• Food Science and Biotechnology
• /
• 제17권5호
• /
• pp.983-989
• /
• 2008

Two flavonoids, isorhamnetin 3-O-$\beta$-D-glucopyranoside (1) and quercetin 3-O-$\beta$-D-glucopyranoside (2), from slander glasswort (Salicornia herbacea, Korean name hamcho) were isolated. Antioxidative and matrix metalloproteinase-9(MMP-9) inhibitory effects of these compounds were investigated in HT 1080 cell lines. These compounds suppressed the electron spin resonance (ESR) signal intensity on generation of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical in a free-cellular system. Their scavenging effects on generation of intercellular reactive oxygen species (ROS) also exhibited similar trends with DPPH radical in the free cellular system. Also, a control group combined only with Fe(II)-$H_{2}O_2$ resulted in DNA apoptosis by oxidative stress, whereas treatments with these compounds suppressed radical-mediated DNA damage. Intracellular glutathione (GSH) levels were slightly increased in the presence of compound 1 and 2. Moreover, these compounds led to the reduction of the expression levels of MMP-9 without cytotoxic influence. These results suggest that these compounds have a potential as a valuable natural antioxidant and MMP inhibitor related to oxidative stress. Therefore, these compounds not only can be developed as a candidate for a therapeutic potential but also a source for use as ingredients of health foods or functional foods to prevent metastasis involving MMP-9, closely related to ROS.

• 대한본초학회지
• /
• 제38권4호
• /
• pp.45-52
• /
• 2023

Objectives : Fine dust caused by environmental pollution cause oxidative damage and skin aging. In this study, The possibility of using the Codium fragile extract (CFE) as an anti-aging product for skin improvement was evaluated by confirming the protective effect of skin cells from PM10 (particulate matter 10) through inhibition of ROS and MMPs. Methods : In this study, elastase and collagenase inhibitory activities were evaluated. Cell viability was evaluated by treating keratinocytes (HaCaT cell line) with CFE at various concentrations. The cytoprotective effect from PM10 in keratinocyteswas evaluated using the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. ROS (reactive oxygen species) was measured in keratinocytes damaged by PM10 using DCF-DA (2′,7′-dichlorofluorescin diacetate) fluorescence staining. As an anti-aging effect of CFE, MMP-1 (matrix metalloproteinase-1) and MMP-1 (matrix metalloproteinase-9) inhibitory activities were evaluated. Results : As a result, CFE decreased the activity of elastase and collagenase. As a result of evaluating the toxicity of CFE, it is non-toxic at a concentration of 10 to 80 ㎍/㎖. Although cell viability of HaCaT cells treated with PM10 decreased, cell viability increased by 38% when treated with CFE 80 ㎍/㎖. Also, ROS decreased by 8.4%, and MMP-1 and MMP-9 decreased at CFE 80 ㎍/㎖. Conclusions : CFE showed excellent cell protection effect, and it is considered that it can be used in anti-aging products for skin improvement by effectively inhibiting ROS and MMPs from keratinocyte damage caused by fine dust.

• Journal of Microbiology and Biotechnology
• /
• 제27권2호
• /
• pp.242-250
• /
• 2017

Anti-photoaging effects of standardized Siegesbeckia glabrescens extract (SGE) and its major active compound kirenol were investigated using Hs68 human dermal fibroblasts and hairless mice, respectively. UVB-irradiated hairless mice that received oral SGE (600 mg/kg/day) showed reduced wrinkle formation and skinfold thickness compared with the UVB-irradiated control. Furthermore, SGE treatment increased the mRNA levels of collagen synthesis genes (COL1A1, COL3A1, COL4A1, and COL7A1) and activated antioxidant enzyme (catalase), while suppressing matrix metalloproteinase (MMP-2, -3, -9, and -13) expression. In Hs68 fibroblasts, kirenol also significantly suppressed MMP expression while increasing the expression of COL1A1, COL3A1, and COL7A1. Collectively, our data demonstrate that both SGE and kirenol attenuated UVB-induced photoaging in hairless mice and fibroblasts through inhibition of the mitogen-activated protein kinases and nuclear factor kappa B pathways, suggesting that SGE has potential to serve as a natural anti-photoaging nutraceutical.

• Journal of Microbiology and Biotechnology
• /
• 제29권1호
• /
• pp.11-20
• /
• 2019

Ecklonia cava, an edible marine brown alga (Laminariaceae), is a rich source of bioactive compounds such as fucoidan and phlorotannins. Ecklonia cava extract (ECE) was prepared using 70% ethanol extraction and ECE contained 67% and 10.6% of total phlorotannins and dieckol, respectively. ECE treatment significantly inhibited receptor activator of nuclear $factor-{\kappa}B$ ligand (RANKL)-induced osteoclast differentiation of RAW 264.7 cells and pit formation in bone resorption assay (p <0.05). Moreover, it suppressed RANKL-induced $NF-{\kappa}B$ and mitogen-activated protein kinase signaling in a dose dependent manner. Downregulated osteoclast-specific gene (tartrate-resistant acid phosphatase, cathepsin K, and matrix metalloproteinase-9) expression and osteoclast proliferative transcriptional factors (nuclear factor of activated T cells-1 and c-fos) confirmed ECE-mediated suppression of osteoclastogenesis. ECE treatment ($100{\mu}g/ml$) increased heme oxygenase-1 expression by 2.5-fold and decreased intercellular reactive oxygen species production during osteoclastogenesis. The effective inhibition of RANKL-stimulated osteoclast differentiation and oxidative stress by ECE suggest that ECE has therapeutic potential in alleviating osteoclast-associated disorders.

• 대한암한의학회지
• /
• 제15권1호
• /
• pp.47-61
• /
• 2010

Objective : The aim of this present study is to evaluate the inhibitory effect of allergen removed Rhus verniciflua (ARV) on Matrix Metalloproteinase-9 (MMP-9), Matrix Metalloproteinase-2 (MMP-2) which is considered to have a clinically important role in tumor metastasis. Methods : The inhibitory effects of standardized extract of ARV on the MMP-2, MMP-9 were investigated by spectrofluorometer while the inhibitory effects on the active MMP-2, pro MMP-2, pro MMP-9 were investigated by zymography. Antimetastatic effect of standardized extract of ARV was investigated in vitro on human fibrosarcoma cell (HT1080)'s invasion through Matrigel. Results : The standardized extract of ARV showed inhibitory effects on the active MMP-2 (IC50, $1.01{\mu}g$/ml), active MMP-9 (IC50, $2.5{\mu}g$/ml) depending on concentrations which was determined by spectrofluorometer. The standardized extract of ARV showed inhibitory effects on the active MMP-2, pro MMP-2, pro MMP-9 depending on concentrations which was determined by zymography. However its inhibitory effect on pro MMP-9 was relatively weaker rather than active MMP-2, pro MMP-2. The standardized extract of ARV showed inhibitory effects in vitro on human fibrosarcoma cell (HT1080)'s invasion through Matrigel according to concentration. Conclusions : These results indicate that standardized extract of ARV has antimetastatic effect through inhibit again MMP-2, MMP-9. Also its inhibitory effect is more powerful on active MMP-2, pro MMP-2 than on active MMP-9, pro MMP-9. It is necessary to conduct further studies on other MMP families, TIMP, and each component of standardized extract of ARV.

• Tuberculosis and Respiratory Diseases
• /
• 제71권3호
• /
• pp.172-179
• /
• 2011

Background: Statins can regulate the production of pro-inflammatory cytokines and inhibit MMP production or activation in a variety of types of cells. This study evaluated whether statins would inhibit MMP release from human lung fibroblasts, which play a major role in remodeling processes. Methods: This study, using an in-vitro model (three-dimensional collagen gel contraction system), evaluated the effect of cytokines (tumor necrosis factor-${\alpha}$, TNF-a and interleukin-$1{\beta}$, IL-1b) on the MMP release and MMP activation from human lung fibroblasts. Collagen degradation induced by cytokines and neutrophil elastase (NE) was evaluated by quantifying hydroxyproline. Results: In three-dimensional collagen gel cultures (3D cultures) where cytokines (TNF-a and IL-1b) can induce the production of MMPs by fibroblasts, it was found that simvastatin inhibited MMP release. In 3D cultures, cytokines together with NE induced collagen degradation and can lead to activation of the MMP, which was inhibited by simvastatin. Conclusion: Simvastatin may play a role in regulating human lung fibroblast functions in repair and remodeling processes by inhibiting MMP release and the conversion from the latent to the active form of MMP.

• 대한화장품학회지
• /
• 제32권3호
• /
• pp.129-134
• /
• 2006

본 연구는 곰취 추출물의 항산화, 지질과산화 억제 및 자외선 조사에 의해 유도된 MMP-1 발현에 대한 영향을 사람 섬유아세포를 이용하여 확인하였다 곰취의 DPPH와 superoxide radical 소거효과는 처리농도가 증가함에 따라 농도 의존적으로 소거효과를 나타냈으며, 각각 5mg/mL에서 82.3%와 79.3%로 DPPH와 superoxide radical을 소거하여 우수한 항산화 효과를 나타내었다. 곰취 추출물의 지질과산화 억제효과는 $500{\mu}g/mL$에서 97.0%로 지질과산화 억제효과도 우수하게 나타났다. 또한 자외선에 의해 증가된 사람 섬유아세포의 MMP-1 단백질 발현양은 곰취 추출물을 $100{\mu}g/mL$ 농도를 처리함으로써 약 35%로 감소되었다. 곰취 추출물은 항산화 효과, 지질과산화 억제효과 및 자외선 조사에 의해 유도되는 MMP-1 단백질 발현량을 조절하는 기능을 갖는 것을 확인하였다. 따라서 곰취는 항산화, 지질과산화 억제 및 자외선에 의해 유도되는 MMP-1 발현을 저해함으로써 항노화 소재로써 이용될 수 있을 것으로 사료된다.

• 한국미생물·생명공학회지
• /
• 제42권2호
• /
• pp.194-201
• /
• 2014

본 연구에서는 국내 자생버섯의 일종인 Clitocybe aurantiaca KCTC11143BP 균주가 생산하는 주름개선 화장품 신소재 clitocybin A의 발효생산 최적조건, 추출정제조건, 세포독성 및 자외선 조사에 따른 MMP-1 발현 저해활성을 규명하였다. C. aurantiaca 균주를 5 L 발효조를 이용하여 회분식으로 배양하였을 경우, PD 액체배지보다 YM 액체배지에서 양호하게 생육하는 것을 확인하였다. 300 L 용량의 대형 발효조에서 modified된 YM 배지를 이용한 유가식 배양으로 14일간 배양한 결과, 12.5 kg/120 L의 총 균체량을 얻었다. 발효 추출물로부터 항노화 소재인 clitocybin A를 추출정제를 한 후 HPLC를 실시하여 배양 4일째부터 clitocybin A가 생산되고 있음을 확인하였다. Clitocybin A 화합물의 세포독성을 확인하기 위하여 MTT assay를 실시한 결과, 100 ${\mu}g/ml$ 농도로 처리하였을 경우 $134.6{\pm}10.4%$로 오히려 세포가 증식하여 안전한 소재임을 확인하였다. 반면, 대조군으로 사용한 oleanolic acid는 25 ${\mu}g/ml$ 농도에서도 강한 세포독성을 나타냈다. 또한, clitocybin A는 100 ${\mu}g/ml$ 처리시 33.1%의 MMP-1 발현 저해활성 보여 주름개선 기능성화장품 소재로 매우 우수한 화합물임을 확인하였다.

• Journal of Ginseng Research
• /
• 제45권1호
• /
• pp.119-125
• /
• 2021

Background: Korean Red Ginseng (KRG) is a natural product with antiinflammatory and anticarcinogenic effects. We have previously reported that the endocrine-disrupting compound bisphenol A (BPA)-induced cyclooxygenase-2 (COX-2) via nuclear translocation of nuclear factor-kappa B (NF-κB) and activation of mitogen-activated protein kinase and promoted the migration of A549. Here, in this study, we assessed the protective effect of KRG on the BPA-induced reactive oxygen species (ROS) and expression of COX-2 and matrix metalloproteinase-9 (MMP-9) in A549 cells. Methods: The effects of KRG on the upregulation of ROS production and COX-2 and MMP-9 expression by BPA were evaluated by fluorescence-activated cell sorting (FACs) analysis, quantitative reverse transcription polymerase chain reaction, and western blotting. Antimigration ability by KRG was evaluated by migration assay in A549 cells. Results: KRG significantly suppressed the BPA-induced COX-2, the activity of NF-κB, the production of ROS, and the migration of A549 cells. These effects led to the downregulation of the expression of MMP-9. Conclusions: Overall, our results suggest that KRG exerts an antiinflammatory effect on BPA-treated A549 cells via the suppression of ROS and downregulation of NF-κB activation and COX-2 expression which leads to a decrease in cellular migration and MMP-9 expression. These results provide a new possible therapeutic application of KRG to protect BPA-induced possible inflammatory disorders.

• 생명과학회지
• /
• 제32권12호
• /
• pp.938-946
• /
• 2022

본 연구는 대풍자 에탄올 추출물에 대한 항산화 활성 및 주름생성 억제 활성을 확인하고자 하였다. 대풍자 에탄올 추출물의 항산화 활성을 확인하기 위하여 DPPH와 ABTS⁺ radical 소거능을 측정하였고 그 결과 추출물 1,000 ㎍/ml 농도에서 각각 73.5%와 74.4%의 높은 소거능을 나타냈다. 주름생성 억제 활성을 확인하기 위하여 collagenase 저해 활성을 실험하였으며, 그 결과 추출물 1,000 ㎍/ml 농도에서 78.8%의 유의할만한 우수한 결과를 보였다. 또한 추출물에 대한 세포의 독성을 확인하기 위해 MTT assay를 수행하였으며, 50 ㎍/ml 이하의 농도에서 약 91.7%의 생존율을 보여, 이후의 세포 실험은 50 ㎍/ml 이하의 농도에서 진행하였다. 섬유아세포인 CCD-986sk에 UVB (20 mJ/cm²)의 자극을 주고 대풍자 에탄올 추출물을 농도별로 처치한 후 procollagen type I과 MMP-1의 발현에 대한 영향을 확인하고자 ELISA 및 RT-PCR 분석을 하였다. 그 결과 대풍자 에탄올 추출물은 PIP의 생합성과 mRNA 발현은 농도의존적으로 증가하였으며, 특히 UVB만 자극한 Cont (각각의 생합성율은 50.3%와 45.8%)과 비교하였을 때 50 ㎍/ml의 농도에서 각각 약 64.2%와 83.4%의 생합성을 보였다. MMP-1의 protein과 mRNA 발현에 대한 결과는 농도의존적으로 감소했음을 확인하였으며, 50 ㎍/ml의 농도에서 각각 약 48.7%와 35.9%의 낮은 발현율을 보였다. 이와 같은 결과를 바탕으로 본 연구는 대풍자 에탄올 추출물이 주름억제 기능성 소재로써의 활용 가능성을 제시해 줄 것으로 사료된다.