• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.501-507
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• 2019

Objective: The Agu is the only native pig breed in Japan, which is reared in Okinawa prefecture, the southernmost region in Japan. Its origins are considered to be of Asian lineage; however, the genetic background of the Agu is still unclear. The objective of this study was to elucidate the maternal lineage of the Okinawa indigenous Agu pig with the use of the mitochondrial DNA (mtDNA) control region. Methods: The mtDNA control regions of Agu pigs were sequenced and the phylogenetic relationship among Agu, East Asian and European pigs was investigated with the use of 78 Agu individuals. Results: Twenty-seven polymorphic sites and five different haplotypes (type 1 to type 5) were identified within the Agu population. Phylogenetic analysis indicated that types 1 and 2 were included in East Asian lineages; however, the remaining types 3, 4, and 5 were of European lineages, which showed a gene flow from European pigs in the 20th century. Sixty-seven out of 78 Agu individuals (85.9%) possessed mtDNA haplotypes 1 and 2 of the East Asian lineage, which were identical to two haplotypes of ancient mtDNA (7,200 to 1,700 years before the present) excavated at archaeological sites in Okinawa. Conclusion: This study confirmed that the East Asian lineage is dominant in the maternal genetic background of the Agu population, supporting the hypothesis that the ancestors of the Agu pig were introduced from the Asian continent.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1484-1490
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• 2004

Mitochondrial DNA haplotypes from the displacement-loop (D-loop) region (436 bp) were genotyped and sequenced in Japanese Black beef cattle raised in the same herd. Correlation coefficients between mitochondrial DNA haplotypes, maternal lineage, birth weight, preweaning average daily gain, weaning weight, post weaning average daily gain and yearling weight were computed. The objective was to study the relationship between maternal and postnatal growth traits and to investigate if postnatal growth of calves to yearling age could be accurately predicted from mitochondrial DNA haplotypes. Results of the phylogenetic analysis revealed 17 maternal lineages and four mitochondrial DNA haplotypes. There were strong, positive and highly significant (p<0.001) correlations among maternal traits ranging from 0.52 to 0.98. Similarly, among postnatal growth traits, most of the correlations were also strong, positive and highly significant (p<0.001); the highest correlation of 0.94 was between preweaning average daily gain and weaning weight. However, correlations between mitochondrial DNA haplotypes and postnatal growth traits were very low, mostly negative and non-significant (p>0.05) ranging from -0.05 to 0.1. Prediction of postnatal growth from mitochondrial DNA yielded very low $R^{2}$ values ranging from 0.002 to 0.019. It was concluded that mitochondrial DNA polymorphism has no significant association with postnatal growth from birth to yearling age, and by implication, nuclear rather than cytoplasmic DNA, accounts for most of the genetic variation observed in postnatal growth of Japanese Black cattle. Therefore, mitochondrial DNA genotyping at an early age has no bearing on the accurate prediction of the future growth performance of calves.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.567-570
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• 1992

Variance components were estimated for calf daily gain from birth to 45 days of age in small (S), medium (M) and large (L) lines of beef cattle. Analyses involved records collected on 682 (S), 510 (M) and 228 (L) calves in Iowa, USA from 1978 to 1986. Cytoplasmic lines were determined based on the foundation female in the maternal lineage of each animal. Data were analyzed separately by size line using a derivative-free restricted maximum likelihood procedure under an animal model including additive direct (a), additive maternal (m), cytoplasmic lineage effects and covariance (a, m). The heritabilities for direct and maternal, and the cytoplasmic effects, were 0.13, 0.35 and 0.00 for S, 0.14, 0.32 and 0.00 for M, and 0.05, 0.33 and 0.03 for L. Genetic correlations (a, m) for S, M and L were -0.33, -0.57 and -1.00, respectively. The maternal genetic effect was the most important for calf growth between birth and 45 dyas of age and cytoplasmic variances were not important in any line.

• 대한가정학회지
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• 제34권4호
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• pp.357-371
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• 1996

This study explores the perspective of mothers who have children at teens concerning grandparents' role and grandchildren's role, especially focusing on the similarity and difference according to lineage. The analysis indicates as below. 1. Mothers highly expect the roles of grandparents which are related to the developing of self-identity of grandchildren at teens. There is much similarity between mothers; view on paternal grandparents' role and maternal grandparents' role. However the results support that paternal grandparents' role is more central than maternal grandparents in influencing to the development of self identity of grandchildren. 2. Grandchildren's role toward paternal grandparents is expected much more positively than grandchildrens' role toward maternal grandparents. 3. Mother's perspective on grandparents' role and grandchildren's role is associated with mother's view on the importance of grandparents in the lives of grandchildren.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.471-476
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• 2007

Little is known about the origin and genetic diversity of swamp buffaloes in China. To obtain more knowledge on genetics of the water buffalo in China, the complete mitochondrial D-loop sequences of 30 samples from 6 native types were investigated. The results revealed 12 mitochondrial haplotypes with 50 polymorphic sites. Among these polymorphic sites, there were 49 transitions and 1 transversion. The average nucleotide diversity and haplotype diversity estimated from mtDNA D-loop region in 6 Chinese water buffalo types were 0.00684 and 0.798, respectively, showing rather abundant mitochondrial genetic diversity. The Neighbor-Joining (NJ) tree of mtDNA of Chinese water buffaloes was constructed according to the 12 haplotypes. The NJ tree indicated two lineages being designated lineage A and lineage B, in which lineage A was predominant, and lineage B was at low frequency. The new lineage B was first discovered and defined in 6 Chinese water buffalo types. These results showed that two different maternal lineages were involved in the origin of domestic swamp buffaloes in China and the lineage B was probably an introgression from Southeast Asian buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.645-652
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• 2007

The origin of domestic donkeys in China has been controversial. To clarify the origin of Chinese domestic donkeys, we investigated the partial mitochondrial D-loop sequences of 126 samples from 12 native breeds. The results revealed two mitochondrial origins, lineage Somali and lineage Nubian of African wild ass detected in Chinese domestic donkeys. Lineage Somali was predominant in Chinese domestic donkey breeds. The pattern of genetic variation in ass mtDNA D-loop sequences indicated that the two lineages Somali and Nubian from China had undergone population expansion events. In a combined analysis of lineages Somali and Nubian between previously published sequences from other countries/regions and sequences of Chinese domestic donkeys, the results indicated that the two lineages of Chinese domestic donkeys were from Africa and supported the African maternal origins of Chinese domestic donkeys. There was no obvious geographical structure in Chinese domestic donkey breeds, but the population showed abundant mtDNA diversity. The spread routes of Chinese domestic donkeys were also discussed.

• 한국노년학
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• 제34권2호
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• pp.277-297
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• 2014

본 연구는 청년기 손자녀의 인식을 기초로 조손간의 유대관계를 이해하는 것에 목적을 두었으며 손자녀 성(손자, 손녀)과 조부모 혈족(친조부모, 외조부모)에 따라 구분하여 조손관계를 분석하였다. 연구대상은 조부모님이 적어도 한 분 이상 생존해 계시는 만 19~25세의 청년세대 501명을 대상으로 자기보고식 설문지를 통해 평가하였다. 손자녀와 조부모간의 직접적 관계에 기초한 주요 특성변인들이 손자녀 성에 따라 차이가 있는지 t검증을 실시하고, 조손유대에 대한 부모특성변인들과 개인특성변인들의 설명력을 확인하기 위해 위계적 회귀분석을 실시하였으며, 그 결과는 다음과 같다. 첫째, 손자녀 성에 따라 친조부모와의 가치유사성, 친조부모와의 유대, 외조부모와의 접촉에서 유의미한 차이가 나타났다. 둘째, 지각된 아버지-조부모 관계 질, 어머니-조부모 관계 질, 접촉, 가치유사성은 모든 조손관계에서 조손유대를 유의미하게 예측하였으나, 각 특성변인의 상대적인 설명력은 각 조손관계에 따라 다양한 양상으로 나타나 조손유대를 설명하는 메커니즘이 손자, 손녀와 부계, 모계에 따라 다름을 보여주었다. 이러한 결과에 기초한 한국문화적 맥락에 따른 함의와 후속연구를 위한 제언을 논의에 제시하였다.

• 식물분류학회지
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• 제49권4호
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• pp.282-293
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• 2019

Broussonetia × kazinoki Siebold has long been utilized as a major component in the manufacturing of Korean traditional paper, hanji, and has been suggested as a hybrid species of B. papyrifera and B. monoica. By applying three molecular markers, chloroplast (cp) ndhF-rpl32 IGS, a nuclear ribosomal internal transcribed spacer, and the TOPO6 gene, the hybrid origin of B. × kazinoki is tested. As a result, B. × kazinoki in Korea is demonstrated to be a hybrid of B. monoica × B. papyrifera, most likely formed naturally in Korea. The cp haplotypes detected provided information about the origins and genetic diversity of the maternal lineage B. monoica and paternal lineage B. papyrifera. The two nuclear markers were supplemented to each other, leading to the discovery of introgression in Broussonetia.

• 한국조사연구학회지:조사연구
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• 제13권1호
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• pp.89-112
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• 2012

본 연구는 세대간 애정적 결속의 양상과 결정요인을 부계와 모계로 구분하여 분석하고자 하였다. 세대간의 경제적, 도구적 정서적 지원을 통한 기능적 결속이나 잦은 만남 내지 연락을 통한 교류적 결속 거주지의 근접성을 바탕으로 한 구조적 결속의 양상과 변화에 대한 연구들과 달리 세대간 친밀감을 나타내는 애정적 결속에 대한 연구는 부족한 편이었다. 본 연구에서는 손자녀가 조부모에 대해 느끼는 정서적 친밀감을 종속변수로 하여 애정적 결속이 어떻게 나타나는지 살펴보고자 하였다. 또한 앞서 언급한 기능적, 교류적, 구조적 결속같은 요인들이 어떻게 애정적 결속에 영향을 미치는지를 살펴보고자 하였다. 이를 위하여 2011년 7월에 조사된 세대별 문화경험과 문화인식에 관한 설문조사에서 자녀가 있는 기혼이면서 양가 부모중 적어도 한분씩은 생존해 있는 30~59세 응답자 330명을 대상으로 분석하였다. 방법론으로는 '양가 조부모에게 느끼는 친밀감이 같다'라는 응답을 준거집단으로 삼은 다항로짓회귀분석을 사용하여 부계와 모계간에 변수별 영향력이 어떻게 다르게 나타나는지 보았다. 그 결과 부계에서는 경제적 지원 관계가 애정적 결속을 높이는 주요한 요인으로 드러난 반면, 모계에서는 경제적 지원뿐 아니라 도구적, 정서적 지원도 주요한 요인으로 작용하고 있음을 확인할 수 있었다.

• 생명과학회지
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• 제21권3호
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• pp.341-348
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• 2011

제주재래돼지의 모계 혈통에 대한 보다 명확한 이해를 얻기 위해, 본 연구에서는 제주재래돼지의 미토콘드리아 DNA (mtDNA) CYTB 유전자를 분석하고 이를 타 품종들에서 얻은 결과들과 비교하였다. 제주재래돼지를 포함한 돼지 6 품종에서 PCR-RFLP 분석을 수행하였고, RFLP 양상은 돼지 품종들을 뚜렷하게 구분되는 두 가지 반수체형(mtCYTB1 and mtCYTB2)으로 분리시켰다. 제주재래돼지 CYTB 서열들은 계통수 상에서 유럽과 아시아품종 cluster에서 모두 발견되었다. 제주재래돼지 CYTB들 중에서 J2 group은 중국재래돼지품종들과 근연이면서 아시아 고유 돼지 계통들과 함께 출현하였으며, 다른 한 group인 J1에 해당하는 서열들은 유럽돼지 계통들과 함께 위치하였고, 아시아 품종들보다는 스페인의 Iberian 재래돼지들과 근연인 것으로 확인되었다. 이 결과들은 현재 제주도에서 사육되고 있는 제주재래돼지 품종의 모계 기원은 크게 아시아계 돼지와 유럽계 돼지인 것으로 추정됨을 보여준다. 따라서 본 연구결과들은 제주재래돼지 집단은 과거에 가축화된 아시아 고유 돼지품종들과 공통 선조를 공유하고, 또한 20세기에 유입된 유럽계 돼지 품종들도 현재의 집단 형성에 기여한 것임을 시사하고 있다.