• Journal of fish pathology
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• v.32 no.2
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• pp.97-104
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• 2019

The purpose of this study was to investigate the antimicrobial resistance and resistance transfer of Vibrio parahaemolyticus and Morganella morganii isolated from fish products purchased from fish markets in Yeosu April - December 2017. These bacteria were identified by biochemical test and PCR results, and the transfer of antimicrobial resistance was confirmed by the broth mating method. To isolate the transconjugants formed during conjugation, TSA medium containing 50 ㎍/ml of ampicillin (AMP), and 150 ㎍/ml of streptomycin (SM) or 30 ㎍/ml of oxytetracycline (OT) was used. M. morganii isolates showed low susceptibility to AMP, amoxicillin (AML), and colistin (CT), erythromycin, OT, and tetracycline, compared to V. parahaemolyticus resistance to AMP, AML, and CT. The conjugation of V. parahaemolyticus or M. morganii with Escherichia coli resulted in the separation of V. parahaemolyticus and M. morganii showing SM resistance as transconjugants. Meanwhile, Edwardsiella tarda transconjugants showing AMP and AML resistance were obtained from the broth mating of V. parahaemolyticus and E. tarda. But the transfer of the VPA0477 which is a β-lactamase gene of V. parahaemolyticus was not confirmed. These results suggest that resistance transfer between pathogenic bacteria is bidirectional and progresses in a wide variety of patterns.

• The Korean Journal of Mycology
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• v.25 no.1 s.80
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• pp.26-29
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• 1997

Pyrimidine auxotrophic basidiospores of Pleurotus sajor-caju were selected using positive selection method. Wild type basidiospores could not grow on minimal medium containing the pyrimidine analog 5'-fluoro-orotic acid (5'-FOA) whereas pyrimidine auxotrophs grew normally. After treatment of basidiospores with ultraviolet light, a total of 13 pyrimidine auxotrophic basidiospores were isolated among 24 5'-FOA resistant mutants. These mutants require the pyrimidine such as uracil, cytosine, thymine. Mating type group and growth rate of their mutants were determined.

• Journal of Advanced Marine Engineering and Technology
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• v.10 no.2
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• pp.136-145
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• 1986

The perfect and accurate methods to control the wear are not made clear so far. For this phenomenon only mating surface has been studied. In order to control the wear the essence of it has to be made clear. It is reported that adhesive wear might occure as a result of plastic deformation, the fracture and removal or transfer asperities on close contacting surfaces. On this view point the plastic flow was attempted to compare with fluid or electromagnetic flow. The partial differential equations of equilibrium for the plane strain deformation will make use of the method of characteristics. The characteristic curves or characteristics of the hyperbolic equation coincide with the slip lines by R. Hill's papers. By Hencky's stress equation, it is evident that if P and .phi. are prescribed for a boundary condition then it may be possible to proceed along constant .alpha. and .betha. lines to determine the value of the hydrostatic pressure everywhere in the slip line field net work. A wedge formation mechanism has been considered for an explanation of this matters. The analysis shows that there is a critical value, which depends on the hardness ratio and the shear stress on the interface, for the top angle of asperity is less than this critical value, the asperity can yield plastically despite of being harder than the mating surface.

• Tribology and Lubricants
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• v.36 no.5
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• pp.290-296
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• 2020

When two layers of carbon nanotube (CNT) arrays are loaded to mate, the free ends of individual CNTs come into contact at the interface of the two layers. This leads to a higher contact resistance due to a smaller contact region. However, when the free CNT ends of one array penetrate into the mating array, the contact region increases, effectively lowering the contact resistance. To explore the penetration of mating CNTs, we perform molecular dynamic simulations of a simple unit cell model, incorporating four CNTs in the lower array layer coupled with a single moving CNT on the upper layer. The interaction with neighboring CNTs is modelled by long-range carbon bond order potential (LCBOP I). The model structure is optimized by energy minimization through the conjugate gradient method. A NVT ensemble is used for maintain a room temperature during simulation. The time integration is performed through the velocity-Verlet algorithm. A significant vibrational motion of CNTs is captured when penetration is not available, resulting in a specific vibration mode with a high frequency. Due to this vibrational behavior, the random behaviors of CNT motion for predicting the penetration are confirmed under the specific gap distances between CNTs. Thus, the probability of penetration is examined according to the gap distance between CNTs in the lower array and the aspect ratio of CNTs. The penetration is significantly affected by the vibration mode due to the van der Waals forces between CNTs.

• Korean Journal of Animal Reproduction
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• v.8 no.1
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• pp.10-15
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• 1984

This experiment was carried out to study the egg transports and distribution patterns in the oviducts of superovulated rabbits by administrations of PGF2$\alpha$. Group I (3 does) and Group II (3 does) were killed at 24 hours, 48 hours after mating, respectively. Group III (6 does0 received a single injection of 5mg/Kg of body weight of PGF2$\alpha$ at 24 hours after mating and killed at 4 hours after PGF2$\alpha$ administration. The oviducts were frozen in LN-gas, thawed using Howe's method, and cleared in Benzyl-Benzoate sol. using Orsini's technique. The location of the eggs and the AIJ. were identified using transmitted light from a dissecting microscope. The results obtained were as follows: 1. The average numbers of detected eggs from the oviduct were 28.6 (26-32), 26.0 (24-29), and 27.1 (22-34) in Group I, II, and III, respectively. 2. Detection rate was 88.7%, 76.4%, and 84.9% in Group I, II, and III, respectively. 3. The positionof all AIJ. was 44.2% to 55.0%(av. 49.5%) of the distance from the fimbriae to the UTJ.(Utero-Tubal Junction). 4. The changes of egg distribution patterns were as follows: Group I Ampulla : 52.3%, Isthmus : 47.7%, Group II Ampulla : 8.9%, Isthmus : 91.1% Group III Ampulla : 8.0% Isthmus : 92.0%.

• Journal of the Korean Society of Clothing and Textiles
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• v.11 no.2 s.24
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• pp.23-36
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• 1987

The purpose of the suudy was to develop a computer program for pattern mating of women's pant's. Computerization of the pattern making process was expected to provide higher accuracy and efficiency in pattern mating. The VAX-11/750 Computer and the CALCOMP PLOTTER 965 were used in this study. The procedures of the study were as follows : 1. A slacks pattern was selected. 2. The co-ordinate points were indicated relative location of all necessary in draffing. Total sixty co-ordinate points were instituted from (AX(1), AY(1)) to (AX(24), AY(24)), from (BX(1), BY(1)) to (BX(36), BY(36)). 3. A program for drafting was developed. Refer to (Table 3). 4. The procedures of drading of standard size were accomplished by using same method. The program was developed to drafting pattern for women by putting indivisual body measurement. The body measurements for stacks pattern were as follows : slacks length. Croach length. Hip length. nip circumference. waist circumference.

• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• Proceedings of the KIEE Conference
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• 2003.07c
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• pp.1451-1453
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• 2003

Shrink fitting is often used to replace conventional mechanical fasteners and fastening methods. Localized heating of the mating surface provides temporary expansion and allows slip fit assembly. The resulting interference fit exhibits exceptional strength without surface deformation at ambient temperatures. We studied an analysing method to find out a deformation of motor axis with shrink fitting of thermal expansion.

• Journal of Veterinary Clinics
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• v.4 no.2
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• pp.457-461
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• 1987

A comparative study on the two techniques of uterine flushing in superovulated sheep was conducted. The first method was carried out by flushing of the horn through a Foley catheter inserted into the uterine horn (method I ). The second method involved flushing of the horn with the medium injected into uterine tip nea. the utero-tubal junction through a Foley catheter inserted into uterine horn(method II). Superovulation was carried out on day 8 of the estrous cycle by a FSH-PG method. Ova were flushed on 6 or 7 days after mating. The recovery rates in methods I and II were 68.0%(51/75) and 43.6%(17/39), respectively(p<0.05). It indicated that method I is effective technique for flushing of the sheep ova from the uterus.

• Korean Journal of Veterinary Research
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• v.29 no.3
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• pp.303-313
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• 1989

To investigate the epidemiological trait of intestinal diseases of animals caused by thermophilic Campyllobacter spp., isolation of etiological agent was carried out and the profiles of plasmids and the transfer of resistance plasmid in the isolated Campylobacter spp. were examined. The results were as follows. 1. A total of 110 isolates of C jejuni and C coli were subjected to the test for the presence of plasmid DNA. Of the isolates examined, 60% of the isolates were noted to harbor plasmid DNA. Plasmid occurrencer ate from pigs, chickens and cattle were 76.2%, 61.7% and 37.7%, respectively. The plasmids of a large molecular weight, ranging from 36 Md to 86Md, were identified with the strains of tetracycline resistant. 2. Transfer frequency of tetracycline resistant plasmids was higher in the case of the filter mating method than in the broth mating method by the factor of 10~1,000. 3. Tetracycline resistant plasmids of C jejuni were transferrable to C jejuni and C coli by conjugation. In a low frequency, the transfer of tetracycline plasmid was also possible to Vibrio parahemolyticus. However, it was impossible to transfer to Streptococcus fecalis, E coli and Vibrio cholerae. 4. Tetracycline resistant plasmids of C jejuni were impossible to transfer to Campylobacter spp. and related bacteria by transformation.