• 대한세포병리학회지
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• 제12권2호
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• pp.89-95
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• 2001

The cytological distinction of carcinoma cells from reactive mesothelial cells in serous effusions nay be difficult or imposslble based on morphology alone, especially In specimens containing reactive mesothelial cells which form glandular or ball- or papillary-shaped conglomerates or which mimic malignant nuclear features. Calretinin is a newly reported immunocytochemical marker for mesothelial cells, which can potentially be utilized for facilitating this distinction. This study evaluated the usefulness of calretinin for the discrimination between reactive mesothelial and metastatic carcinoma cells in serous effusion. Immunocytochemical staining was undertaken on 33 benign reactive and 87 malignant serous effusion specimens with histologically confirmed diagnoses. The specimens including smears and cell blocks were stained with polyclonal antibody to calretinin by labelled streptavidin-biotin method. The positive expression of calretinin was noted In 32(97.0%) of 33 benign reactive effusions and 9(10.3%) of 87 malignant effusions. The sensitivity and specificity of the calretinin immunostaining for reactive mesothelial cells was 97.0% and 89.7%, respectively. In conclusion, calretinin is a useful marker for distinguishing between reactive mesothelial cells and carcinoma cells in serous effusions.

• Journal of Gastric Cancer
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• 제13권1호
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• pp.69-72
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• 2013

Carcinosarcoma is a rare malignant, biphasic tumor comprised of carcinoma and sarcoma components. In the gastrointestinal tract, carcinosarcoma is most frequently seen in the esophagus and rarely in the stomach. We report a 51-year-old female patient with 2-month-history of epigastric pain and dyspepsia. Endoscopic finding revealed a huge ulcerative lesion that infiltrated from the antrum to the mid-body. An endoscopically taken biopsy revealed poorly differentiated malignant round cell neoplasm. After the palliative subtotal gastrectomy, immunohistochemical studies showed two positive reactions for the epithelial marker and mesenchymal marker. Based on the above findings, the patient was diagnosed with gastric carcinosarcoma. The immunohistochemical analysis is a critical method in making an accurate diagnosis of carcinosarcoma.

• Biomolecules & Therapeutics
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• 제15권4호
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• pp.261-265
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• 2007

Hydroxyproline (HYP) is a post-translational product of proline hydroxylation catalyzed by an enzyme prolyl 4-hydroxylase (P4H) which plays a crucial role in the synthesis of all collagens. Considering the role of collagen and its significance in many clinically important diseases such as liver fibrosis, a great deal of attention has been directed toward the development of an assay at cell-based system. The reason is that cell-based assay system is more efficient than enzyme-based in vitro system and takes much less time than in vivo system. Several assay procedures developed for P4H are laborious, time-consuming and not feasible for the massive-screening. Here, we report the cell-based assay method of prolyl 4-hydroxylase in immortalized rat hepatic stellate HSC-T6 cells. To optimize the cell culture condition to assay for HYP content, various concentrations of reagents were treated for different times in HSC-T6 cells. Our data showed that the treatment with ascorbate in a hypoxic condition for 24 h resulted in the maximal increase of HYP by 1.8 fold. Alternatively, cobalt chloride ($5\;{\mu}M$) and ascorbate ($50\;{\mu}M$) in normoxic states exhibited similar effect on the production of HYP as in a hypoxic condition. Therefore, cobalt chloride can be substituted for a hypoxic condition when an anaerobic chamber is not available. Rosiglitazone and HOE077, known as inhibitors of collagen, synthesis decreased P4H enzyme activity by 32.3% and 15%, respectively, which coincided with previous reports from liver tissues. The level of the smooth muscle ${\alpha}$-actin, a marker of activated stellate cells, was significantly increased under hypoxia, suggesting that our experimental condition could work for screening the anti-fibrotic compounds. The assay procedure took only 3 days after treatment with agents, while assays from the primary stellate cells or liver tissues have taken several weeks. Considering the time and expenses, this assay method could be useful to screen the compounds for the inhibitor of prolyl 4-hydroxylase.

• Asian-Australasian Journal of Animal Sciences
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• 제29권1호
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• pp.23-28
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• 2016

The Fas (APO-1, TNFRSF6) gene known as a member of the tumor necrosis factor receptor superfamily was selected for DNA marker development in Korean cattle. It is a cell membrane protein and mediates programmed cell death (apoptosis). We discovered single nucleotide polymorphisms (SNPs) within Fas gene in order to develop novel DNA markers related to economical traits at the genomic level. The sequences of whole exon and 1 kb range of both front and back of the gene were determined by direct-sequencing methods using 24 cattle. A total of 55 SNPs were discovered and we selected 31 common polymorphic sites considering their allele frequencies, haplotype-tagging status and linkage disequilibrium (LD) for genotyping in larger-scale subjects. The SNPs were confirmed genotype through the SNaPshot method (n = 274) and were examined for a possible genetic association between Fas polymorphisms and marbling score. So, the SNPs that were identified significant are g.30256G>C, g.31474C>A, g.31940A>G, and g.32982G>A. These results suggest that SNPs of Fas gene were associated with intramuscular fat content of meat quality traits in Korean cattle.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제23권2호
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• pp.93-114
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• 2019

In this article, we introduce a finite difference method for solving the Navier-Stokes equations in rectangular domains. The method is proved to be energy stable and shown to be second-order accurate in several benchmark problems. Due to the guaranteed stability and the second order accuracy, the method can be a reliable tool in real-time simulations and physics-based animations with very dynamic fluid motion. We first discuss a simple convection equation, on which many standard explicit methods fail to be energy stable. Our method is an implicit Runge-Kutta method that preserves the energy for inviscid fluid and does not increase the energy for viscous fluid. Integration-by-parts in space is essential to achieve the energy stability, and we could achieve the integration-by-parts in discrete level by using the Marker-And-Cell configuration and central finite differences. The method, which is implicit and second-order accurate, extends our previous method [1] that was explicit and first-order accurate. It satisfies the energy stability and assumes rectangular domains. We acknowledge that the assumption on domains is restrictive, but the method is one of the few methods that are fully stable and second-order accurate.

• 한국가금학회지
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• 제16권1호
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• pp.1-8
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• 1989

유전물질의 총합체인 염색체의 분리와 분석을 정확히 하여 유전인자의 위치를 밝히고 또한 유전자조작 기술을 이용할 수 있는 방법을 구명하여 닭의 능력개양을 꾀하기 위해서 실시된 본 연구의 결과를 요약하면 아래와 같다. 1. 백혈구 배양을 통하며 염색체를 분리하고 Giemsa banding을 통한 염색체 분석에서 1번 염색체의 경우 20층에 달하는 banding pattern을 발견할 수 있는 정확한 분석으로 1∼9번, 성염색체의 정상 banding pattern을 밝힐 수 있었고 C-banding의 결과 모든 염색체에서 유전자 작용이 없는 constitutive heterochromatin의 위치를 밝힐 수 있었다. 2. 유전자 조작 기술 중 중요한 단계인 genetic vector로서 닭의 원시생식세포(Primodial germ cell, PGC)를 이용하기 위하여 genetic marker로서 3배체의 염색체를 가친 PGC를 정상 host embryo에 이식시켜서 성장중인 host embryo의 성선에서 donor PGC의 genetic marker(3n)가 발견됨으로써 PGC를 이용한 가금의 유전자 조작이 가능함을 밝혔다.

• Transactions on Control, Automation and Systems Engineering
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• 제4권3호
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• pp.224-230
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• 2002

In this paper, a counting algorithm hybridized with an adaptive automatic thresholding method based on Otsu's method and the algorithm that elongates markers obtained by the well-known watershed algorithm is proposed to enhance the exactness of the microcell counting in microscopic images. The proposed counting algorithm can be stated as follows. The transformed full image captured by CCD camera set up at microscope is divided into cropped images of m$\times$n blocks with an appropriate size. The thresholding value of the cropped image is obtained by Otsu's method and the image is transformed into binary image. The microbial cell images below prespecified pixels are regarded as noise and are removed in tile binary image. The smoothing procedure is done by the area opening and the morphological filter. Watershed algorithm and the elongating marker algorithm are applied. By repeating the above stated procedure for m$\times$n blocks, the m$\times$n segmented images are obtained. A superposed image with the size of 640$\times$480 pixels as same as original image is obtained from the m$\times$n segmented block images. By labeling the superposed image, the counting result on the image of microbial cells is achieved. To prove the effectiveness of the proposed mettled in counting the microbial cell on the image, we used Acinetobacter sp., a kind of ammonia-oxidizing bacteria, and compared the proposed method with the global Otsu's method the traditional watershed algorithm based on global thresholding value and human visual method. The result counted by the proposed method shows more approximated result to the human visual counting method than the result counted by any other method.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 1995년도 추계 학술대회논문집
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• pp.43-48
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• 1995

A numerical computation is carried out to analyse characteristics of flow fields around Air Supported Ships having arbitrary form. The computations are performed in a rectangular grid system with MAC(Marker And Cell) method. The governing equations are represented in a Finite Difference form by forward differencing in time and centered differencing in space except for convection terms. For validation of this numerical analysis method, the computation of flow fields around Catamaran and ACV(Air Cushion Vehicle) with pressure distribution on free surface are done, and that around Surface Effect Ship is also carried out. The results of the computations are compared with the those of existed numerical computation and experimental results with the same condition.

• 한국주조공학회지
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• 제12권1호
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• pp.40-50
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• 1992

A computer simulation of mold filling has been performed in order to analyze the fluid flow pattern in a mold cavity since casting defects such as cold shut formation, entrapment of air or gas, and inclusions are closely related to the fluid flow phenomena. The flow of molten metal entering the mold cavity with free surface has been modeled by SMAC(Simplified Marker and Cell) method. Two dimensional analysis was carried out on plate shape castings with two types of gate system. The calculation results were compared with those of water modeling experiments and showed relatively good agreement.

• 대한조선학회지
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• 제25권4호
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• pp.13-27
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• 1988

TUMMAC차분법(差分法)이 두 가지의 삼차원파(三次元波), 즉 배의 항주(航走)에 따른 파형(波形)과 수직사각주(垂直四角柱) 주위의 쇄파해석(碎波解析)에 적용(適用)되어졌다. Series 60($C_B=0.6$) 모형선(模型船)과 HSVA 모형선(模型船)의 선수부(船首部)에 의한 파형(波形)이 자유표면(自由表面)과 선체후반부(船體後半部)에서의 boundary cell 처리를 엄밀화한 $TUMMAC-IV_{vm1}$방법(方法)에 의하여 계산(計算)되어졌으며, 그 결과가 실제(實際)의 실험결과(實驗結果)들과 비교(比較)되었다. 실험결과(實驗結果)와의 비교를 통하여, $TUMMAC-IV_{vm1}$방법(方法)은 배에 의한 비선형파(非線形波)의 특성해석(特性解析)에 매우 응용성(應用性)이 높음을 보였다. 특히, 비대선형(肥大船型)인 HSVA 모형선(模型船)의 경우는 계산(計算)된 비선형선수파(非線形船首波)의 특성(特性)이 실험결과(實驗結果)와 좋은 일치를 보였다. 수직사각주(垂直四角柱)주위의 쇄파(碎波)를 포함한 심한 비선형파(非線形波)가 삼차원비선형파(三次元非線形波)의 대표적(對表的)인 예(例)로써 TUMMAC-VI 방법(方法)에 의하여 계산(計算)되었다. TUMMAC-VI 방법(方法)은 이층류(二層流)를 함께 푸는 방법(方法)으로, 두가지 유체(流體) 사이의 밀도변화(密度變化), 즉 marker-density를 이용하여 interface를 결정하게 된다. 그러나, 비압축성유체(非壓縮性流體)에 관한 N-S 방정식(方程式)의 해(解)이므로, 그 밖의 계산(計算)에서는 각각의 대표적(對表的)인 밀도(密度)를 사용한다. 계산결과(計算結果)는 삭가주(四角柱) 앞부분의 원형파(圓形波)와 앞어깨에서의 심한 경사를 가지는 파(波), 뒷부분에서의 복잡(複雜)한 쇄파현상(碎波現像)들이 실제의 파특성(波特性)을 잘 나타내어 주었다. TUMMAC-VI 방법(方法)은 interface의 처리를 포함하여 앞으로도 개선(改善)의 여지가 많으며, 단지 공기(空氣)와 물만이 아닌 일반적(一般的)인 이층류(二層流)의 해석에도 폭 넓게 이용될 수 있으리라 본다.