• 대한의생명과학회지
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• 제12권4호
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• pp.337-341
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• 2006

Platelets clearly play an important role in inflammatory responses. In this study, we aimed to evaluate the relationship between aging and platelet activation. A total number of 799 persons (383 males and 416 females), who were apparently healthy and aged more than 20 years were recruited by a health promotion center in a community-based hospital in Seoul, Korea. We collected material data about their medical history and health behavior. Platelet parameters including mean platelet component (MPC), mean platelet volume (MPV), and platelet component distribution width (PCDW) were determined within 1 hour after blood collection using the ADVIA 120 automated hematology analyzer. The MPC of the women ($27.2{\pm}1.2$) was significantly love. than that of the men ($27.5{\pm}1.3$). The MPC of all participants was found to decrease with increasing age (P<0.01). Study participants in their twenties had the highest MPC ($27.7{\pm}1.1$), followed by those in their thirties ($27.6{\pm}1.1$), forties ($27.4{\pm}1.3$), fifties ($27.2{\pm}1.3$), sixties ($27.2{\pm}1.2$) and seventies ($27.1{\pm}1.2$). Multiple regression analysis showed that aging and gender were related with MPC after adjusting for confounding factors, including age, gender, smoking habit, hypertension, diabetes, body mass index and total cholesterol level. The this study shows that aging is related to platelet activation. Future research will need to determine the implications of increased platelet activation with aging, especially regarding the increased incidence of cardiovascular diseases and related mortalities that occur in older age groups.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.915-921
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• 2013

Background: Hepatocellular carcinoma is one of the leading causes of mortalities worldwide. The search for new therapeutic targets is of utmost importance for improved treatment. Altered expression of HDAC1 in hepatocellular carcinoma (HCC) and its requirement for liver formation in zebrafish, suggest that it may regulate key events in liver carcinogenesis and organogenesis. However, molecular mechanisms of HDAC1 action in liver carcinogenesis are largely unknown. The present study was conducted to identify HDAC1 interacting proteins in HepG2 cells using modified SH-double-affinity purification coupled with liquid mass spectrophotemetery. Materials and Methods: HepG2 cells were transfected with a construct containing HDAC1 with a C-terminal strepIII-HA tag as bait. Bait proteins were confirmed to be expressed in HepG2 cells by western blotting and purified by double affinity columns and protein complexes for analysis on a Thermo LTQ Orbitrap XL using a C18 nano flow ESI liquid chromatography system. Results: There were 27 proteins which showed novel interactions with HDAC1 identified only in this study, while 14 were among the established interactors. Various subunits of T complex proteins (TCP1) and prefoldin proteins (PFDN) were identified as interacting partners that showed high affinity with HDAC1 in HepG2 cells. Conclusions: The double affinity purification method adopted in this study was very successful in terms of specificity and reproducibility. The novel HDAC1 complex identified in this study could be better therapeutic target for treatment of hepatocellular carcinoma.

• 한국수산과학회지
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• 제51권1호
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• pp.42-46
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• 2018

Fetal bovine serum (FBS) is essential for cell culture and is used in the determination of infectivity titer and propagation of viruses. To clarify the effects of FBS on the propagation of viral haemorrhagic septicaemia virus (VHSV), which is a causative agent of mass mortalities of olive flounder Paralichthys olivaceus in Korea, VHSV was inoculated into an EPC (epithelioma papulosum cyprinid) cell line supplemented with MEMs (minimal essential medium) with FBS concentrations of 0%, 2%, 5%, and 10% (MEM0, MEM2, MEM5, and MEM10), respectively, and infectivity titers were compared. Cytopathic effects were observed in all experimental groups at 2 days post virus inoculation (dpi) and all cells were detached from cell culture flasks at 7 dpi. Infectivity titers increased to 3 dpi, persisted to 7 dpi, and decreased when cells were detached. The titer of VHSV in EPC cells in MEM0 was the lowest while those in the other experimental groups showed similar levels. In conclusion, 2% (v/v) of FBS was sufficient to propagate VHSV in EPC cells and the withdrawal of VHSV from cell culture flasks should be performed before cell detachment.

• Ocean and Polar Research
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• 제43권4호
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• pp.365-370
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• 2021

Manila clam Ruditapes philippinarum is present at high rates of density in tidal flats in Cheonsu Bay on the west coast of Korea, where clams often exhibit mass mortalities in late summer. We monitored the pathologic condition of clams at Hwangdo tidal flat (HD) to understand the parasitic impacts on clam fitness. Manila clams were fully ripe in July and spawned during August and September, as the histology indicated. The histology revealed that clams in HD tidal flats were heavily infected by the protozoa parasite Perkinsus olseni, as the monthly prevalence ranged from 53% (September) to 93% (August). In addition, Manila clams were co-infected by the metazoan parasite Cercaria tapetis and Parvatrema duboisi with the prevalence of 0-33% and 0-14%, respectively. Massive hemocyte infiltration and subsequent inflammation were commonly observed from the gills of P. olseni infected clams. Clusters of P. olseni trophozoites and heavy hemocyte infiltration were also observed from the female gonad, suggesting that P. olseni interferes with host gonad maturation. The larval trematode occupied almost the entire host gonad, resulting in gonad castration. In addition, Metacercaria of P. duboisi were observed from the subsurface of the mantle. Ray's fluid thioglycollate medium assay (RFTM) indicated that clams collected in August and September contained approximately 4.0×106 P. olseni cells/g gills. Condition Index (CI) declined gradually from spring to early summer, and the decline in CI was interpreted as a consequence of the heavy parasitism, as the parasites drain the host's net energy to be used in somatic growth and gamete production.

• 한국어병학회지
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• 제34권1호
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• pp.63-70
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• 2021

As a part of research to elucidate the pathogenesis of so called Soft Tunic Syndrome(STS), that caused mass mortalities in the cultured sea squirts, Halocynthia roretzi, the epidemiological and pathological analysis were done to both clinically normal and diseased groups of the farms of Tongyoung and Geoje coastal areas in southeast sea from February to July, 2008. In the histological finding of the tunic, most of individuals showed tunic softness syndromes that included the disarrangement and destruction of tunic fiber with the simultaneous presence of flagellates-like cells, recently suspected as main agents of tunic softness syndromes. Simultaneously, the intensive degenerative changes of the skeletal muscle of diseased sea squirts were recognized. The changes were characterized with the hyalinization and condensation of muscle fibril and hemocytic infiltration in the muscle fibers. Those were thought to be a kind of typical Zenker's necrosis as in the skeletal muscle of higher vertebrates. Besides of the diseased sea squirts, Zenker's necrosis of skeletal muscles were seen in the normal ones. Epidemiological inquiry for diseased groups revealed that the higher incidences of tunic softness syndrome were recorded in the fast growing groups and in the sites presuming the organic pollution. And Higher malondialadehyde(MDA) and glutathione peroxidase(GPx) activity were detected in the groups showing STS. Those results suggested that Zenker's necrosis of body muscles was a kind of"nutritional myopathy" by oxidative stress. Conclusively, it was considered that Zenker's necrosis of body muscles gives an important clue for elucidating pathogenesis of STS of cultured squirts. And it seems that the necrosis were caused by the oxidative stress to body muscle during abnormal rapid growth of sea squirts.

• 한국패류학회지
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• 제29권2호
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• pp.147-154
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• 2013

In Incheon bay, mass mortalities of Manila clam associated with winter storms have been reported. In the present study we have monitored pathologic condition of the clams stranded on the tidal flats by the winter storms occurred in late March to early April in 2007. The field surveyed indicated that mortality of the Manila clam in the study areas ranged 10-15%. Condition index, a ratio of tissue weight to the shell weight, of the stranded clams was significantly lower than the non-stranded normal clams collected from the same locations (p < 0.05), indicating that the stranded clams were comparatively in poor physiological condition. Perkinsus olseni, the protozoan parasite was observed most of clams used in the analysis and the infection prevalence ranged 77-90%. The infection intensity of P. olseni determined using Ray's fluid thioglycollate medium (RFTM) cultivation and the 2M NaOH digestion assay indicated that the clams collected during late March and early April in 2007 involved 67,182-1,124,727 P. olseni cells/g tissue. The infection intensity of clams from Gung-Pyeung was significantly higher than the intensities observed from Dae-Bu and Young-Heung (p < 0.05). No clear correlation was found between the infection intensities of P. olseni in the non-stranded normal clams and the stranded clams. The stranded Manila clams were also infected with trematode parasite with the prevalence ranged 5 (Young-Heung) to 12.5% (Dae-Bu). The trematode-infected clams exhibited castrated follicles in the gonad, a typical sign of trematode infection. It was believed that mass mortality of Manila clam observed in this study was associated with the poor physiological condition as indicated by CI, although impacts of the parasite infection cannot be ruled out.

• 한국어병학회지
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• 제13권2호
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• pp.121-127
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• 2000

1998년 8~10월에 남해안 일원 해상 가두리 양식장에서 사육중인 돌돔, Oplegnathus fasciatus이 60%이상 폐사하였다. 병어의 체색은 검어지거나 퇴색되며 아가미는 빈혈증상이 심하고 비장은 특이적으로 비대되어 있었고, 비장, 신장, 심장 및 간장 조직에서 퓰겐양성과 호염기성의 비대세포가 관찰되었다. 병어의 비장조직 마쇄액을 GF세포에 접종배양한 결과, 세포가 구형화 되면서 커지는 세포변성효과가 나타나 감염된 GF 세포로부터 바이러스가 분리되었다. 분리된 바이러스 배양액($10^4TCID_{50}$/0.1 ml)을 건강한 돌돔 치어에 복강주사하면 자연감염어와 유사한 증상을 나타내면서 폐사가 일어났고, 또한 병어의 비장조직을 전자현미경으로 관찰한 결과, 조직내 비대세포의 세포질에서 직경이 120~130 nm이며 외막이 있는 정 20면체의 바이러스 입자가 관찰되었다. 그리고 genebank로부터 얻은 참돔 이리도바이러스(RSIV)의 ATPase gene의 DNA염기서열을 주형으로 제작된 primer를 사용하여 PCR을 수행한 결과, 500 bp의 PCR 생성물이 병어나 인위감염된 GF세포에서 얻어졌는데, 이들 PCR 생성물은 RSIV의 ATPase cDNA gene과 95%의 상동성을 나타내었다. 따라서 양식돌돔에 대량 폐사를 일으킨 원인 바이러스는 참돔 이리도바이러스(RSIV)와 유사한 이리도바이러스로 밝혀졌다.

• 해양환경안전학회지
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• 제18권6호
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• pp.514-519
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• 2012

전라남도 완도 연안 해역은 전복양식이 중요하고 최근 해상가두리 양식이 보편화되어 전복사육과 폐사에 중요한 수온의 변동을 이해할 필요가 있다. 완도해역에 위치한 신지도, 청산도의 2005년부터 2009년까지 연속자동관측 수온자료를 이용하여 완도 양식장 해역의 수온 변동 특성을 분석하였다. 연안에 가까운 신지도의 연간 수온변동 범위와 수온증가율이 청산도에 비해 크게 나타났으며, 수심이 낮은 연안 해역에서 하계 태양복사열에 의한 가열과 동계의 냉각에 기인한 것으로 판단된다. 또한 추계의 수온 감소율이 춘계의 상승률보다 컸고, 하계에 조석 주기의 단주기 수온변동이 뚜렷하게 나타났다. 급격한 수온변화에 해당하는 큰 수온일교차는 청산도에서 크게 나타났다. 수온 자료로 판단한 전복양식장은 외해에 위치한 청산도가 연안보다 더 적절한 해역으로 판단된다.

• 한국어병학회지
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• 제24권3호
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• pp.197-204
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• 2011

멍게 물렁증 원인규명의 일환으로 2011년 3월에 경남 통영에서 채집된 물렁증 멍게와 건강한 멍게를 대상으로 병리학적 검사를 실시한 결과 물렁증 멍게의 경우 체액량, 비만도 및 피막지수가 현저히 감소하였다. 물렁증 멍게의 피막 imprint 관찰과 배양 시 2개의 편모를 가진 원생동물이 확인되었으며, 물렁증 멍게에 대한 이들 편모충의 감염률은 97.5%인 반면 건강한 멍게에서는 검출되지 않아 본 편모충은 물렁증 멍게에서 특이적으로 검출되는 것으로 조사되었다. 조직학적 검사결과 물렁증 멍게에서 피막섬 유질다발의 붕괴 현상이 관찰되었으며, 편모충으로 추정되는 원생동물들이 다수 관찰되었다. 통영산 물렁증 멍게에서 확인된 편모충은 일본에서 검출된 그것과 매우 유사한 형태적 특징을 갖고 있으며, 향후 공격실험 등 물렁증 유발과 편모충과의 연관성에 대한 추가 연구가 요망된다.

• Journal of Preventive Medicine and Public Health
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• 제43권2호
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• pp.151-158
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• 2010

Objectives: The aim of this study was to evaluate the association between cigarette smoking and total mortality, cancer mortality and other disease mortalities in Korean adults. Methods: A total of 14 161 subjects of the Korean Multi-center Cancer Cohort who were over 40 years of age and who were cancer-free at baseline enrollment reported their lifestyle factors, including the smoking status. The median follow-up time was 6.6 years. During the follow-up period from 1993 to 2005, we identified 1159 cases of mortality, including 260 cancer mortality cases with a total of 91 987 person-years, by the national death certificate. Cox proportional hazard regression model was used to estimate the hazard ratio (HR) of cigarette smoking for total mortality, cancer mortality and disease-specific mortality, as adjusted for age, gender, the geographic area and year of enrollment, the alcohol consumption status, the education level and the body mass index (BMI). Results: Cigarette smoking was significantly associated with an increased risk of total mortality, all-cancer mortality and lung cancer mortality (p-trend, < 0.01, <0.01, <0.01, respectively). Compared to non-smoking, current smokers were at a higher risk for mortality [HR (95% CI)=1.3 (1.1 - 1.5) for total mortality; HR (95% CI)=1.6 (1.1 -2.2) for all-cancer mortality; HR (95% CI)=3.9 (1.9-7.7) for lung cancer mortality]. Conclusions: This study's results suggest that cigarette smoking might be associated with total mortality, all-cancer mortality and especially lung cancer mortality among Korean adults.