• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.712-720
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• 2007

To investigate clonal variations of recombinant Chinese hamster ovary(rCHO) clones in response to culture pH and temperature, serum-free suspension cultures of two antibody-producing CHO clones(clones A and B), which were isolated from the same parental clone by the limiting dilution method, were performed in a bioreactor at pH values in the range of 6.8-7.6, and two different temperatures, $33^{\circ}C\;and\;37^{\circ}C$. In regard to cell growth, clone A and clone B displayed similar responses to temperature, although their degree of response differed. In contrast, clones A and B displayed different responses to temperature in regard to antibody production. In the case of clone A, no significant increase in maximum antibody concentration was achieved by lowering the culture temperature. The maximum antibody concentration obtained at $33^{\circ}C$(pH 7.4) and $37^{\circ}C$(pH 7.0) were $82.0{\pm}2.6$ and $73.2{\pm}4.1{\mu}g/ml$, respectively. On the other hand, in the case of clone B, an approximately 2.5-fold increase in maximum antibody concentration was achieved by lowering the culture temperature. The enhanced maximum antibody concentration of clone B at $33^{\circ}C$($132.6{\pm}14.9{\mu}g/ml$ at pH 7.2) was due to not only enhanced specific antibody productivity but also to prolonged culture longevity. At $33^{\circ}C$, the culture longevity of clone A also improved, but not as much as that of clone B. Taken together, CHO clones derived from the same parental clone displayed quite different responses to culture temperature and pH with regards antibody production, suggesting that environmental parameters such as temperature and pH should be optimized for each CHO clone.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.478-483
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• 1997

Six adult female Murrah buffaloes of about 12 years were exposed to solar radiation during summer when minimum and maximum ambient temperatures were 27.1 and $44.1^{\circ}C$, respectively. The skin surface temperature at forehead, middle pinna, neck, rump, foreleg, hind legs were recorded using non-contact temperature measuring instrument and respiration rate and rectal temperature were measured throughout the 24 hours starting from 6:30 AM. The diurnal fluctuations and temperature gradients have been reported for buffaloes. During summer when ambient temperature and solar radiation was maximum, adult buffaloes were not able to maintain their thermal balance even after increasing the pulmonary frequency 5 - 6 times. The changes in skin temperature at various sites indicate that the temperature of skin surface not only varies in relation to exposure but also due to water diffusion and evaporation.

• Proceedings of the KWS Conference
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• 2003.05a
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• pp.260-262
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• 2003

In this study, high temperature tensile properties of high tensile strength steels(POSTEN60, POSTEN80) were investigated by elevated temperature tensile test. According toe the results, high temperature tensile strength of POSTEN60 deteriorated slowly to 100$^{\circ}C$. As the temperature went up the tensile strength became better because of blue shortness and it deteriorated radically after reached to the maximum value around 300$^{\circ}C$. For the POSTEN80, high temperature tensile strength deteriorated slowly to 200$^{\circ}C$.As the temperature went up the tensile strength became better and it deteriorated slowly to 600$^{\circ}C$ after reached to the maximum value around 300$^{\circ}C$. Strain of high tensile strength steels at the elevated temperature increased radically after the mercury rose to 600$^{\circ}C$. The strain hardening ratio of POSTEN60 was larger then that of POSTEN80 at the elevated temperature as in the case at the room temperature and it became smaller radically after the mercury rose to 400$^{\circ}C$.

• Journal of Electrochemical Science and Technology
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• v.12 no.1
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• pp.38-57
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• 2021

The different weight ratios of Pd to Pt, i.e., 16:4, 10:10, 4:16 in Pd-Pt/C and Pd (20 wt. %) /C electrocatalysts with low metal loading were synthesized for glycerol electrooxidation in an air breathing microfluidic fuel cell (MFC). The cell performance on Pd-Pt (16:4)/C anode electrocatalyst was found best among all the electrocatalysts tested. The single cell when tested at a temperature of 35℃ using Pd-Pt (16:4)/C, showed maximum open circuit voltage (OCV) of 0.70 V and maximum power density of 2.77 mW/㎠ at a current density of 7.71 mA/㎠. The power density increased 1.45 times when cell temperature was raised from 35℃ to 75℃. The maximum OCV of 0.78 V and the maximum power density of 4.03 mW/㎠ at a current density of 10.47 mA/㎠ were observed at the temperature of 75℃. The results of CV substantiate the single cell performance for various operating parameters.

• Journal of Environmental Science International
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• v.20 no.8
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• pp.1041-1048
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• 2011

The purpose of this study was to analyze the effects on meteorological variables in Seoul, Busan and Jeju during the partial solar eclipse event of 22 July 2009 in Korea. Solar irradiance decreased 16 and 19 minutes after eclipse in Seoul and Busan, and 6 minutes before eclipse in Jeju. Minimum solar irradiance occurred 7 and 3 minutes after maximum eclipse in Seoul and Busan, respectively, and 8 minutes before maximum eclipse in Jeju. Solar irradiance began to increase after maximum eclipse in Seoul and Busan, and recovered to the original state as eclipse ended. On the other hand, recovery of solar irradiance after maximum eclipse in Jeju was slower than those of two cities. Temperature drop due to partial solar eclipse were $0.7^{\circ}C$, $4.0^{\circ}C$, $1.5^{\circ}C$ in Seoul, Busan, and Jeju, respectively, and time needed to arrive minimum temperature from maximum eclipse were each 12, 32, 30 minutes, respectively. Change of relative humidity during partial solar eclipse were 2.6%, 17.4%, 12.3% in Seoul, Busan, and Jeju, respectively. Temperature drop turned out to be sharper as altitude increases. Wind speed decreased by each about 1.1 m/s, 3.4 ms/s, 1.4 ms/s due to partial solar eclipse in Seoul, Busan, and Jeju. Soil temperature of 5 cm equally decreased by $0.2^{\circ}C$ in Seoul and Busan, soil temperature of 10 cm maintained almost constant, and soil temperature of 20 cm was hardly affected by eclipse.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.5
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• pp.561-568
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• 2003

An experiment using high preheated air in a turbulent non-premixed flame was performed to investigate the effects of high preheated air on the combustion characteristics. Combustion using high preheated and diluted air with flue gas is a new technology which enables NO emission to be reduced. In this study, Na was used as diluent and propane as fuel. Combustion characteristics, especially the distributions of the flame temperature, NO concentration and OH radical intensity were examined under the condition of 300 K, 600 K, 1000 K in terms of the combustion air temperature, and also under the condition of the dilution level from 21% to 13% in terms of oxygen concentration. As the preheated air temperature increased, it appeared that the flame length became shorter, maximum flame temperature increased, the reaction region moved to upstream, and NO concentration increased, but the flame temperature's fluctuation was reduced. In opposite, it was shown with decrement of oxygen concentration at the maximum temperature that both maximum value and the gradient of the flame temperature decreased, and NO emission also decreased considerably, but its fluctuation became larger, being inclined to be unstable.

• Progress in Superconductivity and Cryogenics
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• v.4 no.1
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• pp.78-83
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• 2002

Since the KSTAR superconducting magnet structure should be maintained at a cryogenic temperature of about 4 K, even a small amount of heat might be a major cause of the temperature rise of the structure. The Joule heating by eddy currents induced in the magnet structure during the KSTAR operation was found to be a critical parameter for designing the cooling scheme of the magnet structure as well as defining the requirements of the refrigerator for the cryogenic system. Based on the Joule heating calculation, it was revealed that the bulk temperature rise of the magnet coil structure was less than 1 K. The local maximum temperature especially at the inboard leg of the TF coil structure increased as high as about 21 K for the plasma vertical disruption scenario. For the CS coil structure, the maximum temperature was obtained from the PF fast discharging scenario. This means that the vertical disruption and PF fast discharging scenarios are the major scenarios for the design of TF and CS coil structures, respectively. For the reference scenario, the location of maximum temperature spot changes according to the transient current variation of each PF coil.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.17 no.5
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• pp.492-496
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• 2004

In this study, (0.96 －x)(PSN-PZT)－xBF－0.04 PNW＋0.3wt%MnO$_2$＋0.6wt%CuO ceramics were fabricated with the variations of the amount of BiFeO$_3$substitution and sintering temperature for the development of modified ceramics which can be sintered in the low temperature($\leq$100$0^{\circ}C$ ), and their microstructural, dielectric and piezoelectric characteristics were investigated. As the amount of BiFeO$_3$ substitution was increased, the density, mechanical quality factor(Q$_{m}$) and electromechanical coupling factor(k$_{p}$) showed the maximum value at each of sintering temperature. At sintering temperature of 98$0^{\circ}C$ and BiFeO$_3$substitution of 2 mol%, the density, dielectric constant and electromechanical coupling factor(k$_{p}$) showed the maximum value of 7.84 g/㎤, 1415 and 0.49, respectively. And at sintering temperature of 95$0^{\circ}C$ and BiFeO$_3$substitution of 3mol%, mechanical quality factor showed the maximum value of 1062. 1062.

• Journal of the Semiconductor & Display Technology
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• v.16 no.1
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• pp.16-21
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• 2017

In this paper, the effect of battery-package shape of electric vehicle on the forced convection around a group of battery cells has been numerically investigated. Simulations for the two package shapes with straight/curved ducts have been conducted to examine the two design factors; the maximum temperature and the temperature deviation of a group of cells which influence the cell durability. The simulation of the conjugate heat transfer has been simplified by employing an equivalent thermal conductivity of cell that consists of various materials. It has been found that the maximum temperature and the temperature deviation of curved duct were lower than those of straight duct. Velocity fields have also been examined to describe the temperature distribution of a group of cells and the position of maximum temperature was found to be related to the dead zone of flow field.

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.2
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• pp.259-268
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• 1998

This study was carried out to evaluate the effect of open space on temperature chang. In open space, lower temperature zones were formed, while high temperature zones were developed around urban areas. In forests and on water within open space, marked temperature reduction was recorded. The 10 % increase of the ratio of green space within fifty meters around the observation point decreased 0.33~0.43$^{\circ}C$ from the maximum temperature and 0.20~0.$25^{\circ}C$ from the minimum temperature. The increase of ten trees in the area decreased 0.33~0.49$^{\circ}C$ from the maximum temperature and 0.20~0.23$^{\circ}C$ from the minimum temperature.