• 제목/요약/키워드: Maximum dilution

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고정화 균체 반응기에서 첨가물 희석발효배지를 이용한 연속 알콜생산 (Continuous Ethanol Production Using Diluted Fermentation Media with Supplements in an Immobilized Cell Reactor)

  • 임성한;신철수
    • KSBB Journal
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    • 제10권3호
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    • pp.231-236
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    • 1995
  • Saccharomyces sake Kyokai No.7을 이용한 고정화 균체 반응기에서 연속 알콜생산시에 발효배지 성분을 1/10배 ~3/10배 범위에서 희석하여 사용할 때 균체의 생산성과 고정화 beads의 물리적 안정성이 장기적으로 유지되었다. Egg albumin hydrolysate (0.5%)와 phosphatidylcholine(0.5%)을 첨가한 발효배지를 3/10배 희석하여 공급할 때 $1.1lhr^{-1}$ 희석 율에서 최대 알콜생산성인 $69 g/\ell$-hr 이 얻어졌으며, 이는 무첨가물의 2/1011H 희석 발효배지의 최대치인 $46 g/\ell$-hr 에 비하여 50% 증가한 것이다.

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$H_2/CO/CO_2$ 합성가스화염에서 선호확산 효과에 관한 연구 (A Study on Preferential Diffusion Effects in $H_2/CO/CO_2$ Syn-gas Flames)

  • 김태권;박정;하지수
    • Journal of Advanced Marine Engineering and Technology
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    • 제32권5호
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    • pp.737-746
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    • 2008
  • Numerical study is conducted to grasp preferential diffusion effects on flame characteristics in $H_2/CO$ syn-gas diffusion flames diluted with $CO_2$. The models of Sun et al. and David et al., which have been well known to be best-fitted for $H_2/CO$ synthetic mixture flames. are evaluated for $H_2/CO$ synthetic mixture flames diluted with $CO_2$. Comparison of flame structures with mixture-averaged species diffusion and suppression of the diffusivities of $H_2$ and H was made. The behaviors of maximum flame temperatures with those species diffusion models are not explained by scalar dissipation rate but by the nature of chemical kinetics. Importantly-contributing reaction steps to heat release rate are also compared for the three species diffusion models in $H_2/CO/CO_2$ flames with and without $CO_2$ dilution.

유산균발효에 관한 동력학적연구(제4보) -연속배양에 있어서의 동력학적연구- (Kinetic Studies of Lactic Acid Fermentation(Part 4) -Kinetic Stuies on Continuous Cultivation-)

  • 이근태;양현석
    • 한국수산과학회지
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    • 제15권3호
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    • pp.179-184
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    • 1982
  • Lartobacillus bulgricus를 glucose를 제한기질로 하는 합성배지에서 연속배양한 결과를 요약하면 다음과 같다. 1. 본 실험 model은 Monod의 chemostat 이론을 적용시킬 수 있다. 2. 본 실험 model에서의 최대 cell production rate는 $0.178 g/1{\cdot}hr$로서 회석율 $0.414hr^{-1}$ 일 때이다. 3. 연속배양 결과 saturation constant($K_s$)는 7.69g/l, 최대비증식속도$(V_{max})$$0.62hr^{-1}$ 이었다. 4. wash out 현상은 $0.51hr^{-1}$에서 일어났으며cell yield coefficient는 0.016g/l 이었다.

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동축 이중 에틸렌 확산화염의 매연 농도분포 및 온도 측정 (Soot Concentration and Temperature Measurements in Laminar Ethylene Jet Double-concentric Diffusion Flames)

  • 이교우;정종수;황정호
    • 대한기계학회논문집B
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    • 제26권3호
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    • pp.402-409
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    • 2002
  • Experiments were performed with double-concentric diffusion flame(DDF) in order to investigate the characteristics of soot formation and temperature distributions. The flame size and shape of the DDF are similar to those of the well-known normal co-flow diffusion flame(WF), except the formation of a tiny inverse flame near the central tube exit. A laser light extinction technique was used to measure the soot volume fractions. The temperature distributions in the flame were measured by rapid insertion of a R-type thermocouple. Soot concentrations along the flame axis of the DDF were higher than those of the NDF. However, the maximum soot volume fraction of the DDF along the periphery of the flame was lower than that of the NDF. It is mainly due to the effect of nitrogen-dilution from the inner air. Measured temperature distribution explains these trends of soot concentration. The temperature along the flame axis was also higher in DDF than that of the NDF. However, the flame temperatures at the flame front of the two flames were almost same regardless of the inner flame. This phenomenon means that the inverse flame inside the DDF did not affect on the flame structure including the temperature and soot concentration, except the region around the flame axis.

오염총량평가를 위한 부하지속곡선 개발 및 적용 (Development of Load Duration Curve Methodology for TMDL Evaluation)

  • 강두기;강순구;김상단;신현석
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2007년도 학술발표회 논문집
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    • pp.652-656
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    • 2007
  • The major streams in South Korea have established the TMDL(Total Maximum Daily Loads) regulation for just 4 years. Traditional concepts in water quality management in South Korea are based upon the selection of a design streram flow which is 10-year averged flow exeedance probability 75%(Q275). That is, a single flow value based upon average long term flow conditions is chosen for application in dilution calculations, permit design, water quality modeling, etc. While these TMDLs seems to satisfy the requirement of the target water quality regulations, they have contributed little to any watershed/waterbody assessment and restoration plans. These types of TMDLs do little to characterize the problems the TMDLs are intended to address. For TMDLs to be more beneficial in the assessment and implementation process, TMDLs should reflect adequate water quality across flow conditions rather than at a single flow value such as average daily flow. In this paper, we developed LDC (load duration curve) methodology for theevaluation of Korean TMDL evaluation based on watershed scaled, physically based on SWAT(Soil and Water Assessment Tool) model.

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재조합 Saccharomyces cerevisiae의 Invertase 발현에 미치는 아미노산과 용존산소의 영향 (Effect of Amino Acids and Dissolved Oxygen on Expression of Invertase in Recombinant Saccharomyces cerevisiae)

  • 신해헌;조정섭;변유량;박혜영
    • 한국미생물·생명공학회지
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    • 제20권3호
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    • pp.348-354
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    • 1992
  • SUC2 유전자를 가진 재조합 Saccharomyces cerevisiae의 invertase 생산성을 향상시키기 위하여 균체 생육과 유전자 발현에 미치는 아미노산과 용존산소 농도의 영향을 연구하였다. 균체 생육과 invertase 발현에 적합한 leucine과 histidine의 최적 첨가량은 탄소원인 포도당에 대한 비율로서 나타내어 0.03g/g glucose와 0.04g/g glucose였다. 회분배양에서는 통기량이 적을수록 invertase 비활성이 증가하였다. 희석율 0.09h에서 용존산소농도를 조절한 연속배양에서는 요존산소농도가 감소함에 따라 유전자 발현이 잘되어 5 포화 이하일때 invertase 비활성이 급격히 증가하여 통기를 하지 않은 조건에서 125.54KU/g cell의 최고 비활성을 얻었다.

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Cycling Performance and Surface Chemistry of Si-Cu Anode in Ionic Liquid Battery Electrolyte Diluted with Dimethyl Carbonate

  • Nguyen, Cao Cuong;Kim, Dong-Won;Song, Seung-Wan
    • Journal of Electrochemical Science and Technology
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    • 제2권1호
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    • pp.8-13
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    • 2011
  • Interfacial compatibility between the Si-Cu electrode and diluted ionic liquid electrolyte containing 50 vol.% of 1M lithium bis(trifluoromethanesulfonyl)imide (LiTFSI)/1-methyl-1-propylpyrrolidinium bis(trifluoromethylsulfonyl)imide (MPP-TFSI) and 50 vol.% dimethyl carbonate (DMC) in a lithium cell and dilution effect on surface chemistry are examined. ex-situ ATR FTIR analysis results reveal that the surface of the Si-Cu electrode cycled in the diluted ionic liquid electrolyte is effectively passivated with the SEI layer mainly composed of carboxylate salts-containing polymeric compounds produced by the decomposition of DMC. Surface species by the decomposition of TFSI anion and MPP cation are found to be relatively in a very low concentration level. Passivation of electrode surface with the SEI species contributes to protect from further interfacial reactions and to preserve the electrode structure over 200 cycles, delivering discharge capacity of > 1670 $mAhg^{-1}$ and capacity retention of 88% of maximum discharge capacity.

Correlations between Electrical Properties and Process Parameters of Silicon Nitride Films Prepared by Low Temperature (100℃) Catalytic CVD

  • Noh, Se Myoung;Hong, Wan-Shick
    • 한국세라믹학회지
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    • 제52권3호
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    • pp.209-214
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    • 2015
  • Silicon nitride films were deposited at $100^{\circ}C$ by using the catalytic chemical vapor deposition technique. The source gas mixing ratio, $R_N=[NH_3]/[SiH_4]$, was varied from 10 to 30, and the hydrogen dilution ratio, $R_H=[H_2]/[SiH_4]$, was varied from 20 to 100. The breakdown field strength reached a maximum value at $R_N=20$ and $R_H=20$, whereas the resistivity decreased in the same sample. The relative permittivity had a positive correlation with the breakdown field strength. The capacitance-voltage threshold curve showed an asymmetric hysteresis loop, which became more squared as $R_H$ increased. The width of the hysteresis window showed a negative correlation with the slope of the transition region, implying that the combined effect of $R_N$ and $R_H$ overides the interface defects while creating charge storage sites in the bulk region.

Cultivation of Lactobacillus crispatus KLB46 Isolated from Human Vagina

  • Chang, Chung-Eun;Kim, Seung-Cheol;So, Jae-Seong;Yun, Hyun-Shik
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권2호
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    • pp.128-132
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    • 2001
  • Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a string antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63h(sup)-1 and the highest viable cell count (1.9$\times$10(sup)9 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53$\times$10(sup)9 CFU/mL) was obtained at a dilution rate of 0.32h(sup)-1, and was 7.33$\times$10(sup)11 CFU L(sup)-1 h(sup)-1, that is approximately 5 times higher than that obtained from batch culture.

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미세조류와 패류의 성장에 미치는 Triorganotin의 급성 독성영향 및 만성독성 실험을 위한 Chemostat System의 설계 (The Acute Toxicity Effect of Triotganotin on the Growth of Microalgae and Shellfish and A Design of A Chemostat System for the Chronic Toxicity Experiment)

  • 탁건태;이형호;홍용기;김중균
    • 생명과학회지
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    • 제7권4호
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    • pp.377-383
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    • 1997
  • The acute toxicity effect of triorganotin of trioganotin on the growth of microalgae and shellfish was investigated through flask culture. The value of 120 hr-LC$_{50}$ that is the median lethal concentration of TBTO on the shellfish (R. philipinarum) was found to be 6 $\mu$g/L. The acute toxicity effect of TBTO on T. suecica was obviously shown even at the concentration of 0.5 $\mu$g/L, and the effect diminished as the initial cell density increased. The effect also diminished less in the experiment done under aeration than in that done under non-aeration. To design a chemostat system for the test of chronic toxicity, the culture of T. suecica was executed in photobioreactor. In batch culture, the profiles of chlorophyII a and D.C.W. showed the growth of T. suecica very well, and the maximum specific growth rate was estimated to be 0.54 d$^{-1}$. with this value, as a dilution rate in contimuous culture, pH was nicely maintained between 7 and 9 when air was supplied with 3% CO$_{2}$. From all results and the natural environment of clam, a novel chemostat system was invented. Through this system, we can observe each independent toxicity effect of TBTO and plankton and combined toxicity effect as well.

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