• 제목/요약/키워드: Marine natural product

검색결과 137건 처리시간 0.028초

재조합 Agrobacterium tumefaciens NTL4 균주를 이용한 quorum Sensing Autoinducer 검색에 용매와 계면활성제가 미치는 영향 (Effect of Solvents and Surfactants on the Whole-cell Bioassay for Screening Quorum Sensing Autoinducers Using the Recombinant Agrobacterium tumefaciens NTL4 Strain)

  • 고경표;김연희;김정선;박성훈
    • 한국해양바이오학회지
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    • 제1권4호
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    • pp.260-267
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    • 2006
  • 재조합 Agrobacterium tumefaciens균주를 사용한 Liquid Culture Assay는 quorum sensing autoinducer를 검색하는 생물학적 분석 방법으로 개발되었다. 그러나 이 시스템에 사용되는 해양천연물 시료들이 일반적으로 낮은 수용액에 대한 용해도를 갖기 때문에 활성검색에 걸림돌이 되고 있다. 시료의 용해도는 유기용매 혹은 계면활성제의 첨가로 증가될 수 있으나, 유기용매나 계면활성제 자체가 검색결과의 정확한 해석을 방해할 수 있으므로 적절한 조건의 확립이 매우 중요하다. Methanol, ethanol, 1-propanol, DMSO와 DMF를 0~10% 농도 범위에서 재조합 A. tumefaciens균주에 대한 세포 성장에 미치는 영향과 ${\beta}$-galactosidase activity에 미치는 영향을 살펴본 결과 methanol 2%이하의 농도가 가장 적합하였으며, 계면활성제의 경우, Tween 20, Tween 80보다는 Triton X-100이 약 0.05% 농도에서 세포내로의 활성물질의 전달효율을 높이는데 효과적이었다.

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기후변화가 마로해의 김 양식에 미치는 영향 및 대응방안 (Effects of Climate Change on Purple Laver Farming in Maro-hae (Jindo-gun and Haenam-gun), Republic of Korea and Countermeasures)

  • 김태형;신종암;최상덕
    • 수산경영론집
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    • 제52권2호
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    • pp.55-67
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    • 2021
  • Global warming affects critical natural resources, one of which is the oceans that occupy 70% of the total cover of the earth. In other words, ocean warming is a subset of global warming which needs to be addressed urgently. Purple laver (pyropia spp.) is one of the most vulnerable items to climate change although it is a major export product of Korean fisheries. The purpose of this study is to analyze the causality of how climate change caused by global warming affects the increase or decrease of PLP (purple laver production). The target area for analysis was set to Maro-hae between Jindo-gun and Haenam-gun. We selected marine environmental factors and meteorologic factors that could affect PLP as variables, as well as co-integration tests to determine long-term balance, and the Granger causticity tests. As a result, PLP and marine environmental factors WT (water temperature), pH, and DO confirmed that long-term equilibrium relationships were established, respectively. However, there is only causality with WT and it is confirmed that there is only a correlation between pH and DO (dissolved oxygen). There was no long-term equilibrium relationship between PLP and HDD (heating degree days) and there is a causal effect that HDD affects PLP; however, it was less clear than that of WT. The relationship between PLP and RF (rainfall), WS (wind speed), SS (percentage of sunshine), and FF (farm facilities) was all balanced in the long term, and causality exists. Based on the results of the analysis, policy proposals were made.

공간적인 알리아싱을 포함한 탄성파 트레이스의 내삽을 위한 요소파 기반의 Matching Pursuit 기법 (Wavelet Based Matching Pursuit Method for Interpolation of Seismic Trace with Spatial Aliasing)

  • 최지훈;변중무;설순지
    • 지구물리와물리탐사
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    • 제17권2호
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    • pp.88-94
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    • 2014
  • 탄성파 탐사자료의 획득 시, 기계적인 결함이나 지형적인 접근성 등으로 인하여 자료를 부분적으로 획득하지 못하거나, 해상 탐사의 경우 스트리머의 간격으로 인하여 공간적인 알리아싱(aliasing)이 발생할 수 있다. 이러한 불규칙하고 공간적인 알리아싱이 존재하는 자료는 추후 탄성파 자료처리과정에서 문제를 일으키게 된다. 만약, 정확하고 효율적인 내삽(interpolation) 기법을 보유하고 있다면, 이러한 문제를 해결할 수 있으며, 나아가 탐사를 수행할 때 송신원 또는 수신기의 수를 줄일 수 있으므로 탐사의 시간 및 비용이 줄어들게 된다. 여러 내삽 방법들 중 Matching Pursuit 기법을 이용한 내삽 방법은 고르기(sampling)의 형태와 상관없이 적용이 가능하다는 장점을 가지고 있다. 그러나 정현파 함수(sinusoidal function)을 기저함수로 이용하는 경우, 공간적인 알리아싱이 존재하는 탄성파자료에 적용시 정확한 내삽이 불가능하다는 한계를 지니고 있다. 따라서 본 연구에서는 공간적인 알리아싱의 문제를 해결하기 위하여, 정현파 함수가 아닌 요소파(wavelet)를 기저함수로 이용하는 요소파 기반의 Matching Pursuit 기법을 개발하였다. 또한, L-2 norm을 이용한 기존의 방법과 달리, 내적을 이용하여 내삽의 수행속도를 향상시켰다.

Anti-MRSA action of Papenfussiella kuromo

  • Lee, Sun-Ae;Mun, Su-Hyun;Kang, Ok-Hwa;Joung, Dae-Ki;Seo, Yun-Soo;Kang, Da-Hye;Kim, Sung-Bae;Kong, Ryong;Yang, Da-Wun;Kwon, Dong-Yeul
    • Natural Product Sciences
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    • 제20권1호
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    • pp.39-43
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    • 2014
  • Papenfussiella kuromo (PK) is a marine plant and an abundant ecological resource for the future; it is found in almost 80% of the terrestrial biosphere. The aim of this study was to investigate the antibacterial activity of PK against methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant pathogen. The minimum inhibitory concentrations (MICs) of PK hexane fraction (PKH) against 7 strains of MRSA ranged from 1.0 to 2.0 mg/mL. In the checkerboard dilution method, a synergistic effect of the PKH and the antibiotics (oxacillin and norfloxacin) was seen. PKH markedly reduced the MIC of each of the 4 antibiotics against MRSA. The time-kill assay showed that the synergistic activity of PKH and an antibiotic reduced the bacterial counts below the lowest detectable limit after 24 h. These findings suggest that PKH has antibacterial activity, and may be important baseline data in future extensive studies of living marine resources as a source of compounds active against MRSA.

Gene Cloning, Expression, and Characterization of a $\beta$-Agarase, AgaB34, from Agarivorans albus YKW-34

  • Fu, Xiao Ting;Pan, Cheol-Ho;Lin, Hong;Kim, Sang-Moo
    • Journal of Microbiology and Biotechnology
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    • 제19권3호
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    • pp.257-264
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    • 2009
  • A $\beta$-agarase gene, agaB34, was functionally cloned from the genomic DNA of a marine bacterium, Agarivorans albus YKW-34. The open reading frame of agaB34 consisted of 1,362 bp encoding 453 amino acids. The deduced amino acid sequence, consisting of a typical N-terminal signal peptide followed by a catalytic domain of glycoside hydrolase family 16 (GH-16) and a carbohydrate-binding module (CBM), showed 37-86% identity to those of agarases belonging to family GH-16. The recombinant enzyme (rAgaB34) with a molecular mass of 49 kDa was produced extracellularly using Escherichia coli $DH5{\alpha}$ as a host. The purified rAgaB34 was a $\beta$-agarase yielding neoagarotetraose (NA4) as the main product. It acted on neoagarohexaose to produce NA4 and neoagarobiose, but it could not further degrade NA4. The maximal activity of rAgaB34 was observed at $30^{\circ}C$ and pH 7.0. It was stable over pH 5.0-9.0 and at temperatures up to $50^{\circ}C$. Its specific activity and $k_{cat}/K_m$ value for agarose were 242 U/mg and $1.7{\times}10^6/sM$, respectively. The activity of rAgaB34 was not affected by metal ions commonly existing in seawater. It was resistant to chelating reagents (EDTA, EGTA), reducing reagents (DTT, $\beta$-mercaptoethanol), and denaturing reagents (SDS and urea). The E. coli cell harboring the pUC18-derived agarase expression vector was able to efficiently excrete agarase into the culture medium. Hence, this expression system might be used to express secretory proteins.

Inhibitory Effects of Marine Algae Extract on Adipocyte Differentiation and Pancreatic Lipase Activity

  • Kim, Eun-Sil;Lee, Kyoung-Jin;Oh, Kyoung-Hee;Ahn, Jong-Hoon;Kim, Seon-Beom;Liu, Qing;Hwang, Bang-Yeon;Lee, Mi-Kyeong
    • Natural Product Sciences
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    • 제18권3호
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    • pp.153-157
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    • 2012
  • Obesity, which is characterized by excessive fat accumulation in adipose tissues, occurs by fat absorption by lipase and sequential fat accumulation in adipocyte through adipocyte differentiation. Thus, inhibition of pancreatic lipase activity and adipocyte differentiation would be crucial for the prevention and progression of obesity. In the present study, we attempted to evaluate anti-adipogenic activity of several algae extracts employing preadipocytes cell line, 3T3-L1 as an in vitro assay system. The effects on pancreatic lipase activity in vitro were also evaluated. Total methanolic extracts of Cladophora wrightiana and Costaria costata showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Related to pancreatic lipase, C. wrightiana and Padina arborescens showed significant inhibition. Further fractionation of C. wrightiana, which showed the most potent activity, suggested that $CHCl_3$ and n-BuOH fraction are responsible for adipocyte differentiation inhibition, whereas n-BuOH and $H_2O$ fraction for pancreatic lipase inhibition. Our study also demonstrated that n-BuOH fraction was effective both in early and middle stage of differentiation whereas $CHCl_3$ fraction was effective only in early stage of differentiation. Taken together, algae might be new candidates in the development of obesity treatment.

부산물을 이용한 꽃게 통발용 미끼의 유인 효과 (Attracting effect of baits used the by-product for swimming crab Portunus trituberculatus pots)

  • 장호영;구재근;이근우;조봉곤;정병곤
    • 수산해양기술연구
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    • 제44권4호
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    • pp.282-293
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    • 2008
  • In order to develop the substitutive materials for natural baits of swimming crab pots, the attracting effects of swimming crab such as the preference of baits which were made of the by-products of marine and stock raising through the water tank experiments and fishing experiments. On the investigation of mean entrapped catch number to the pot by the baits after putting the 4 kinds of baits, mackerel(M), mackerel with grinded mackerel s internals($MM_I$), mackerel with tuna s internals$MM_I$) and makerel with grinded krill(MK) each in one pot by turns, $MM_I$ and MK were entrapped mean 3.9(13.0%) and they were a little more comparing to M, and $MT_I$ is least with mean 2.1(7.0%)(F=12.913, P < 0.05). Otherwise, on the preference investigation of swimming crabs by the baits after putting the 4 kinds of baits in the 4 pots each, M was entrapped mean 3.0(10%), but $MM_I$, $MT_I$ and MK were mean 1.2(4.0%), 1.0(3.3%) and 1.5(5.0%) each and they were only 30-50% of M(F=13.398, P < 0.05). On the preference investigation of swimming crabs by the 5 kinds of baits, mackerel(M), and krill(K), manila clam($M_C$), pig s fat($P_F$) and chicken s head($C_H$) which were used in substitutive baits, M was entrapped mean 3.2(10.7%), but K was about 50% of catch of M with mean 1.6(5.3%), and $M_C$, $P_F$ and $C_H$ were very few with mean 0.1-0.2(0.3-0.7%)(F=89.186, P < 0.05). On the preference investigation of swimming crabs by the pots which were put each the 3 kinds of baits, original krill(K), grinded krill with gluten and soybean oil cake($K_GGS$) and grinded krill with gluten, soybean oil cake and glycine($K_GGSG_L$) in the blue fluorescent hexahedral plastic bait cages(BF), and which were put the mackerel(M) in the non-fluorescent hexahedral red plastic bait cage($RF_N$), it was entrapped mean 3.0(10.0%) in the pot which was put the mackerel in the $RF_N$, and the same level in the pots which were put the K and $K_GGSG_L$ in the BF, but it was mean 2.0(6.7%) in the pots which was put the $K_GGS$ in BF and it was decreased by 30% of catch comparing to $RF_N$(F=3.750, P < 0.05). On the preference investigation of swimming crab by the pots which was put grinded tuna with gluten, soybean oil cake and glycine($T_IGSG_L$) in the blue fluorescent hexahedral plastic bait cage(BF), and which was put mackerel(M) in the nonfluorescent hexahedral red plastic bait cage($RF_N$), it was entrapped mean 3.3(11.0%) in the pot which was put mackerel in $RF_N$, and mean 2.7(9.0%) in the pot which was put $T_IGSG_L$ in BF and it was about 15% less comparing to use bait M(t=1.387, P < 0.05). As a results of fishing experiments, a plan for enhancing catching efficiency of $T_IGSG_L$ will be required because catching efficiency of $T_IGSG_L$, alternative bait, was half of fish catching efficiency of natural bait using mackerel. Fishing experiments were conducted 3 times using reinforced substitutive artificial bait that is reinforced attractive effect of $T_IGSG_L$ and composed of tuna intestine, grinded mackerel, gluten, soybean cake, glycine and alanine($T_IM_GGSG_LA$). Catching efficiency of $T_IM_GGSG_LA$ was about 80% of that of natural bait made of mackerel.

An Acetophenone Derivative, Clavatol, and a Benzodiazepine Alkaloid, Circumdatin A, from the Marine-Derived Fungus Cladosporium

  • Yang, Guohua;Nenkep, Viviane N.;Siwe, Xavier N.;Leutou, Alain S.;Feng, Zhile;Zhang, Dahai;Choi, Hong-Dae;Kang, Jung-Sook;Son, Byeng-Wha
    • Natural Product Sciences
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    • 제15권3호
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    • pp.130-133
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    • 2009
  • The crude extract of the mycelium of Cladosporium was found to exhibit antimicrobial activity against the Staphylococcus aureus, methicillin-resistant S. aureus, and multidrug-resistant S. aureus. Bioassayguided fractionation of an organic extract led to the isolation of an acetophenone derivative, clavatol (2',4'-dihydroxy-3',5'-dimethylacetophenone) (1), and a benzodiazepine alkaloid, circumdatin A (2). Compound 1 showed moderate antibacterial activity against S. aureus, methicillin-resistant S. aureus, and multidrug-resistant S. aureus with minimum inhibitory concentration (MIC) values of 62.5, 62.5, 31.0 $\mu$g/mL, respectively, but compound 2 was inactive. Compounds 1 and 2 exhibited UV-A protection activity with ED$_{50}$ values of 227.0 and 82.0 $\mu$M, respectively, indicating that they were more potent than the positive control, oxybenzone (ED$_{50}$ 350 $\mu$M), a common sunscreen agent.

현대 서양복식에 표현된 중세 문장의 상징성에 관한 연구 (A Study on the Symbolic Characteristics of Medieval Heraldry in the Modern Fashion)

  • 강림아;이효진
    • 복식
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    • 제44권
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    • pp.153-167
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    • 1999
  • The purpose of this study was to supply the opportunity of being used as a material which can predict future fashion. This study had been divided the symbolic meaning into three parts. The first part was the symbolic expressions in the materials of modern fashion, and the second part was symbolic meaning which was expressed in the accessories, and the last part was the symbolic meaning which was expressed in the physical ornament. The results could be summarized as follows. First, among the forms of heraldry were expressed in modern Fashion, were such as animals like a lion and eagle, which evoked the powerful and lively mood. A vegetable heraldry was expressed on a pattern of textile fabric to pursue the beauty of dress, represented female and classical beauty. A inanimate object heraldry was included all natural material that didn't have all lives the world, represented the mystery and intensity of nature as using the design that sets off the idea through color and figure. Second, in the case of being the forms of heraldry used as a mark or logo in modern fashion, it was visualized the fashion of traditional sense, marine look and military look. This mark or logo had important characteristics of symbolic in that the product had a special meaning and function by trade mark. Third, tattos were used to heighten the people's union in the various subordinate cultures. By ornamenting their bodies, they expressed their special identity and consciousness. Tattos represented a unique menas, which effected a mood of fashion.

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각종 폐수로부터 중금속 이온을 제거하기 위한 Epichlorohydrine-가교키토산의 제조 및 가교도의 효과 (Preparations of the Cross-linked Chitosan Based on a Marine Natural Product with Epichlorohydrine for the Exclusion of Heavy Metal Ions from the Various Wastewater and Its Effect of Crosslinking Ratio)

  • 박영미;전동원
    • 한국의류산업학회지
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    • 제8권5호
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    • pp.577-584
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    • 2006
  • The binding of heavy metal ions onto cross-linked chitosan in dilute aqueous solution has been investigated as a function of pH (4.0 and 7.0), agitation period (10-180min) and concentration of various metal cations (5, 10, 50 and 100ppm). In order to obtain adsorbents that are insoluble and stable, and prevent the dissolution loss of chitosan into an acidic aqueous solution, chitosan flakes were cross-linked with epichlorohydrine (ECH) and its adsorption behavior was compared with that of the non cross-linked chitosan. An advantage of ECH is that it does not eliminate the cationic amine functional group of chitosan. In terms of adsorption ratio, the chitosan cross-linked at an ECH was inferior to original chitosan and was found that chitosan has a selectivity much remarkable than the cross-linked chitosan in low concentrated metal solutions. However, no significant decreases in the adsorption ratios were observed between the cross-linked ECH-chitosan and the non cross-linked chitosan concerning the adsorption of $Ni^{2+}$, $Co^{2+}$, $Pb^{2+}$ and $Zn^{2+}$ acidic solution.