• Title/Summary/Keyword: Massachusetts Institute of Technology

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Vegetative Incompatibility and Hypovirulence Conversion of Cryphonectria(Endothia) parasitica(Murr.)Barr (Cryphonectria(Endothia) parasitica(Murr.)Barr의 체세포(體細胞) 불화합성(不和合性)과 저병원성(低病原性) 변환(變換))

  • Lee, Jong-Kyu
    • The Korean Journal of Mycology
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    • v.22 no.4
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    • pp.343-349
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    • 1994
  • One hundred and two virulent(V) strains of Cryphonectria parasitica were isolated from the cankers of American chestnut (Castanea dentata) trees in western Massachusetts, USA. The diversity of vegetative compatibility groups (VCGs) of C. parasitica was investigated. One hundred and two strains represented 54 VCGs; 38 VCGs had only one strain each, 6 VCGs had 2 strains each, and 10 most common VCGs had 52 strains. Great diversity in VCGs may due to the increasing numbers of VCGs with time since the pathogen has been in Massachusetts for 80 years. Ten vegetative compatibility representative strains were selected from the 10 most common VCGs and converted to hypovirulent (H) strains through the pairing and hyphal anastomosis of H strains (4 strains with French dsRNA elements and 17 strains with Italian dsRNA elements). All of the 10 representative strains were converted to H strains by at least more than one of the H strains.

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Double-walled carbon nanotubes: synthesis, structural characterization, and application

  • Kim, Yoong Ahm;Yang, Kap-Seung;Muramatsu, Hiroyuki;Hayashi, Takuya;Endo, Morinobu;Terrones, Mauricio;Dresselhaus, Mildred S.
    • Carbon letters
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    • v.15 no.2
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    • pp.77-88
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    • 2014
  • Double walled carbon nanotubes (DWCNTs) are considered an ideal model for studying the coupling interactions between different concentric shells in multi-walled CNTs. Due to their intrinsic coaxial structures they are mechanically, thermally, and structurally more stable than single walled CNTs. Geometrically, owing to the buffer-like function of the outer tubes in DWCNTs, the inner tubes exhibit exciting transport and optical properties that lend them promise in the fabrication of field-effect transistors, stable field emitters, and lithium ion batteries. In addition, by utilizing the outer tube chemistry, DWCNTs can be useful for anchoring semiconducting quantum dots and also as effective multifunctional fillers in producing tough, conductive transparent polymer films. The inner tubes meanwhile preserve their excitonic transitions. This article reviews the synthesis of DWCNTs, their electronic structure, transport, and mechanical properties, and their potential uses.

Design and Simulation of Integral Twist Control for Helicopter Vibration Reduction

  • Shin, Sang-Joon;Cesnik Carlos E. S.;Hall Steven R.
    • International Journal of Control, Automation, and Systems
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    • v.5 no.1
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    • pp.24-34
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    • 2007
  • Closed-loop active twist control of integral helicopter rotor blades is investigated in this paper for reducing hub vibration induced in forward flight. A four-bladed fully articulated integral twist-actuated rotor system has been designed and tested successfully in wind tunnel in open-loop actuation. The integral twist deformation of the blades is generated using active fiber composite actuators embedded in the composite blade construction. An analytical framework is developed to examine integrally twisted helicopter blades and their aeroelastic behavior during different flight conditions. This aeroelastic model stems from a three-dimensional electroelastic beam formulation with geometrical-exactness, and is coupled with finite-state dynamic inflow aerodynamics. A system identification methodology that assumes a linear periodic system is adopted to estimate the harmonic transfer function of the rotor system. A vibration minimizing controller is designed based on this result, which implements a classical disturbance rejection algorithm with some modifications. Using the established analytical framework, the closed-loop controller is numerically simulated and the hub vibratory load reduction capability is demonstrated.

Templated solid-state dewetting of thin films

  • Ye, Jong-Pil;Thompson, Carl V.;Giermann, Amanda L.
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2012.05a
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    • pp.54.2-54.2
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    • 2012
  • Solid-state dewetting of thin films is a process through which continuous solid films decay to form islands. Dewetting of thin films has long been a critical issue in microelectronics and much effort has been made to prevent the process and enhance the stability of films. On the other hand, dewetting has also been purposely induced to create arrays of particles and other structures for applications, including plasmonic structures and catalysts for growing nanotube and nanowire. We have investigated ways of producing regular structures via templated dewetting of thin films. Mainly, two different approaches have been used in our works to template dewetting of thin films: periodic topographical templating and planar patterning of epitaxially-grown films. Dewetting of topographically-patterned thin films results in the formation of nanoparticle arrays with spatial and crystallographic orders. Morphological evolution during templated-dewetting of single crystal films occurs in deterministic ways because of geometric and crystallographic constraints, and leads to the formation of regular structures with smaller sizes and more complex shapes than the initial patches. These results will be reviewed in this presentation.

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Salting-out Effects on the Partition of Proteins in Aqueous Two-phase Systems

  • KIM, CHAN-WHA;CHO KYUN RHA
    • Journal of Microbiology and Biotechnology
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    • v.6 no.5
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    • pp.352-357
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    • 1996
  • The partition of proteins in the salt-rich phase of polyethylene glycol (pEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out con-stant) for a given protein is determined by the type of salt in the two-phase systems.

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Systematic Study of Fluorescein-Functionalized Macrophotoinitiators for Colorimetric Bioassays

  • Lee, Jeong-Gyu;Han, Gyeong-Yeop;Go, Sang-Won;Sikes, Hadley D.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.263.2-263.2
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    • 2013
  • We report a systematic investigation of a set of macrophotoinitiators for use in polymerization-based signal amplification. To test the dependence of photopolymerization responses on the number of photoinitiators localized per molecular recognition event, we gradually increased the number of photoinitiator molecules coupled to a scaffold macromolecule. Macrophotoinitiators constructed with an average of 7 to 168 photoinitiators per polymer with the goals of quantifying the relationship between the number of initiators per binding event and the degree of amplified colorimetric readout. To evaluate the capacity of the macrophotoinitiators to detect molecular recognition, neutravidin was coupled to these molecules to recognize biotin-labeled DNA immobilized on biochip test surfaces. Fluorescein macroinitiators are found to be useful in detecting molecular recognition above a threshold of initiators per polymer. Above this threshold, increasing the number of initiators per macroinitiator resulted in increased signal strength.

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Enhancement of the Escherichia coli Floc Strength with Water Soluble Polymers

  • KIM, CHAN-WHA;CHOKYUN RHA
    • Journal of Microbiology and Biotechnology
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    • v.7 no.4
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    • pp.282-286
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    • 1997
  • The floc strength of Escherichia coli was enhanced by adding water soluble polymer flocculants (BPA-5020 and BPA-5000) to the particulate flocculant (BPA-1000) as indicated by the increase in the shear index. The shear index of the E. coli flocs increased from 0.39 with the particulate flocculant alone to 0.94 with the particulate flocculant in conjunction with the water soluble polymer flocculant. In addition, the sedimentation rate of flocs was higher and the sedimented volume of flocs was smaller when the particulate flocculant was used with the water soluble polymer flocculant. When E. coli was flocculated first with the water soluble flocculant and the particulate flocculant was added later into the E. coli flocs formed, the sedimentation rate of the flocs was greater than that of any other combination. The shear index of the flocs was, however, independent of the sequence of the flocculant addition.

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Experimental study on the heat flux and heat transfer coefficient in a spark ignition engine (스파크 점화기관의 열유속 및 열전달 계수에 대한 실험적 연구)

  • Han, Seong-Bin;Gwon, Yeong-Jik;Lee, Jeong-Yeol
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.21 no.11
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    • pp.1466-1474
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    • 1997
  • In order to design and develop a spark ignition engine, many studies must be preceded about the characteristics of thermal flow. For measurement of transient wall temperature thin film thermocouples of Bendersky type were manufactured and these probes were fixed into the wall of combustion chamber. Surface wall temperatures were measured in experiments of various engine speeds. Transient heat fluxes were calculated from the wall temperature measurements. Pressure was measured from combustion chamber using pressure transducer and gas temperatures were calculated using the state equation of ideal gas. And instantaneous heat transfer coefficients were obtained. It will be the basic data for the formulae of instantaneous heat transfer coefficients.

Removal of Endotoxins and Nucleic Acids Using Submicron-sized Polymeric Particles

  • Kim, Chan Wha;Chokyun Rha
    • Journal of Microbiology and Biotechnology
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    • v.6 no.3
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    • pp.189-193
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    • 1996
  • Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently willi the cell debris removal process using SSPP.

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Diffusion-Influenced Kinetics of Reactions Involving Polymers

  • Sung, Jae-Young;Park, Pyeong-Jun;Lee, Jin-Uk;Lee, Woo-Jin;Kim, Ji-Hyun;Lee, Sang-Youb
    • Bulletin of the Korean Chemical Society
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    • v.24 no.6
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    • pp.843-852
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    • 2003
  • We present a brief account of the theory of diffusion-influenced kinetics of reactions involving polymers. The review will be based on the recent contributions from the authors. Both intrapolymer and interpolymer reactions are considered, and the effects of various physical factors, such as the chain length, chain stiffness, and hydrodynamic interactions, are described within a unified theoretical framework.