• Journal of Radiation Protection and Research
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• v.28 no.1
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• pp.9-18
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• 2003

Rice plants at different seed-developing stages were exposed to HTO vapor in an exposure box for 1 h during daytime and nighttime to investigate the levels of tissue free water $^3H$ (TFWT) and organically bound $^3H$ (OBT) in different plant parts. In the daytime experiment, TFWT concentrations in leaves at the end of exposure $(h_0)$ were around 100% of the 1 hour mean HTO concentrations in air moisture whereas in the nighttime experiment, they were as low as $30{\sim}40%$ of the air concentration. TFWT concentrations in both experiments decreased very rapidly in the beginning but much mote slowly later and those at harvest were hundreds to hundred thousands times lower than those at $h_0$. OBT concentrations varied with time in different manners depending on plant parts and exposure times and differed between at $h_0$ and at harvest by factors of less than 10 on the whole. Even during nighttime exposures, OBT was produced at about a third the rate for daytime exposures. The degree of the conversion of airborne HTO into OBT in mature rice seeds, being several times higher in the daytime experiment than in the nighttime experiment, was highest after the exposure peformed at the most actively seed-developing stage for both experiments. It is estimated that OBT would contribute much more to the ingestion radiation dose than TFWT if rice plants are exposed to HTO vapor for the seed-developing period.

• Journal of Life Science
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• v.29 no.7
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• pp.774-778
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• 2019

Soybean [Glycine max (L.) Merr.] is one of the major food sources of protein, oil, carbohydrates, isoflavones, and other nutrients for both humans and animals. However, soybean seeds contain antinutritional factors, such as lectin protein, Kunitz Trypsin Inhibitor (KTI) protein, and stachyose. The objective of this research was to stack recessive alleles for development a triple recessive genotype, titilelers2rs2, with low KTI protein, lectin protein, and stachyose contents. Three parents (Gaechuck#2, PI200508, and 14G20) were used to develop the breeding population. The presence or absence of the lectin and KTI proteins was detected by western blotting. The stachyose content in mature seeds was determined by HPLC. Agronomic traits, such as plant type, plant height, maturity date, lodging, seed quality, and 100-seed weight, were evaluated for the four $F_3$ plant strains. One $F_4$ plant strain with the desired agronomical traits was selected. One new strain with the triple recessive titilelers2rs2 genotype was developed. The plant height of the new strain was 51 cm and the 100-seed weight was 31.0 g. The new strain had a yellow seed coat and yellow hilum. The stachyose content of the new strain was 3.8 g/kg. One strain developed in this research will be used to produce improved yellow soybean cultivars that are free of lectin and KTI proteins and low in stachyose content.

• Journal of Life Science
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• v.34 no.5
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• pp.313-319
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• 2024

Cultivars or genetic resources with a black seed coat and green cotyledons are rich in lutein, which can promote eye health, and anthocyanin, known for its numerous health benefits. However, mature seeds also contain P34, 7S α' subunit, and lectin proteins, which are allergenic and degrade quality. Here, we report the breeding of a new soybean line with a black seed coat, green cotyledon, and free of P34, 7S α' subunit, and lectin proteins. A total of 157 F₂ seeds with black seed coats and green cotyledons were selected by crossing a female parent with a brown seed coat, green cotyledon, and lacking the 7S α' subunit and lectin proteins with a male parent with a black seed coat, green cotyledon, and lacking the P34 and lectin proteins. The P34 and 7S α' subunit proteins were consistent with a ratio of 9:3:3:1, indicating that they are independent of each other. From 14 F₂ seeds that were recessive (cgy1cgy1p34p34) for both proteins, one individual F₂ plant (F₃ seeds) with the desired traits-black seed coat, green cotyledon, and lacking P34, 7S α' subunit, and lectin proteins- was finally selected. The triple null genotype (absence for P34, 7S α' subunit, and lectin proteins) was confirmed in random F₃ seeds. The selected line has a black seed coat and green cotyledons, and when sown on June 14 in the greenhouse, the maturity date was approximately October 3, the height was about 68 cm, and the 100-seed weight was about 26.5 g.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.86-92
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• 1992

Green buckwheat seedlings have been used as a pollution free vegetable and medicinal crop for a long time. Some of the reasons are the rapid growth rate, the high protein and rutincontent, and a more favorable ratio of leaf to stem than mature plants. Off-crop season cultivation techniques were developed for growing the young and green buckwheat vegetable of higher quality and yield, and for its increased value-added income. The effects of planting season, seeding rate, seed bed soil fertility and type, and seedling growth period on yield and rutin content were determined for vegetable and medicinal use. The young vegetable yields. in the off season culture ranged from 2.62t/ha to 22.7t /ha. The highest vegetable yield was 22.7t /ha for 25 days old seedlings grown in the polyethylene film tunnel from March 30 to April 25, 1991 where seedling rate was 360kg /ha. Buckwheat vegetable quality and income were dependent upon planting season, seeding rate, growing duration and temperature, and facilities of raising seedlings. Protein content of buck-wheat seedlings was from 21.5% to 17.2%. Rutin content of the vegetable was 53.9～31.7mg /100g for the whole plant in average. The protein and rutin content was significantly varied due to the different environmental conditions including fertility and type of seed bed soil, growing duration, temperature of the green house, and polyethylene film house and tunnel.

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.225-234
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• 1997

This study was carried out to investigate the native distribution of wax myrtle (Myrica rubra) in Cheju Province and its propagation method. Wax myrtle is heavily distributed at 100 to 400m above sea level of Donhong-chun and Hyodon-chun which is bordered by Youngchon-dong of Seogwipo City and Haryeri of Namcheju county. BA, IBA, Kinetin, IAA, and GA3 were applied to promote rooting of cutting but were ineffective in promoting rooting. Treating cuttings with both AgNO,1 which removes rooting inhibitor, tannin and growth regulators such as BA, IBA and Kinetin were also ineffective in promoting rooting. Incubating seeds at 4$0^{\circ}C$ for 30 days resulted in 39％ germination. There was distinct difference in leaf shape between seedlings and mature trees.

• Korean journal of applied entomology
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• v.18 no.1 s.38
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• pp.29-33
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• 1979

An attempt was made to find out whether barley stripe mosaic virus (BSMV) occurs in barley and wheat cultivars in Korea. Modified Hamilton's technique for serological detection of barley stripe mosaic virus was used to assay for BSMV in mature seed embryos. Of the 51 barley and wheat seed lots assayed, BSMV was detected in 34 of them. BSMV was detected in covered, naked, and matting barley as well as in wheat. The level of BSMV infection in the infected cultivars varied from $2\~35\%$. This is the first reported occurrence of barley stripe mosaic virus in Korea.

• Korean Journal of Plant Resources
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• v.22 no.3
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• pp.209-214
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• 2009

To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. The embryo in vitro rescue was performed to increase the recovery of backcross progenies. The $F_{2}$ generation was more amenable than $F_{1}$ hybrids to produce backcross progenies. The $F_{1}$ hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum) (recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BC$F_{1}$ ${\times}$ F. esculentum (thrum) and F. homotropicum ${\times}$ BC$F_{1}$ was the disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat was used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses and inter-crosses between $F_{1}$ plants and selected fertile plants of the second congruity backcrosses.

• Journal of Life Science
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• v.14 no.4
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• pp.644-649
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• 2004

During plant development, active gibberellins (GAs) control many aspects of plant growth and development including seed germination, stem elongation, flower induction, anther development and seed growth. To understand the biosynthesis and functional role of active GAs in high plants, this study investigated GA 3$\beta$-hydroxylase gene en-coding $GA_1$ and$GA_4$ catalizing last step in GA biosynthetic pathway. The antisense GA 3$\beta$-hydroxylase gene was inserted into expression vector, pIG121-Hm. Calli derived from mature seeds of rice (Oryza satiiva L. cv. Donjinbyeo) were co-cultivated with Agrohacterium tumefaciens EHA101 earring a pIG121-Hm containing hygromycin resistance ($Hyg^r$) and antisense GA 3$\beta$-hydroxylase gene. Seventeen transgenic plants obtained inhibiting GA 3$\beta$-hydroxylase. Transgenic plants had shorter plant height more than that of the Dongjinbyeo. Stable integration of antisense GA 3$\beta$-hydroxylase gene was confirmed by polymerase chain reaction of genomic DNA isolated from the leaf organs of the $T_o$ generation.

• Molecules and Cells
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• v.19 no.1
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• pp.67-73
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• 2005

Isoflavones are synthesized by isoflavone synthases via the phenylpropanoid pathway in legumes. We have cloned two isoflavone synthase genes, IFS1 and IFS2, from a total of 18 soybean cultivars. The amino acid residues of the proteins that differed between cultivars were dispersed over the entire coding region. However, amino acid sequence variation did not occur in conserved domains such as the ERR triad region, except that one conserved amino acid was changed in the IFS2 protein of the GS12 cultivar ($R_{374}G$) and the IFS1 proteins of the 99M06 and Soja99s65 cultivars ($A_{109}T$, $F_{105}I$). In three cultivars (99M06, 99M116, and Simheukpi), most of amino acid changes were such that the difference between the amino acid sequences of IFS1 and IFS2 was reduced. The expression profiles of three enzymes that convert naringenin to the isoflavone, genistein, chalcone isomerase (CHI), isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) were examined. In general, IFS mRNA was more abundant in etiolated seedlings than mature plants whereas the levels of CHI and F3H mRNAs were similar in the two stages. During seed development, IFS was expressed a little later than CHI and F3H but expression of these three genes was barely detectable, if at all, during later seed hardening. In addition, we found that the levels of CHI, F3H, and IFS mRNAs were under circadian control. We also showed that IFS was induced by wounding and by application of methyl jasmonate to etiolated soybean seedlings.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.647-651
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• 1997

Flowering date of peanut variety, Daekwangtangkong and Daepungtangkong in non-mulching culture was delayed each 9 and 6 days. Seedling ratio was decreased each 3.7%, 16% and branch length was decreased each 24%, 17% more than polyethylene film mulching culture. But the number of branch in non-mulching culture was not reduced. No. of mature pods, kernel/pod ratio, 100-seed weight and kernel weight per plant was reduced extremely in non-mulching culture, nevertheless the kernel yield per 1 ha of Daekwangtangkong in non-mulching culture was similar to that of polyethylene film mulching culture but yield of Daepungtangkong was 26% higher than that of polyethylene film mulching culture owing to planting density. Protein content of Daekwangtangkong in non-mulching culture was increased 4% but that of Daepungtangkong was decreased 3% and oil content of both variety was decreased 7%, 9% respectively comparing to polyethylene film mulching culture. Oleic acid was increased but linoleic acid was decreased and O/L ratio was decreased in non-mulching culture.