• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.10a
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• pp.343-344
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• 2010

markerless system to a two-dimensional imaging is used to estimate the depth map as a stereo vision system uses expensive equipment. We estimate the depth map from monocular image enhancement and object extracted relative to the vanishing point is estimated depth map. Augmented objects in order to get better virtual immersion depending on the distance of the objects should be drawn in different sizes. In this paper, creating images obtained from the vanishing point, and in-depth information on the augmented object, augmented with different sizes and improved engagement of inter-object interaction.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.8
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• pp.87-94
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• 2018

Hwaseong Fortress was designated as a World Heritage Site by UNESCO in 1997 in recognition of its architectural, cultural, and historical significance. In order to provide a compelling and active experience to the visitors of Hwaseong Fortress more effectively and intuitively, an augmented reality (AR) mobile application is developed. The main objective of this paper is to describe the implementation of AR technology in the developed application; and most importantly, how the application is designed for the visitors to actively and intuitively experience the various architectural and historical contents of Hwaseong. The tour routes and their point of interests were selected carefully after deliberation of their contents. To maximize the user interaction with the application and the architectural contents of Hwaseong, not only the conventional AR technology (marker method), but also the markerless AR method is adopted in the developed application. It is expected that visitors of Hwaseong Fortress enjoy the tour, while learning the architectural, historical, and cultural contents through the active and intuitive interaction with the developed AR mobile application.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1722-1731
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• 2021

The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.619-628
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• 2014

To identify lipase LipA (PFL_0617) from Pseudomonas protegens Pf-5, a lipA deletion mutant (Pf0617) and a complementary strain (Pf0617lipA) were constructed, and their effects on the lipase production were examined. Pf0617 remarkably decreased its whole-cell lipase activity, whereas Pf0617lipA made its whole-cell lipase activity not only restore to wild-type level but also get a further increment. However, the deletion and overexpression of lipA did not affect the extracellular lipase activity. In addition, the unbroken whole cells of these strains were able to catalyze the hydrolysis of membrane-permeable p-nitrophenyl esters, but could not hydrolyze the membrane-impermeable olive oil. These results confirmed that LipA was an intracellular lipase and Pf-5 could also be used as a natural whole-cell biocatalyst. To evaluate the potential of Pf-5 as a whole-cell biocatalyst and separately characterize the whole-cell LipA, the properties of the whole-cell lipases from Pf-5 and Top10lipA were characterized. The results demonstrated that both Pf-5 and Top10lipA exhibited high tolerance to alkaline condition, high temperature, heavy metal ions, surfactants, and organic solvents. Taken together, lipA can realize functional expression in E. coli Top10, and Pf-5 and Top10lipA as whole-cell biocatalysts may have enormous potential in applications.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.45-53
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• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.

• Journal of Korea Multimedia Society
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• v.15 no.8
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• pp.1004-1016
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• 2012

This paper proposes a mobile augmented reality system to provide detail information of the products using QR code included in them. In the proposed system, we perform the estimation of the camera pose using both of marker-based and markerless-based methods. If the camera can see the QR code, we perform the estimation of the camera pose using the set of rectangles in the QR code. However, if the QR code is out of sight, we perform the estimation of the camera pose based homography between consecutive frames. Moreover, the content of the augmented reality in the proposed system is made by using meta-data. Therefore, the user can make contents of various scenario using only meta-data file without modification of our system. Especially, our system maintains the contents as newly updated state by the on-line server. Thus, it can reduce the unnecessary update of the program.

• The Academic Congress of Korean Shoulder and Elbow Society
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• 2009.03a
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• pp.174-174
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• 2009

The acromioclavicular-hook-plate is one of the surgical treatments for distal clavicle fracture and traumatic acromioclavicular (AC) joint dislocation. Although this procedure can obtain rigid and accurate anatomical reduction of the AC joint, secondary widening of the hook-hole in the acromion is often seen during postoperative follow-up. This complication is owing to the high-degree of mobility of the AC joint. Therefore, it is important to evaluate the effect on these complications due to the position of the hook-hole. The purpose of the present study is to investigate three-dimensionally the effect due to the position of the hook-hole during arm abduction motion. We studied in vivo and three-dimensional kinematics of the normal shoulder joint with use of a markerless bone-registration technique. Magnetic resonance images of 14 shoulders of 7 healthy volunteers were acquired in 7 positions between $0^{\circ}$ and $180^{\circ}$ of abduction. We created three-dimensional computer models of the bones and the acromioclavicular-hook-plate. Based on the three-dimensional kinematics data, we simulated the widening of the hook-hole each different positioning of the hook-hole. The widths of the hook-holes almost linearly increased. And these widths significantly increased, when we put the hook-hole on the acromion from AC joint to 20 mm and 25 mm posterior position.

• The Academic Congress of Korean Shoulder and Elbow Society
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• 2009.03a
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• pp.43-43
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• 2009

Glenohumeral ligaments play an important role in stabilizing the shoulder. However, it is impossible to know how they function in vivo during shoulder motion. To help elucidate this stabilizing role, we conducted in vivo three-dimensional kinematics of the normal shoulder joint using a markerless bone-registration technique. Magnetic resonance images of 14 shoulder joints of 7 healthy volunteers were acquired for 7 isometric abduction positions between $0^{\circ}$ and $180^{\circ}$. We then calculated three-dimensional shortest paths between the origin and insertion of each ligament based on anatomical study in each abduction position. At $0^{\circ}$ of abduction, the posterior band of the coracohumeral ligament displayed the maximum length. At $30^{\circ}$ of abduction, the superior glenohumeral ligament displayed the maximum length. At $60^{\circ}$ of abduction, the anterior band of the coracohumeral ligament and the middle glenohumeral ligament displayed the maximum length. At $120^{\circ}$ of abduction, the anterior band of the inferior glenohumeral ligament displayed the maximum length. We think that the maximum length of these results is an important influence on the function of the soft tissue stabilizer.

• Journal of Korea Game Society
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• v.12 no.4
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• pp.45-51
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• 2012

Current virtual game interfaces cannot comprehend our metaphor, cannot reflect on our natural behavior aspect, cannot make us immerse into a game, and makes a barrier between virtual game space and our real behavior. It is very meaningful issue to use real objects tightly related to human-being's behaviors or reactions for interacting with game applications. Interactive Augmented Reality interfaces may augment users' perception of the real world by adding virtual information to it. We attempted an experiment on camera-based non-marker interface for online TCG application. This experiment uses real TCG cards which are recognized by our two phases Image KeyPoint Extraction/Matching Algorithm. These initiative experiments not only enlarge immersion and reality to the game, but also make real and virtual world seamless.

• Journal of the HCI Society of Korea
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• v.10 no.2
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• pp.45-55
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• 2015

In recent years, keypoint recognition and tracking technologies are considered as crucial task in many practical systems for markerless augmented reality. The keypoint recognition and technologies are widely studied in many research areas, including computer vision, robot navigation, human computer interaction, and etc. Moreover, due to the rapid growth of mobile market related to augmented reality applications, several effective keypoint-based matching and tracking methods have been introduced by considering mobile embedded systems. Therefore, in this paper, we extensively analyze the recent research trends on keypoint-based recognition and tracking with several core components: keypoint detection, description, matching, and tracking. Then, we also present one of our research related to mobile augmented reality, named mobile tour guide system, by real-time recognition and tracking of tour maps on mobile devices.