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http://dx.doi.org/10.5352/JLS.2004.14.4.644

Antisense GA 3β-Hydroxylase Gene Transferred to Rice Plants.  

강용원 (경북대학교 농업생명과학대학 식물생명과학부)
윤용휘 (경북대학교 농업생명과학대학 식물생명과학)
김길웅 (경북대학교 농업생명과학대학 식물생명과학)
이인중 (경북대학교 농업생명과학대학 식물생명과학)
신동현 (경북대학교 농업생명과학대학 식물생명과학부)
Publication Information
Journal of Life Science / v.14, no.4, 2004 , pp. 644-649 More about this Journal
Abstract
During plant development, active gibberellins (GAs) control many aspects of plant growth and development including seed germination, stem elongation, flower induction, anther development and seed growth. To understand the biosynthesis and functional role of active GAs in high plants, this study investigated GA 3$\beta$-hydroxylase gene en-coding $GA_1$ and$GA_4$ catalizing last step in GA biosynthetic pathway. The antisense GA 3$\beta$-hydroxylase gene was inserted into expression vector, pIG121-Hm. Calli derived from mature seeds of rice (Oryza satiiva L. cv. Donjinbyeo) were co-cultivated with Agrohacterium tumefaciens EHA101 earring a pIG121-Hm containing hygromycin resistance ($Hyg^r$) and antisense GA 3$\beta$-hydroxylase gene. Seventeen transgenic plants obtained inhibiting GA 3$\beta$-hydroxylase. Transgenic plants had shorter plant height more than that of the Dongjinbyeo. Stable integration of antisense GA 3$\beta$-hydroxylase gene was confirmed by polymerase chain reaction of genomic DNA isolated from the leaf organs of the $T_o$ generation.
Keywords
Antisense GA 3$\beta$-hydroxylase; Polymerase chain reaction; Rice (Oryza sativa L.); Transgenic plants; $T_o$ generation;
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