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http://dx.doi.org/10.5656/KSAE.2021.11.0.037

Thrips Infesting Hot Pepper Cultured in Greenhouses and Variation in Gene Sequences Encoded in TSWV  

Kim, Chulyoung (Department of Plant Medicals, Andong National University)
Choi, Duyeol (Department of Plant Medicals, Andong National University)
Kang, Jeong Hun (Department of Plant Medicals, Andong National University)
Ahmed, Shabbir (Department of Plant Medicals, Andong National University)
Kil, Eui-Joon (Department of Plant Medicals, Andong National University)
Kwon, Gimyeon (Biological Utilization Institute, Inc.)
Lee, Gwan-Seok (Crop Protection Division, National Institute of Agriculture and Sciences)
Kim, Yonggyun (Department of Plant Medicals, Andong National University)
Publication Information
Korean journal of applied entomology / v.60, no.4, 2021 , pp. 387-401 More about this Journal
Abstract
Thrips infesting hot peppers were monitored in greenhouses using yellow sticky traps. In addition, the hot peppers infected with tomato spotted wilt virus (TSWV) were observed during the monitoring period. The flower thrips (Frankliniella intonsa) were initially trapped at a low density just after transplanting seedlings of hot peppers at late March. The western flower thrips (Frankliniella occidentalis) were trapped after mid April. These two thrips represented more than 98% of the total thrips attracted to the traps after May, in which F. intonsa showed higher occurrence frequency than F. occidentalis. The total number of thrips had two peaks at mid May with a small and short-term peak and at June-July with a large and long-term peak. The trapped thrips exhibited inconsistent sex ratios, suggesting a seasonal parthenogenesis. Different geographical populations were varied in cytochrome oxidase I sequences, in which local populations in Andong shared a high sequence similarity. TSWV-infected hot peppers, which might be mediated by these two thrips species, were observed and confirmed by an immunoassay kit and a molecular diagnosis using RT-PCR. In addition, the TSWV was detected in F. occidentalis collected from the infected hot peppers. Three open reading frames (NSS, N, and NSM) of the isolated TSWV genomes were sequenced and showed multiple point mutations containing missense mutations among geographical variants. When the isolated TSWV was fed to nonvirulent thrips of F. occidentalis, the virus was detected in both larvae and adults. However, the viral replication occurred in larvae, but not in adults.
Keywords
Hot pepper; Western flower thrips; TSWV; Genome; Variation;
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