Browse > Article
http://dx.doi.org/10.7314/APJCP.2013.14.2.915

Identification of Histone Deacetylase 1 Protein Complexes in Liver Cancer Cells  

Farooq, Muhammad (Bioproducts Research Group, Department of Zoology, College of Science, King Saud University)
Hozzein, Wael N. (Bioproducts Research Group, Department of Zoology, College of Science, King Saud University)
Elsayed, Elsayed A. (Bioproducts Research Group, Department of Zoology, College of Science, King Saud University)
Taha, Nael A. (Bioproducts Research Group, Department of Zoology, College of Science, King Saud University)
Wadaan, Mohammad A.M. (Bioproducts Research Group, Department of Zoology, College of Science, King Saud University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.2, 2013 , pp. 915-921 More about this Journal
Abstract
Background: Hepatocellular carcinoma is one of the leading causes of mortalities worldwide. The search for new therapeutic targets is of utmost importance for improved treatment. Altered expression of HDAC1 in hepatocellular carcinoma (HCC) and its requirement for liver formation in zebrafish, suggest that it may regulate key events in liver carcinogenesis and organogenesis. However, molecular mechanisms of HDAC1 action in liver carcinogenesis are largely unknown. The present study was conducted to identify HDAC1 interacting proteins in HepG2 cells using modified SH-double-affinity purification coupled with liquid mass spectrophotemetery. Materials and Methods: HepG2 cells were transfected with a construct containing HDAC1 with a C-terminal strepIII-HA tag as bait. Bait proteins were confirmed to be expressed in HepG2 cells by western blotting and purified by double affinity columns and protein complexes for analysis on a Thermo LTQ Orbitrap XL using a C18 nano flow ESI liquid chromatography system. Results: There were 27 proteins which showed novel interactions with HDAC1 identified only in this study, while 14 were among the established interactors. Various subunits of T complex proteins (TCP1) and prefoldin proteins (PFDN) were identified as interacting partners that showed high affinity with HDAC1 in HepG2 cells. Conclusions: The double affinity purification method adopted in this study was very successful in terms of specificity and reproducibility. The novel HDAC1 complex identified in this study could be better therapeutic target for treatment of hepatocellular carcinoma.
Keywords
Histone deacetylase 1 (HDAC1); double affinity tag purification; hepatocellular carcinoma;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kocher T, Superti-Furga G (2007). Mass spectrometry-based functional proteomics: from molecular machines to protein networks. Nat Methods, 4, 807-15.   DOI   ScienceOn
2 Lachenmayer A, Toffanin S, Cabellos L, et al (2012). Combination therapy for hepatocellular carcinoma: additive preclinical efficacy of the HDAC inhibitor panobinostat with sorafenib. J Hepatol, 56, 1343-50.   DOI   ScienceOn
3 Lamesch P, Li N, Milstein S, et al (2007). hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics, 89, 307-15.   DOI   ScienceOn
4 Lin HY, Chen CS, Lin SP, Weng JR (2006). Targeting histone deacetylase in cancer therapy. Med Res Rev, 26, 397-413.   DOI   ScienceOn
5 Mori K, Maeda Y, Kitaura H, et al (1998). MM-1, a novel c-Myc-associating protein that represses transcriptional activity of c-Myc. J Biol Chem, 273, 29794-800.   DOI
6 Myung JK, Afjehi-Sadat L, Felizardo-Cabatic M, Slavc I, Lubec G (2004). Expressional patterns of chaperones in ten human tumor cell lines. Proteome Sci, 2, 8.   DOI
7 Nakagawa M, Oda Y, Eguchi T, et al (2007). Expression profile of class I histone deacetylases in human cancer tissues. Oncol Rep, 18, 769-74.
8 Noel ES, Casal-Sueiro A, Busch-Nentwich E, et al (2008).Organ-specific requirements for Hdac1 in liver and pancreas formation. Dev Biol, 322, 237-50.   DOI   ScienceOn
9 Olsen JV, Schwartz JC, Griep-Raming J, et al (2009). A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed. Mol Cell Proteomics, 8, 2759-69.   DOI   ScienceOn
10 Pathil A, Armeanu S, Venturelli S, et al (2006). HDAC inhibitor treatment of hepatoma cells induces both TRAIL-independent apoptosis and restoration of sensitivity to TRAIL. Hepatology, 43, 425-34.   DOI   ScienceOn
11 Qian-Lin Z, Ting-Feng W, Qi-Feng C, Min-Hua Z, Ai-Guo L (2010). Inhibition of cytosolic chaperonin CCTzeta-1 expression depletes proliferation of colorectal carcinoma in vitro. J Surg Oncol, 102, 419-23.   DOI   ScienceOn
12 Quint K, Agaimy A, Di Fazio P, et al (2011). Clinical significance of histone deacetylases 1, 2, 3, and 7: HDAC2 is an independent predictor of survival in HCC. Virchows Arch, 459, 129-39.   DOI   ScienceOn
13 Satou A, Taira T, Iguchi-Ariga SM, Ariga H (2001). A novel transrepression pathway of c-Myc. Recruitment of a transcriptional corepressor complex to c-Myc by MM-1, a c-Myc-binding protein. J Biol Chem, 276, 46562-7.   DOI   ScienceOn
14 Tsuchiya H, Iseda T, Hino O (1996). Identification of a novel protein (VBP-1) binding to the von Hippel-Lindau (VHL) tumor suppressor gene product. Cancer Res, 56, 2881-5.
15 Uetz P, Giot L, Cagney G, et al (2000). A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature, 403, 623-7.   DOI   ScienceOn
16 Vainberg IE, Lewis SA, Rommelaere H, et al (1998). Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell, 93, 863-73.   DOI   ScienceOn
17 Venturelli S, Armeanu S, Pathil A, et al (2007). Epigenetic combination therapy as a tumor-selective treatment approach for hepatocellular carcinoma. Cancer, 109, 2132-41.   DOI   ScienceOn
18 Walhout AJ, Sordella R, Lu X, et al (2000). Protein interaction mapping in C. elegans using proteins involved in vulval development. Science, 287, 116-22.   DOI   ScienceOn
19 Wen YD, Perissi V, Staszewski LM, et al (2000). The histone deacetylase-3 complex contains nuclear receptor corepressors. Proc Natl Acad Sci USA, 97, 7202-7.   DOI
20 Wilson AJ, Byun DS, Popova N, et al (2006). Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J Biol Chem, 281, 13548-58.   DOI   ScienceOn
21 Witt O, Deubzer HE, Milde T, Oehme I (2009). HDAC family: What are the cancer relevant targets? Cancer Lett, 277, 8-21.   DOI   ScienceOn
22 Wong CW, Privalsky ML (1998). Transcriptional repression by the SMRT-mSin3 corepressor: multiple interactions, multiple mechanisms, and a potential role for TFIIB. Mol Cell Biol, 18, 5500-10.
23 Wu LM, Yang Z, Zhou L, et al (2010). Identification of histone deacetylase 3 as a biomarker for tumor recurrence following liver transplantation in HBV-associated hepatocellular carcinoma. PLoS One, 5, 14460.   DOI   ScienceOn
24 Yang Z, Zhou L, Wu LM, et al (2010). Combination of polymorphisms within the HDAC1 and HDAC3 gene predict tumor recurrence in hepatocellular carcinoma patients that have undergone transplant therapy. Clin Chem Lab Med, 48, 1785-91.
25 Yokota S, Yamamoto Y, Shimizu K, et al (2001). Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma. Cell Stress Chaperones, 6, 345-50.   DOI
26 Yue F, Wang LS, Xia L, et al (2009). Modulated T-complex protein 1 zeta and peptidyl-prolyl cis-trans isomerase B are two novel indicators for evaluating lymph node metastasis in colorectal cancer: Evidence from proteomics and bioinformatics. Proteomics Clin Appl, 3, 1225-35.   DOI   ScienceOn
27 Zhang Z, Yamashita H, Toyama T, et al (2005). Quantitation of HDAC1 mRNA expression in invasive carcinoma of the breast*. Breast Cancer Res Treat, 94, 11-6.   DOI   ScienceOn
28 Delcuve GP, Khan DH, Davie JR (2013). Targeting class I histone deacetylases in cancer therapy. Expert Opin Ther Targets, 17, 29-41.   DOI   ScienceOn
29 Brinke A, Green PM, Giannelli F (1997). Characterization of the gene (VBP1) and transcript for the von Hippel-Lindau binding protein and isolation of the highly conserved murine homologue. Genomics, 45, 105-12.   DOI   ScienceOn
30 Coradini D, Speranza A (2005). Histone deacetylase inhibitors for treatment of hepatocellular carcinoma. Acta Pharmacol Sin, 26, 1025-33.   DOI   ScienceOn
31 Farooq M, Sulochana KN, Pan X, et al (2008). Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish. Dev Biol, 317, 336-53.   DOI   ScienceOn
32 Fujioka Y, Taira T, Maeda Y, et al (2001). MM-1, a c-Myc-binding protein, is a candidate for a tumor suppressor in leukemia/lymphoma and tongue cancer. J Biol Chem, 276, 45137-44.   DOI   ScienceOn
33 Guenther MG, Barak O, Lazar MA (2001). The SMRT and N-CoR corepressors are activating cofactors for histone deacetylase 3. Mol Cell Biol, 21, 6091-101.   DOI   ScienceOn
34 Gahr S, Peter G, Wissniowski TT, et al (2008). The histone-deacetylase inhibitor MS-275 and the CDK-inhibitor CYC-202 promote anti-tumor effects in hepatoma cell lines. Oncol Rep, 20, 1249-56.
35 Gingras AC, Gstaiger M, Raught B, Aebersold R (2007). Analysis of protein complexes using mass spectrometry. Nat Rev Mol Cell Biol, 8, 645-54.   DOI   ScienceOn
36 Glatter T, Wepf A, Aebersold R, Gstaiger M (2009). An integrated workflow for charting the human interaction proteome: insights into the PP2A system. Mol Syst Biol, 5, 237.
37 Guenther MG, Yu J, Kao GD, Yen TJ, Lazar MA (2002). Assembly of the SMRT-histone deacetylase 3 repression complex requires the TCP-1 ring complex. Genes Dev, 16, 3130-5.   DOI   ScienceOn
38 Halkidou K, Gaughan L, Cook S, et al (2004). Upregulation and nuclear recruitment of HDAC1 in hormone refractory prostate cancer. Prostate, 59, 177-89.   DOI   ScienceOn
39 Ito T, Chiba T, Ozawa R, et al (2001). A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci USA, 98, 4569-74.   DOI   ScienceOn
40 Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.   DOI
41 Junttila MR, Saarinen S, Schmidt T, Kast J, Westermarck J (2005). Single-step Strep-tag purification for the isolation and identification of protein complexes from mammalian cells. Proteomics, 5, 1199-203.   DOI   ScienceOn
42 Karagianni P, Wong J (2007). HDAC3: taking the SMRT-N-CoRrect road to repression. Oncogene, 26, 5439-49.   DOI   ScienceOn