concentrated on the political ideology and morality of the candidates. In sum, Twitter seems to have its own way of influencing the public opinion separately from the television.

• Journal of The Korean Society of Agricultural Engineers
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• v.60 no.2
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• pp.25-36
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• 2018

Drip irrigation system is a low energy cost method which can efficiently save and supply water by dropping water slowly on the crop's root zone during crop growth. In the drip irrigation system, disk filters take an important role to physically remove impurity (inorganic and suspended organic) particles present in agricultural water which can cause emitter clogging. For the purpose, both top-and-bottom surfaces of the disk are grooved in micron size flowing from outside to inside. However, many congested flow paths in disk filter media incur higher head loss of inflow water resulting in relatively decreasing velocities depending on operation time than sand and mesh filters. Therefore, it is important to optimize the structure of disk filter in micro irrigation system. The head loss of disk filter media takes also charge of more than 60 % of total head loss in whole disk filter. This study is to find the appropriate cross-sectional shape of the disk groove to minimize the head loss by executing the experiment. The experiment used three disk filters that have similar filter body but have a half-elliptic and two kinds of triangular cross sections. The experimental results showed that the disk filter with half-elliptic cross sections of disk grooves have less head loss than the disk filter with regular triangular one.

• Journal of Korean Society on Water Environment
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• v.24 no.3
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• pp.273-279
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• 2008

Space separation method that use independent reactor for nitrification and other reactor for denitrification has been commonly used for biological nitrogen removal process like $A^2O$ process. However, this method needs large space and complicate pipelines and time separation method such as SBR process have a difficulty in continuous treatment. Thus biological nitrogen removal process which is capable of continuous treatment, easy opeation and space saving is urgently required. In this research, submerged moving media was used for a biofilm process and suspended sludge was used for biological nitrogen removal at the same time. In particular DO environment by controlling air flow rate was investigated for simultaneous nitrification/denitrification. Total nitrogen removal in aeration rate more than $67L/min{\cdot}m^3$ showed 51~53% and rose to 65%, 70% and 78% in $50L/min{\cdot}m^3$, $58L/min{\cdot}m^3$ and $25L/min{\cdot}m^3$ respectively. Total phosphorus removal was very low about 10~20% more than $67L/min{\cdot}m^3$ aeration rates. But total phosphorus removal roses when reduces aeration rate by $58L/min{\cdot}m^3$ low and it showed total phosphorus removal of 72% in aeration rate $25L/min{\cdot}m^3$.

• International Journal of Advanced Culture Technology
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• v.8 no.3
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• pp.61-72
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• 2020

The fear of crime, discussed in the early 1960s in the United States, is a psychological response, such as anxiety or concern about crime, the potential victim of a crime. These anxiety factors lead to the burden of the individual in securing the psychological stability and indirect costs of the crime against the society. Fear of crime is not a good thing, and it is a part that needs to be adjusted so that it cannot be exaggerated and distorted by the policy together with the crime coping and resolution. This is because fear of crime has as much harm as damage caused by criminal act. Eric Pawson has argued that the popular impression of violent crime is not formed because of media reports, but by official statistics. Therefore, the police should watch and analyze news related to fear of crime to reduce the social cost of fear of crime and prepare a preemptive response policy before the people have 'fear of crime'. In this paper, we propose a deep - based news classification system that helps police cope with crimes related to crimes reported in the media efficiently and quickly and precisely. The goal is to establish a system that can quickly identify changes in security issues that are rapidly increasing by categorizing news related to crime among news articles. To construct the system, crime data was learned so that news could be classified according to the type of crime. Deep learning was applied by using Google tensor flow. In the future, it is necessary to continue research on the importance of keyword according to early detection of issues that are rapidly increasing by crime type and the power of the press, and it is also necessary to constantly supplement crime related corpus.

• The Journal of the Korean dental association
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• v.46 no.9
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• pp.563-573
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• 2008

Purpose : The purpose of this study is to investigate the effects of Simvastain, which is HMG-CoA reductase inhibitor, on proliferation and differentiation of osteoblast. Materials & Methods : Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x $10^4$ cells per plate. Each plates were incubated with 5% $CO^2$incubator $37^{\circ}C$. Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, $100\muM$ of Simvastatin were added with Osteogenesis induction media, and classified as "test group". Those not added with Simvastatin were classified as "control group". Results : 1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than $0.1\muM$. 2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than $0.1\muM$. 3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than $0.1\muM$. 4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group. Conclusion : From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts.

• International journal of advanced smart convergence
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• v.9 no.1
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• pp.63-69
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• 2020

The rapid development in modern industrialization pollutant the water and atmospheric air across the globe that have a major impact on the human and livings health. In worldwide, every country government increasing the importance to improve the outdoor air pollution monitoring and control to provide quality of life and prevent the citizens and livings life from hazard disease. We proposed the environmental dust level detection method for outdoor facilities using sensor fusion technology to measure precise micro-dust level and monitor in realtime. In this proposed approach use the camera sensor and commercial dust level sensor data to predict the micro-dust level with data fusion method. The camera sensor based dust level detection uses the optical flow based machine learning method to detect the dust level and then fused with commercial dust level sensor data to predict the precise micro-dust level of the outdoor facilities and send the dust level informations to the outdoor air pollution monitoring system. The proposed method implemented on raspberry pi based open-source hardware with Internet-of-Things (IoT) framework and evaluated the performance of the system in realtime. The experimental results confirm that the proposed micro-dust level detection is precise and reliable in sensing the air dust and pollution, which helps to indicate the change in the air pollution more precisely than the commercial sensor based method in some extent.