• 유체기계공업학회:학술대회논문집
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• 2003.12a
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• pp.697-703
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• 2003

For a single stage two cylinder rotary compressor, an analytical study has been made on the discharge gas pulsation. Discharge system of the twin rotary compressor consists of lower and upper mufflers and connecting passage holes between them, and cavities on both sides of the motor and passages between them. Acoustic modeling for the discharge system by transfer matrix method gives acoustic impedances at discharge valves so that gas pulsation at the valve sections can be obtained from discharge mass velocity. Since the mass velocity and the pressure pulsation at the valves are affected by each other, iteration should be made for convergence. Gas pulsations at other sections can also be calculated by using transfer matrix.

• Bulletin of the Korean Chemical Society
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• v.17 no.11
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• pp.1036-1039
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• 1996

Synthetic lipoidal peptides based on viral protein sequences have been prepared. These peptides contain an N-palmitoyl group at the N-terminal residue, which is a modified cysteine, containing a S-[2,3-bis(acyloxy)-(2-R,S)-propyl] moiety. When this residue (Pam3Cys) is at the N-terminus of a synthetic peptide, it acts as potent immunoadjuvant to enhance both IgM and IgG antibody responses to the attached peptide. Conventional analytical procedures (e.g., Edman degradation and amino acid analysis) are either not applicable due to the N-terminal modification, or do not provide confirmation of the intact structure. Chromatographic analysis is also hindered by the tendency of these lipoidal Pam3Cys peptides to form large aggregates, and in some cases to be permanently adsorbed on reversed phase columns. We have applied several mass spectrometric techniques, including fast atom bombardment (FAB), electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) to characterize the intact structures of a number of different Pam3Cys synthetic peptides. The MALDI-MS has been found to be the most sensitive for the analysis of the structure of Pam3Cys peptides.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.147.2-147.2
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• 2003

A fast and matrix-assisted laser desorption/ionization-mass spectrometry compatible protein staining method in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis is described. It is based on the counterion dye staining method that employs oppositely charged two dyes, Zincon and Ethyl Violet to form an ion-pair complex. It is safe to use since the methanol used previously in staining solution was replaced with ethanol, which is not toxic. The protocol including fixing, staining and quick washing steps can be completed in 1 to 1.5 h depending upon gel thickness. (omitted)

• Steel and Composite Structures
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• v.46 no.1
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• pp.107-114
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• 2023

A new intelligent adaptive control scheme was proposed that combines observer disturbance-based adaptive control and fuzzy adaptive control for a composite structure with a mass-adjustable damper. The most important advantage is that the control structures do not need to know the uncertainty limits and the interference effect is eliminated. Three adjustable parameters in LMI are used to control the gain of the 2D fuzzy control. Binary performance indices with weighted matrices are constructed to separately evaluate validation and training performance using the revalidation learning function. Determining the appropriate weight matrix balances control and learning efficiency and prevents large gains in control. It is proved that the stability of the control system can be ensured by a linear matrix theory of equality based on Lyapunov's theory. Simulation results show that the multilevel simulation approach combines accuracy with high computational efficiency. The M-TMD system, by slightly reducing critical joint load amplitudes, can significantly improve the overall response of an uncontrolled structure.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.3
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• pp.212-217
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• 2005

Desorption/ionization on silicon mass spectrometry (DIOS-MS) is a relatively new laser desorption/ionization technique for mass spectrometry without employing an organic matrix. This present study was carried to survey the experimental factors to improve the efficiency of DIOS-MS through electrochemical etching condition in structure and morphological properties of the porous silicon. The porous structure of silicon structure and its properties are crucial for the better performance of DIOS-MS and they can be controlled by the suitable selection of electrochemical conditions. The fabrication of porous silicon and ion signals on DIOS-MS were examined as a function of silicon orientation, etching time, etchant, current flux, irradiation, pore size, and pore depth. We have also examined the effect of pre- and post-etching conditions for their effect on DIOS-MS. Finally, we could optimize the electrochemical conditions for the efficient performance of DIOS-MS in the analysis of small molecule such as amino acid, drug and peptides without any unknown noise or fragmentation.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1990-1994
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• 2006

Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited a high GA productivity. A gas chromatography-mass spectrometry (GC-MS) analysis of extracts of the culture fluid of F proliferatum KGL0401 also revealed the presence of $GA_1$, $GA_3$, $GA_4$, $GA_7$, $GA_{20}$, and $GA_{24}$. Therefore, the present study conducted a proteome analysis of waito-c rice treated with the culture fluid of the isolated F proliferatum KGL0401 to identify the protein expression triggered by the GA-containing culture fluid. The results revealed the overexpression of 180 protein spots in the sample treated with the culture fluid. Among them, 75 induced proteins were selected and analyzed by MALDI-TOF (matrix-assisted laser desorption-iorrization time-of-flight) mass spectrometry, followed by database searching, and 51 proteins were identified.

• Structural Engineering and Mechanics
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• v.22 no.6
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• pp.701-717
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• 2006

In the existing reports regarding free transverse vibrations of the Euler-Bernoulli beams, most of them studied a uniform beam carrying various concentrated elements (such as point masses, rotary inertias, linear springs, rotational springs, spring-mass systems, ${\ldots}$, etc.) or a stepped beam with one to three step changes in cross-sections but without any attachments. The purpose of this paper is to utilize the numerical assembly method (NAM) to determine the exact natural frequencies and mode shapes of the multiple-step Euler-Bernoulli beams carrying a number of lumped masses and rotary inertias. First, the coefficient matrices for an intermediate lumped mass (and rotary inertia), left-end support and right-end support of a multiple-step beam are derived. Next, the overall coefficient matrix for the whole vibrating system is obtained using the numerical assembly technique of the conventional finite element method (FEM). Finally, the exact natural frequencies and the associated mode shapes of the vibrating system are determined by equating the determinant of the last overall coefficient matrix to zero and substituting the corresponding values of integration constants into the associated eigenfunctions, respectively. The effects of distribution of lumped masses and rotary inertias on the dynamic characteristics of the multiple-step beam are also studied.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.7
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• pp.562-569
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• 2003

An iterative modal analysis approach is developed to determine the effect of transverse open cracks on the dynamic behavior of simply supported pipe conveying fluid subject to the moving mass. The equation of motion Is derived by using Lagrange’s equation. The influences of the velocity of moving mass and the velocity of fluid flow and a crack have been studied on the dynamic behavior of a simply supported pipe system by numerical method. The presence of crack results In higher deflections of pipe. The crack section is represented by a local flexibility matrix connecting two undamaged beam segments i.e. the crack is modelled as a rotational spring. Totally. as the velocity of fluid flow and the crack severity are increased, the mid-span deflection of simply supported pipe conveying fluid Is Increased. The time which produce the maximum dynamic deflection of the simply supported pipe Is delayed according to the increment of the crack severity.

• Journal of the Korean Society for Precision Engineering
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• v.27 no.2
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• pp.109-116
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• 2010

It is important to make a mechanical structure precisely and reasonably in predicting the dynamic characteristics, controlling the vibration, and designing the structure dynamics. In finite element analysis model updating is appropriate as the design parameter is used to analyze the dynamic system. The errors can be contained from the physical parameters and the element modeling. From the dynamic test, more precise dynamic characteristics can be obtained. In this paper, model updating algorithm is developed using frequency difference between experiment and calculation. Modal frequencies are obtained by experiment and finite element analysis for beams with various cross section and shapes which have added masses and holes in the middle. For plates with and without groove, experiment and analyses are carried out by applying free boundary conditions as well. Mass and stiffness matrices are updated by comparing test and analytical modal frequencies. The result shows that the updated frequencies become closer to the test frequencies in case that both matrices are updated. An improved analytical model is obtained by changing model parameters such that the discrepancy between test and finite element frequencies is minimized. For beam and plate models updating of mass and stiffness matrices can improve the dynamical behavior of the model by acting on the physical parameters such as masses and stiffness.

• Macromolecular Research
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• v.11 no.5
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• pp.341-346
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• 2003

A poly(ethylene oxide)-b-poly(L-lactide) diblock copolymer (PEO-b-PLLA) is characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a block length distribution map is constructed. Although the MALDI- TOF mass spectrum of PEO-b-PLLA is very complicated, most of the polymer species were identified by isolating the overlapped isotope patterns and by fitting the overlapped peaks to the Schulz-Zimm distribution function. Reconstructed MALDI-TOF MS spectrum was nearly identical to the measured spectrum and this method shows its potential to be developed as an easy and fast analysis method of low molecular weight block copolymers.