• The Journal of Information Systems
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• v.23 no.3
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• pp.25-46
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• 2014

As the use of smartphone has rapidly spread into the current society, the mobile instant messenger applications such as Kakaotalk, Tiktok, and Line have become an indispensable part of our daily lives. While, the mobile instant messenger has brought us many positive changes, social problems stemming from the dependency on the mobile instant messenger has been occurred. The dependency of the mobile instant messenger can negatively affect daily lives and lead to stress or addiction. Therefore, it is very important to examine how we can prevent such dependency and addiction. In this respect, the purpose of this study is to investigate determinants impacting on the mobile instant messenger dependency, barrier of living, and intention to digital break. The statistical analysis of survey results shows that enjoyment, identity, relationship commitment, critical mass and ease of use are significantly related to mobile instant messenger dependency, while media richness is not significantly related. Mobile instant messenger dependency have a positive effect on barrier of living, which then has a positive effect on digital break. Lastly, the moderating effect of perceived risk is significant. This research suggests theoretical and business implications to prevent mobile instant messenger dependency.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.872-877
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• 2013

In a previous study, we isolated and reported a second species of the Saccharophagus genus, Saccharophagus sp. strain Myt-1. In the present study, an alginate lyase gene (algMytC) from the genomic DNA of Myt-1 was cloned and characterized. The DNA sequence fragment obtained contained an open reading frame of 1,032 bp that encoded a protein of 343 amino acids with an estimated molecular mass of 37.6 kDa and a pI of 6.60. The deduced protein, AlgMytC, had the conserved amino acid sequences (RTELREM, QIH, YFKAGVYNQ) of the polysaccharide lyase family 7. A BLAST homology search indicated that AlgMytC shared an amino acid sequence identity of 95.9% with alg7A of S. degradans 2-40. The cloned and purified AlgMytC protein showed optimal activity at $40^{\circ}C$, and retained more than 90% of its total activity even after treatment at $25^{\circ}C$ for 24 h. AlgMytC was very alkaliphilic with an optimal pH of 9.0, and more than 90% of its activity was retained in the pH range 8.5-10.0. Moreover, AlgMytC was stable over a wide pH range. The activity of AlgMytC was also stable in the presence of various detergents.

• Journal of Microbiology and Biotechnology
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• v.9 no.3
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• pp.249-254
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• 1999

A catechol 2,3-dioxygenase (C23O) was purified to apparent homogeneity from Pseudomonas putida SU10 through several purification steps consisting of ammonium sulfate precipitation and chromatographies on DEAE 5PW, Superdex S-200, and Resource-Q. Gel filtration indicated a molecular mass under nondenaturing conditions of about 130 kDa. The enzyme has a subunit of 34 kDa as was determined by SDS-PAGE. These results suggest that the native enzyme is composed of four identical subunits. The N-terminal amino acid sequence (30 residues) of the enzyme has been determined and exhibits high identity with other extradiol dioxygenases. The reactivity of this enzyme towards catechol and methyl-substituted catechols is somewhat different from that seen for other catechol 2,3-dioxygenases, with 4-methylcatechol cleaved at a higher rate than catechol or 3-methylcatechol. $K_m$ values of the enzyme for these substrates are between 3.5 and 5.7 M.

• International Journal of Industrial Entomology and Biomaterials
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• v.5 no.1
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• pp.93-102
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• 2002

We have cloned and characterized the complete fibroin light chain gene from the silkworm Baekok-Jam, Bombyx mori, a recommended variety in Korea. It consists of seven exons and six introns. It consists of 14,663 nucleotides long with an open reading frame of 786 nucleotides that encodes a protein of 262 amino acid residues with a molecular mass of approximately 26,000 dalton. The amino acid alignment revealed that the Baekok-Jam fibroin light chain had 98.5 % protein sequence identity to J139 strain: differed at four amino acid positions (11, 46, 80 and 123). The Northern hybridization analysis showed that the Baekok-Jam fibroin light chain gene was specifically expressed in the posterior silk gland.

• Journal of Microbiology
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• v.41 no.2
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• pp.102-108
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• 2003

A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the veetor pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed activesite region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, the could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RBI.

• Journal of the Korean housing association
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• v.17 no.2
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• pp.63-75
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• 2006

The exploration of the spatial structure of a particular urban area, or the analysis of the tendencies of spatial consumption among urbanites, can be a literary-geographical attitude, shifting literary interests toward geography. It may also constitute a field of cultural geography that reads texts as cultural symbols. Based on this kind of attitude, the paper reads the literature of Park Wan So, particularly the popular novels that involve urban and residential spaces of Seoul, as a cultural text that carries a kind of symbolism. It proceeds with the idea that most popular novels reflect the mass phenomena of its times, and that representing real cultural experiences through text, it becomes a means of generalizing the identity shared by the anonymous masses and the characteristics of particular places. Hence the individuality of Park Wan So, who moved to Seoul during the Japanese colonial period and hence forth lived as a middle-class citizen, is inseparable from her literary work. With this attitude and methodology, the paper argues that in the urban space of metropolitan Seoul, the modern ambivalent gaze of the colonial period shifted toward increasingly new value systems, and was replaced by a dichotomous view, and furthermore, that the contents of this dichotomous view has experienced a multivalent transformation through the accumulation of time and the expansion of space.

• BMB Reports
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• v.44 no.6
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• pp.369-374
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• 2011

MHC-I molecules play a critical role in immune surveillance against viruses by presenting peptides to cytotoxic T lymphocytes. Although the mechanisms by which MHC-I molecules assemble and acquire peptides in the ER are well characterized, how MHC-I molecules traffic to the cell surface remains poorly understood. To identify novel proteins that regulate the intracellular transport of MHC-I molecules, MHC-I-interacting proteins were isolated by affinity purification, and their identity was determined by mass spectrometry. Among the identified MHC-I-associated proteins was Tmp21, the human ortholog of yeast Emp24p, which mediates the ER-Golgi trafficking of a subset of proteins. Here, we show that Tmp21 binds to human classical and non-classical MHC-I molecules. The Tmp21-MHC-I complex lacks ${\beta}_2$-microglobulin, and the number of the complexes is increased when free MHC-I heavy chains are more abundant. Taken together, these results suggest that Tmp21 is a novel protein that preferentially binds to ${\beta}_2$-microglobulin-free MHC-I heavy chains.

• Korean Institute of Interior Design Journal
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• v.19 no.1
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• pp.169-179
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• 2010

Since modernization period thru Japanese colonial era the modern architect began to play a important role for Korean housing architecture. So called 'House as an Art Work', detached houses by prominent architects, belong to a meaningful sector in Korean modern housing architecture. The harmony with tradition was always big issue for such works. This study aims to understand how paradigms for tradition were interpreted with the changes of the time. The analysis showed various tryout by architects, that concretize traditional characteristics in their work. For example, traditional lifestyle were clearly reflected in the floorplan during 30's and 40's. In the 70's building mass and formative roof design were emphasized to present traditional image. As well as it represented primitive esthetic and vernacular decoration. In addition, expressive tendency, that demonstrates korean sentiment through material and its texture, got a preference. Since latter half of 80's some traditional architectural elements were modernized and space characteristics were newly created from acculturation. Furthermore the philosophy of "Subdivision of building wings and Emptiness" follows this trend. This type made a courtyard and connected articulated building masses each other. "Sympathy with Nature" were most essential for a traditional houses in contrast with western architecture. Many architects today make various method to bring nature into interior space and to contemplate nature In the house. Such kinds of adaptation to tradition could be understood as a unique process to manifest identity of Korean modern houses.

• Molecules and Cells
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• v.44 no.10
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• pp.746-757
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• 2021

Plant somatic cells can be reprogrammed into a pluripotent cell mass, called callus, which can be subsequently used for de novo shoot regeneration through a two-step in vitro tissue culture method. MET1-dependent CG methylation has been implicated in plant regeneration in Arabidopsis, because the met1-3 mutant exhibits increased shoot regeneration compared with the wild-type. To understand the role of MET1 in de novo shoot regeneration, we compared the genome-wide DNA methylomes and transcriptomes of wildtype and met1-3 callus and leaf. The CG methylation patterns were largely unchanged during leaf-to-callus transition, suggesting that the altered regeneration phenotype of met1-3 was caused by the constitutively hypomethylated genes, independent of the tissue type. In particular, MET1-dependent CG methylation was observed at the blue light receptor genes, CRYPTOCHROME 1 (CRY1) and CRY2, which reduced their expression. Coexpression network analysis revealed that the CRY1 gene was closely linked to cytokinin signaling genes. Consistently, functional enrichment analysis of differentially expressed genes in met1-3 showed that gene ontology terms related to light and hormone signaling were overrepresented. Overall, our findings indicate that MET1-dependent repression of light and cytokinin signaling influences plant regeneration capacity and shoot identity establishment.

• Cross-Cultural Studies
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• v.24
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• pp.19-31
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• 2011

This article examines the historical view of Benito $P{\acute{e}}rez$ $Gald{\acute{o}}s$ in his historical novel named "Zaragoza" and "$C{\acute{a}}novas$" by comparing with Numancia of Cervantes. In front of the national survival crisis, $Gald{\acute{o}}s$ recognizes that it is necessary that Spain should recover humanity and morality on basis of the krausist ideal. The krausists warned subjective idealism, stressing on free-will and moral conscience in a harmonious balance of rationality. Without refusing patriotism, they warned egoistic patriotism deformed by national selfishness or nationalism. Giner de los Ríos insisted on a necessity of harmonizing national inclination and universal one. $Gald{\acute{o}}s$ emphasizes that the identity of Spain is not possession of privileged class but sweat and tears of the mass of people in daily life. He represents Efemera as the ideal history on the basis of Unamuno's 'intrahistoria', by which, instead of $Luk{\acute{a}}cs^{\prime}$ progressive history, we can peep into a historical poetics of human free nature and vividness of discontinuous and endless shifting. According to the sub-history, we come to the conclusion that $Gald{\acute{o}}s$ and Cervantes would lay emphasis on sub-history, aiming to essence of Spanish soul originated from national landscape and people's daily life.