• Tuberculosis and Respiratory Diseases
• /
• v.56 no.4
• /
• pp.381-392
• /
• 2004

Arsenic trioxide($As_2O_3$) was introduced into the treatment of refractory or relapsed acute promyelocytic Ieukemia. Some investigators have reported that arsenic trioxide had induced apoptosis in a variety of solid human tumor cell lines, including non-small cell lung cancer. Non-steroidal anti-inflammatory drugs(NSAIDs) are powerful chemopreventive agents for gastrointestinal cancers and the growth of established tumors are reduced by inducing apoptosis. It's also reported that NSAIDs enhanced tumor response to chemotherapeutic drugs or radiation. In this study, we aimed to determine whether combination of arsenic trioxide with sulindac augmented its apoptotic potential in NCI-H157 human lung cancer cells. The human lung cancer cell line NCI-H157 was treated with arsenic trioxide and sulindac. Cell viability was measured by the MTT assay. Apoptosis was measured by nuclear staining and flow cytometric analysis. The catalytic activity of the caspase families were measured by the fluorogenic cleavage of biosubstrates. The western blotting were also performed to define the mechanical basis of apoptosis. Combination treatment of arsenic trioxide and sulindac decreased the viability of NCI-H157 human lung cancer cells in a dose-dependent manner. The catalytic activity of caspase-3, 8 and 9 proteases were increased after combination treatment. Consistently PARP was cleaved from 116kDa to 85kDa fragments, and the expression of ICAD was decreased by time-dependent manner. Also combination treatment increased the expression of Fas and Fas/L. Combination therapy of arsenic trioxide with sulindac augments cell death and induces apoptosis via the activation of caspase cascade in NCI-H157 human lung carcinoma cells.

• Transactions of the KSME C: Technology and Education
• /
• v.3 no.1
• /
• pp.23-28
• /
• 2015

In this paper, we performed the design optimization dual-shell and tube heat exchanger on exhaust waste heat recovery for gas heat pump using CFD and RSM. CFD analysis is useful to design the complex structure such as double shell and tube heat exchanger. By computer simulation, engineers can assess the feasibility of the given design factors and change them to get a better design. But if one wishes to perform complex analysis on the simulation, such dual-shell and tube heat exchanger for GHP, the computational time can become overwhelming. CFD is powerful but it takes a lot of time for complex structure. Therefore, the CFD analysis is minimized by the optimization using the RSM method. As a result, the number of baffle and tube are optimized by 6 baffles and 25 tubes for heat transfer and flow friction. And then pressure drop and heat transfer is improved about 12.2%. We confirm the design optimization using CFD and RSM is useful on complex structure of heat exchanger.

• Journal of Acupuncture Research
• /
• v.30 no.3
• /
• pp.125-134
• /
• 2013

Objectives : We studied the effects of electro and laser acupuncture treatment with $GB_{39}$ and $GB_{34}$ on neuropathic pain in rats induced by tibial and sural nerve ligation. Methods : To produce the model of neuropathic pain, the tibial and sural nerves of rats were ligated by a 6-0 silk thread. Three days after the neuropathic surgery, only electro acupuncture(EA), electro acupuncture and 830 nm laser acupuncture(EA-LA-1), and electro acupuncture and 904 nm laser acupuncture(EA-LA-2) were treated with $GB_{39}$ and $GB_{34}$ twice a week for 8 weeks. We observed the withdrawal response of neuropathic rats' legs by von Frey filament and acetone stimulation. We also observed c-fos and nocieptin on the central gray area in the midbrain of neuropathic rats. Results : As we observed the effect of mechanical allodynia, the EA and EA-LA-1 groups in 5 and 6 weeks and the EA-LA-2 group in 6 weeks increased significantly compared with the control group. As for the effect of c-fos activity in the central gray region, the EA, EA-LA-1, and EA-LA-2 groups decreased significantly compared with the control group. The EA-LA-2 group increased significantly compared with the control group as regards the effect of nociceptin activity in the central gray region. Conclusions : We noticed the synergic effect of electro and laser acupuncture treatment because the EA-LA-1 and EA-LA-2 groups had more controllable effect compared with the control group. This study can be used in clinical therapy for neuropathic pain.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.11 no.3 s.55
• /
• pp.53-62
• /
• 2007

This paper presents a new vibration control method for long-span bridges using complex damper system. The new system presents simple mechanical configuration with oil and elasto-plastic dampers which have velocity and displacement dependency in vibration energy absorbing. This system can produce various damping forces according to the applied external forces by the velocity and displacement-dependent characteristics of the dampers. The oil damper dissipates vibration energy for relatively frequent and small amplitude like in the case for small to moderate earthquakes, whereas the elasto-plastic damper system works for rare and large amplitude vibration such as high seismic excitation. Thus, the proposed system exhibits the advantage of low cost with high performance since the roles of the two different dampers are effectively separated. A numerical model is established for the complex damper system, and the response characteristics and effectiveness of the proposed system are presented through numerical simulations. Numerical results show that the proposed complex damper system can significantly improve the seismic performance of long-span bridge structures with much more effective damping mechanism than single conventional passive damper systems.

• Journal of the Society of Naval Architects of Korea
• /
• v.54 no.2
• /
• pp.151-160
• /
• 2017

Growing demand and rapid development of the synthetic fiber rope in mooring system have taken place since it has been used in deep water platform lately. Unlike a chain mooring, synthetic fiber rope composed of lightweight materials such as Polyester(polyethylene terephthalate), HMPE(high modulus polyethylene) and Aramid(aromatic polyamide). Non-linear stiffness and another failure mode are distinct characteristics of synthetic fiber rope when compared to mooring chain. When these ropes are exposed to environmental load for a long time, the length of rope will be increased permanently. This is called 'the creep phenomenon'. Due to the phenomenon, The initial characteristics of mooring systems would be changed because the length and stiffness of the rope have been changed as time goes on. The changed characteristics of fiber rope cause different mooring tension and vessel offset compared to the initial design condition. Commercial mooring analysis software that widely used in industries is unable to take into account this phenomenon automatically. Even though the American Petroleum Institute (API) or other classification rules present some standard or criteria with respect to length and stiffness of a mooring line, simulation guide considers the mechanical properties that is not mentioned in such rules. In this paper, the effect of creep phenomenon in the fiber rope mooring system under specific environment condition is investigated. Desiged mooring system for a Mobile Offshore Drilling Unit(MODU) with HMPE rope which has the highest creep is analyzed in a time domain in order to investigate the effects creep phenomenon to vessel offset and mooring tension. We have developed a new procedure to an analysis of mooring system reflecting the creep phenomenon and it is validated through a time domain simulation using non-linear mooring analysis software, OrcaFlex. The result shows that the creep phenomenon should be considered in analysis procedure because it affects the length and stiffness of synthetic fiber rope in case of high water temperature and permanent mooring system.

• Journal of Korean Home Economics Education Association
• /
• v.14 no.2
• /
• pp.37-52
• /
• 2002

This study was made on the concerns and implementation of the ICT(Inormation and Communication Technology)-utilized lesson by home economics teacher. The objective of this study is to investigate the stages of concern and the level of use of the ICT-utilized lesson by home economics teachers in Kyunggi Province through the concerns based adoption model(CBAM) and to provide assistance for ICT-utilizing lesson to be efficiently adopted to home economics. This study made selected 200 schools by random sampling among 233 middle schools which have two or more home economic teachers and 21 or more classes in Kyunggi Province which has a total of 395 middle schools and mail-surveyed on 400 home economics teachers by means of questionnaire. The stages of concern. the levels of use and the types of implementation were used as instruments in this survey. The results of this study on the stages of concern. the levels of use and the type of implementation of the ICT-utilized lesson by home economics teachers in Kyunggi Province were as following: First. the highest point of the concern of home economics teachers of ICT-utilized lesson was the stage of awareness. the second highest point was the stage of management. the 3rd stage. and the stage of information. the lst stage. Second. the highest level of implementation of ICT-utilized lesson by home economics teachers was the level of mechanical use. the 3rd stage(30.4％), which followed by the level of orientation. the 1st stage(22.5％). and the level of nonuse(16.7％). the level of rountine use. the 4th stage(13.7％) the level of integration. the 5th stage(11.8％). the level of preparation 2(3.9％). and the level of renewal. the 6th stage(1.0％) Third, information search was the most in the type of ICT-use and in the course of lesson CD-ROM was used the most.. During ICT-utilized lesson. most of teachers used computer one to two hours a week mainly in the lessons of clothing life and eating life. Home economics teachers took the most training of how to use word-processor(68.6％) during computer education. and 60 teachers(66.0％) gave positive response about the effect of computer education on teacher's learning. Finally. the biggest problem with ICT-use in the teacher's learning was the long preparation time for lesson. and problem with ICT-utilized lesson was the burden of time and effort spent to buy needed materials and to recompose the existing materials for the lesson. Therefore. so as to adopt ICT-utilized lesson efficiently into school it is needed that active promotion for the lesson should be made to teachers. training teachers to raise their ability to use computer and various kinds of software should be expanded. and school authorities' financial and administrative assistance should be given for the smooth proceeding of the lesson.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.12 no.2
• /
• pp.821-828
• /
• 2011

This paper developed the algorithm for improving the performance the auto pilot in the autonomous vehicle system consisting of the Track keeping control, the Automatic steering, and the Automatic mooring control. The automatic steering is the control device that could save the voyage distance and cost of fuel by reducing the unnecessary burden of driving due to the continuous artificial navigation, and avoiding the route deviation. During the step of the ship autonomic navigation control, since the wind power or the tidal force could make the ship deviate from the fixed course, the automatic steering calculates the difference between actual sailing line and the set course to keep the ship sailing in the vicinity of intended course. first, we could get the transfer function for the modeling of ship according to the Nomoto model. Considering the maneuverability, we propose it as linear model with only 4 degree of freedoms to present the heading angle response to the input of rudder angle. In this paper, the model of ship is derived from the simplified Nomoto model. Since the proposed model considers the maximum angle and rudder rate of the ship auto pilot and also designs the Fuzzy controller based on existing PID controller, the performance of the steering machine is well improved.

• Applied Microscopy
• /
• v.39 no.3
• /
• pp.261-266
• /
• 2009

This study aim to disclose a possible mechanism for the neuronal cell death induced by peripheral nerve injury following a spinal nerve ligation (SNL) as a neuropathic pain model. Male Sprague-Dawley rats (270~290 g) were used for this study. Pain threshold was evaluated for their response to mechanical (von Frey hairs) stimuli 1, 3, and 7 days after a tight ligation of L5 ventral ramus. In control group, the small ganglion cells were strongly stained with routine toluidine blue (TB), whereas the large ganglion cells showed a little bit weak stainity. Each large ganglion cell is surrounded by perineuronal satellite cells. In experimental groups, small ganglion cells showing apparent degenerative changes increased on 1 day, and showed a peak in degenerative cell number at 3 days group, and decreased gradually at 7 days group. We also found a small number of large-sized ganglion cells showing mild degenerative changes. However their satellite cells ware relatively intact with no typical findings throughout this experiment. Under the electron microscope, small ganglion cells showed various stage and typical features of the dark degeneration including mitochondrial swelling.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.6 no.2
• /
• pp.3-15
• /
• 2003

Recently more than 450 incidents of oil spill a year have occurred in nearshore of Korea, which caused unmeasurelable losses in fisheries and severe damage in marine ecosystem. Two approaches remain paramount in any response to marine oil spill : the enhancement of natural dispersion of the oil by using dispersants, and mechanical recovery using booms and skimmers. A technique currently receiving fresh attention is the enhancement of the natural bioremediation of oil through the application of micro-organisms and/or nutrient. Oil, like many natural substances, will biodegrade over a period of time into simple compounds such as carbon dioxide, water and biomass. Bioremediation is the term used to describe a range of processes which can be used to accelerate natural biodegradation. More specifically biostimulation is the application of nutrients, and bioaugremetation or seeding is the addition of microbes specially selected to degrade oil. Bioremediation is an economically attractive method for the clean-up of oil-contaminated area. Bioremediation has been demonstrated to be an effective oil spill countermeasure for use in cobble, sand beach, salt marsh, and mud flat environment.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.9
• /
• pp.6425-6431
• /
• 2015

Invertase, that hydrolyzes sucrose into glucose and fructose, plays a great role in carbohydrate reallocation between the photosynthetic source tissue and various sink tissues. Invertase also occurs in a variety of isoforms for various functions in plants. Insoluble invertases were extracted only in buffer solutions containing high concentrations of salt. Within these classes, acid invertase has an optimum activity at acidic pH (pH 4-5). Induction of insoluble acid invertase (INAC-INV) in leaf, stem, and root tissues in response to physical wounding has been investigated. To detect the localization of INAC-INV within the plant, immunolocalization has been performed. In this study, the accumulation of INAC-INV was noticeable to reach maximum levels on 72 hr after mechanical injuries. INAC-INV was induced in wounded leaves 3 times more than control leaves. Immunolocalization results showed that INAC-INV accumulated in wall appositions and intercellular spaces. INAC-INV was also localized at sieve cell walls in phloem tissues close to the site of wounding. Taken together, this study suggested that INAC-INV induction upon wounding injuries can play a role on responses to the high energy demand for wound healing process.