• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2018년도 춘계 학술논문 발표대회
• /
• pp.255-256
• /
• 2018

In this study, to confirm the performance of gel type urethane (hereinafter referred to as urethane gel) developed for the purpose of securing waterproof stability after application by increasing compatibility with asphalt mastic film material, general urethane coating material and adhesion. As a result, gel type urethane showed "all right" in fatigue resistance test.

• 한국식품영양과학회지
• /
• 제43권10호
• /
• pp.1500-1509
• /
• 2014

본 연구는 위 벽세포 in vitro 실험모델과 알코올로 위를 자극한 in vivo 실험모델을 이용하여 매스틱 검의 위산 분비 억제 및 위장 점막 보호 효과를 확인하고자 하였다. 위 조직의 병리학적인 관찰을 통하여 효과를 관찰한 결과 알코올로 위를 자극한 군에서 위 점막 조직의 손상과 표면 상피세포의 손실을 관찰할 수 있었으나, 매스틱 검 50 및 100 mg/kg 투여한 군에서 모두 control 그룹과 비교했을 때 손상이 회복되었음을 확인할 수 있었다. 위액 분비량 및 위액 산도를 비교한 결과에서도 알코올의 자극으로 인하여 증가한 위액 분비량과 위액 산도를 매스틱 검이 유의적으로 감소시켰음을 확인할 수 있었다. 혈장 histamine 농도와 그로 인해 영향을 받은 H2r 발현량 변화로 위산 분비 관련 인자를 확인한 결과에서도 매스틱 검을 투여한 그룹에서 유의적으로 그 농도가 감소한 것을 확인하였다. 또한 위 벽세포에서 위산 분비 증가와 관련된 수용체인 CCK2r과 $H^+/K^+$ APTase 발현 변화도 역시 매스틱 검의 투여가 효과적으로 발현을 억제한 것으로 나타났다. 이러한 동물실험 결과를 바탕으로 세포 수준에서의 기전 규명을 위한 연구를 추가적으로 진행하였으며, 세포 내 cAMP 농도, $H^+/K^+$ APTase 및 H2r의 발현 변화를 관찰한 결과 매스틱 검의 처리가 효과적으로 이들의 발현을 감소시켰음을 확인하였다. 따라서 매스틱 검의 위산 분비 억제 및 점막 보호 활성 효과는 형태학적 및 병리학적 관찰, histamine 분비 억제, 수용체 발현 억제효과 등으로 확인한 결과, histamine을 통한 위산 분비 경로를 조절함으로써 위산 분비 및 위장 점막 손상에 대해 보호 효과를 나타내었으며 이는 이전 실험 결과들과 동일한 농도에서 효과가 나타났음을 확인하였다.

• International Journal of Oral Biology
• /
• 제36권1호
• /
• pp.13-21
• /
• 2011

Chios gum mastic (CGM) is produced from Pistiacia lentiscus L var chia, which grows only on Chios Island in Greece. CGM is a kind of resin extracted from the stem and leaves, has been used for many centuries in many Mediterranean countries as a dietary supplement and folk medicine for stomach and duodenal ulcers. CGM is known to induce cell cycle arrest and apoptosis in some cancer cells. This study was undertaken to investigate the alteration of the cell cycle and induction of apoptosis following CGM treatment of HL-60 cells. The viability of the HL-60 cells was assessed using the MTT assay. Hoechst staining and DNA electrophoresis were employed to detect HL-60 cells undergoing apoptosis. Western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses were also employed. CGM treatment of HL-60 cells was found to result in a dose- and time-dependent decrease in cell viability and apoptotic cell death. Tested HL-60 cells showed a variety of apoptotic manifestations and induced the downregulation of G1 cell cycle-related proteins. Taken collectively, our present findings demonstrate that CGM strongly induces G1 cell cycle arrest via the modulation of cell cycle-related proteins, and also apoptosis via proteasome, mitochondrial and caspase cascades in HL-60 cells. Hence, we provide evidence that a natural product, CGM could be considered as a novel therapeutic for human leukemia.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제35권2호
• /
• pp.125-130
• /
• 2009

For many years, intermaxillary fixation using arch bar has been operated in treatment of mandibular fracture patients. But it has many complications including injury of operators and assistants cause by wire, inflammation of periodontium. For that reasons alternatives are required; osteosynthesis technique using mini plate, intermaxillary fixation using IMF screws have been available. Treatment by arch bar fixation, however, is still valuable to treat craniomaxillary fracture patients. The purpose of this study is to know effect arch bar on periodontium and influence gingival gel on periodontium applied by arch bar. 40 mandibular fracture patients are monitored. 30 patients were applied by arch bar, 10 patients were not. And the former were classified by 3 categories; Nano vitamin and Mastic gel were applied to 10 patients respectively and any gingival gel was not used to 10 patients. Clinical attachment level, bleeding on probing and periodontal depth of each group were measured and compared before operation and on 2 weeks and 6 weeks after operation. Mann-Whitney U test was used to analyze result which leads to this conclusion. 1. Whether arch bar is applied or not, treatment of mandlbular fracture gave rise to gingivitis, but 6 weeks after operation, gingivitis is restored to the same level as the state before operation. 2. More severe gingivitis appeared when arch bar is applied to mandibular fracture than when it is not. 3. Both gingival gel used in this study can reduce gingivitis which can be caused by arch bar. 4. In this study, Mastic gel is more effective for prevent gingival inflammation cause by arch bar than nano vitamin. In regard to this result, gingivitis is considered to be available because it is reversible and does not induce periodontal disease. Gingival gel is regarded to be helpful for patients applied by arch bar to feel less discomfort.

• International Journal of Oral Biology
• /
• 제38권3호
• /
• pp.101-110
• /
• 2013

We investigated the synergistic apoptotic effects of co-treatments with Chios gum mastic (CGM) and eugenol on G361 human melanoma cells. An MTT assay was conducted to investigate whether this co-treatment efficiently reduces the viability of G361 cells compared with each single treatment. The induction and augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining, and analyses of DNA hypoploidy. Western blot analysis and immunofluorescent staining were also performed to evaluate expression and translocation of apoptosis-related proteins following CGM and eugenol co-treatment. Proteasome activity and mitochondrial membrane potential (MMP) changes were also assayed.The results indicated that the co-treatment of CGM and eugenol induces multiple pathways and processes associated with an apoptotic response in G361 cells. These include nuclear condensation, DNA fragmentation, a reduction in MMP and proteasome activity, an increase of Bax and decrease of Bcl-2, a decreased DNA content, cytochrome c release into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, and the activation of caspase-9, caspase-7, caspase-3, PARP and DFF45 (ICAD). In contrast, separate treatments of $40{\mu}g/ml$ CGM or $300{\mu}M$ eugenol for 24 hours did not induce apoptosis. Our present data thus suggest that a combination therapy of CGM and eugenol is a potential treatment strategy for human melanoma.

• International Journal of Oral Biology
• /
• 제39권2호
• /
• pp.65-73
• /
• 2014

Chios Gum Mastic (CGM) is a natural resin extracted from the leaves of Pistacia lentiscus, a plant endemic to the Greek island of Chios. It has been used by traditional healers, and it has antibacterial, antifungal properties, and therapeutic benefits for the skin. The CGM reduces the formation of dental plaque and bacterial growth in oral saliva, and recent studies have demonstrated the role of antioxidant activity of CGM. Although CGM has been widely investigated, its protective effect against oxidative-damage to keratinocytes, as well as the relationship between CGM and autophagy, has not been investigated. The aim of this study was to assess the protective effect of CGM against $H_2O_2$-induced oxidative stress and to evaluate the autophagic features induced by CGM in human keratinocytes. The pretreatment with CGM significantly reduced apoptosis in $H_2O_2$-exposed HaCaT cells. It promoted the degradation of caspase-3, caspase-8, and caspase-9; and it induced the formation of the processed PARP. The treatment with CGM caused an increase in vesicle formation compared to control group. The level of p62 was reduced and the conversion of LC3-I to LC3-II was increased in CGM treated HaCaT cells. Also, the treatment with CGM increased cleavage of ATG5-ATG12 complex. In summary, CGM helps the cells to survive under stressful conditions by preventing apoptosis and enhancing autophagy. Besides, the present investigation provides evidence to support the antioxidant potential of CGM in vitro and opens up a new horizon for future experiments.

• 한국건축시공학회지
• /
• 제18권4호
• /
• pp.337-345
• /
• 2018

본 연구에서는 누유 현상에 대한 원인 파악을 위하여 실제 현장에서 누유된 시료를 채취하여 육안 관찰을 실시한 결과, 표면 상태의 변화를 근거로 누유 현상 원인이 "구성 성분간 분리"로 판단하고, 이에 대한 검증을 실시하였다. 검증을 위하여 현장에서 많이 사용되고 있는 아스팔트 매스틱 도막재 3개 제품을 수직 실린더에 채워 $50{\pm}5^{\circ}C$의 챔버 속에 정치시킨 후, 1일부터 28일간 매일 실린더의 상부, 하부 재료를 대상으로 Filler 함량차를 측정하여, 변화율을 비교 분석하였다. 평가 결과, 정치 시일이 경과할수록 아스팔트 매스틱의 Filler 성분이 침강되는 분리현상을 확인할 수 있었으며, 정치 후 28일에는 상부와 하부 시료의 Filler 함량이 최대 23.8% 이상 차이가 나는 것을 확인할 수 있었다. 상기 결과를 근거로 아스팔트 매스틱의 누유 발생 메커니즘에 대한 가설 수립 후 누유발생 전 과정에 대한 예측 모델을 제시하였다.

• 대한치과의사협회지
• /
• 제17권10호통권125호
• /
• pp.769-772
• /
• 1979

Child female 11 years old fractures of mandible in symphysis and left condyle head, conservative treated intermaxillary wiring (Rubber bands elastic) 8 days after mandible movement fractures healed. Patient has practically normal mastic atory function and no complaints.

• 한국도로학회논문집
• /
• 제15권1호
• /
• pp.103-110
• /
• 2013

PURPOSES: The objective of this study is to analyze and evaluate the behavior of orthotropic steel bridge deck pavement using three-dimensional finite element analysis and full-scale wheel load testing. METHODS: Since the layer thickness and material properties used in the bridge deck pavement are different from its condition, it is very difficult to measure and access the behavior of bridge deck pavement in the field. To solve this problem, the full-scale wheel load testing was conducted on the PSMA/Mastic bridge deck pavement and the deflection of bridge deck and horizontal tensile strain on top of pavement were measured under the loading condition. Three-dimensional finite element analysis was conducted to predict the behavior of bridge deck pavement and the predicted deflection and tensile strain values are compared with measured values from the wheel loading testing. RESULTS: Test results showed that the predicted deflections are 10% lower than measured ones and the error between predicted and measured horizontal tensile strain values is less than 2% in the critical location. CONCLUSIONS: The fact indicates that the proposed the analysis is found to be accurate for estimating the behavior of bridge deck pavements.

• 한국도로학회논문집
• /
• 제18권1호
• /
• pp.91-97
• /
• 2016

PURPOSES : The objective of this paper is to develop a pavement performance model based on the Bayesian algorithm, and compare the measured and predicted performance data. METHODS : In this paper, several pavement types such as SMA (stone mastic asphalt), PSMA (polymer-modified stone mastic asphalt), PMA (polymer-modified asphalt), SBS (styrene-butadiene-styrene) modified asphalt, and DGA (dense-graded asphalt) are modeled in terms of the performance evaluation of pavement structures, using the Bayesian algorithm. RESULTS : From case studies related to the performance model development, the statistical parameters of the mean value and standard deviation can be obtained through the Bayesian algorithm, using the initial performance data of two different pavement cases. Furthermore, an accurate performance model can be developed, based on the comparison between the measured and predicted performance data. CONCLUSIONS : Based on the results of the case studies, it is concluded that the determined coefficients of the nonlinear performance models can be used to accurately predict the long-term performance behaviors of DGA and modified asphalt concrete pavements. In addition, the developed models were evaluated through comparison studies between the initial measurement and prediction data, as well as between the final measurement and prediction data. In the model development, the initial measured data were used.