• 한국학교ㆍ지역보건교육학회지
• /
• 제3권
• /
• pp.59-78
• /
• 2002

The purpose of this study was based on students in high school to find out how interest eating and controlling weight on normal time by sex to let them get a better sense's of view on weight controlling so they can get healthy, and healthy school life and concentrate on studying, As a result of analyzing the data collected for the subject of high school students, the conclusions were as follows; First, out of 325 students, 44.6% were male students, and 179 were female students, Compared to the BMI, normal mass were 50.8%, which were 165 students, less weighted were 39.7%, which were 129 students, and over weighted were 9.5%, which were 31 student. Also average height for male students were 173.8 cm, for female students were 161.5 cm, average weight were 67.2 kg for male students, and for female students were 53.2kg. Using BMI analysing the results and the male students had an average of 22.2%, and the female students had an average of 20.3%, so male students were a little higher than the female students. Second, the interest rate for weight control were 82.2%, that's 267 students for, 'interested' and 7.8%, which were 58 student for 'not interested', so most students were interested, in controlling weight. Interest rate were 83.6% for male students, 81.0% were female students. BMI told that group of less weighted were 82.9%, group of normal were 79.4% and group of over weighted were 93.5%. The rate were all high not relating to BMI. Third, compared to the past, more people said 'normal'(41.3%), people who said 'a little fat'(36.3%) decreased, but they still think they are fat even though they are not, also when they are less weighted they still think they are fat. Fourth, for 'weight control, and food' both male and female said they were related, and for 'weight control and exercising' they also said they were related, but more male said that they were related, However for relations between' controlling weight and school's physical education class' the answers were usually disagree. Fifth, for the 'satisfaction of their present weight', both BMI and the students answered and this results were mostly same as the past result, so most students prefer to lose weight. Also, both male and female think that the reason they have this weight now is, because of 'the amount of exercises' and 'the amount of food they eat', so they find that it's related to each other. Sixth, for the experience on weight control, both male and female had experiences, and they answered 'exercising and food treatment' is the good way to control weight. Also for 'the reason they started to control there weight', both male and female answered, 'they thought there weight were not normal'. Seventh, 'Do you pick on food to control weight?' and 'Do you feel nervous before you eat?' and for last 'control of drinking water' the answer was all different, and both male and female answered negatively. Eighth, time wasted on exercising per day, for less than 30 minute were 81.5%, the form of exercises that students did were 'not much or walking on the way to school and way to home'. Usually for their free time, male students spend on exercising however female student did not. Also both female and male students showed that they like to exercise, but majority of female student disliked to exercise. To everyone's point of view 'like'(32.9%), 'like a lot'(20.9%), so everyone agrees. The knowledge information on controlling weight, they answered, usually found from commercials, newspaper or magazines and from parents or friend. From the past many high school students wanted to control their weight, so there should had been a good education on this, however there wasn't any of those education and still it doesn't exist. Also most school's education are arranged to entrance pressure so they have a lot of knowledge and informations to it, but for real they don't have any activity or actions on it. Through this research, we felt that, we should correct students with wrong understanding on controlling weight and wrong knowledge. Also we suggest to make an activity program for this.

• Journal of Nutrition and Health
• /
• 제50권6호
• /
• pp.552-564
• /
• 2017

본 연구는 20 ~ 60대의 성인 109명 (남 54명, 여 55명)을 대상으로 13가지 대표적 신체활동에 대한 에너지 소비량(EE)및 신체활동 강도 (METs)를 휴대용 호흡가스 분석기($K4b^2$)를 이용하여 측정하고 이를 기준으로 가속도계(Actigraph $GT3X^+$)의 정확성을 성별에 따라 평가하였다. 그 결과를 요약하면 다음과 같다. 1. 휴대용 호흡가스 분석기로 측정한 보통 달리기를 제외한 모든 트레드밀 활동의 단위체중당 에너지 소비량과 METs값은 여자가 남자 보다 유의하게 높았다. 13가지 대표 활동 중 눕기, 앉기 및 서기와 같은 움직임이 없는 활동은 에너지 소비량이 가장 낮은 것으로 나타났고, 남자는 계단 올라가기 (에너지 소비량 포함)에서 여자는 8 km/h 속도의 보통 달리기 (에너지 소비량값 포함)에서 에너지가 많이 소비되는 고강도 활동으로 나타났다. 2. 가속도계 및 휴대용 호흡가스 분석기로 측정한 EE와 METs값을 비교한 결과, 남자는 계단 내려가기, 보통 걷기와 빠르게 걷기 활동에서 여자는 계단 내려가기를 제외한 모든 활동에서, 가속도계가 휴대용 호흡가스 분석기 보다 과소추정 하였다. 3. Compendium 2011 기준에 따라 강도를 분류하였을 때 가속도계와 휴대용 호흡가스 분석기는 저강도 활동에서 일치하는 비율이 가장 높은 (남자 88.3%, 여자 91.3%) 반면, 고강도 활동에서 가장 낮은 일치율을 보였고 여자 (58.3%)보다는 남자 (75.2%)에서 일치율이 유의하게 높았다. 4. 가속도계를 이용하여 EE 및 METs값 추정 시 준거도구와의 차이를 성별에 따라 비교하면, 계단 내려가기를 제외한 모든 활동에서 여자 보다 남자에서 오차가 작은 것으로 나타났다. 특히, 트레드밀 이외의 활동보다는 트레드밀 활동에서의 오차가 작은 것으로 나타났다. 본 연구는 다양한 연령대의 성인 남녀의 신체활동의 에너지 소비량 등을 휴대용 호흡가스 분석기로 측정하였다는데 큰 의의가 있으며 이와 같은 측정값은 한국인을 위한 활동분류표 개발 및 에너지 소비량 산출 시 활용될 수 있을 것이다. 추후 연구에서는 현재까지 개발된 다양한 가속도계 추정식의 정확성이 검증되어야 하며 향후 가속도계를 이용하여 EE 및 METs값 산출 시 필요한 추정식은 적용하는 신체활동 종류뿐만 아니라 성별에 따라 개발되어야 할 것이다.

• 대한환경공학회지
• /
• 제27권3호
• /
• pp.253-261
• /
• 2005

불균일 $CoO_x/TiO_2$ 촉매가 충진된 연속 흐름식 고정층 반응기 내에서 ppm 수준으로 수중에 존재하는 trichloroethylene (TCE) 제거반응을 수행하였으며, 가장 우수한 반응활성을 갖는 촉매의 결정구조와 표면화학적 특성을 분석함으로써 반응시간에 따라 분해활성이 전이영역을 보이는 원인을 규명하고자 하였다. $36^{\circ}C$의 반응온도에서 모델반응의 내부확산저항은 없었으며, $TiO_2$ 표면에 흡착에 의한 액상 TCE 제거정도는 무시할 수 있었다. 5% $CoO_x/TiO_2$ 촉매는 본 대상반응에 대하여 가장 우수한 활성을 갖는 것으로 나타났으며, 반응시간의 경과정도에 따라 TCE 분해효율이 점진적으로 증가하여 안정화되는 전이영역의 존재를 확인할 수 있었다. 반응 전 5% $CoO_x$ 촉매에 대한 XRD 패턴에서 담지체로 사용된 $TiO_2$에 의한 피크들 외에 새로운 피크가 관찰되었고, 5시간 이상 동안 반응한 후에 건조된 촉매의 경우에도 반응 전 촉매의 XRD 피크와 매우 유사하였다. $Co_3O_4$의 XRD 패턴들과 대조한 결과, 5% $CoO_x$ 촉매상에서 Co 화합물에 의해 야기되는 XRD 피크들은 $Co_3O_4$에 의한 것임을 알 수 있었다. 반응물에 노출되지 않은 5% $CoO_x/TiO_2$ 촉매에 대한 XPS 측정은 797.1 eV에서 Co $2p_{1/2}$에 대한 주피크와 함께 781.3 eV에서 Co $2p_{3/2}$에 대한 주피크가 관찰되어졌다. 반응 후 촉매의 경우에는 Co $2p_{3/2}$ 및 Co $2p_{1/2}$의 binding energy들은 각각 780.3과 795.8 eV에서 나타났다. 반응 전 후 촉매상에서 Co $2p_{3/2}$의 binding energy 차이는 1.0 eV이고, Co $2p_{1/2}$의 binding energy 차이는 1.3 eV이다. 표준 $Co_3O_4$에 대한 XPS 측정결과, 반응 후 촉매상에 존재하는 $CoO_x$는 $Co_3O_4$로 존재하고, 반응 전의 경우에는 이와는 다른 chemical state를 보여주었다. XRD 및 XPS 결과를 바탕으로, 촉매표면에 존재하는 $Co_3O_4$의 외부표면이 $Co_2TiO_4$와 $CoTiO_3$ 같은 $CoTiO_x$로 encapsulation되어 있는 모델구조를 제안할 수 있고, 이는 반응시간의 함수로 나타나는 촉매활성에 있어서 전이영역의 존재를 잘 설명할 수 있을 뿐만 아니라, XRD와 XPS에서 얻어진 촉매의 물리화학적인 특성을 잘 반영할 수 있다.

• 근관절건강학회지
• /
• 제1권1호
• /
• pp.1-30
• /
• 1994

Rheumatoid arthritis is a chronic systematic disease with unpredictable course of exacerbation and remission, characterized by pain, joint deformity and reduced activity by joint limitation. The growing public awareness of the need for health management of chronic illnesses, provides impetus for nursing to demonstrate social effectiveness by active nursing intervention in this vast area in general, and with rheumatoid condition in particular. However, nursing interventions to date have not demonstrated its active participation in the management of chronic conditions. Nursing intervention for the patients having rheumatoid arthritis is one such area that needs to be studied intensively and to demonstrate their effectiveness empirically. The purposes of this study were two fold : first, to develop a comprehensive program that was intensified with therapeutic joint exercises in water, self-help group activities and utilization of self-efficacy promoting strategies : and secondly, to determine the effect of aquatic exercise program on pain, physiological changes and quality of life in the rheumatoid arthritis patients. Thirty five female subjects participating in this study were selected from outpatients in the Rheumatism Center, Hanyang University hospital. The period of data collection was from December, 1992 to March, 1993 in seoul and Taejeon. Subjects in Taejeon were assigned to the experimental group. The comprehensive aquatic exercise program for the experimental group was carried out three times a week for 6 weeks in regular swimming pool. Subjects in Seoul did not participated in the program, and treated as the control group. Data were analyzed with repeated measure MANCOVA, t-test, ANCOVA, percentage of change, Kruskal-Wallis 1-Way ANOVA using SPSS $PC^+$ program. Results were obtained as follows : 1) Scores on Korean pain scale, Numeric pain score, and RAI score of the experimental group were significantly lower than those of the control group (t=2.11, p=0.022 ; F=4.40, p=0.044 : t=3.10, p=0.002). 2) There was significant improvement in the physiological parameters (higher joint movement parameters, F=15.64, p=0.024 ; higher lean body mass, percentage of change=+12.2, lower body weight, t=1.01, p=0.026 : lower ESR, t=1.69, p=0.001) in the experimental group compaired with subjects In the control group. 3) There was significant improvement in the specific self-efficacy score through the comprehensive aquatic exercise program(t=2.73, p=0.011), but not in the general self- efficacy score(t=0.62, p=0.113). 4) The quality of life failed to show significant improvement in the experimental group as compaired with the control group (F=3.69, p=0.064). 5) In the experimental group, findings from additional analysis showed no significant difference in the specific self-efficacy between those who continued to aquatic exercise after completing 6 week program and those who stopped (X2=0.086, p=0.690). Therefore, adherence to aquatic exercise program for 6 weeks seem to be affected mainly by self-help group activities. An indepth study to delve into articulation of mechanisms affecting the effect of aquatic exercise program be recommended. A further study is necessary to determine the difference in the effect of group and individual aquatic exercise program, to assess factors affecting adherence to exercise for an extended length of time.

• 생명과학회지
• /
• 제20권8호
• /
• pp.1193-1200
• /
• 2010

상수리 잎을 먹고 자라는 야생견사곤충의 일종인 작잠의 저장단백질인 아릴포린 유전자의 cDNA를 클로닝하고 발육경과에 따른 유전자발현의 양상을 조사 검토하였다. 작잠의 아릴포린 유전자의 cDNA는 2,112 bp의 ORF(open reading frame)를 포함하여 2,234 bp임을 밝혔다. 작잠 아릴포린 유전자의 cDNA염기서열로부터 아미노산 서열을 검토한 결과 방향족 아미노산인 페닐알라닌(phenylalanine)과 티로신(tyrosine)의 성분이 높은 아미노산 서열구조를 보였으며 ORF로부터 계산한 단백질의 분자량은 83,439 Da 이었다.작잠 아릴포린 저장단백질의 아미노산서열을 다른 곤충의 서열과 그 상동성을 비교 분석한 결과 세크로피아잠(H. cecropia)과는 78%, 담배나방의 알파단량체(M. sexta-$\alpha$ subunit)와는 71%, 담배나방의 베타단량체(M. sexta $\beta$-subunit)와는 62% 그리고 가잠(B. mori)과는 64%의 아미노산서열 상동성을 각각 보였다. 또, Northern blot analysis에서 유충 5령기의 중장, 중부 실샘, 후부 실샘에서는 아릴포린 유전자가 발현되지 않고 오로지 지방체 조직에서만 아릴포린 유전자가 발현되었음을 확인하였다. 아릴포린 유전자는 특히 종령인 5령 유충의 초기에 발현강도가 높았으며 중, 후기로 갈수록 그 강도가 감소하였다. 하지만 번데기시기에는 흔적 정도의 해당 mRNA가 검출되었으며, 이와 같이 검출된 mRNA는 불활성화된 것으로 추정된다. 이상의 결과에서 작잠 아릴포린 유전자의 cDNA가 클로닝되었으며, 이 유전자는 유충기특이적 발현 및 지방체 조직특이적 발현양상을 보인 점이 본 연구에서 확인되었다.

• 근관절건강학회지
• /
• 제8권1호
• /
• pp.122-140
• /
• 2001

The aims of this study were to understand and to predict the determinent factors affecting the exercise behaviors and physical fitness by testing the Pender's revised health promotion model, and to help the patients with rheumatoid arthritis and osteoarthritis perform the continous exercise program, and to help them maximize the physical effect such as muscle strength, endurance, and functional status and mental effects including self efficacy and quality of life, and improve the physical and mental well being, and to provide a basis for the nursing intervention strategies. Of the selected variables in this study, the endogenous variables included the physical fitness, exercise score, exercise participation, perceived benefits of action, perceived barriers of action to exercise, activity-related affect(depression) and perceived self-efficacy, interpersonal influences(family support), situational factors(duration of arthritis, fatigue) and the exogenous variables included personal sociocultural factor(education level), personal biologic factor(body mass index), personal psychologic factor(perceived health status) and prior related behavior factors(previous participation in exercise, life-style). We analyzed the clinical records of 208 patients with rheumatoid arthritis and degenerative arthritis who visited the outpatient clinics at H university hospital in Seoul. Data were composed of self reported qustionnaire and good of fitness score which were obtained by padalling the ergometer of bicycle for 9 minutes. SPSS Win 8.0 and Window LISREL 8.12a were used for statistical analysis. Of 75 hypothetical paths that influence on physical fitness, exercise participation, exercise score, perceived benefits of action, perceived barriers of action to exercise, activity-related affect(depression) and perceived self-efficacy, interpersonal influences(family support), situational factors(duration of arthritis, fatigue), 40 were supported. The physical fitness was directly influenced by life-style, perceived health status, education level, family support, fatigue, which explained 12% of physical fitness. The exercise participation were directly influenced by life-style, education level, past exercise behavior, perceived benefits of action, perceived barriers of action, depression and duration of arthritis, which explained 47% of exercise participation. Exercise score were directly affected by perceived self efficacy. BMI, life-style, past exercise behavior, perceived benefits of action, family support, perceived health status. perceived barriers of action, and fatigue, which explained 70%. Perceived benefits of action was directly influenced by BMI, life-style, which explained 39%. Perceived barriers of action were directly influeced by past exercise behavior, perceived health status, which explained 7%. Perceived self efficacy were directly influeced by level of education, perceived health status, life-style, which explained 57%. Depression were directly influeced by past exercise behavior, BMI, life-style, which explained 27%. Family support were directly influeced by life-style, perceived health status, which explained 29%. Fatigue were directly influeced by BMI, life-style, perceived health status. which explained 41%. Duration of arthritis were directly influeced by life-style, past exercise behavior, BMI, which explained 6%. In conclusion, important variables for physical fitness were life-style, and variable affecting exercise participation were life-style. Perceived self-efficacy of exercise was a significant predictor of exercise score. BMI, Life-style, perceived benefits of action, family support, past exercise behavior showed direct effects on perceived self-efficacy. Therefore, disease related factor should be minimized for physical performance and well being in nursing intervention for patients with rheumatoid arthritis, and plans to promote and continue exercise should be seeked to reduce disability. In addition, Exercise program should be planned and performed by the exact evaluation of exercise according to the ability of the patients and the contents to improve the importance of exercise and self efficacy in self control program, dedicated educational program should be involved. This study suggest that the methods to reduce the disease related factors, the importance of daily life-style, recognition of benefit of exercise, and educational program to promote self efficacy should be considered in the exercise behavior promotion and nursing intervention for continous performance. The significance of this study is also thought to provide patients with chronic arthritis the specific data for maximal physical and mental well being through exercise, chronic therapeutic procedure, daily adaptation and confrontation in nursing intervention.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.194-194
• /
• 2017

Camelina (Camelina sativa L.) is a potential bio-energy crop that has short life cycle about 90 days and contains high amount of unsaturated fatty acid which is adequate to bio-diesel production. Enhancing environmental stress tolerance is a main issue to increase not only crop productivity but also big mass production. CsRCI2s (Rare Cold Inducible 2) are cold and salt stress related protein that localized at plasma membrane (PM) and assume to be membrane potential regulation factor. These proteins can be divide into C-terminal tail (CsRCI2D/E/F/G) or no-tail group (CsRCI2A/B/C/H). However, function of CsRCI2s are less understood. In this study, physiological responses and functional characterization of CsRCI2s of Camelina under salt stress were analyzed. Full-length CsRCI2s (A/B/E/F) and CsPIP2;1 sequences were confirmed from Camelina genome browser. Physiological investigations were carried out using one- or four-week-old Camelina under NaCl stress with dose and time dependent manner. Transcriptional changes of CsRCI2A/B/E/F and CsPIP2;1 were determined using qRT-PCR in one-week-old Camelina seedlings treated with NaCl. Translational changes of CsRCI2E and CsPIP2;1 were confirmed with western-blot using the antibodies. Water transport activity and membrane potential measurement were observed by cRNA injected Xenopus laevis oocyte. As results, root growth rate and physiological parameters such as stomatal conductance, chlorophyll fluorescence, and electrolyte leakage showed significant inhibition in 100 and 150 mM NaCl. Transcriptional level of CsPIP2;1 did not changed but CsRCI2s were significantly increased by NaCl concentration, however, no-tail type CsRCI2A and CsRCI2B increased earlier than tail type CsRCI2E and CsRCI2F. Translational changes of CsPIP2;1 was constitutively maintained under NaCl stress. But, accumulation of CsRCI2E significantly increased by NaCl stress. CsPIP2;1 and CsRCI2A/B/E/F co-expressed Xenopus laevis oocyte showed decreased water transport activity as 61.84, 60.30, 62.91 and 76.51 % at CsRCI2A, CsRCI2B, CsRCI2E and CsRCI2F co-expression when compare with single expression of CsPIP2;1, respectively. Moreover, oocyte membrane potential was significantly hyperpolarized by co-expression of CsRCI2s. However, higher hyperpolarized level was observed in tail-type CsRCI2E and CsRCI2F than others, especially, CsRCI2E showed highest level. It means transport of $Na^+$ ion into cell is negatively regulated by expression of CsRCI2s, and, function of C-terminal tail is might be related with $Na^+$ ion influx. In conclusion, accumulation of NaCl-induced CsRCI2 proteins are related with $Na^+$ ion exclusion and prevent water loss by CsPIP2;1 under NaCl stress.

• 생명과학회지
• /
• 제30권4호
• /
• pp.343-351
• /
• 2020

마가목은 한방에서 기침, 천식, 기관지 질환 등의 치료에 이용되고 있다. 본 연구에서는 마가목(sorbus commixta) 수피의 chloroform 분획물로부터 3종의 triterpenoid 화합물과 1종의 coumarin 화합물을 분리하였다. 분리된 화합물의 구조는 MS와 1D-, 2D-NMR분석에 의해 확인하였으며, 이들은 lupeol, β-sitosterol, ursolic acid와 scopoletin으로 구조동정되었다. 분리된 화합물 중 scopoletin은 마가목에서 처음으로 분리된 화합물이다. Scopoletin은 식물에 널리 분포하고 있는 물질로써 항염증 활성을 가진 기능성 화합물이다. 분리된 화합물들의 혈관 염증 억제에 대한 효과를 평가하기 위해 in vitro에서 LDL 산화 억제능을 평가한 결과, 분리된 화합물 중 scopoletin (IC₅₀=10.2 μM)이 강한 억제 활성을 나타내었다. TNF-α로 활성화된 인체혈관내피세포(EA.hy926)를 이용한 실험에서 scopoletin은 세포부착인자인 ICAM-1, VCAM-1, E-selectin의 발현을 저해하였고, THP-1 단핵구와 EA.hy926 혈관내피세포 간의 부착력도 약화시켰다. 뿐만 아니라, scopoletin은 TNF-α로 유도된 NF-κB 전사인자의 핵내 이동 및 IκBα의 인산화도 저해하였다. 따라서 마가목 추출물로부터 분리된 scopoletin은 NF-κB 신호전달의 억제를 통해 세포부착인자의 발현을 감소시키고, 단핵구의 혈관내피세포로의 부착을 억제시켜 혈관내 항염증 활성을 나타내었다. 이러한 실험결과, scopoletin은 혈관염증 반응으로부터 유도되는 죽상동맥경화증 치료를 위한 후보소재로서 이용될 가능성이 있다고 사료된다.

• 생명과학회지
• /
• 제24권3호
• /
• pp.242-251
• /
• 2014

국내에서 자생하는 P. thunbergiana 잎의 메탄올 추출물로부터 사람의 간암세포주, SK-HEP-1에 apoptosis를 유도하는 광과민성물질을 찾기 위해 연구를 시작하였다. Apoptosis를 유도하는 광과민성물질을 순수분리하기 위해 column chromatography법과 TLC법을 이용하였으며, 순수분리한 물질의 구조동정을 위하여 1D-NMR과 2D-NMR 및 FAB-MS분석을 실시하였다. P. thunbergiana 잎으로부터 크로마토그래피법을 이용해 순수분리한 M4-3 화합물의 흡광도를 측정한 결과 410 nm에서 최대 흡수를 보였고, 668, 502, 533, 607 nm에서 흡수 peak가 나타나는 녹색의 물질로 chlorophyll a, b와는 다른 물질이었다. FAB-MS 분석결과와 NMR 분석결과, 분자량 622의 132-hydroxy pheophorbide a methyl ester($C_{36}H_{28}N_2O_6$) 화합물로 porphyrin 환으로부터 Mg이온이 떨어져나간 pheophorbide a유도체화합물로 동정되었다. M4-3화합물을 SK-HEP-1세포에 대한 세포독성을 조사한 결과, 0.04 ${\mu}M$ 농도로 처리한 곳에서는 40% 세포증식 억제효과가 나타났고, 0.08 ${\mu}M$ 농도로 처리한 곳에서는 80% 세포증식 억제효과가 나타나 농도의존적이었다. 그리고 M4-3화합물을 동일한 농도로 처리하고, 광의 조사 유무에 따른 활성차이를 조사한 결과, 광을 조사한 곳에서만 세포독성이 나타났기 때문에 M4-3화합물은 광과민성물질이라는 것을 알 수 있었다. M4-3화합물을 처리하고 세포의 형태학적 변화를 관찰한 결과, 핵이 응축되었고, 소낭이 형성되었으며, DNA 단편화가 일어나는 등 apoptosis의 대표적인 현상들이 일어났기 때문에 M4-3화합물의 세포사멸은 광역학활성에 의한 apoptosis유도로 확인하였다.

• 한국식품과학회지
• /
• 제30권3호
• /
• pp.630-637
• /
• 1998

Aspergillus niger 유래의 TG를 공유결합법으로 고정화시키고 고정화된 TG에 대한 효소적 특성을 soluble TG와 비교하였다. Soluble TG의 $K_m$은 21 mM, $V_{max}$는 0.4 mM/min 인데 비해, 고정화 TG의 $K_m$은 184 mM, $V_{max}$는 7.4 mM/min로 나타났다. 고정화 TG는 soluble TG와 같이 최적 pH도 5.0으로 동일하였으나 고정화 TG가 soluble TG에 비해 pH 6.0에서는 45%, pH 7.0에서 16% 더 높은 활성을 보였다. pH에 대한 안정성은 두 형태의 효소 모두 pH 2.0에서 pH 9.0까지의 비교적 넓은 pH 범위에서 90%이상의 활성이 유지되었다. 고정화 TG의 반응최적 온도는 soluble TG가 $60{\sim}70^{\circ}C$인데 반해 $70{\sim}80^{\circ}C$로 향상되었다. 고정화 TG의 열안정성은 soluble TG보다 $32{\sim}40%$이상 더 높은 활성이 유지되었다. 고정화 TG의 열불활성화 곡선으로부터 구한 D-value는 $65^{\circ}C$에서 7690초, $70^{\circ}C$에서 200초, $75^{\circ}C$에서 83초, $80^{\circ}C$에서는 7.2초이고 Z-value는 soluble TG가 $6.4^{\circ}C$, 고정화 TG가 $5.3^{\circ}C$로 나타났다. Soluble TG와 고정화 TG의 효소반응 활성화에너지는 각각 $7.7{\times}10^3\;J/mol,\;8.8{\times}10^3\;J/mol$이었다. 고정화 TG의 반응중 물질전달의 저항은 거의 없는 것으로 판단되었다.