• Title/Summary/Keyword: Maximum dilution

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Continuous Ethanol Production Using Diluted Fermentation Media with Supplements in an Immobilized Cell Reactor (고정화 균체 반응기에서 첨가물 희석발효배지를 이용한 연속 알콜생산)

  • 임성한;신철수
    • KSBB Journal
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    • v.10 no.3
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    • pp.231-236
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    • 1995
  • For continuous ethanol production In an immobilized cell reactor consisting of Saccharomyces sake, feedings of one tenth to three tenths times diluted fermentation media were effective for maintaining the high ethanol productivity and physical stability of immobilized beads. In case two tenths times dilltued one of the fermentation medium supplemented with egg albumin hydrolysate(0.5%) and phosphatidylcholine(0.5%) was fed, a maximum ethanol productivity of $69 g/\ell$-hr was attained at a dilution rate of$1.1lhr^{-1}$, and it was 50% higher than that of the two tenths times diluted one of the fermentation medium without any supplement, $46 g/\ell$-hr.

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A Study on Preferential Diffusion Effects in $H_2/CO/CO_2$ Syn-gas Flames ($H_2/CO/CO_2$ 합성가스화염에서 선호확산 효과에 관한 연구)

  • Kim, Tae-Kwon;Park, Jeong;Ha, Ji-Soo
    • Journal of Advanced Marine Engineering and Technology
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    • v.32 no.5
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    • pp.737-746
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    • 2008
  • Numerical study is conducted to grasp preferential diffusion effects on flame characteristics in $H_2/CO$ syn-gas diffusion flames diluted with $CO_2$. The models of Sun et al. and David et al., which have been well known to be best-fitted for $H_2/CO$ synthetic mixture flames. are evaluated for $H_2/CO$ synthetic mixture flames diluted with $CO_2$. Comparison of flame structures with mixture-averaged species diffusion and suppression of the diffusivities of $H_2$ and H was made. The behaviors of maximum flame temperatures with those species diffusion models are not explained by scalar dissipation rate but by the nature of chemical kinetics. Importantly-contributing reaction steps to heat release rate are also compared for the three species diffusion models in $H_2/CO/CO_2$ flames with and without $CO_2$ dilution.

Kinetic Studies of Lactic Acid Fermentation(Part 4) -Kinetic Stuies on Continuous Cultivation- (유산균발효에 관한 동력학적연구(제4보) -연속배양에 있어서의 동력학적연구-)

  • LEE Keun-Tai;YANG Hyeun-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.179-184
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    • 1982
  • The behavior of continuous flow culture of Lartobacillus bulgricus was investigated by application of Monod's kinetic model. The parameters obtained from Monod's chemostat theory successfully predicted the behavior of the chemostat. Then, it was found that Monod's kinetics were applicable to the growth rate dependence on glucose concentration. Under steady-state condition, the maximum growth rate, saturation constant, and wash out were found to be 0.62/hr, 7.69 g/1, 0.51/hr of continuous culture. And the optimum condition for the highest cell production was 0.41/hr in dilution rate, and at that point the cell production rate was 0.178g/1 hr.

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Soot Concentration and Temperature Measurements in Laminar Ethylene Jet Double-concentric Diffusion Flames (동축 이중 에틸렌 확산화염의 매연 농도분포 및 온도 측정)

  • Lee, Gyo-U;Jeong, Jong-Su;Hwang, Jeong-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.26 no.3
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    • pp.402-409
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    • 2002
  • Experiments were performed with double-concentric diffusion flame(DDF) in order to investigate the characteristics of soot formation and temperature distributions. The flame size and shape of the DDF are similar to those of the well-known normal co-flow diffusion flame(WF), except the formation of a tiny inverse flame near the central tube exit. A laser light extinction technique was used to measure the soot volume fractions. The temperature distributions in the flame were measured by rapid insertion of a R-type thermocouple. Soot concentrations along the flame axis of the DDF were higher than those of the NDF. However, the maximum soot volume fraction of the DDF along the periphery of the flame was lower than that of the NDF. It is mainly due to the effect of nitrogen-dilution from the inner air. Measured temperature distribution explains these trends of soot concentration. The temperature along the flame axis was also higher in DDF than that of the NDF. However, the flame temperatures at the flame front of the two flames were almost same regardless of the inner flame. This phenomenon means that the inverse flame inside the DDF did not affect on the flame structure including the temperature and soot concentration, except the region around the flame axis.

Development of Load Duration Curve Methodology for TMDL Evaluation (오염총량평가를 위한 부하지속곡선 개발 및 적용)

  • Kang, Du-Kee;Kang, Soon-Ku;Kim, Sang-Dan;Shin, Hyun-Suk
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.652-656
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    • 2007
  • The major streams in South Korea have established the TMDL(Total Maximum Daily Loads) regulation for just 4 years. Traditional concepts in water quality management in South Korea are based upon the selection of a design streram flow which is 10-year averged flow exeedance probability 75%(Q275). That is, a single flow value based upon average long term flow conditions is chosen for application in dilution calculations, permit design, water quality modeling, etc. While these TMDLs seems to satisfy the requirement of the target water quality regulations, they have contributed little to any watershed/waterbody assessment and restoration plans. These types of TMDLs do little to characterize the problems the TMDLs are intended to address. For TMDLs to be more beneficial in the assessment and implementation process, TMDLs should reflect adequate water quality across flow conditions rather than at a single flow value such as average daily flow. In this paper, we developed LDC (load duration curve) methodology for theevaluation of Korean TMDL evaluation based on watershed scaled, physically based on SWAT(Soil and Water Assessment Tool) model.

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Effect of Amino Acids and Dissolved Oxygen on Expression of Invertase in Recombinant Saccharomyces cerevisiae (재조합 Saccharomyces cerevisiae의 Invertase 발현에 미치는 아미노산과 용존산소의 영향)

  • 신해헌;조정섭;변유량;박혜영
    • Microbiology and Biotechnology Letters
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    • v.20 no.3
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    • pp.348-354
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    • 1992
  • In order to improve the productivity of invertase by recombinant Saccharomyces cerevisiae containing SUC2 gene, the effect of amino acids and dissolved oxygen concentration on the gene expression was investigated. Optimal concentrations of leucine and histidine for cell growth and cloned gene expression were 0.03 gig and 0.04 gig, respectively, expressed as the ratio of amino acid/glucose. The lack or excess of leucine and histidine has inhibitory effect on cell growth and invertase expression. In batch culture, the less aeration was, the higher invertase activity was. In continuous culture at a dilution rate of 0.09 h 1 with controlled dissolved oxygen tension, invertase activity increased dramatically at DOT levels below 5% air saturation, and a maximum activity of 215.54 KUlg cell was obtained under unaerated condition.

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Cycling Performance and Surface Chemistry of Si-Cu Anode in Ionic Liquid Battery Electrolyte Diluted with Dimethyl Carbonate

  • Nguyen, Cao Cuong;Kim, Dong-Won;Song, Seung-Wan
    • Journal of Electrochemical Science and Technology
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    • v.2 no.1
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    • pp.8-13
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    • 2011
  • Interfacial compatibility between the Si-Cu electrode and diluted ionic liquid electrolyte containing 50 vol.% of 1M lithium bis(trifluoromethanesulfonyl)imide (LiTFSI)/1-methyl-1-propylpyrrolidinium bis(trifluoromethylsulfonyl)imide (MPP-TFSI) and 50 vol.% dimethyl carbonate (DMC) in a lithium cell and dilution effect on surface chemistry are examined. ex-situ ATR FTIR analysis results reveal that the surface of the Si-Cu electrode cycled in the diluted ionic liquid electrolyte is effectively passivated with the SEI layer mainly composed of carboxylate salts-containing polymeric compounds produced by the decomposition of DMC. Surface species by the decomposition of TFSI anion and MPP cation are found to be relatively in a very low concentration level. Passivation of electrode surface with the SEI species contributes to protect from further interfacial reactions and to preserve the electrode structure over 200 cycles, delivering discharge capacity of > 1670 $mAhg^{-1}$ and capacity retention of 88% of maximum discharge capacity.

Correlations between Electrical Properties and Process Parameters of Silicon Nitride Films Prepared by Low Temperature (100℃) Catalytic CVD

  • Noh, Se Myoung;Hong, Wan-Shick
    • Journal of the Korean Ceramic Society
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    • v.52 no.3
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    • pp.209-214
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    • 2015
  • Silicon nitride films were deposited at $100^{\circ}C$ by using the catalytic chemical vapor deposition technique. The source gas mixing ratio, $R_N=[NH_3]/[SiH_4]$, was varied from 10 to 30, and the hydrogen dilution ratio, $R_H=[H_2]/[SiH_4]$, was varied from 20 to 100. The breakdown field strength reached a maximum value at $R_N=20$ and $R_H=20$, whereas the resistivity decreased in the same sample. The relative permittivity had a positive correlation with the breakdown field strength. The capacitance-voltage threshold curve showed an asymmetric hysteresis loop, which became more squared as $R_H$ increased. The width of the hysteresis window showed a negative correlation with the slope of the transition region, implying that the combined effect of $R_N$ and $R_H$ overides the interface defects while creating charge storage sites in the bulk region.

Cultivation of Lactobacillus crispatus KLB46 Isolated from Human Vagina

  • Chang, Chung-Eun;Kim, Seung-Cheol;So, Jae-Seong;Yun, Hyun-Shik
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.2
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    • pp.128-132
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    • 2001
  • Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a string antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63h(sup)-1 and the highest viable cell count (1.9$\times$10(sup)9 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53$\times$10(sup)9 CFU/mL) was obtained at a dilution rate of 0.32h(sup)-1, and was 7.33$\times$10(sup)11 CFU L(sup)-1 h(sup)-1, that is approximately 5 times higher than that obtained from batch culture.

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The Acute Toxicity Effect of Triotganotin on the Growth of Microalgae and Shellfish and A Design of A Chemostat System for the Chronic Toxicity Experiment (미세조류와 패류의 성장에 미치는 Triorganotin의 급성 독성영향 및 만성독성 실험을 위한 Chemostat System의 설계)

  • Tak, Keon-Tae;Lee, Hyong-Ho;Hong, Yoog-Ki;Kim, Joong Kyun
    • Journal of Life Science
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    • v.7 no.4
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    • pp.377-383
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    • 1997
  • The acute toxicity effect of triorganotin of trioganotin on the growth of microalgae and shellfish was investigated through flask culture. The value of 120 hr-LC$_{50}$ that is the median lethal concentration of TBTO on the shellfish (R. philipinarum) was found to be 6 $\mu$g/L. The acute toxicity effect of TBTO on T. suecica was obviously shown even at the concentration of 0.5 $\mu$g/L, and the effect diminished as the initial cell density increased. The effect also diminished less in the experiment done under aeration than in that done under non-aeration. To design a chemostat system for the test of chronic toxicity, the culture of T. suecica was executed in photobioreactor. In batch culture, the profiles of chlorophyII a and D.C.W. showed the growth of T. suecica very well, and the maximum specific growth rate was estimated to be 0.54 d$^{-1}$. with this value, as a dilution rate in contimuous culture, pH was nicely maintained between 7 and 9 when air was supplied with 3% CO$_{2}$. From all results and the natural environment of clam, a novel chemostat system was invented. Through this system, we can observe each independent toxicity effect of TBTO and plankton and combined toxicity effect as well.

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