• 대한예방의학회:학술대회논문집
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• 1999.10a
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• pp.1-15
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• 1999

In a case-control study to evaluate the factors involved in the development of hepatocellular carcinoma, polymorphisms of the p53 gene were compared in 68 cases mostly infected with hepatitis C virus (HCV) and 68 controls matched for sex and age: DNA from peripheral blood leukocytes was analyzed by the polymerase chain reaction-single strand conformation polymorphism method and direct sequencing. Polymorphisms analyzed were those in exon 4 (CCC vs. CGC, Pro vs. Arg at codon 72, Al allele vs. A2 allele), intron 2 (C vs. G at nucleotide 38, Al vs. A2), intron 3 (C vs. A at nucleotide 65, Al vs. A2; absence and presence of 16 base pair repeat at nucleotides 24 to 39, Al vs. A2), intron 6 (A vs. G at nucleotide 62, Al vs. A2) and intron 7 (C and T vs. T and G at nucleotides 72 and 92, Al vs. A2). A significantly higher frequency of the allele for CCC (Pro, Al) at codon 72 of exon 4 was found in cases (39%) than in controls (26%) (p<0.05). Highly significant linkage of the polymorphisms in exon 4, intron 2, intron 3 and intron 7, and between the intron 3-16 bp duplication and polymorphism in intron 6 also was found. Matched Fair analysis showed significantly higher frequencies of certain haplotypes (1-1-1-1-2-2 or 1-1-2-1-2-1 for exon 4, intron 2, intron 3, the intron 3-16 bp duplication, intron 6 and intron 7) in cases than in controls (p=0.014, OR=2.27, 95% CI= 1.08-5.12). No preference of specific p53 polymorphisms for specific HCV genotype was detected. These findings suggest that in hepatocarcinogenesis mainly due to HCV infection, genetic factors may be involved and that genetic markers can serve as predictors of a high-risk group for hepatocarcinogenesis.

• Journal of Digital Convergence
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• v.11 no.10
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• pp.737-746
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• 2013

This research aim is to investigate the rate and pattern of re-using the four-digit personal identification numbers(PINs). 1313 types of PINs were observed by 224 students who took this author's classes from last 2006 to 2011 at A-university. Some students used PINs as few as 3-4 and as many as 12-13. The average is 5.86 per person. The rates of re-using PINs were calculated by each student. 87%(195/224) of students reused PINs and 64% of them reused with just only one type of PINs, 20% reused with 2 types, and about 3% reused with 3-4 types. With respect to PINs, 884 out of the total 1313 PINs were reused, that is around 64.3%. In a broad sense, a pair of slight modification of PINs were also observed, that is, new PINs were partly matched in position or size of numbers of previous PINs. And if the reuse rate falling under the slight modification of PINs, 10.4% is added, about 75% of the PINs were reused in a broad sense. The re-using rate of male students is higher than the one of female students. This paper's results may provide to make plan for hacks of passwords.

• Journal of Korean Medicine Rehabilitation
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• v.21 no.4
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• pp.205-217
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• 2011

Objectives: This study was conducted to identify the effects of the Daoyin and corrective exercise program on musculoskeletal pain among acute sprain patients due to traffic accidents. Methods: This study was carried out on the 35 patients who had been treated on musculoskeletal pain due to traffic accidents and was performed from March 1 to June 31, 2011 in the department of oriental rehabilitation medicine, Dun-San oriental hospital attached Dae-Jeon university. And we divided those 35 patients into 2 groups; sample group took treated with the Daoyin and corrective exercise program control group took common treatment only. All of the subjects were examined en visual analogue scale(VAS), state-trait anxiety inventory(STAI) score and presence of treatment after hospital discharge. The data was collected before and after the treatment, after hospital discharge. SPSS ver. 12.0 for Windows was used fer data analysis, and the analysis, and the general characteristics were demonstrated by frequency number and two-independent sample t-test. The effects of the Daoyin and corrective exercise program were analyzed by matched pair t-test and analysis of covariance. Results: On the VAS score, there was significant difference between before and after treatment, and before and after hospital discharge. And on the VAS scores, sample group mowed higher significant change of VAS than control group. And on the STAI score, there was significant difference between before and after treatment. But it did not show statistical significance between sample and control group. Conclusions: The Daoyin and corrective exercise program is an effective treatment fer musculoskeletal pain among acute sprain patients due to traffic accidents.

• BMB Reports
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• v.51 no.11
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• pp.584-589
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• 2018

Secondary prevention via earlier detection would afford the greatest chance for a cure in premalignant lesions. We investigated the exomic profiles of non-malignant and malignant changes in head and neck squamous cell carcinoma (HNSCC) and the genomic blueprint of human papillomavirus (HPV)-driven carcinogenesis in oropharyngeal squamous cell carcinoma (OPSCC). Whole-exome (WES) and whole-genome (WGS) sequencing were performed on peripheral blood and adjacent non-tumor and tumor specimens obtained from eight Korean HNSCC patients from 2013 to 2015. Next-generation sequencing yielded an average coverage of $94.3{\times}$ for WES and $35.3{\times}$ for WGS. In comparative genomic analysis of non-tumor and tumor tissue pairs, we were unable to identify common cancer-associated early mutations and copy number alterations (CNA) except in one pair. Interestingly, in this case, we observed that non-tumor tonsillar crypts adjacent to HPV-positive OPSCC appeared normal under a microscope; however, this tissue also showed weak p16 expression. WGS revealed the infection and integration of high-risk type HPV16 in this tissue as well as in the matched tumor. Furthermore, WES identified shared and tumor-specific genomic alterations for this pair. Clonal analysis enabled us to infer the process by which this transitional crypt epithelium (TrCE) evolved into a tumor; this evolution was accompanied by the subsequent accumulation of genomic alterations, including an ERBB3 mutation and large-scale CNAs, such as 3q27-qter amplification and 9p deletion. We suggest that HPV16-driven OPSCC carcinogenesis is a stepwise evolutionary process that is consistent with a multistep carcinogenesis model. Our results highlight the carcinogenic changes driven by HPV16 infection and provide a basis for the secondary prevention of OPSCC.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.32 no.5
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• pp.459-467
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• 2014

The importance of fusion and association using established spatial information has increased gradually with the production and supply of various spatial data by public institutions. The generation of necessary spatial information without field investigation and additional surveying can reduce time, labor, and financial costs. However, the study of the integration of the newly introduced road name address map with the digital map is very insufficient. Even though the use of the road name address map is encouraged for public works related to spatial information, the digital map is still widely used because it is the national basic map. Therefore, in this study, building matching and update were performed to associate the digital map with the road name address map. After geometric calibration using the block-based ICP (Iterative Closest Point) method, multi-scale corresponding pair searching with hierarchical clustering was applied to detect the multi-type match. The accuracy assessment showed that the proposed method is more than 95% accurate and the matched building layer of the two maps is useful for the integrated application and fusion. In addition, the use of the road name address map, which carries the latest and most frequently renewed data, enables cost-effective updating of new buildings.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8893-8900
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• 2014

Dysregulated expression of microRNAs (miRNAs) has been shown to be closely associated with tumor development, progression, and carcinogenesis. However, their clinical implications for gastric cancer remain elusive. To investigate the hypothesis that genome-wide alternations of miRNAs differentiate gastric cancer tissues from those matched adjacent non-tumor tissues (ANTTs), miRNA arrays were employed to examine miRNA expression profiles for the 5-pair discovery stage, and the quantitative real-time polymerase chain reaction (qRTPCR) was applied to validate candidate miRNAs for 48-pair validation stage. Furthermore, the relationship between altered miRNA and clinicopathological features and prognosis of gastric cancer was explored. Among a total of 1,146 miRNAs analyzed, 16 miRNAs were found to be significantly different expressed in tissues from gastric cancer compared to ANTTs (p<0.05). qRT-PCR further confirmed the variation in expression of miR-193b and miR-196a in the validation stage. Down-expression of miR-193b was significantly correlated with Lauren type, differentiation, UICC stage, invasion, and metastasis of gastric cancer (p<0.05), while over-expression of miR-196a was significantly associated with poor differentiation (p=0.022). Moreover, binary logistic regression analysis demonstrated that the UICC stage was a significant risk factor for down-expression of miR-193b (adjusted OR=8.69; 95%CI=1.06-56.91; p=0.043). Additionally, Kaplan-Meier survival curves indicated that patients with a high fold-change of down-regulated miR-193b had a significantly shorter survival time (n=19; median survival=29 months) compared to patients with a low fold-change of down-regulated miR-193b (n=29; median survival=54 months) (p=0.001). Overall survival time of patients with a low fold-change of up-regulated miR-196a (n=27; median survival=52 months) was significantly longer than that of patients with a high fold-change of up-regulated miR-196a (n=21; median survival=46 months) (p=0.003). Hence, miR-193b and miR-196a may be applied as novel and promising prognostic markers in gastric cancer.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.3
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• pp.305-315
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• 2010

This research aims to develop a method to determine the 3D coordinates of an object point from overlapping omni-directional images acquired by a ground mobile mapping system and assess their accuracies. In the proposed method, we first define an individual coordinate system on each sensor and the object space and determine the geometric relationships between the systems. Based on these systems and their relationships, we derive a straight line of the corresponding object point candidates for a point of an omni-directional image, and determine the 3D coordinates of the object point by intersecting a pair of straight lines derived from a pair of matched points. We have compared the object coordinates determined through the proposed method with those measured by GPS and a total station for the accuracy assessment and analysis. According to the experimental results, with the appropriate length of baseline and mutual positions between cameras and objects, we can determine the relative coordinates of the object point with the accuracy of several centimeters. The accuracy of the absolute coordinates is ranged from several centimeters to 1 m due to systematic errors. In the future, we plan to improve the accuracy of absolute coordinates by determining more precisely the relationship between the camera and GPS/INS coordinates and performing the calibration of the omni-directional camera

• Journal of Digital Convergence
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• v.16 no.12
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• pp.87-95
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• 2018

The purpose of this study was to validate the effectiveness of academic motivation improvement program for underachieved students. Participants in the program consisted of 14 sophomores and senior. They were randomly assigned to either the experimental group(n=7) or the control group(n=7). To analysis the effectivness, matched pair t-test and independent t-test. The programs offered 6times. The results are follows. First, for the experimental group that took part in motivation improvement program, there was a increase in self-esteem. But the improvement was not significant. Second, for the experimental group that took part in motivation improvement program, there was a significant decrease in academic amotivation. Third, the experimental group showed a more significant decrease in academic amotivation. Finally, the significance and limitations of this study and suggestions for future research were presented.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.151-159
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• 2002

Genetic map and molecular marker have a great importance in improving and facilitating crop breeding program as well as in genome analysis and map-based cloning of genes representing desirable characters. This study aimed at developing RAPD markers and constructing a genetic linkage map using 82 BC$_1$F$_1$individuals originated from the cross between '835' and B$_2$in radish (Raphanus sativus L.). One of the parents for genetic linkage map construction, '835'(P$_1$) of egg type is susceptible to Fusarium wilt and have medium resistance to virus infection and the other parent, B$_2$(P$_2$) of round type, is susceptible to Fusarium wilt and virus, Screening of 394 RAPD primers in BC$_1$F$_1$) population resulted in selecting 128 polymorphic markers which displayed 1:1 segregation pattern. Two markers failed to display 1:1 segregation and showed the segregation ratio skewed to maternal genotype. Selected markers were categorized into 14 linkage group based on LOD score represented by MAPMAKER/EXP program. Five groups composed of single marker among them were excluded from the linkage map, and consequently, the remaining groups are well matched with the number of radish chromosome (n＝9). The linkage map constructed with 128 markers covers 1,688.3 cM and the average distance between markers was 13.8 cM. For developing STS marker, we determined the partial nucleotide sequence of OPE10 marker at both ends and designed a oligonucleotide primer pair based on this sequence. STS PCR using the primer pair displayed a single, clear band of which segregation is perfectly matched with that of OPE10 marker. This implies that RAPD markers could readily convert into clear and reliable STS markers.

• Journal of Korean Medical Science
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• v.33 no.43
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• pp.277.1-277.10
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• 2018

Background: To examine survival rates and renal function after partial nephrectomy (PN) and radical nephrectomy (RN) in patients with chronic kidney disease (CKD). Methods: We studied 4,332 patients who underwent PN or RN for pathological T1a-T2N0M0 renal cell carcinoma from 1988 to 2014. Patients were divided into two subgroups of CKD stage I-II and stage III. Kidney function, and survival outcomes were compared between groups. Results: We included 1,756 patients with CKD I-II and 276 patients with CKD III in the final pair-matched analysis. Kidney function was significantly better preserved in the PN than in the RN group among all patients. However, the beneficial effect of PN on kidney function gradually disappeared over time in CKD III patients. The 5-year overall survival (OS) rates after PN and RN differed in patients with CKD I-II disease (99.4% vs. 96.5%, respectively, P = 0.015). The 5-year OS rates after surgery were not affected by mode of nephrectomy in CKD III patients (97.8% vs. 93.5%, P = 0.103). The 5-year cancer-specific survival rates did not differ between treatment groups in all CKD stage. Cox hazard analysis showed that the operative method was a significant factor for OS in CKD I-II patients (hazard ratio [HR], 0.320; confidence interval [CI], 0.122-0.840; P = 0.021). However, PN was not beneficial in terms of OS in CKD III patients (HR, 0.395; CI, 0.086-1.172; P = 0.117). Conclusion: PN is associated with a higher OS rate and better kidney function in patients with preoperative CKD stage I and II, but not in those with CKD stage III.