• Asian Pacific Journal of Cancer Prevention
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• v.13 no.1
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• pp.301-304
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• 2012

Objective: To explore the associations of serum tumor associated material (TAM) with other common tumor markers like carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125), carbohydrate antigen19-9 (CA19-9) and its clinical application in non-small cell lung cancer (NSCLC) patients. Methods: A total of 87 patients were enrolled into this study, all with histologically or cytologically confirmed NSCLC. With the method of chemical colorimetry, the level of TAM was determined and compared, while chemiluminescence was used to measure the levels of common tumor markers. Results: The level of TAM decreased after chemotherapy compared with before chemotherapy when CT or MRI scans showed disease control. Furthermore, it increased when disease progessed and there was no statistically significant difference in monitoring of TAM and common tumor markers (P>0.05). Conclusions: Detecting TAM in NSCLC patients has a higher sensitivity and specificity, so it can be used as an indicator for clinical monitoring of lung cancer chemotherapy.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2000.06a
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• pp.107-114
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• 2000

Marine analysis was made to investigate the hydrodynamic effects of a Wing in Ground (WIG) by means of finite difference techniques. The air flow field around WIG is analyzed by the Marker & Cell (MAC) based method, and the interaction between WIG and the free surface are studied by showing pressure distributions above the free surface. In the latter part, computations are extended to make clear the flow characteristics of a high speed catamaran in the rang of Froude numbers 0.2 to 1.0 with a separation to length ratios of 0.2, 0.3 and 0.5. The Navier-Stokes solver is invoked in which the nonlinear free-surface boundary condition is applied. For the validation, computational results are compared with the experiments.

• Journal of Mechanical Science and Technology
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• v.15 no.4
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• pp.465-472
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• 2001

A numerical study is carried out to clarify the characteristics of flow fields and breaking phenomena around a high speed catamaran hull advancing on calm water. Computations are carried out for Froude numbers between 0.2 and 1.0 and for ratios of the distance between hulls to the catamaran length varying between 0.2 and 0.5 for a mathematically defined Wigley hull. A Navier-Stokes solver which includes the nonlinearities of free surface conditions is employed. Computations are performed in a rectangular grid system based on the Marker & Cell method. For validation, present computation results are compared with existing experimental results. As an application, the results of the displacement catamaran are used for the breaking analysis.

• Journal of Mechanical Science and Technology
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• v.15 no.3
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• pp.384-391
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• 2001

The aerodynamic effects of a 3-dimensional Wing in Ground Effect (WIG) which moves above the free surface has been numerically investigated via finite difference techniques. The air flow field around a WIG is analyzed by a Marker & Cell (MAC) based method, and the interactions between WIG and the free surface are studied by the pressure distributions on the free surface. Waves are generated by the surface pressure distribution, and a Navier-Stokes solver has been employed, to include the nonlinearities in the free surface conditions. The pressure values Cp and lift/drag ratio are reviewed by changing the height/chord ratio. In the present computations a NACA0012 airfoil with a span/chord ratio of 3.0 are treated. Through computational results, it is confirmed that the free surface can be treated as a rigid wavy wall.

• Journal of Ocean Engineering and Technology
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• v.6 no.1
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• pp.29-42
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• 1992

자유표면하에 잠긴 복잡한 3차원 물체 주위의 흐름을 해소하기 위한 수치계산법이 TUMMAC(Tokyo Univ. Modified Marker And Cell)법을 기초로 하여 개발되었다. 임의물체의 no-slip 3차원 물체표면조건을 보다 간단히 처리하기 위하여 "porosity"라는 개념이 도입되었으며, 담수성에 잠겨 있는 해저돌출물 주위의 유동을 계산하여 그 응용성을 검토하였다. 돌출물 후방의 복잡한 와동들의 상호간섭이 잘 시뮬레이션 되었다.시뮬레이션 되었다.

• 한국전산유체공학회:학술대회논문집
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• 1998.11a
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• pp.175-184
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• 1998

본 연구에서는 제한된 수로를 운항하는 선체주위의 유동특성에 관한 연구를 수행하고자 하였다. 일반적으로 운하 또는 하천을 운항하는 선박의 경우 제한된 수심의 영향으로 천수효과가 발생하게 된다. 이러한 천수의 효과와 제한된 폭의 영향으로 선수부분에서 선체보다 선행하는 파도가 전파되어 나아가기도 하며, 이로 인하여 선박은 보다 많은 조파저항을 받게 된다. 본 연구에서는 임의의 형상을 갖는 선체가 폭과 수심이 제한된 운하를 임계속도 근처에서 운항하는 경우에 관하여 폭과 수심을 변화시켜가며 수치계산을 수행하여, 제한수로에서의 임의의 선체주위의 유동특성을 관찰하고자 하였다. 수치계산은 MAC(Marker And Cell)법을 기초로 한 유한차분법(Finite Difference Method)을 사용하였으며, 계산에 사용된 격자계는 임의의 형상에 관하여 격자생성이 용이한 직사각형 격자계(Rectangular Grid System)를 사용하였다.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2001.10a
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• pp.202-206
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• 2001

A three dimensional incompressible Navier-Stokes code based on the third derivative upwind is employed to simulate the flow around the underwater vehicle advancing on the calm water. Computations are carried out in the range of Froude numbers 0.4 to 0.7. The wave resistance, lift, moment and the pressure distribution on the body are calculated. Computations are performed in a rectangular grid system based on the Marker & Cell method. For validation, computation results are compared with existing experimental results.

• BMB Reports
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• v.38 no.1
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• pp.41-48
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• 2005

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, which could infect Hz-AM1 cells and generate recombinant HaNPV (rHa-Bar) containing the barnase gene, a ribonuclease gene from Bacillus amyloliquefaciens. Since the expression vector carrying barnase gene cannot replicate in the absence of barstar, a specific inhibitor of barnase, we constructed a new cotton bollworm cell line (AM1-NB) using the marker rescue method. In AM1-NB barstar was integrated into the cellular chromosome to sustain the replication of rHa-Bar. To screen out recombinant HaNPV for potential use as biopesticide, Hz-AM1 and AM1-NB cell lines were infected with rHa-Bar, respectively. The results obtained indicate that Viral progenies in AM1-NB were 23 and 160 times greater than those in Hz-AM1 48 h and 72 h after infection, respectively. With additional insertion of the polyhedron gene from AcNPV (Autographa californica nuclear polyhedrosis virus) into the Hanpvid genome, rHa-Bar regained the polyhedron phenotype and its pest-killing rate greatly improved. Toxic analysis showed that the lethal dosages ($LD_{50}$) and the lethal time(s) ($LT_{50}$) of rHa-Bar were reduced by 20% and 30%, respectively, compared to wt-HaNPV in the third instar larvae of cotton bollworm. This study shows that in AM1-NB barnase can be effectively produced and used as pest-killing agent for the biological control of cotton pests.

• International Journal of Oral Biology
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• v.34 no.4
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• pp.177-183
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• 2009

Human mesenchymal stem cell (hMSCs) isolated from human adult bone marrow have self-renewal capacity and can differentiate into multiple cell types in vitro and in vivo. A number of studies have now demonstrated that MSCs can differentiate into various neuronal populations. Due to their autologous characteristics, replacement therapy using MSCs is considered to be safe and does not involve immunological complications. The basic helix-loop-helix (bHLH) transcription factor Olig2 is necessary for the specification of both oligodendrocytes and motor neurons during vertebrate embryogenesis. To develop an efficient method for inducing neuronal differentiation from MSCs, we attempted to optimize the culture conditions and combination with Olig2 gene overexpression. We observed neuron-like morphological changes in the hMSCs under these induction conditions and examined neuronal marker expression in these cells by RTPCR and immunocytochemistry. Our data demonstrate that the combination of Olig2 overexpression and neuron-specific conditioned medium facilitates the neuronal differentiation of hMSCs in vitro. These results will advance the development of an efficient stem cell-mediated cell therapy for human neurodegenerative diseases.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.8
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• pp.4679-4683
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• 2013

Background: ERCC1 is considered as a promising molecular marker that may predict platinum based chemotherapy response in non small cell lung cancer patients. We therefore investigated whether its expression is indeed associated with clinical outcomes in advanced stage NSCLC patients. Materials and Methods: Pretreatment tumor biopsy samples of 83 stage 3B and 4 non-small cell lung cancer patients treated with platinum based chemotherapy were retrospectively analyzed for immunohistochemical ERCC1 expression. None of the patients received curative surgery or radiotherapy. Results: By calculating H- scores regarding the extent and intensity of immunohistochemical staining of tumor biopsy samples, ERCC1 expression was found to be positive in 50 patients (60.2%). ERCC1 positive and negative groups had no statistically significant differences regarding treatment response, progression free survival and overall survival (respectively p=0.161; p=0.412; p=0.823). Conclusions: In our study we found no association between ERCC1 expression and survival or treatment response. The study has some limitations, such as small sample size and retrospective analysis method. There is need of more knowledge for use of ERCC1 guided chemotherapy regimens in advanced stage NSCLC.