• Mass Spectrometry Letters
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• 제14권4호
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• pp.153-159
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• 2023

The copper ion, Cu(II), binding sites for amyloid fragment Aβ1-16 (=Aβ16 ) were investigated to explain the biological activity difference in the Aβ16 aggregation process. The [M+Cu+(z-2)H]^z+ (z = 2, 3 and 4, M = Aβ16 monomer) and [D+Cu+(z-2)H]^z+ (z = 3 and 5, D = Aβ16 dimer) structures were investigated using electrospray ionization (ESI) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Fragment ions of the [M+Cu+(z-2)H]^z+ and [D+Cu+(z-2)H]^z+ complexes were observed using collision-induced dissociation MS/MS. Three different fragmentation patterns (fragment "a", "b", and "y" ion series) were observed in the MS/MS spectrum of the (Aβ16 monomer or dimer-Cu) complex, with the "b" and "y" ion series regularly observed. The "a" ion series was not observed in the MS/MS spectrum of the [M+Cu+2H]⁴⁺ complex. In the non-covalent bond dissociation process, the [D+Cu+3H]⁵⁺ complex separated into three components ([M+Cu+H]³⁺, M³⁺, and M²⁺), and the [M+Cu]²⁺ subunit was not observed. The {M + fragment ion of [M+Cu+H]³⁺} fragmentation pattern was observed during the covalent bond dissociation of the [D+Cu +3H]⁵⁺ complex. The {M + [M+Cu+H]³⁺} complex geometry was assumed to be stable in the [D+Cu+3H]⁵⁺ complex. The {M + fragment ion of [M+Cu]²⁺} fragmentation pattern was also observed in the MS/MS spectrum of the [D+Cu+H]³⁺ complex. The {M + [y₉+Cu]¹⁺} fragment ion was the characteristic fragment ion. The [D+Cu+H]³⁺ and [D+Cu+3H]⁵⁺ complexes were likely to form a monomer-monomer-Cu (M-M-Cu) structure instead of a monomer-Cu-monomer (M-Cu-M) structure.

• Nuclear Engineering and Technology
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• 제52권5호
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• pp.1036-1042
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• 2020

Fast-Neutron Multiplicity Counter based on Liquid Scintillator Detector can directly measure the fast neutron multiplicity emitted by UO₂ rod. HPGe gamma spectrometer; which has superior energy resolution; is routinely used for the gamma energy spectrum measurement. Combing Fast-Neutron Multiplicity Counter and HPGe γ-spectrometer, the n/γ joint measurement system is developed. The fast neutron multiplicity and gamma energy spectrum of UO₂ rod assemblies under different conditions are measured by the n/γ joint measurement system. The induced fission rate and the ²³⁵U abundance, thereby the ²³⁵U mass; can be obtained for UO₂ rod assemblies. The ²³⁵U mass deviation of the measured value from the reference value is less than 5%. The results show that the n/γ joint measurement system is effective and applicable in the measurement of the ²³⁵U mass in samples.

• Mass Spectrometry Letters
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• 제12권4호
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• pp.179-185
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• 2021

Collision-induced dissociation (CID) combined with electrospray ionization mass spectrometry (ESI-MS) was used to obtain structural information on rat islet amyloid polypeptide (rIAPP) monomers (M) and dimers (D) observed in the multiply charged state in the MS spectrum. MS/MS analysis indicated that the rIAPP monomers adopt distinct structures depending on the molecular ion charge state. Peptide bond dissociation between L₂₇ and P₂₈ was observed in the MS/MS spectra of rIAPP monomers, regardless of the monomer molecular ion charge state. MS/MS analysis of the dimers indicated that D⁵⁺ comprised M²⁺ and M³⁺ subunits, and that the peptide bond dissociation process between the L₂₇ and P₂₈ residues of the monomer subunit was also maintained. The observation of (M+ b₂₇)⁴⁺ and (M+ y₁₀)³⁺ fragment ions were deduced to originate from the two different D⁵⁺ complex geometries, the N-terminal and C-terminal interaction geometries, respectively. The fragmentation pattern of the [M_rIAPP + M_hIAPP]⁵⁺ MS/MS spectrum showed that the interaction occurred between the two N-terminal regions of M_rIAPP and M_hIAPP in the heterogeneous dimer (hetero-dimer) D⁵⁺ structure.

• 국제초고층학회논문집
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• 제9권3호
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• pp.273-282
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• 2020

An innovative high-rise timber-concrete hybrid structure was proposed in previous research, which is composed of the concrete frame-tube structure and the prefabricated timber modules as main structure and substructures, respectively. Considering that the timber substructures are built on the concrete floors at a different height, the floor response spectrum is more effective in estimating the seismic response of substructures. In this paper, the floor response spectra of the hybrid structure with different structural parameters were calculated using dynamic time-history analysis. Firstly, one simplified model that can well predict the seismic response of the hybrid structure was proposed and validated. Then the construction site, the mass ratio and the frequency ratio of the main-sub structure, and the damping ratio of the substructures were discussed. The results demonstrate that the peaks of the floor response spectra usually occur near the vibration periods of the whole structure, among which the first two peaks stand out; In most cases, the acceleration amplification effect on substructures tends to be more evident when the construction site is farther from the fault rupture; On the other hand, the acceleration response of substructures can be effectively reduced with an appropriate increase in the mass ratio of the main-sub structure and the damping ratio of the substructures; However, the frequency ratio of the main-sub structure has no discernible effect on the floor response spectra. This study investigates the characteristics of the floor response spectrum of the novel timber-concrete structure, which supports the future applications of such hybrid structure in high-rise buildings.

• Bulletin of the Korean Chemical Society
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• 제26권5호
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• pp.763-768
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• 2005

An improved tandem time-of-flight (TOF) mass spectrometer for the photodissociation (PD) study of ions generated by matrix-assisted laser desorption ionization (MALDI), MALDI-TOF-PD-TOF, has been designed and constructed. Recording a full spectrum with better than unit mass resolution even in low mass range has been achieved without reflectron voltage stepping which was needed in the previous version. Other aspects of the improvement, such as those in the data system which now allow 10-100 times faster spectral acquisition than with the previous instrument, are described. Rationale for the ideas which have led to the improvements is presented also.

• 천문학회지
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• 제29권spc1호
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• pp.143-144
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• 1996

Two-component models (normal star and degenerate star components) are the simplest realization of clusters with a mass spectrum because the high mass stars quickly evolve off leaving degenerate stars behind, while low mass stars survive for a long time as main-sequence stars. In the present study we examine the post-collapse evolution of globular clusters using two-component Fokker-Planck models that include three-body binary heating. We confirm that a simple parameter ${\epsilon}{\equiv} (E_{tot}/t_{rh})/(E_c/t_{rc})$ well describes the occurrence of gravothermal oscillations of two-component clusters. Also, we find that the degree of instability depends on the steepness of the mass function such that clusters with a steeper mass function are less exposed to instability.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 추계학술대회논문초록집
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• pp.336.2-336
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• 2002

There are many researches to identify the rigid body properties from the mass-line obtained by impact hammer testing. The correct rigid body properties of the structure may be estimated if the mass-line of the structure could be obtained exactly. When the structure is mounted by elastic materials, the mass-line cannot be read correctly from the impulse response spectrum. The reason is due to the effects of rigid body modes of mounted structure. (omitted)

• Bulletin of the Korean Chemical Society
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• 제18권1호
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• pp.32-37
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• 1997

We have built a laser ionization mass spectrometer (LIMS) for chemical composition analysis of solid samples, which employs an Nd:YAG laser and a time-of-flight mass analyzer. In this spectrometer, the maximum mass we identified clearly is higher than 2000 amu. A mass resolution of 230 has been achieved at m/z 208 (Pb element) in the linear TOFMS and can be even improved up to 1550 by employing a reflectron. The detection limit is determined to be on the order of ppm for Fe and In. The depth resolution is found to be about 20Å/spectrum with a laser power of 0.5 J/cm2. We also report a preliminary application of the LIMS to identifying impurities resident in several solid samples.

• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.3103-3106
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• 2014

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2001년도 가을 학술발표회 논문집
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• pp.561-568
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• 2001

For obtaining the time history nodal responses of reactor building, a lumped-mass beam model composed of two sticks for the reactor building and the reactor support structure is developed. The time history responses for the non-isolated and isolated reactor buildings are calculated under an artificial time history, generated using the seismic spectrum curve of US NRC RG1.60. The analysis results show that the horizontal accelerations of the isolated building are dramatically decreased to one-tenths of the non-isolated one, but the vertical responses are increased by about 40%.