• Asian-Australasian Journal of Animal Sciences
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• v.28 no.2
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• pp.188-199
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• 2015

Nineteen tanniferous browse plants were collected from South Africa to investigate their digestibility, gas production (GP) characteristics and methane production. Fresh samples were collected, dried in forced oven, and ground and analyzed for nutrient composition. In vitro GP and in vitro organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure GP by incubating the sample in a shaking incubator at $39^{\circ}C$. There was significant (p<0.05) variation in chemical composition of studied browses. Crude protein (CP) content of the species ranged from 86.9 to 305.0 g/kg dry matter (DM). The neutral detergent fiber (NDF) ranged from 292.8 to 517.5 g/kg DM while acid detergent fiber (ADF) ranged from 273.3 to 495.1 g/kg DM. The ash, ether extract, non-fibrous carbohydrate, neutral detergent insoluble nitrogen, and acid detergent insoluble nitrogen and CP were negatively correlated with methane production. Methane production was positively correlated with NDF, ADF, cellulose and hemi-cellulose. Tannin decreased GP, IVOMD, total volatile fatty acid and methane production. The observed low methanogenic potential and substantial ammonia generation of some of the browses might be potentially useful as rumen manipulating agents. However, a systematic evaluation is needed to determine optimum levels of supplementation in a mixed diet in order to attain a maximal depressing effect on enteric $CH_4$ production with a minimal detrimental effect on rumen fermentation of poor quality roughage based diet.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.3
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• pp.235-240
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• 1991

A CRD experiment with thirty growing cross bred calves were assigned at random to three treatments rations. 1) $T_0$, 0% Urea + 20% M. O. cake, 2) $T_1$, 1% Urea + 10% M. O. cake and 3) $T_2$, 2% Urea + 0% M. O. cake to develop a rice straw based ration for ruminants. Sweetish odour and yellowish colour were observed in good recovered silage. Organic matter varied from 87.45% to 89.63% whereas crude protein varied from 14.0% to 14.5% in each treatment. No significant differences were found among the nutrient composition of the ration. The dry matter in take (DMI) and dry matter digestibility was higher in $T_0$ (0% Urea) than those of ration containing 1% ($T_1$) and 2% Urea ($T_2$). The organic matter digestibility decreases with increasing doses of urea. The crude protein & nitrogen-free-extract digestibility were found higher in the ration $T_1$ containing 1% urea whereas crude fibre digestibility and available metabolizable energy (ME) were higher in $T_0$ containing no urea as compared to $T_1$ and $T_2$. Total digestible nutrient (TDN) decreases with the increase of urea level. The highest feed efficiency was found in $T_0$ having no urea and lowest was in $T_2$. The animals gained in weights from each ration. Highest gain in weight was found in $T_0$ ration, then followed $T_1$, and $T_2$. This is due to natural protein available in M. O. cake only. It is concluded that supplemetation of urea or M. O. cake with readily available energy source as molasses upto 20% of total dietary dry matter in a complete ration may increase the intake of low quality fibrous roughage only when nitrogen and mineral are not limiting factor.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1297-1302
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• 2003

To determine whether the feeding of different species of hay affects the dry-matter intake and the serum cortisol level of sheep, 6 non-pregnant, non-lactating ewes were offered alfalfa (Medicago sativa) hay (Al), oats (Avena sativa) hay (Ot) and perennial ryegrass (Lolium perenne) hay (Pr) under 6 kinds of treatment including 1) change from Al to Ot, 2) Al to Pr, 3) Ot to Al, 4) Ot to Pr, 5) Pr to Al, and 6) Pr to Ot. The experimental design was a 6${\times}$6 Latin square with a 14 day period of which 9 days were a preliminary period and 5 days as a digestion trial period. The change in hay feeding was done abruptly on the first day of each period without an adaptation period. The blood from the jugular vein was collected on the first, third and the last day of each period through a sterile catheter for the analysis of cortisol. The dry-matter intake was recorded daily throughout the period. The mean daily intake of dry matter (DM, g/kg live weight) was significantly different among the 3 species of hay (Al>Ot>Pr; p<0.05). The digestibility of DM for Al and Ot was the same, but that for Pr was significantly lower than Al and Ot (p<0.05). The mean serum cortisol levels were significantly different among the hays (p<0.05). The level for Pr was the highest and that for Al was the lowest. The abrupt change of hay feeding of 6 treatments produced a significant change in the serum cortisol levels. The DM intake was inversely related to the change of the cortisol level. It is suggested that the animal' intake response to different species of hay may be partly motivated by the psychological feelings toward the hay offered.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.2
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• pp.122-126
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• 1983

The allocation of the dry-matter yield in Miscanthus sineusis grasslands on soils of different water contents was investigated. The results obtained in this study can be summarized as follows; 1. The standing crops of Miscanthus sineusis grassland A (soil water content; 22.54%) and B (soil water content; 9.22%) were 2,267.12 and 943.44 g/m$^{2}$, respectively. 2. The productivity curves of the Miscanthus sineusis grassland A and B were the bell-shape and the productivity peak values were showed in September. 3. In Miscanthus sineusis grassland A and B, the reproductive allocation was represented by characteristics of production structure and dry-matter yields. 4. Allocation rates of dry-matter yields to panicle and rhizomes in Miscanthus sineusis grassland A and B were 0.6 and 1.4% and 35.4 and 46.6% respectively. 5. In these results, allocation of dry-matter yield to reproductive organs was increased in the condition of water-stress.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.4
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• pp.583-590
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• 1989

Increasing levels of ammonia-N in the rumen fluid used for in vitro incubation were achieved by supplementation of the ration of the donor cows with urea and by addition of urea either with or without glucose to the rumen fluid after collection. The ration of the donor animals consisted of wheat straw (80%) and maize silage (20%). During the second half of the experiment the basal ration was supplemented with a mineral mixture. Wheat straw, Guinea grass and two rice straw varieties were incubated with the various kinds of rumen fluid. Parameters studied were: solubility, apparent organic matter disappearance after 48 hours of incubation ($OMD_{48}$), rate of organic matter degradation from 0 to 24 hours of incubation ($k_1$) and from 24 to 95 hours ($k_2$). The concentration of ammonia-N in the rumen fluid at which 95% of the maximal $OMD_{48}$ and k1 were reached (88.2 and 100.0 mg/l) were independent of the feed. With regard to the $k_2$ the required ammonia-N concentration to reach 95% of the maximal $k_2$ differed per feed. Mineral supplementation increased the OMD48 and $k_1$, but not the solubility and $k_2$. Glucose addition in combination with urea had no beneficial effect compared to urea supplementation alone.

• Journal of Microbiology
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• v.42 no.1
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• pp.60-63
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• 2004

A sucrose-agar globule (SAG) was newly introduced to increase production of the vesicular arbuscular mycorrhizal (VAM) fungal spores, Gigaspora gigantea and Glomus fasciculatum. An SAG inoculum and a sucrose-agar globule with root exudates (SAGE) inoculum were prepared, and their spore productions were compared with a soil inoculum. When the SAGE was used as the inoculum on sucrose-agar medium plates the number of spores was increased (35% more than the soil inoculum). After the soil inoculum and SAGE were inoculated on an experimental plant, Zingiber officinale, the percentage root colonization, number of VAM spores, and dry matter content were analyzed. It was observed that the SAGE showed a higher percentage of root colonization (about 10% more), and increases in the number of spores (about 26%) and dry matter (more than 13%) for the two VAM fungal spores than the soil inoculum. The results of this study suggested that the SAGE inoculum may be useful for the mass production of VAM fungi and also for the large scale production of VAM fungal fertilizer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.124-131
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• 1995

In order to find out optimum seed production date according to different defoliation and flooding period of Italian ryegrass, this studies with Tetrone were canied out on the experimental field of Honam Crop Experiment Station from 1986 to 1988. Treatments included cutting date of Nov. 20 and Apr. 10 and flooding period of 5-25 days. In soil after experiment, organic matter, phosphate and silicate content increased, but potassium content decreased 0.16% than that before experiment. Heading and maturing date of Nov. 20 cutting were same with those of non cutting, those of Apr. 10 cutting lates 6 days to heading date and 2 days to maturing date. Plant height and culm length of Nov. 20 cutting were sirniller to those of non cutting, those of Apr. 10 cutting were shorter and panicle length have no difference between non cutting and cutting. Lodging of cutting treatment was reduced than that of non cutting. Lodgin was increased as flooding period was long, also loding of all treatment occured at 30 days after heading. Two cutting times of Nov. 20 and Apr. 10 have the most fresh yield, while non cutting have the most dry matter yield. Optimun seed productin date was considered to suitable when 35 days after heading (Jun. 14), at this time, seed production was 1,640 to 2,640 kg/ha. Also if flooding j u r y have, seed production was good between 10 days and 15 days after flooding.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.1
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• pp.35-42
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• 1997

Many farmers apply cattle slurry on grassland, often in excess of crop N requirements, resulting in groundwater contamination. Our research objective was the development of grassland management systems that reduce undesirable sideeffects on other compartment of the ecosystem. Field experiment was conducted during 1991~1993 on sandy loam soil at AllgZiu south western Germany. Different sets of conditions were tested such as : zero fertilization, reduced sluny application$(l20kg N ha^{-1}yr^{-1})$, intensive sluny application(conventional sluny application, $240kg N ha^{-1}yr^{-1}$). The plots of the reduced slurry application had no significant effect on dry matter yields, digestible dry matter and net energy of herbage. Dry matter yield of zero fertilization was significantly lower than that of usual slurry application, however the content of Ca and Mg in herbage tended to increase. We conclude that reduced sluny application can be applied on grassland without adverse effects on dry matter production and quality of herbage.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.40-42
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• 2003

In order to examine the possibility that oil seed rape could be used as a forage fodder crop and to select the most suitable variety of forage rape at the southern area of Korea, two varieties of oil seed rape currently grown for oil production and six introduced varieties of forage rape with relatively high yield and high nutritional value were grown at the same condition and yield components were observed. Forage rape was superior to oil seed rape in terms of yield components, plant fresh weight and plant dry mater weight. Velox was superior to any other variety of forage rape in these characters. When plant dry matter weight of the rape was subdivided into four components such as a main stem, branch stems, main stem leaves and branch leaves, contribution of these components to plant dry matter weight was in the order of branch stems, branch leaves, the main stem and main stem leaves. Dry matter percentage of the rape ranged from 9.32 to 11.08 percent, which was somewhat low value. There was no significant difference between two groups of the rape in terms of dry matter percentage. Velox showed somewhat higher value in dry matter percentage.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.2
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• pp.197-203
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• 1993

The protection of dry matter and nitrogen from rumen degradation of dhaincha seed (Sesbania aculeate) meal was studied using nylon bag technique. The dhaincha seed meal was subjected to various heat treatments that included oven drying, autoclaving and boiling. Similar experiment was conducted with fish meal as reference for comparison. The oven-dried dhaincha meal was found to retain more dry matter and nitrogen than was found boiled or autoclaved meal. While autoclaving appeared to improve nitrogen and dry matter retention to some extent, boiling seemed to cause more loss of dry matter from nylon bag. Heat treatment caused high retention of nitrogen by fish meal. The calculated effective protein degradation was 80.4% and 83.2% for the oven dried fish meal and dhaincha seed meal whereas same values were 74.2% and 86.7% for autoclaved fish and dhaincha seed meal respectively at the outflow rate of 4.4% per hour. The in vitro study revealed higher digestibility for heat treated samples by pepsin. The dry matter, nitrogen and ash content of dhaincha seed meal were 85.93%, 5.93% and 7.31% respectively.