• 한국작물학회지
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• 제51권6호
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• pp.534-538
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• 2006

The effects of Rhizobium inoculant, compost, and nitrogen on nodulation, growth, dry matter production, yield attributes, and yield of pea (Pisum sativum) var, IPSA Motorshuti-3 were assessed by a field experiment. Among the treatments Rhizobium inoculant alone performed best in recording number and dry weight of nodules/plant. The highest green seed yield of 8.38 ton/ha (36.9% increase over control) and mature seed yield of 2.97 ton/ha (73.7% increase over control) were obtained by the application of 90 kg N/ha. The effects of 60 kg N/ha, Rhizobium inoculant alone and Rhizobium inoculant along with 5 ton compost/ha were same as the effect of 90 kg N/ha in recording plant height, root length, dry weight of shoot, and root both at preflowering and pod filling stages, number of mature pods/plant, number of mature seeds/pod, 1000-seed weight, green, and mature seed yields of pea.

• 한국자원식물학회지
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• 제28권3호
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• pp.350-356
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• 2015

Abeliophyllum distichum is a monotypic taxon of Oleaceae and endemic to Korea. A comprehensive study on embryogeny and fruit and seed coat ontogeny in Abeliophyllum was carried out via microtome and light microscopy. The fertilization occurs during mid– to late April and embryo matures by early July. The embryo development follows the general fashion from globular embryo – transition embryo – heart shaped embryo – torpedo embryo – walking-stick embryo to mature embryo. The pericarp clearly differentiates into three histological zones: exocarp, mesocarp, and endocarp. The young seed comprises 10-12 cells thick seed coat and the mature seed coat comprises an exotesta, 6-8 mesotesta and an endotesta. Any crystals, phenolic-like compounds, idioblasts, and the sclereids are not found in pericarp as well as seed coat. An overall development confirms Solanade type of embryogenesis in Abeliophyllum. The endocarp becomes more prominent in mature fruit and all the layers of endocarp are highly lignified. On the basis of mechanical layer the seed coat is of exotestal type.

• Journal of Ecology and Environment
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• 제42권3호
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• pp.90-102
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• 2018

Background: We sought to understand the relationship between the seed bank and vegetation in abandoned rice paddies in South Korea, in order to guide management of these sites. We investigated the floristic composition and species richness of the soil seed bank and ground vegetation in former paddies along three seral gradients (wet meadow, young forest, and mature forest) in Gwangneung Forest Biosphere Reserve. Results: Seed bank samples contained 59 species, of which the dominant families were Cyperaceae, Gramineae, and Polygonaceae. Species richness and seedling density (59 taxa and 19,121 germinants from all samples) were high. Carex spp. (11, 921 germinants) were the dominant taxa. The species composition in the seed bank changed gradually as the land transitioned from wet meadow to mature forest. Sørensen's index of similarity between above- and below-ground vegetation was 29.3% for wet meadow, 10.8% for young forest, and 2.1% for mature forest. Germinant density also declined, with 10, 256 germinants for wet meadow, 6445 germinants for young forest, and 2420 germinants for mature forest. Conclusions: Changes in aboveground environment and life history traits such as amphicarpic plants, likely affect the composition of soil seed bank species. Abandoned paddy fields may be good sites for restoration of wetland forest and conservation of wetland habitat. Some intervention may be required to promote the recovery of a natural species assemblage.

• 한국작물학회지
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• 제48권3호
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• pp.152-155
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• 2003

야생의 안동대목은 종자의 크기 및 충실도에 많은 변이를 보이고 있다. 본 연구는 약용 및 박과접목묘의 대목용으로 이용되고 있는 안동대목 종자의 크기 및 성숙 정도가 발아, 유묘 출현 및 생장에 미치는 영향을 조사하기 위하여 실시되었던 바 그 결과를 요약하면 다음과 같다. 안동대목 종자의 발아율 및 유묘출현율은 미성숙 종자에 비하여 성숙 종자에서 높았다. 그러나 종자의 크기에 따른 발아율은 성숙 종자와 미성숙 종자 모두 대립종자에 비하여 중립 또는 소립종자에서 높았던 반면, 유묘출현율은 중립종자에서 가장 높고 대립종자에서 가장 낮았다. 종자 크기와 성숙 정도가 유묘 형태 및 생장에 미치는 영향으로는 하배축 길이를 제외한 초장, 본엽수, 자엽 및 본엽의 면적은 종자 크기가 클수록 길고 많았던 반면, 종자의 성숙 정도간에는 차이가 없었다. 그러나 각 부위 및 전체 건물중은 미성숙 대립종자보다는 성숙 대립종자에서, 성숙 유무에 관계없이 종자의 크기가 클수록 많았다

• 한국자원식물학회지
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• 제9권2호
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• pp.113-119
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• 1996

This study inverstigates growing seedling of onion for 91 farmhouses at Muan, Hampyeong and Sinan which are major areas of production from Jan. to Apr., 1994 to enhance the research effect of onion and then the following results are obtained. Over 60% of the farmhouses have cultured onion continuously over 11years. Early mature variety cultured is over 9 varieties and among them Kinkyu variety is cultured most frequently. Late mature variety is over 13 and Changyongdaego, Bonganwhang and Chunjudaego varieties are cultured most. Most of the farmhouses used seeds less than 6 dl at small area less than $40m^2$ and especially in the case of early variety ,they purchased it at seed stores with 15,000-20,000 won/dl and sowed them with broadcasting method. Amount of fertilizer level of onion seed is ranged from nonfertilizer level to $16.8g/m^ and most of the farmhouses employ molding after seeding with straw and compost and they did not use thinning. Irrigation is performed by 1-4 times before sprouting of onion seed and most of the farmhouses transplant long nursery plant over 46 days hand weeding is used.

• 생명과학회지
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• 제11권4호
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• pp.311-315
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• 2001

To identify the effects embryo developmental stage and gelrite concentration on plant regeneration in seed culture of rice, mature and immature seeds of rice were cultured on the $N_{6}$ medium supplemented with2 mg/$\ell$ 2.4-D and different levels of gelrite(0.2~1.0%). The calli formed immature embryos were produced more plants than those from mature embryos. The maximum frequency of plant regeneration was achieved in the culture of the calli of immature embryos which was harvested at the 21$^{th}$ day after pollination. The plant regeneration on the medium with gelrite was more accelerate than that on the medium with agar. The highest frequency(55%) of plant regeneration was obtained from the calli transferred to the medium with 6g/$\ell$ gelrite.

• Journal of Plant Biology
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• 제32권1호
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• pp.11-21
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• 1989

Anatomical and histochemical changes taking place in Piper nigrum berries during their ripening are described. The important observations on the pericarp are the development of sclereids in the exocarp, a continuous band of oil cells in mesocarp and the wall thickening of the endocarpic cells. The mature seed with a single layer of seed coat, representing the innermost tegment layer, encloses abundant perisperm. The endosperm and embryo are situated laterally at the terminal part of the seed. The perisperm is distinguished into an outer protein-rich zone and inner starch-filled zone. Starch and protein are also deposited in the mature pericarpic tissue. Lipid bodies are seem in the form of oil globules in oil cells.

• Journal of Plant Biotechnology
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• 제47권2호
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• pp.118-123
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• 2020

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F₂ seeds were obtained. A 3:1 segregation ratio was observed during F₂ seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F₂ seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ²=2.87, P=0.5 - 0.1). From nine F₂ seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

• 원예과학기술지
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• 제34권6호
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• pp.830-839
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• 2016

Germination of Hemerocallis seeds as influenced by cold stratification at $5^{\circ}C$ (CS) and maturity of seeds evaluated using X-ray imaging has not been well investigated. Seeds of H. lilioasphodelus, H. citrina, H. citrina 'April Flower', and H. minor collected from China and H. thunbergii collected from Korea were germinated at $20^{\circ}C$ without pre-temperature treatment, while H. hongdoensis, H. dumortieri, H. minor, and H. vespertina seeds were treated with CS. Harvesting 'Stella de Oro' capsules at 35-40 days after anthesis yielded mature seeds with well-developed embryo and cotyledons analyzed by X-ray images with a 92% germination in 17 days after sowing. Seeds of H. thunbergii and H. citrina germinated in less than 13 days without CS; two weeks of CS did not accelerate seed germination. Seeds of H. hongdoensis germinated in 24 days when seeds were stored at $25^{\circ}C$ without CS and in less than 27 days when cold stratified. Therefore, 'Stella de Oro' capsules should be harvested at 35-40 days after anthesis to harvest mature seeds. Cold stratification is not required to accelerate seed germination in the Hemerocallis taxa evaluated in this study.

• Applied Biological Chemistry
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• 제32권3호
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• pp.222-231
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• 1989

대두에 존재하는 단백질 분해 효소 저해제로는 Kunitz trypsin inhibitor (SKTI), Bowman-Birk proteinase inhibitor, 그리고 그의 isoinhibitor들이 알려져 있다. SKTI는 분자량이 20K이며 181개의 아미노산으로 구성되어 있다. SKTI의 분자 구조 및 발현 특이성을 밝히기 위하여, 콩에서 순수 분리한 SKTI를 항원으로 사용하여 항체를 제조하였다. 이 항체는 항원으로 사용한 SKTI에 대하여 특이적으로 반응할 뿐만 아니라 기내에서 합성된 그의 전구체와도 특이적으로 반응하였다. 기내에서의 단백질 합성은 미성숙 대두 종자에서 mRNA를 분리한 후, wheat germ extract를 이용하여 실시하였다. 합성된 단백질 중에서 대두에 존재하는 SKTI는 검출되지 않는 반면에 면역침전법에 의해 분자량이 24K인 전구체가 존재하고 있음을 확인하였다. 이와 같이 SKTI는 mRNA로부터 전구체가 합성된 후 post-translational modification에 의해 완전한 SKTI로 변환됨을 알 수 있었다. 또한 SKTI는 대두 종자의 성숙과 함께 발현이 되는 것으로 보아 조직 및 분화 특이성 발현형태를 나타내는 것을 알 수 있었다.