• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.501-507
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• 2019

Objective: The Agu is the only native pig breed in Japan, which is reared in Okinawa prefecture, the southernmost region in Japan. Its origins are considered to be of Asian lineage; however, the genetic background of the Agu is still unclear. The objective of this study was to elucidate the maternal lineage of the Okinawa indigenous Agu pig with the use of the mitochondrial DNA (mtDNA) control region. Methods: The mtDNA control regions of Agu pigs were sequenced and the phylogenetic relationship among Agu, East Asian and European pigs was investigated with the use of 78 Agu individuals. Results: Twenty-seven polymorphic sites and five different haplotypes (type 1 to type 5) were identified within the Agu population. Phylogenetic analysis indicated that types 1 and 2 were included in East Asian lineages; however, the remaining types 3, 4, and 5 were of European lineages, which showed a gene flow from European pigs in the 20th century. Sixty-seven out of 78 Agu individuals (85.9%) possessed mtDNA haplotypes 1 and 2 of the East Asian lineage, which were identical to two haplotypes of ancient mtDNA (7,200 to 1,700 years before the present) excavated at archaeological sites in Okinawa. Conclusion: This study confirmed that the East Asian lineage is dominant in the maternal genetic background of the Agu population, supporting the hypothesis that the ancestors of the Agu pig were introduced from the Asian continent.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1484-1490
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• 2004

Mitochondrial DNA haplotypes from the displacement-loop (D-loop) region (436 bp) were genotyped and sequenced in Japanese Black beef cattle raised in the same herd. Correlation coefficients between mitochondrial DNA haplotypes, maternal lineage, birth weight, preweaning average daily gain, weaning weight, post weaning average daily gain and yearling weight were computed. The objective was to study the relationship between maternal and postnatal growth traits and to investigate if postnatal growth of calves to yearling age could be accurately predicted from mitochondrial DNA haplotypes. Results of the phylogenetic analysis revealed 17 maternal lineages and four mitochondrial DNA haplotypes. There were strong, positive and highly significant (p<0.001) correlations among maternal traits ranging from 0.52 to 0.98. Similarly, among postnatal growth traits, most of the correlations were also strong, positive and highly significant (p<0.001); the highest correlation of 0.94 was between preweaning average daily gain and weaning weight. However, correlations between mitochondrial DNA haplotypes and postnatal growth traits were very low, mostly negative and non-significant (p>0.05) ranging from -0.05 to 0.1. Prediction of postnatal growth from mitochondrial DNA yielded very low $R^{2}$ values ranging from 0.002 to 0.019. It was concluded that mitochondrial DNA polymorphism has no significant association with postnatal growth from birth to yearling age, and by implication, nuclear rather than cytoplasmic DNA, accounts for most of the genetic variation observed in postnatal growth of Japanese Black cattle. Therefore, mitochondrial DNA genotyping at an early age has no bearing on the accurate prediction of the future growth performance of calves.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.3
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• pp.567-570
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• 1992

Variance components were estimated for calf daily gain from birth to 45 days of age in small (S), medium (M) and large (L) lines of beef cattle. Analyses involved records collected on 682 (S), 510 (M) and 228 (L) calves in Iowa, USA from 1978 to 1986. Cytoplasmic lines were determined based on the foundation female in the maternal lineage of each animal. Data were analyzed separately by size line using a derivative-free restricted maximum likelihood procedure under an animal model including additive direct (a), additive maternal (m), cytoplasmic lineage effects and covariance (a, m). The heritabilities for direct and maternal, and the cytoplasmic effects, were 0.13, 0.35 and 0.00 for S, 0.14, 0.32 and 0.00 for M, and 0.05, 0.33 and 0.03 for L. Genetic correlations (a, m) for S, M and L were -0.33, -0.57 and -1.00, respectively. The maternal genetic effect was the most important for calf growth between birth and 45 dyas of age and cytoplasmic variances were not important in any line.

• Journal of the Korean Home Economics Association
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• v.34 no.4
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• pp.357-371
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• 1996

This study explores the perspective of mothers who have children at teens concerning grandparents' role and grandchildren's role, especially focusing on the similarity and difference according to lineage. The analysis indicates as below. 1. Mothers highly expect the roles of grandparents which are related to the developing of self-identity of grandchildren at teens. There is much similarity between mothers; view on paternal grandparents' role and maternal grandparents' role. However the results support that paternal grandparents' role is more central than maternal grandparents in influencing to the development of self identity of grandchildren. 2. Grandchildren's role toward paternal grandparents is expected much more positively than grandchildrens' role toward maternal grandparents. 3. Mother's perspective on grandparents' role and grandchildren's role is associated with mother's view on the importance of grandparents in the lives of grandchildren.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.471-476
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• 2007

Little is known about the origin and genetic diversity of swamp buffaloes in China. To obtain more knowledge on genetics of the water buffalo in China, the complete mitochondrial D-loop sequences of 30 samples from 6 native types were investigated. The results revealed 12 mitochondrial haplotypes with 50 polymorphic sites. Among these polymorphic sites, there were 49 transitions and 1 transversion. The average nucleotide diversity and haplotype diversity estimated from mtDNA D-loop region in 6 Chinese water buffalo types were 0.00684 and 0.798, respectively, showing rather abundant mitochondrial genetic diversity. The Neighbor-Joining (NJ) tree of mtDNA of Chinese water buffaloes was constructed according to the 12 haplotypes. The NJ tree indicated two lineages being designated lineage A and lineage B, in which lineage A was predominant, and lineage B was at low frequency. The new lineage B was first discovered and defined in 6 Chinese water buffalo types. These results showed that two different maternal lineages were involved in the origin of domestic swamp buffaloes in China and the lineage B was probably an introgression from Southeast Asian buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.645-652
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• 2007

The origin of domestic donkeys in China has been controversial. To clarify the origin of Chinese domestic donkeys, we investigated the partial mitochondrial D-loop sequences of 126 samples from 12 native breeds. The results revealed two mitochondrial origins, lineage Somali and lineage Nubian of African wild ass detected in Chinese domestic donkeys. Lineage Somali was predominant in Chinese domestic donkey breeds. The pattern of genetic variation in ass mtDNA D-loop sequences indicated that the two lineages Somali and Nubian from China had undergone population expansion events. In a combined analysis of lineages Somali and Nubian between previously published sequences from other countries/regions and sequences of Chinese domestic donkeys, the results indicated that the two lineages of Chinese domestic donkeys were from Africa and supported the African maternal origins of Chinese domestic donkeys. There was no obvious geographical structure in Chinese domestic donkey breeds, but the population showed abundant mtDNA diversity. The spread routes of Chinese domestic donkeys were also discussed.

• 한국노년학
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• v.34 no.2
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• pp.277-297
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• 2014

This study investigates the predictors of the relationship quality between grandparents and grandchildren in emerging adulthood. Participants were 501 grandchildren with at least one living maternal/paternal grandparents. According to the t-test, the frequency of contact with maternal grandparents, the similarity of value to paternal grandparents, and the affective solidarity with paternal grandparents were significantly different depending on the grandchildren's sex. Results of multiple regression analyses indicated that the relationships of father-grandparents and mother-grandparents, the frequency of contact, and the similarities of value significantly predicted the affective solidarity between grandparents and grandchildren. However, the relative predictive power of these variables was different by the lineage and the grandchildren's sex.

• Korean Journal of Plant Taxonomy
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• v.49 no.4
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• pp.282-293
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• 2019

Broussonetia × kazinoki Siebold has long been utilized as a major component in the manufacturing of Korean traditional paper, hanji, and has been suggested as a hybrid species of B. papyrifera and B. monoica. By applying three molecular markers, chloroplast (cp) ndhF-rpl32 IGS, a nuclear ribosomal internal transcribed spacer, and the TOPO6 gene, the hybrid origin of B. × kazinoki is tested. As a result, B. × kazinoki in Korea is demonstrated to be a hybrid of B. monoica × B. papyrifera, most likely formed naturally in Korea. The cp haplotypes detected provided information about the origins and genetic diversity of the maternal lineage B. monoica and paternal lineage B. papyrifera. The two nuclear markers were supplemented to each other, leading to the discovery of introgression in Broussonetia.

• Survey Research
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• v.13 no.1
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• pp.89-112
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• 2012

This study aims to explore emotional closeness between grandparents and grandchildren in Korean families with a focus on the lineage. The effects of the geographical proximity and the normative aspect as well as intergenerational financial, instrumental, and emotional supports were taken into consideration to accounting for the grandparent-grandchildren affective solidarity. Research questions are addressed using the data of "Survey on Generational Solidarity and Differences in Cultural Experience and Perception in Korea", and a series of multinomial regression model were conducted. Findings indicate that the salient factor to boost grandchildren's affective solidarity with paternal grandparents is financial transfers between grandparents and parents. By contrast, all types of intergenerational supports affected grandchildren's emotional closeness toward maternal grandparents. Geographical proximity was associated with the affective solidarity between grandchildren and maternal grandparents. The effects of normative solidarity were not shown at both lineages.

• Journal of Life Science
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• v.21 no.3
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• pp.341-348
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• 2011

In an effort to gain greater understanding of the maternal lineages of the Jeju native pig (JNP), we analyzed the mitochondrial DNA (mtDNA) CYTB gene and compared it with those of other pig breeds. PCR-RFLP analysis was conducted with six pig breeds including JNP, and then the RFLP patterns allowed for the separation of the pig breeds into two distinct haplotypes (mtCYTB1 and mtCYTB2). The JNP CYTB sequences were detected in both the European and Asian breed clusters on the phylogenetic tree. The J2 group was sorted with the indigenous cluster of Asian pig lineages and was related closely to Chinese native pig breeds, but a second group, J1, was sorted with the European pig lineages and appeared to be related to Spanish Iberian native pigs, rather than to Asian breeds. These results indicate that the JNP currently raised on Jeju Island have two major maternal origins estimated in Asian and European pigs. We concluded that the JNP that share a common lineage with indigenous Asian pigs were domesticated in the distant past, originating from pigs that were already being raised elsewhere at that time, and that the European pig breeds introduced in the twentieth century have also contributed to the formation of this pig population.