• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.163-169
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• 2001

OSBA(oocytes-sperm binding assay) is a tool developed for rapid test of optimal condition of IVF medium and protein source by binding ability of mouse sperm and egg. Mouse oocyte-cumulus complexes were prepared by removing of the cumulus cells with 0.1% hyaluronidase. 10$\pm$2 oocytes per 30 ${mu}ell$ medium drop were inseminated with 3 ${mu}ell$ sperm suspension and were cultured f3r 3 hours and 24 hours, respectively. And the oocytes were recovered gently and the No. of sperm bound on oocytes were counted. In the Exp. 1, the ratio of oocytes bound with one sperm at least were 60.2%(50/83), 2%(2/77) and 100%(79/79) in the medium with no protein, FBS(15%, v/v) and BSA(0.4%. w/v), respectively, Fetal bovine serum(FBS) seriously inhibited sperm binding on oocyte, although bovine serum albumin(BSA) promoted the binding ability. The inhibiting effect of FBS was dependent on the concentration of FBS. The sperm binding ability according to oocyte maturity was tested in the Exp. 2. There was no significant difference between Met. II (mature) and Met. I (intermediate mature) oocytes in the number of oocytes bound with sperm and the number of sperm bound on oocytes. Finally, in Exp. 3, two batches of Ham's F10 medium with good and poor quality by OSBA were tested (The ratios of embryos developed from PN 1-cell stage to hatched blastocyst; 25% vs. 70%). In the medium with good quality, sperm binding ability was significantly increased (P ＜ 0.05). The ratio of oocytes bound with one sperm at least was 66% and 90% in the medium with poor and good quality, respectively. Conclusively, It was possible to test IVF medium condition rapidly and easily by OSBA.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.2
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• pp.201-207
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• 1987

This experiment was conducted to identify the superior corn hybrids for silage production and to determine the combining ability of their parental inbred lines. A 7-entry diallel cross was evaluated for grain and stover yields, and agronomic characters under 3 different seasons. Hybrids Hi34 x Tx601, Hi26 x Hi34, and Hi29 x Hi34 showed high total dry matter yields. In general, late maturing hybrids had higher grain and silage yields than early maturing hybrids. Leaf area index was correlated with grain and stover yields. Rust rating was negatively correlated with yields and all characters, indicating that rust (Puccinia sorghi) was one of the major factors affecting yields. Diallel analysis showed that inbred Hi34 which was late in maturity and resistant to rust was the best combiner for both grain and silage yields. General combining ability (GCA) and specific com-bining ability (SCA) effects for yields and other characters were significant, suggesting that both additive and non-additive gene effects are involved for those characters. Ratio of GCA/SCA mean squares showed that GCA effects were more important than SCA effects for yields and other characters. GCA x season and SCA x season interactions were significant for grain and stover yields, revealing that gene effects were not stable for those yields under dissimilar environments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.3
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• pp.187-194
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• 2010

The soybean stay green mutant genotype (SSG) derived from the nuclear gene, d1d2, and cytoplasmic gene, cytG, inhibit the breakdown of chloroplast in the leaves, pod walls, seed coats, and embryos during maturity. Soybean seed with black seed coat and green cotyledon (SBG) are preferred than black seed coat with yellow cotyledon (SBY) especially for cooking with rice and as source of traditional food in Korea. The researchers evaluated the seed's chlorophyll content of SSG and introduced SSG to the SBG variety breeding program. The seed chlorophyll content of SSG with d1d2 was $39.93{\sim}60.80\;{\mu}g/g$ and SSG with cytG $38.08{\sim}39.89\;{\mu}g/g$. The Korean SBG variety which was derived from SSG with cytG, contains $16.35{\sim}37.73\;{\mu}g/g$. The composition of seed chlorophyll differs according to the genetic background of SSG genotype. Inheritance study showed that cotyledon color was segregated 15:1 (yellow:green) at $F_2$ seed indicating two recessive genes control green cotyledon as revealed by previous study. Only less than 3% soybean lines showed black seed coat with green cotyledon among crosses SBY and SSG (d1d2). Results showed that SSG with d1d2 can be used as a good source for SBG with high chlorophyll content in the seed cotyledon, but due to the complex genetic behavior, breeding resource of SBG with d1d2 should be prepared to improve the breeding efficiency for development SBG variety.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.1
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• pp.83-90
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• 2010

Crop characteristics of 179 sorghum (Sorghum bicolor L. Moench) resources collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Spike types of 179 sorghum resources were classified as 5 types of open-loose type, broom-tillering type, half broom-tillering type, extreme-compact type and compact type, of which broom-tillering type was the highest ratio of 38.0% (68 plant resources) of 179 germplasm. In the existence and nonexistence of spike awn in 179 sorghum resources, 28.5% (51 plant resources) showed existence of spike awn, whereas the rest of 71.5% (128 plant resources) had no spike awn. Seed type was classified as 5 types, of which circle-shape showed the highest ratio of 43.0% (77 plant resources) and the lowest was inclined-circle shape by 7.3% (13 plant resources). Seed color was classified as 4 colors of brown, white, whitish brown, and yellowish brown, of which yellowish brown was the highest ratio of 84.4% (151 plant resources) among them. Days from seeding to heading date showed the range from 67 to 88 days with the average of 77.4 days and the highest frequency proportion of it was the group from 76 to 80 days, which occupied 37.4% (67 plant resources) of 179 germplasm. 179 sorghum resources showed high variation in the range of culm length with the average of 253.8 cm and group from 92 cm to 360 cm of culm length showed the highest frequency proportion of 20.6% (37 plant resources). Spike length showed the range from 15 to 49 cm with the average of 28.8 cm and the highest frequency distribution of it was the group from 31 to 35 cm with the proportion of 26.3% (47 plant resources), whereas the lowest was below 15 cm with the proportion of 0.6%. Days from seeding to physiological maturity showed the range from 110 to 146 days with the average of 125.5 days and the highest frequency proportion (39.7%) of it was the group from 111 to 115 days, while there were also occupied with 2 plant resources below 110 days and 23 plant resources over 141 days among 179 germplasm. Number of grains per spike showed the range from 163 to 4,532 grains with the average of 2,068.6 grains and group from 1,601 to 2,000 grains per spike was the highest frequency distribution with the proportion of 25.7% (46 plant resources). 1000 grains weight showed the range from 10.6 to 38.1g with the average of 25.6g and group from 26.0 to 30.0g was the highest frequency distribution with the proportion of 44.1% (79 plant resources).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.164-173
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• 2018

Elevated atmospheric carbon dioxide concentration ($CO_2$) is a major component of climate change, and this increase can be expected to continue into the crop and food security in the future. In this study, Soil-Plant-Atmosphere-Research (SPAR) chambers were used to examine the effect of elevated $CO_2$, temperature, and drought on the canopy architecture and concentration of macronutrients in potatoes (Solanum tuberosum L.). Drought stress treatments were imposed on potato plants 40 days after emergence. Under AT+2.8C700 (30-year average temperature + $2.8^{\circ}C$ at $700{\mu}mol\;mol^{-1}$ of $CO_2$), at maximum leaf area, elevated $CO_2$, and no drought stress, a significant increase was observed in both the aboveground biomass and tuber, and for the developmental stage. Even though $CO_2$ and temperature had increased, AT+2.8C700DS (30-year average temperature + $2.8^{\circ}C$ at $700{\mu}mol\;mol^{-1}$ of $CO_2$ under drought stress) under drought stress showed that the leaf area index (LAI) and dry weight were reduced by drought stress. At maturity, potatoes grown under $CO_2$ enrichment and no drought stress exhibited significantly lower concentrations of N and P in their leaves, and of N, P, and K in tubers under AT+2.8C700. In contrast, elevated $CO_2$ and drought stress tended to increase the tuber Mg concentration under AT+2.8C700DS. Plants grown in AT+2.8C700 had lower protein contents than plants grown under ATC450 (30-year average temperature at $400{\mu}mol\;mol^{-1}$ of $CO_2$). However, plants grown under AT+2.8C700 showed higher tuber bulking than those grown under AT+2.8C700DS. These findings suggest that the increase in $CO_2$ concentrations and drought events in the future are likely to decrease the macronutrients and protein concentrations in potatoes, which are important for the human diet.

• Economic and Environmental Geology
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• v.51 no.4
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• pp.381-392
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• 2018

This study examines strategies and implementation plans for commercializing $CO_2$ capture and storage, which is an effective method to achieve the national goal of reducing greenhouse gas. In order to secure cost-efficient business model of $CO_2$ capture and storage, we propose four key strategies, including 1) urgent need to select a large-scale storage site and to estimate realistic storage capacity, 2) minimization of source-to-sink distance, 3) cost-effectiveness through technology innovation, and 4) policy implementation to secure public interest and to encourage private sector participation. Based on these strategies, the implementation plans must be designed for enabling $CO_2$ capture and storage to be commercialized until 2030. It is desirable to make those plans in which large-scale demonstration and subsequent commercial projects share a single storage site. In addition, the plans must be able to deliver step-wised targets and assessment processes to decide if the project will move to the next stage or not. The main target of stage 1 (2019 ~ 2021) is that the large-scale storage site will be selected and post-combustion capture technology will be upgraded and commercialized. The site selection, which is prerequisite to forward to the next stage, will be made through exploratory drilling and investigation for candidate sites. The commercial-scale applicability of the capture technology must be ensured at this stage. Stage 2 (2022 ~ 2025) aims design and construction of facility and infrastructure for successful large-scale demonstration (million tons of $CO_2$ per year), i.e., large-scale $CO_2$ capture, transportation, and storage. Based on the achievement of the demonstration project and the maturity of carbon market at the end of stage 2, it is necessary to decide whether to enter commercialization of $CO_2$ capture and storage. If the commercialization project is decided, it will be possible to capture and storage 4 million tons of $CO_2$ per year by the private sector in stage 3 (2026 ~ 2030). The existing facility, infrastructure, and capture plant will be upgraded and supplemented, which allows the commercialization project to be cost-effective.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.310-317
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• 2014

This study was conducted to find basic information for the research of morphological dormancy in seeds of two genera (Heloniopsis and Thalictrum) native to Korea. Seeds of Heloniopsis koreana, Heloniopsis tubiflora, Thalictrum rochenbrunianum, Thalictrum uchiyamai, and Thalictrum coreanum were collected, and embryo morphology and seed germination were investigated. All seeds of five species had underdeveloped embryos which occupied about 10-20% of the full seed length at maturity. The seeds of H. koreana and H. tubiflora had rudimentary embryos. The seeds of T. rochenbrunianum, T. uchiyamai, and T. coreanum had intermediate type between rudimentary and linear embryo. The small embryos of all seeds of five species grew to a critical length before radicles emerge from the seeds. At 30 days after sowing, the seeds of H. koreana and H. tubiflora germinated to 22.7% and 40.7%, respectively. On the other hand, the seeds of T. rochenbrunianum, T. uchiyamai, and T. coreanum germinated to 12.5%, 3.3%, and 0%, respectively. If embryo growth and germination in seeds are completed in approximately 30 days, the seed are said to have morphological dormancy (MD). However, if the seeds require > 30 days for germination, they are considered to have morphophysiological dormancy (MPD). Thus, the seeds of H. koreana and H. tubiflora had about 78% and 60% MPD, respectively. On the other hand, the seeds of T. rochenbrunianum, T. uchiyamai, amd T. coreanum had about 87%, 96%, and 100% MPD, respectively. There was a different level of dormancy (MD and MPD) within the same seed population examined. Therefore, germination of the seeds was delayed due to the underdeveloped embryos. This result could contribute to determine morphological seed dormancy and germination mechanism in seeds of the Korean Peninsula.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.959-965
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• 2016

In 1994, a new cultivar 'Joyskin' was created from a cross between the cultivars 'Whangkeumbae' and 'Waseaka' at the Pear Research Institute of the National Institute of Horticultural and Herbal Science, Rural Development Administration. In 2006, the 'Joyskin' was selected from among the 317 seedlings resulting from the cross for its skin and taste qualities. Regional adaptation tests were conducted in nine regions and in ten experimental plots from 2006 to 2011. The cultivar was named in 2011. 'Joyskin' showed a vigorous growth habit and semi-spread characteristics similar to 'Whangkeumbae'. The average full bloom date for 'Joyskin' was April 21st, which was also similar to 'Whangkeumbae'. The optimum fruit ripening time was September 6-8th, which was six or eight days earlier than 'Whangkeumbae'. The fruit was round in shape and the skin was a golden yellow color at maturity. The average fruit weight was 320 g and the flesh firmness was $2.5kg/8mm{\varphi}$. The firmness of the fruit skin determined by a blade-type plunger of texture analyzer was 22.9 N, which was significantly different from that of 'Whangkeumbae' 29.9N. Stone cell analysis of 'Joyskin' by phloroglucinol-HCl, showed that 'Joyskin' stone cells were small in size and few in numbers cpmpared to those of cultivars of was 'Manpungbae', 'Niitaka', and 'Whangkeumbae'. The patent application for 'Joyskin' was submitted in April, 2012 (Grant No. 2012-337). In 2016, 'Joyskin' (Grant No. 5895) was registered as a separate record, with uniformity and stability per Korean Seed Industry Law.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.1
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• pp.7-16
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• 2005

This experiments were carried out to determine growth characteristics, dry matter yield and voluntary intake of silage according to growth stage of Sorghum$\times$sudangrass(SSH) hybrid in mono-cropping and inter-cropping(C; com, T1; cutting of SSH at milk stage. T2; cutting of SSH at dough stage, T3; cutting of SSH at yellow ripe stage, T4; cutting of SSH and soybean at milk stage, T5; cutting of SSH and soybean at dough stage, T6: cutting of SSH and soybean at yellow ripe stage). Results obtained from these experiments are as follows; Plant length and leaf length of SSH(T2, T3, T4. T5 and T6)) were higher than C. T1 treatment was lower than it. C showed $2.3\~2.9$ times higher stem diameter as 29.5mm compare to SSH(T1, T2, T3, T4, T5 and T6). soybean(T4, T5 and T6) was lower in $4.3\~5.4$ times. But SSH of inter-cropping treatment(T4, T5 and T6) showed highly comparing with mono-cropping(T1, T2 and T3) at the same maturity. Leaf rate and stem hardness of SSH(T1, T2, T3, T4, T5 and T6) were lower than C. The fresh yield was high line with T2(72,320kg/ha), T1(69,103kg/ha), T3(68,333kg/ha) and C(57,988kg/ha), dry matter yield was high in line with T3(22.413kg/ha), T2(21,479kg/ha), C(19,252kg/ha) and T6(18,175kg/ha), (P<0.05). Protein dry matter yield was higher in T3(1,434kg/ha), C(1,386kg/ha)T5 and T6(1,345kg/ha) it was lower in T1(872kg/ha), (P<0.05). Crude protein of silage of T4 and T5 was higher than C, T2 and T3 were lower than it(P<0.05), while NDF content was not different. ADF content of T6 was higher than those of the other treatment. The highest hemi-cellulose among treatments was shown in T1 whereas T6 showed the lowest. Fresh intake of silge was 160.4, 155.8, 168.7, 172.9, 132.9, 158.7 and 185.2 g/BW for C, T1, T2, T3, T4, T5 and T6, respectively. Dry matter intake was high in line with T6(60.3g), C(153.8g), T3(53.6g), T5(47.8g), T2(46.8g), T4(35.2g) and T1(34.48g/BW), (P<0.05). Crude protein intake was high in line with T6(3.9g), T5(3.4g), C(3.4g), T2(2.9g), T3(2.9g), T4(2.6g) and T1(2.3g/BW), (P<0.05). As mentioned above the results, mono-cropping(T3) and inter-cropping(T5 and T6) could be recommended as increasing method of sorghum $\times$ sudangrass hybrid silage utilization when silage intake of dry matter and crude protein were considered.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.198-206
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• 1995

Baled silage making(BS) of selected forage crops was discussed during 1991-1993, to determine the best cutting time of the plants for BS production, BS yields and silage quality. Seven species of forage crops and pasture grasses(rye, barley, spring oat, Italian ryegrass, orchardgrass, alfalfa and grass-legume pasture mixtures) were harvested at different stage of growth from young plant to physiological maturity, and baled in a self constructed square baling chamber. Each bales, measured 90cm length, 60cm width and 50cm height, were wrapped with 0.05mrn thick polyethylene plastic film, and stored in stack silo. Each bales were weighed between 15-20 kg in dry matter basis. The effects of pre wilting and formic acid addition on the silage quality of young plant materials, which contained high water concentration, was also evaluated during the experiment. Rye plant including of barley and spring oat were evaluated as a good materials for baled silage making. Fodder rye produced high quality BS with a value of silage quality point 84(Flieg's point) when the plant harvested at stage of greatest dry matter accumulation by 12.64 tonha. The best quality BS of barley was obtained at stage of hard dough to yellow stage by 11.9 ton/ha dry matter yield and 81 point silage quality. Italian ryegrass and pasture grasses including of orchardgrass, alfalfa and grass-legume pasture mixtures procuced also high quality bale silage by harvesting at stage of late blooming. However pre witting operation and formic acid addition was required for BS production of grass materials because of high water contents. Water contents of Italian ryegrass and other pasture species ranged 18.9%(Italian) to 20.8%(alfalfa). Silage quality point of Italian BS harvested at late blooing was increased from 72 to 88 by 1/2-one day pre wilting and 0.3% formic acid treatment. Silage quality of young plant materials of rye and other forage crops, barley and spring oat were also improved markedly through the pre wilting treatment and formic acid addition.