• 한국응용곤충학회지
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• 제60권4호
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• pp.387-401
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• 2021

시설재배지를 대상으로 고추 정식 이후 황색 끈끈이트랩으로 총채벌레 발생을 모니터링하였다. 아울러 토마토반점위조바이러스(Tomato spotted wilt virus: TSWV)가 유발하는 고추 칼라병을 유관으로 조사하였다. 고추 정식 직후(3월 말) 낮은 밀도로 대만총채벌레(Frankliniella intonsa)가 트랩에 포획되었으며 4월 중순부터는 꽃노랑총채벌레(Frankliniella occidentalis)도 발견되었다. 이후 5월부터는 두 종이 전체 총채벌레의 98% 이상을 차지하였고, 이 가운데 대만총채벌레가 꽃노랑총채벌레보다 다소 많은 발생 밀도를 보였다. 전체 총채벌레의 발생 피크를 보면 5월 중순에 낮은 피크를 기점으로 6-7월에 발생 최성기를 보였다. 이후 총채벌레의 발생은 급격하게 감소하였다. 포획된 꽃노랑총채벌레의 암수 비율이 일정하지 않았는데 이는 이 곤충의 특이적 단성생식 가능성으로 이에 대한 실험적 증거를 제공하였다. 지역간 꽃노랑총채벌레의 유전적 거리를 COI 서열로 비교한 결과 원거리에서 채집한 꽃노랑총채벌레 집단과는 차이를 보였지만 안동지역 내에서 발생한 꽃노랑총채벌레는 COI 서열에서 높은 유사성을 보였다. 이들 주요 두 종의 총채벌레가 전파할 것으로 추정되는 고추 칼라병이 일부 시설재배지를 중심으로 발견되었으며 항혈청 및 분자진단을 통해 확인되었다. 더불어 감염 고추에서 채집된 꽃노랑총채벌레에서도 분자진단을 통해 TSWV를 검출하였다. 감염 TSWV의 게놈 구조를 비교하기 위해 기능성 단백질을 갖는 NS_S, N, NS_M의 유전자 서열을 분석하였다. 서로 다른 지역별 이들 유전자는 다수의 점돌연변이가 존재하였고 이들 가운데는 아미노산 서열 차이를 초래하는 오류 돌연변이를 포함하였다. 추출된 TSWV를 비보독충 꽃노랑총채벌레에 섭식 처리한 유충과 성충 모두에서 감염으로 일어났으나, 유충에게서만 바이러스 증식이 일어나는 것을 확인하였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.90.2-91
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• 2003

In commercial greenhouses, senescent flower petals or flowers of vegetables such as tomato, strawberry, hot pepper and zucchini squash were blighted to be removed from fruits within five days after spraying of Trichoderma harzianum YC459 (TORY), a biocontrol agent for the gray mold rot of vegetables caused by B. cinerea The mechanism for selective colonization of senescent floral tissues by T. harzianum YC459 was elucidated using fresh and senescent (Hays and 14days after flowering, respectively) floral tissues of zucchini squash (Cucurbita moschata Duchesne). The spores of T. hrzianum YC459 were produced more on agar and liquid culture media supplemented with 5％ dry powder of senescent floral tissues than fresh tissues during 15days. Mycelial growth was also much better in the media with senescent tissues than with fresh tissues. Enzyme activities of amylase, polygalacturonase and cellulase in the liquid media which might be involved in the colonization of tissues by T. harzianum YC459 were compared. The activities of three enzymes were much higher in the media with senescent floral tissues than with fresh floral tissues reaching to the maximum during 9 to 12days of incubation. Based on the results, the removal of senescent floral tissues, a possible inoculum source of the pathogen, may be another mechanism for biocontrol of gray mold rot of vegetables by T. harzianum YC459.

• 한국유기농업학회지
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• 제28권3호
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• pp.335-346
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• 2020

The objective of this study is to analyze the consumer perception of reusable wreath and present the considerations for introducing the labeling system, and to calculate the social benefits and costs of that. For this, We did an internet survey on the perceptions and willingness-to-pay for reuse wreath to 330 adults. As a result, only 56.1 percent of those who ordered wreath were found to check whether they actually arrived. 73.6% of the respondents had the willingness-to-purchase a reusable wreath if the price was reasonable, and 43.6% were in favor of labeling reused wreath as 'reused wreath'. The social benefits of introducing the reusable currency marking system are about 155,692 million won and the social cost about 43.52 million won. If reusable wreath are not recognized and purchased at the price of new one, economic and emotional losses of domestic horticulture farmers and consumers may occur. Therefore, related agencies will be able to expand sales of domestic flower producers and protect the economic and emotional benefits of consumers if they make efforts to establish and operate a reusable wreath labeling system.

• The Plant Pathology Journal
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• 제17권4호
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• pp.231-235
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• 2001

From April to May 1998, Phytophthora rot on pear, which has not been reported in Korea before, became an epidemic in the southeast part of the country under abnormally higher temperature and prolonged rainy days. Average temperature was about $3^{\circ}$ higher than in normal years, and 29 days were rainy during the 2 months in the areas surveyed. Over 1,000 orchards estimated at about 270 ha in 19 cultivation areas were infected by the disease, which occurred on all parts of the tree such as leaves, shoots, branches, stems, and flower clusters. Among 43 isolates collected from various locations and plant parts, 41 were identified as Phytophthora cactorum while 2 were identified as P. cambivora based on their mycological characteristics. The representative isolates revealed strong pathogenicity not only to pear but also to apple and peach. Among 23 pear cultivars tested, 7 were estimated as susceptible, 4 were moderate, and 11 were resistant to the pathogen. Results suggest that Phytophthora disease on pear is a potential threat to pear cultivation when environmental factors are favorable to disease development.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.167-172
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• 2007

A tomato zinc-finger protein gene, LeZFP1, encoding the Cys2/His2-type zinc-finger transcription factor was searched from cDNA microarray analysis of gene expression following induction of the overexpressed tomato transgenic plants showing resistance for pathogen and abiotic stresses. The full-length cDNA of LeZFP1 encoded a protein of 261 amino acid residues. Analysis of the deduced amino acid sequence of LeZFP1 revealed that it shares high sequence identity with pepper CAZFP1 (81% identity). We found that single copy of LeZFP1 gene is present in the tomato genome through southern blot analysis. The LeZFP1 transcripts were constitutively expressed in the tomato mature and young leaves, but were detectable weakly in the flower, stem and root. The LeZFP1 transcripts were significantly reduced in treated leaf tissues with NaCl and mannitol. The LeZFP1 gene was induced by oxidative stress especially. Our results indicated that LeZFP1 may play a role function involved in oxidative stress signaling pathways.

• 한국조경학회지
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• 제28권6호
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• pp.127-132
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• 2001

The purpose of this research is to compare and analyze scientific names and Korean names of interior plants, considering that consumes' purchasing systems consist of internet and communication media in the era of informationalization, to help the consumers purchasing plants, and to propose some directions for the unification of Korean name in practical circulation. This survey was performed during 4 months from May to September in 2000. The survey places for research were wholesale flower market of YangJje Dong and Nam Seoul. The used plants for research were 37 family, 76 genus and 120 species. The main methods of data collection were interview and reference f plants picture books. The main reference for identifying plant name was accomplished by Yoon Ypung-Sub's HORTUS KOREANA. The research results are as follows; 1) Fifty one plants were identified as using generic or specific name. 2) Thirteen plants were identified as using varietal name. 3) Twenty four plants were identified as using common name recoded in a plant dictionary. 4)Thirteen plants were identified as using incorrect name. Consequently, it was identified that the research for the unification of Korean name of interior landscape plants should be performed thorough continuous cooperation between academic field and business field for solving various problems which is occurred in plants circulation process.

• Journal of Astronomy and Space Sciences
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• 제29권1호
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• pp.47-49
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• 2012

Massive binary stars lose mass by two mechanisms: jet-driven mass loss during periods of active mass transfer and by wind-driven mass loss. Beta Lyrae is an eclipsing, semi-detached binary whose state of active mass transfer provides a unique opportunity to study how the evolution of binary systems is affected by jet-driven mass loss. Roche lobe overflow from the primary star feeds the thick accretion disk which almost completely obscures the mass-gaining star. A hot spot predicted to be on the edge of the accretion disk may be the source of beta Lyrae's bipolar outflows. I present results from spectropolarimetric data taken with the University of Wisconsin's Half-Wave Spectropolarimeter and the Flower and Cook Observatory's photoelastic modulating polarimeter instrument which have implications for our current understanding of the system's disk geometry. Using broadband polarimetric analysis, I derive new information about the structure of the disk and the presence and location of a hot spot. These results place constraints on the geometrical distribution of material in beta Lyrae and can help quantify the amount of mass lost from massive interacting binary systems during phases of mass transfer and jet-driven mass loss.

• BMB Reports
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• 제42권1호
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• pp.28-34
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• 2009

To understand the molecular mechanism underlying proline accumulation in Brassica napus, cDNAs encoding ${\Delta}^1$-pyrroline-5-carboxylate synthetase (BnP5CS), ornithine $\delta$-aminotransferase (BnOAT) and proline dehydrogenase (BnPDH) were isolated and characterized. Southern blot analysis of BnP5CSs in B. napus and its diploid ancestors suggested a gene loss may have occurred during evolution. The expression of BnP5CS1 and BnP5CS2 was induced, while the expression of BnPDH was inhibited under salt stress, ABA treatment and dehydration, prior to proline accumulation. The upregulation of BnOAT expression was only detected during prolonged severe osmotic stress. Our results indicate that stress-induced proline accumulation in B. napus results from the reciprocal action of activated biosynthesis and inhibited proline degradation. Whether the ornithine pathway is activated depends on the severity of stress. During development, proline content was high in reproductive organs and was accompanied by markedly high expression of BnP5CS and BnPDH, suggesting possible roles of proline during flower development.

• Natural Product Sciences
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• 제9권4호
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• pp.235-240
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• 2003

Tectorigenin has an apoptosis-inducing ability and immunosuppressive activity. We investigated the effect of tectorigenin on Phase I and II enzyme activities to elucidate the pharmacological action of the immunosuppressive tectorigenin in the diabetic rat. This compound was obtained from the hydrolysis of tetoridin isolated from the flower of Pueraria thumbergiana (Leguminosae). This crude drug (Puerariae Flos) has been used as a therapeutic for diabetes mellitus in traditional Korean medicine. Tecotrigenin inhibited the formation of malondialdehyde (MDA) and hydroxy radicals in serum and liver but promoted superoxide dismutase (SOD) activity. Low MDA contents and low xanthine oxidase and aldehyde oxidase activities were observed in the tectorigenin-treated rats, suggesting that such Phase I enzyme activities are the major source of lipid peroxidation. However, tectorigenin increased Phase II enzyme activities such as SOD, glutathione peroxidase and catalase, suggesting the activation of free radical-scavenging enzymes. The activities of tectorigenin were comparable to those of glibenclamide, which was employed as a positive control. These results suggest that tectorigenin may share some biological properties with glibenclamide in insulin-dependent-diabetes mellitus (IDDM).

• 대한화장품학회지
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• 제27권1호
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• pp.73-81
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• 2001

For an attempt to develop safe materials protecting UV-induced skin damage, plant extracts were evaluated for their antioxidative and free radical scavenging activities. From the results of these screening procedures, the ethanol extract of the flowers of Prunus persica was selected for further study. It was found that Prunus persica (50-200 $\mu\textrm{g}$/㎖) inhibited UVB-induced DNA damage measured by tail moment in the Single Cell Gel Electrophoresis(COMET assay) and inhibited UV-induced lipid peroxidation, expecially against UVB-induced peroxidation at higher than 10 $\mu\textrm{g}$/㎖. Also P.persica(100∼1,000 $\mu\textrm{g}$/㎖) inhibited the amount of $\^$14/C-arachidonic acid metabolites release from UVB-irradiated keratinocytes and it possessed the protective activity against UV-induced cytotoxicity of keratinocytes. All these results indicate that the flowers of P. persica extract may be beneficial for protection UV-induced skin damage when topically applied.