• 제목/요약/키워드: Mature leaf

검색결과 157건 처리시간 0.024초

Leaf-specific pathogenesis-related 10 homolog, PgPR-10.3, shows in silico binding affinity with several biologically important molecules

  • Han, Jin Haeng;Lee, Jin Hee;Lee, Ok Ran
    • Journal of Ginseng Research
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    • 제39권4호
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    • pp.406-413
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    • 2015
  • Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

Kalanchoe속의 기관 부위별 표피구조와 기공유형 (Epidermal Structure and Stomatal Types in Various Parts of Each Organ of Kalanchoe)

  • 정우규
    • Journal of Plant Biology
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    • 제30권2호
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    • pp.79-94
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    • 1987
  • This study was carried out to investigate the epidermal structure, the stomatal types, the ontogeny of stomara in various parts of each organ of K. blossfeldiana, K. kewensis, and K. tometosa belonging to Kalanchoe. The epidermal cells were polygonal or isodiametric ones in the leaves, and mostly rectangular, tetragonal, and elongated ones in the leaves, and mostly rectangular, tetragonal, and elongated ones in the other organs. The candelabrum-like, triradiate stellete trichomes in the aerial parts of all organs of K. tomentosa were found. The cuticular striations and square crystals of calcium oxalate in the epidermal cells of petals of K. blossfeldiana were observed. The great majority of the mature stomata in various parts of all the organs were commonly helicocytic types. This type was subdivided into three subtypes such as parahelicocytic, anomohelicocytic, and dianisocytic stomata on the basis of the division angle of the guard mother cells. Somethies, the anisocytic type was found in most organs. This type was subdivided into three subtyes such as paranisocytic, nomoanisocytic, and dianisocytic stomata in the same way as the helicocytic type. A new stomataltype with anisocytic stoma within a girdle of four subsidiary cells of tetracytic type in the leaf of K. kewensis was firstly observed in the vascular plants. This type was termed the coaniso-tetracytic type. The anomomeristic pattern in the mesogenous category of stomatal types was found in various organs of all the material plants. Developmental mode of stomata was constant in all the parts of each organ within the same plant. The stomata was observed to be a few similar stomatal types in various parts of each organ within the same plant. The ontogeny of all the types is eumesogenous or mesogenous type. The ontogenetic type of stomata was mostly helico-eumesogenous type in all the organs of all the material plants. The mature stoma varied from organ to in regard of the number and arrangement of subsidiary cells.

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티모시의 품종에 따른 성숙종자 유래의 캘러스로부터 식물체 재분화 (Plant Regeneration from Seed Derived Callus of four Cultivars of Timothy)

  • 이기원;최기준;김기용;지희정;정민웅;김경희;이병현;이상훈
    • 한국초지조사료학회지
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    • 제31권3호
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    • pp.211-216
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    • 2011
  • 티모시의 4가지 품종을 이용하여 성숙종자로 부터 캘러스 유도 및 식물체 재분화 효율을 조사하였다. 4주 동안 배양한 종자로부터 캘러스 유도율은 Colt, Chair, Richmond, Hokuo 순으로 캘러스 유도율을 보였고, 식물체로의 재분화 또한 캘러스 유도율과 같은 Colt > Chair > Richmond > Hokuo 순으로 나타났다. 기본 배지의 종류에 따른 배양 효율의 차이는 품종별 차이는 있으나 MS 배지가, 식물체 재분화는 N6 배지와 MS 배지가 SH 배지에 비해 효과적이었다. 전체적으로는 캘러스 유도율이 높은 품종이 식물체 재분화율도 높은 경향을 나타내었다. 본 연구를 통하여 밝혀진 캘러스 유도와 식물체 재분화율이 우수한 Colt 품종은 유용유전자 도입을 통한 신품종 개발에 유용하게 이용될 수 있을 것이다.

울릉도 마가목의 다양한 부위의 항혈전 활성 (In-vitro Antithrombosis Activity of Different Parts of Sorbus commixta from Ulleung Island)

  • 김미선;성화정;손호용
    • 생명과학회지
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    • 제26권3호
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    • pp.289-295
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    • 2016
  • 마가목은 울릉도를 대표하는 약용식물 중 하나이다. 본 연구에서는 마가목을 이용한 혈행개선 기능성을 가진 고부가가치 음료 제조를 위해 마가목의 미숙과, 성숙과, 줄기 및 잎의 열수 추출물을 제조하고, 이를 대상으로 기존에 보고된 바 없는 항혈전 활성 및 항산화 활성을 검토하였다. 마가목 줄기의 경우 추출효율은 3.99%, pH 5.5이었으며, 열수 추출물은 총 폴리페놀 89.2 mg/g, 총 플라보노이드 28.3 mg/g의 함량을 나타내었다. 다양한 부위의 마가목 추출물들은 0.5 mg/ml 농도까지 인간 적혈구 용혈활성을 나타나지 않았다. 혈액응고 저해활성 평가결과, 줄기 추출물에서만 강력한 트롬빈, 프로트롬빈, 혈액응고인자 저해가 확인되었으며, 인간 혈소판 응집저해활성은 인정되지 않았다. 한편 혈전 생성억제와 관련된 항산화 활성 평가 결과, 줄기 추출물에서 강력한 DPPH 음이온, ABTS 음이온 소거능, nitrite 소거능을 확인하였으며, 각각의 RC50는 각각 119.7, 53.3, 및 117.5 μg/ml으로 계산되었다. 항산화제인 vitamin C의 DPPH 음이온, ABTS 양이온 및 nitrite 소거능에 대한 RC50가, 각각 13.7, 5.2, 및 14.9 μg/ml임을 고려할 때, 마가목 줄기 추출물은 정제되지 않은 상태에서도 우수한 항산화 활성을 나타내었으며, 환원력 역시 다른 부위에 비해 강력하였다. 본 연구결과는 강력한 항응고 활성과 항산화 활성을 나타내는 마가목 줄기 추출물이 신규의 항혈전 천연물 소재로 이용가능 함을 제시하고 있다.

종자(種子) 및 배지상(培地上)에서의 오이류(類) 덩굴마름병균의 형태적(形態的) 및 배양적(培養的) 특징(特徵) (Morphological and Cultural Characters of Didymella bryoniae on Seeds and Culture Media)

  • 이두형
    • 한국균학회지
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    • 제10권1호
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    • pp.7-13
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    • 1982
  • 자연상태(自然狀態)에서 Didymella bryoniae에 이병(罹病)된 오이 및 호박 종자(種子)를 습지법(濕紙法)으로 처리(處理)하여 불완전(不完全) 세대(世代)와 완전세대(完全世代)의 특징(特徵)을 조사(調査)하고 Difco PDA, V-8쥬스한천(寒天) 및 water agar leaf medium에서의 특징(特徵)과 비교(比較) 하였다. 병든 종자(種子)가 발아(發芽)할 때 종피(種皮)나 배축(胚軸)에 형성(形成)된 Didymella bryoniae의 병포자(柄胞子)와 Difco PDA, V-8 juice한천(寒天) 및 WALM에 형성(形成)된 본균(本菌)의 병포자(柄胞子)는 형태적(形態的)으로 많은 차이(差異)가 나타난다. 즉 PDA에 형성(形成)되는 병포자(柄胞子)는 무색(無色) 소형단포자(小型單胞子)가 대부분이며, V-8 juice한천(寒天) 및 WALM에 형성(形成)되는 것은 대형단포자외(大型單胞子外)에 격막(隔膜)이 하나 있는 포자(胞子)를 형성하고 또 균주(菌株)에 따라서는 격막(隔膜)이 두개 있는 포자(胞子)를 형성하므로써 마치 병든 종피(種皮)나 배축(胚軸)에 형성(形成)된 것과 흡사하였다. 한편 자양각은 병든 종자(種子)의 표본(標本)에 따라 잘 형성(形成)되는 것도 있었으나 대부분(大部分)의 표본(標本)에서는 형성(形成)되지 않았다. 외부형태적(外部形態的)인 특징(特徵)은 색깔이 진한 흑색(黑色)이며 유두상각공(乳頭狀殼孔) 위에 백색(白色)의 포자괴(胞子塊)가 형성(形成)되는 특징(特徵)을 가지고 있어서 쉽게 병자각(柄子殼)과 구별(區別)할 수 있었다. 불완전(不完全) 세대(世代)인 병자각(柄子殼)이나 병포자(柄胞子)와 같이 자양각의 각방(殼房)과 포자(胞子)의 크기에는 큰 변이(變異)가 없었다.

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NIS quality analysis of pre- and post-harvest sugarcane.

  • Johnson, Sarah E.;Berding, Nils
    • 한국근적외분광분석학회:학술대회논문집
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    • 한국근적외분광분석학회 2001년도 NIR-2001
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    • pp.1621-1621
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    • 2001
  • The quality of sugarcane grown on the NE Australian tropical coast ($16^{\circ}$15'- $18^{\circ}$15' S Lat.) has declined markedly in the past seven years. This has been linked to dilution of mill-supply cane with increasing levels of non mature-stalk material consisting of leaves and sucker culms. The prime research objective was to examine the transition from the pre-harvest, in-field crop to harvested material sent for processing, in terms of quality and crop fraction proportions. A secondary objective was to quantify the effects of preharvest-season crop habit and culm condition on crop quality. Ten quadrat samples from each of 54 random crop sites (17 in 1999 and 37 in 2000), covering a wide range of variables (cultivar, crop class, and edaphic, topographic, climatic, and temporal factors) were collected immediately before harvest. Samples were partitioned into four fractions:- sound and unsound mature stalks (culms), sucker culms, and extraneous matter (leaves). Material harvested from each site was sampled and partitioned into four fractions:- sound and unsound billets (culm pieces), culm-spindle pieces, and leaf. In 2000, before harvest, 14 additional sites were sampled monthly, on three occasions, from March - June. Erect and non-erect culms were divided into sound and unsound classes. All samples were disintegrated and presented to a remote reflectance module of a scanning spectrophotometer using the BSES large cassette module. Near infra-red spectroscopic (NIS) analyses were developed for the rapid determination of quality components (Brix, commercial cane sugar (CCS), fibre, moisture, and polariscope reading). Calibrations for three material groups (culm (n = 639), non-culm (n = 496), and combined) were developed for all components using the 1999 data set. Two sub-sets (n = 178, and 190) of about 10% of the preharvest-season and harvest populations scanned in 2000 also were subjected to full routine laboratory analyses. The 1999 combined calibrations were excellent, but the culm calibrations produced consistently lower standard errors. Non-culm calibrations were marginally better than the combined for only CCS and pol. reading. Analysis of the 2000 culm data with calibrations using all 1999 and 2000 culm data resulted in better predictions relative to the 1999 culm calibrations. This also was true for the combined calibrations. Assessment of quality components in pre- and post-harvest sugarcane using NIS calibrations was more cost effective than using routine laboratory techniques. Outcomes from this NIS-facilitated research will have important economic consequences for the Australian sugarcane industry. Potential CCS present in mature culms is being discounted by dilution with leaves and sucker culms, threatening farm viability. The results question the efficacy of current harvesting technology. The CCS of harvested cane is improved only marginally over that of the in-field crop. Current harvesting technology requires either supplementary, innovative pre-mill processing or a design revolution to improve mill-supply cane quality, and therefore whole of industry economics. NIS-facilitated analyses, before the harvest season, highlighted the benefits of growing erect, sound crops. Loss of CCS then, can be minimized only by a combination of crop improvement and agronomic solutions, applied as part of sound on-farm management.

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Acer okamotoanum Nakai Leaf Extract Inhibits Adipogenesis Via Suppressing Expression of PPAR γ and C/EBP α in 3T3-L1 Cells

  • Kim, Eun-Joo;Kang, Min-jae;Seo, Yong Bae;Nam, Soo-Wan;Kim, Gun-Do
    • Journal of Microbiology and Biotechnology
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    • 제28권10호
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    • pp.1645-1653
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    • 2018
  • The genus Acer contains several species with various bioactivities including antioxidant, antitumor and anti-inflammatory properties. However, Acer okamotoanum Nakai, one species within this genus has not been fully studied yet. Therefore, in this study, we investigated the anti-adipogenic activities of leaf extract from A. okamotoanum Nakai (LEAO) on 3T3-L1 preadipocytes. Adipogenesis is one of the cell differentiation processes, which converts preadipocytes into mature adipocytes. Nowadays, inhibition of adipogenesis is considered as an effective strategy in the field of anti-obesity research. In this study, we observed that LEAO decreased the accumulation of lipid droplets during adipogenesis and down-regulated the expression of key adipogenic transcription factors such as peroxisome proliferator-activated receptor ${\gamma}$ (PPAR ${\gamma}$) and CCAAT/enhancer binding protein ${\alpha}$ (C/EBP ${\alpha}$). In addition, LEAO inactivated PI3K/Akt signaling and its downstream factors that promote adipogenesis by inducing the expression of PPAR ${\gamma}$. LEAO also activated ${\beta}$-catenin signaling, which prevents the adipogenic program by suppressing the expression of PPAR ${\gamma}$. Therefore, we found that treatment with LEAO is effective for attenuating adipogenesis in 3T3-L1 cells. Consequently, these findings suggest that LEAO has the potential to be used as a therapeutic agent for preventing obesity.

Suppression of the ER-Localized AAA ATPase NgCDC48 Inhibits Tobacco Growth and Development

  • Bae, Hansol;Choi, Soo Min;Yang, Seong Wook;Pai, Hyun-Sook;Kim, Woo Taek
    • Molecules and Cells
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    • 제28권1호
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    • pp.57-65
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    • 2009
  • CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are implicated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83% of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.

구기자혹응애 (Eriophyes kuko KISHIDA) 및 그 혹(Gall)에 관한 연구. IV. 혹의 성장에 따르는 광현적(光顯的) 및 전현적(電顯的) 관찰 (Studies on Eriophyes kuko KISHIDA and its Galls. IV. Studies on the Growing Mite Gall under Light and Electron Microscopes)

  • 김창효;천송중신;소인영
    • Applied Microscopy
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    • 제2권1호
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    • pp.17-31
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    • 1972
  • 우기자혹응애 (Eriophyes kuko KISHIDA)의 기생에 의하여 구기자 나무 (Lycium Chinens MILL) 잎에 형성되는 흑 (mite gall) 의 행태적 변화를 광학현미경 및 전자현미경으로 관찰한 결과는 다음과 같다. 1. 광현적 관찰 : 구기자혹응애가 구기자나무의 어린잎의 뒷면 표피세포에 침입되면 먼지 표피세포가 이상비대를 하면서 혹이 형성되기 시작한다. 혹이 성장함에 따라 붕상조직과 해면상조직의 세포도 이상비대를 하게되고, 점차적으로 각 조직의 세포는 이상비대로 인한 불규칙한 형태적 변화를 일으켜 조직의 구별이 힘들게 된다. 이상비대한 혹세포의 핵은 건전세포의 핵보다 크며, 혹이 성장함에 따라서 핵의 크기도 커진다. 2. 전현적 관찰 : 혹이 성장함에 따라 혹세포의 mitochondria, golgi-apparatus. plastid 등은 퇴 화 및 붕괴되고, 특히 큰 흑의 이상비대한 혹세포에는 성상체 (star body) 와 환상 (ring form) 의 구조등이 나타난다.

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고려인삼, 미국삼 및 죽절삼의 생육 및 형태적 특성 비교 (Comparison of Plant Growth and Morphological Characteristics Among the Korean Ginseng, the American Ginseng and the Bamboo Ginseng)

  • 정열영;이명구
    • Journal of Ginseng Research
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    • 제22권2호
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    • pp.147-153
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    • 1998
  • An investigation was conducted to ascertain the basic information on characteristics of growth and morphological characters among the Korean (Panax. ginseng), the American (Panax. quinquefolium) and the Bamboo (Panax. japonicus) ginseng. In aerial parts growth of the ginseng species by age, The Korean ginseng and American ginseng's stem and leaf growth was alike in 2-4 years old, but growth cycle changed in 6 years old. The Korean ginseng was more vigorous than the American ginseng. The Korean ginseng roots were highly observed in ratio of red skin roots among three species, whereas The American ginseng roots were highly infected by root rot. It seems to be variable depending on growing stage and species. The Korean ginseng flowered about the middle of May, the American ginseng early June, and the Bamboo ginseng was late of May, The berry color of the ginseng species was observed, The Korean and American ginseng's mature berry color was red, The Bamboo ginseng's berry was three type of color and shape. In root characteristics of the seedling, Korean (p. ginseng), American (p. quinquefolium) ginseng's root shape was similarity in type, the bamboo ginseng showed different type, which root length and root weight was smaller than those of ginseng. In morphological characters of Leaf surface, pollen, and stoma, the Korean ginseng and American ginseng had crystal rosette on epidermis cell, but the Bamboo ginseng didn't has crystal rosette. Pollen shape observed tricolpate pollen and size was media type among the ginseng species, and also guard cell was anomocytic type, which were observed by scanning electronic microscope.

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