• The Korean Journal of Mycology
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• v.26 no.1 s.84
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• pp.16-19
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• 1998

A study was carried out to explore the method for the germination of the spore of Ganoderma. Three strains of Ganoderma were examined using 2 different media such as PDA and LBA at three different temperatures. As it has been known the germination rate was very low under all the conditions examined. G. lucidum ASI 7004 showed 0.02% germination on both media at $25^{\circ}C$ and $30^{\circ}C$ respectively. The germination rate of ASI 7091 was 0.05% on PDA medium at $30^{\circ}C$. The germination rate of G. oregonense ASI 7067 was 0.67% on PDA at $35^{\circ}C$. LBA medium was found to be inadequate for the germination of Ganderma species in this study. In a consequent study, four mating types of G. oregonense such as $A_1B_1,\;A_1B_2,\;A_2B_1\;and\;A_2B_2$ were identified.

• Journal of Embryo Transfer
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• v.21 no.2
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• pp.109-119
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• 2006

This study was conducted to examine relationship between vaginal cytology and reproductive hormones during the estrous cycle and to provide basic data to estimate for ovulation time and optimal mating time in 6 beagle dogs The duration of proestrus, estrus and diestrus were $8.5{\pm}1.4,\;10.0{\pm}1.4\;and\;54.0{\pm}2.8$ days at pregnant respectively, and $7.9{\pm}2.1,\;9.5{\pm}0.7\;and\;62.0{\pm}11.3$ days at non-pregnant respectively. The duration of interestrous intervals were $246.2{\pm}24.5$ days at pregnancy, and $175.3{\pm}34.5$ days at non-pregnancy. The duration of interestrous intervals at pregnancy was longer than that of non-pregnancy. A characteristic features of vaginal cytology during the estrous cycle were the high proportion of superficial cell, anuclear cell and erythrocyte in proestrus and estrus, parabasal cell, small intermediate cell and leukocyte in diestrus, and parabasal cell and small intermediate cell in anestrus, respectively. Cornification index (CI) in proestrus and estrus were significantly higher than that of CI in diestrus and anestrus. Plasma progesterone concentration was below 1.0 ng/ml at the first day of vulval bleeding at pregnancy and non-pregnancy, and then it was above 2.0 ng/ml at Day -2 in all bitches. When plasma progesterone concentration was first increased above 4.0 ng/ml, it was the second day after the first day of male acceptance. Plasma progesterone concentration showed above 40 ng/ml on Day $20{\sim}22$ in all bitches, and then it was gradually decreased until Day 35. Plasma progesterone concentration at pregnancy was higher than that of non-pregnancy from Day 35 to Day 63. Plasma estradiol-$17\;{\beta}$ concentration was above 9.0 pg/ml at the first day of vulval bleeding, and it showed 26.4 pg/ml on Day -2. When it was timed from the first day of male acceptance (Day 0), plasma estradiol-$17{\beta}$ concentration showed a peak on Day 0 and plasma progesterone concentration was first increased above 4.0 ng/ml on Day 2 which was the third day after plasma estradiol-$17{\beta}$ peak. CI was first increased above 80 and 90% on Day -1 and Day 1, respectively. CI was maintained above 80% from Day -1 to Day 8 (10 days) and above 90% from Day 1 to Day 6 (6 days), respectively. CI was maintained above 80% from Day 0 to Day 8 (9 days) and above 90% from Day 1 to Day 6 (6 days), respectively. Plasma progesterone concentration was first increased above 4.0 ng/ml on the second day after the day which CI was first increased above 90%. In conclusion, beagle bitches ovulated on the second day after the day which CI was first increased above 90% and on the day which plasma progesterone concentration was first increased 4.0 ng/ml, and it was estimated that the optimal mating time was the day which the second day after CI was first increased above 90% and plasma concentration was between $2{\sim}25ng/ml$. The measurement of plasma progesterone was used to determine of and accurate ovulation time and the optimal mating time, but vaginal cytology is low-priced and simple method to estimate estrous cycle, optimal mating time and ovulation time.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.26 no.3
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• pp.137-143
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• 2014

A gasket is known as an important component for mechanical sealing between mating surfaces. In case of the refrigerator, a gasket is generally installed into the edge line of a door to prevent cold air leaking out from the inside of a cabinet. The gasket shape used in a refrigerator is very complicated; it has a few deformable small rooms and wings (upper part) and inserting part (lower part) into the door. By the intricate shape, it takes much time and trouble on analysis by using a full-shape model. To settle down this problem, an effective design way of a gasket is proposed in this work. The inserting part is parametrically designed using FEM without considering the complicate upper part, Through this work, computational time and efforts are much reduced comparing to the full-shape analysis method. And the performance of a newly designed gasket was evaluated qualitatively.

• Journal of Ocean Engineering and Technology
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• v.29 no.2
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• pp.135-142
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• 2015

The float over method is the most preferred method for installing heavy topside onto a jacket platform. A very complex platform with multiple jacket structures on a specific field requires multiple installation procedures. This study validated the installation of two topsides using a single installation barge to reduce the operation and installation cost. The hydrodynamic properties of the installation barge during the installation of two topsides were calculated. The tension and fender forces during docking were investigated to show the validity of the proposed dual topside installation method. In conclusion, the operational safety of the proposed procedure was validated through the calculation of the motion of the installation vessel and loads on the jacket legs.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.25 no.6
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• pp.916-922
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• 2001

A mechanical face seal is a tribo-element intended to control leakage of working fluid at the interface between a rotating shaft and its housing. Leakage of working fluid decreases drastically as the clearance of the mating seal faces gets smaller. But the very small seal clearance results in an increased reduction of seal life because of high wear and heat generation. Therefore, in the design of mechanical face seals a compromise between low leakage and acceptable seal life is important, and it presents a difficult and practical design problem. A fluid film or sealing dam geometry of the seal clearance affects seal lubrication performance very much, and thereby it is one of the main design considerations. In this study the Reynolds equation for the sealing dam of mechanical face seals is numerically analyzed, using the Galerkin finite element method, which is readily applied to various seal geometries. Film pressures of the sealing dam are analyzed, including the effects of the seal face coning and tilt. Then, lubrication performances of the seals, such as opening forces, restoring moments, leakage, and dynamic coefficients, are calculated, and they are compared to the results obtained by the narrow seal approximation.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.20 no.2
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• pp.209-229
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• 2022

This work provided a review of three techniques-(1) spectrochemical, (2) electrochemical, and (3) spectroelectrochemical-for molten salt medias. A spectroelectrochemical system was designed by utilizing this information. Here, we designed a spectroelectrochemical cell (SEC) and calibrated temperature controllers, and performed initial tests to explore the system's capability limit. There were several issues and a redesign of the cell was accomplished. The modification of the design allowed us to assemble, align the system with the light sources, and successfully transferred the setup inside a controlled environment. A preliminary run was executed to obtain transmission and absorption background of NaCl-CaCl₂ salt at 600℃. It shows that the quartz cuvette has high transmittance effects across all wavelengths and there were lower transmittance effects at the lower wavelength in the molten salt media. Despite a successful initial run, the quartz vessel was mated to the inner cavity of the SEC body. Moreover, there was shearing in the patch cord which resulted in damage to the fiber optic cable, deterioration of the SEC, corrosion in the connection of the cell body, and fiber optic damage. The next generation of the SEC should attach a high temperature fiber optic patch cords without introducing internal mechanical stress to the patch cord body. In addition, MACOR should be used as the cell body materials to prevent corrosion of the surface and avoid the mating issue and a use of an adapter from a manufacturer that combines the free beam to a fiber optic cable should be incorporated in the future design.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.9
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• pp.2976-2990
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• 2022

The adaptive adjustment of reference or weight vectors in decomposition-based methods has been a hot research topic in the evolutionary community over the past few years. Although various methods have been proposed regarding this issue, most of them aim to diversify solutions in the objective space to cover the true Pareto fronts as much as possible. Different from them, this paper proposes a knee point-based reference vector adaptive adjustment strategy to concurrently balance the convergence and diversity. To be specific, the knee point-based reference vector adaptive adjustment strategy firstly utilizes knee points to construct the adaptive reference vectors. After that, a new fitness function is defined mathematically. Then, this paper further designs a many-objective evolutionary algorithm with knee point-based reference vector adaptive adjustment strategy, where the mating operation and environmental selection are designed accordingly. The proposed method is extensively tested on the WFG test suite with 8, 10 and 12 objectives and MPDMP with state-of-the-art optimizers. Extensive experimental results demonstrate the superiority of the proposed method over state-of-the-art optimizers and the practicability of the proposed method in tackling practical many-objective optimization problems.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2000.06a
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• pp.201-210
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• 2000

The noncontacting groove end face seal is one of various approaches to sealing gases with a single seal. Gas pumped into groove maintains operating gap and lubricates between primary ring and mating ring. So it removes heat and decreases face wear. In this paper, K-type and T-type grooved seals have been analyzed numerically using the finite element method. It explains the effects of groove shapes in gas seals along rotating speeds with a temperature gradient, face distortion, stress and so on. The calculated FEM results show the operating gap and rotating speed are strongly related to the leakages of a gas seal and that T-type groove seal shows a good thermal performance compared to K-type groove seal.

• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.335-340
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• 1993

The development of efficient method for the production of transgenic mice has been investigated in our laboratory. This study was conducted to develop the artificial insemination in the mouse. Spermatozoa were collected from the cauda epididymis of ICR males(age:12~15 weeks, Body weight : 30g) and artificially inseminated into the intrauterine via cervix of hormone-primed ICR females(age: 6~8 weeks, body weight: 25g) using the capillary tube, 200~300 $\mu$m in inner diameter. The effect of concentration of sperm(80$\times$104, 40$\times$104, 20$\times$104, 10$\times$104, 5$\times$104, 3$\times$104, 1$\times$104/20${mu}ell$. The artificial insemination was succeeded but fertilization rate was very low(5~15%) compared to the natural mating and 59 normal youngs born from 60 females. Therefore, our findings suggest that it is possible to produce the great number of mice from the same orgin of male by artificial inseminatin. However, the lower pregnancy rate has to be solved to used broadly the artificial insemination in mouse.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.1350-1353
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• 2007

An experimental method based on contact resonance is developed to extract the contact parameters of mechanical joints under various clamped conditions. Mechanical joint parameters of shear contact stiffness and damping were extracted for different physical joint parameters such as surface finish of the mating surfaces, the presence of lubrication, the effect of the clamping pressure, and shear load. It was found that the shear contact stiffness values decreased with increasing clamping load and increased with increasing shear loading. Contact damping ratio values were almost constant with clamping load, but decreased with increasing shear load. Moreover, rough surfaces exhibited the highest shear stiffness and contact damping compared to smooth surfaces.