The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

A Positive Selection for Pyrimidine Auxotrophic Mutants from Basidiospores of Pleurotus sajor-caju Using 5'-Fluoro-orotic acid

5'-Fluoro-orotic acid를 이용한 여름느타리버섯의 pyrimidine 영양요구성 균주의 positive 선발

  • Kim, Beom-Gi (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Park, Soo-Chul (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Jeong, Mi-Jeong (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Yoo, Young-Bok (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Ryu, Jin-Chang (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Kwon, Suk-Tae (Department of Genetic Engineering, Sung Kyun Kwan University)
  • 김범기 (농업과학기술원 분자유전과) ;
  • 박수철 (농업과학기술원 분자유전과) ;
  • 정미정 (농업과학기술원 분자유전과) ;
  • 유영복 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과) ;
  • 권석태 (성균관대학교 유전공학과)
  • Published : 1997.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Pyrimidine auxotrophic basidiospores of Pleurotus sajor-caju were selected using positive selection method. Wild type basidiospores could not grow on minimal medium containing the pyrimidine analog 5'-fluoro-orotic acid (5'-FOA) whereas pyrimidine auxotrophs grew normally. After treatment of basidiospores with ultraviolet light, a total of 13 pyrimidine auxotrophic basidiospores were isolated among 24 5'-FOA resistant mutants. These mutants require the pyrimidine such as uracil, cytosine, thymine. Mating type group and growth rate of their mutants were determined.

여름느타리버섯의 담자포자에 자외선을 조사하여 5'-fluoro-orotic acid(FOA) 선발배지에서 pyrimidine 영양요구성 균주를 직접 선발하였다. 24개의 5'-FOA 저항성 균주중 13개의 pyrimidine 영양요구성 균주를 선발할 수 있었으며, 이들의 mating type group과 성장속도를 측정하여 형질전환을 위한 최적의 DNA수용체를 선발할 수 있었다.

Keywords

References

  1. Biochem. Biophysic. Res. Commun. v.112 Transformation of Aspergillus nidulans by the orotidine 5'-monophosphate decarboxylase gene of Neurospora crassa Ballance, D.J.;Buxton, F.P.;Tuner, G
  2. Mol. Gen. Genet. v.197 A positive selection for mutants lacking orotidine-5'-phosphate decaboxyase activity in yeast: 5-fluoro-orotic acid resistance Boeke, J.D.;Croute, F.;Finik, G.R.
  3. Curr. Genet. v.24 Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus Campuzano, V.;Valle, del P.;Vicente, de J.I.;Eslava, A.P.;Alvarez, M.I.
  4. Korean J. Mycol. v.12 Selection of Pleurotus sajor-caju suitable species for cultivation under summer climatic conditions in Korea Go, S.J.;Byun, M.O.;You, C.H.;Park, Y.H.
  5. Curr. Genet. v.11 Transformation of Aspergillus niger using the homologous orotidine-5'-monophosphate-decarboxylase gene Goosen, T.;Bloemheuvel, G.;Gysler, C.;Debie, D.A.;Broek, H.J. Vander;Klaas swart.
  6. Korean J. Mycol. v.14 Isolation of auxotrophic mutants from basidiospores of Pleurotus cornucopiae Lee, Y.H.;Park, Y.H.;Yoo, Y.B.;Min, K.H.
  7. Mycoscience v.36 Homologous and heterologous gene transfer systems in basidiomycetes Noel, T.;Labarere, J.
  8. Curr. Genet v.22 Recovery of recombinant plasmids from Pleurotus ostreatus transformants Peng, M.;Singh, N.K.;Lemke, P.A.
  9. Mycologia v.64 Genetic analysis of the life cycle of Agaricus bisporus Raper, C.A,;Raper, J.R.;Miller, R.E.
  10. Mycologia v.69 Sexuality of an edible mushroom Pleurotus sajor-caju Roxon, J.E.;Jong, S.C.
  11. Korean J. Mycol. v.13 Isolation of auxotrophic mutants from basidiospores of Lentinus edodes Yoo, Y.B.;You, C.H.;Park, Y.H.