• KSBB Journal
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• 제26권4호
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• pp.341-346
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• 2011

This work is focused on the possibility of marine biomass Codium fragile as renewable resources for production of levulinic acid. In an effort to optimize the reaction conditions of levulinic acid production from Codium fragile, response surface methodology was applied. A total of 18 individual experiments were designed to investigate the effect of reaction temperature, catalyst amount, and reaction time. As a result, 4.26 g/L levulinic acid from Codium fragile was produced in the condition of $160.7^{\circ}C$ of reaction temperature, 3.9% of sulfuric acid, and 39.1 min of reaction time. This result will provide the useful information for chemical production from marine resource.

• Journal of Species Research
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• 제11권4호
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• pp.335-342
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• 2022

Through the 'Discovery of Korean Indigenous Species' project hosted by the National Institute of Biological Resources (NIBR), new or unrecorded species of various protozoans inhabiting the Korean Peninsula have been discovered. Samples were collected from marine, freshwater, and soil habitats in South Korea and all species were identified and reviewed by experts working on the respective taxonomic group. This study includes 17 unrecorded Korean protozoan species discovered through this project, which belong to four phyla: Amoebozoa Lühe, 1913, Cercozoa Cavalier-Smith, 1998, Euglenozoa Cavalier-Smith, 1981, and Ciliophora Doflein, 1901. Among them, three families (Rhogostomidae Dumack et al., 2017, Parauronematidae Small and Lynn, 1985, and Cyclidiidae Ehrenberg, 1838) and three genera (Rhogostoma Belar, 1921, Parauronema Thompson, 1967, and Cyclidium Müller, 1773) were reported for the first time in Korea. Unlike the previous paper of 2017, this study provides a comprehensive taxonomic account of each species (e.g., species name, collection site, synonyms, specimen vouchers, diagnoses, and figures). Additionally, all the species were assigned Korean names.

• 한국어류학회지
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• 제24권4호
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• pp.287-291
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• 2012

Hemiculter eigenmanni (Jordan and Metz, 1913)의 유효성을 알아보기 위하여, Hemiculter leucisculus (Basilewsky, 1855)의 총모식 표본 2개체, H. eigenmanni (Jordan and Metz, 1913)의 완모식 표본에 대해 형태학적 분석을 수행하였다. 연구 결과, 이들 2종의 일부 형태학적 차이점들은 한국에서 채집된 다수의 비교 표본들을 통하여 종내의 변이임이 밝혀졌고, H. eigenmanni는 H. leucisculus의 신참 동종이명으로 확인되었다.

• 한국어류학회지
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• 제24권4호
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• pp.297-301
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• 2012

2010년 10월, 제주도 남부연안에서 Moolgarda seheli 3개체가 처음 채집되었다. 본 종은 비늘 가장자리에 부드러운 막이 있고, 가슴지느러미 기저의 등쪽 기점에 뚜렷한 어두운 반점과 초승달 모양의 꼬리지느러미를 가지며, 측선비늘은 39개, 척추골은 24~25개를 가진다. 본 종의 국명으로 속명은 "초승꼬리숭어속"을 새롭게 제안하며, 종명은 "초승꼬리숭어"이다.

• Journal of Species Research
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• 제12권4호
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• pp.362-367
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• 2023

Three cyclostomatous bryozoans from Korea belonging to the genera Bicrisia and Nevianipora are described here for the first time. The specimens collected from six localities of the South Sea and Jejudo Island during the period from 1981 to 2020 were observed. The three cyclostomatous bryozoans are Nevianipora pulcherrimoidea (Liu in Liu, Yin & Ma, 2001), N. rugatata (Liu in Liu, Yin & Ma, 2001), and Bicrisia edwardsiana (d'Orbigny, 1841). Nevianipora pulcherrimoidea and N. rugatata were previously found only from the South China Sea, whereas Bicrisia edwardsiana is widespread. As a result of this study, the Korean cyclostomatous bryozoans have increased to 20 species, 10 genera, and five families. Redescriptions and illustrations by scanning electron microscopy of the three species new to the Korean fauna are provided herein.

• Animal Systematics, Evolution and Diversity
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• 제40권1호
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• pp.42-46
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• 2024

Two species from the genus Escharoides Milne Edwards, 1836, E. biseriatata and E. buffonellarioides n. sp. Were first found in Korean waters in this study. Their colonies were collected from two locations in the South Sea, Korea: Tongyeong Marine Ranch in 2004 and Daebyeon Port in 2009. Escharoides biseriatata shows a difference in the position of avicularia from the Chinese specimens. While avicularia are lateral to the orifice in Chinese specimens, the ones in Korean specimens seem to be located more latero-proximally. Since Escharoides biseriatata was reported as fouling bryozoans in China in 2001, this species first occurred at Daebyeon Port in Korea in this study. Escharoides buffonellarioides n. sp. is similar to E. adeonelloides in morphological features of zooids but is clearly distinguished by having the erect colonial form of small masses. With the addition of two species reported herein, four Korean Escharoides are recorded: E. biseriatata, E. buffonellarioides, E. excavata, and E. sauroglossa.

• 한국발생생물학회지:발생과생식
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• 제27권4호
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• pp.205-211
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• 2023

INTS14/VWA9, a component of the integrator complex subunits, plays a pivotal role in regulating the fate of numerous nascent RNAs transcribed by RNA polymerase II, particularly in the biogenesis of small nuclear RNAs and enhancer RNAs. Despite its significance, a comprehensive mutation model for developmental research has been lacking. To address this gap, we aimed to investigate the expression patterns of INTS14 during zebrafish embryonic development. We generated ints14 mutant strains using the CRISPR/Cas9 system. We validated the gRNA activity by co-injecting Cas9 protein and a single guide RNA into fertilized zebrafish eggs, subsequently confirming the presence of a 6- or 9-bp deletion in the ints14 gene. In addition, we examined the two mutant alleles through PCR analysis, T7E1 assay, TA-cloning, and sequencing. For the first time, we used the CRISPR/Cas9 system to create a model in which some sequences of the ints14 gene were removed. This breakthrough opens new avenues for in-depth exploration of the role of ints14 in animal diseases. The mutant strains generated in this study can provide a valuable resource for further investigations into the specific consequences of ints14 gene deletion during zebrafish development. This research establishes a foundation for future studies exploring the molecular mechanisms underlying the functions of ints14, its interactions with other genes or proteins, and its broader implications for biological processes.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.118-118
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• 2022

Carex pumila Thunb. is a plant native to East Asia, Australia, and New Zealand. However, its effect on skin melanogenesis has not been investigated. In the present study, we evaluated its anti-melanogenic properties using B16F10 melanoma cells and zebrafish larvae in the presence or absence of α-melanocyte stimulating hormone (α-MSH). In this study we revealed that concentrations below 50 µg/mL did not induce any cytotoxicity in B16F10 melanoma cells and cardiotoxicity in zebrafish larvae. However, 50 µg/mL treatment significantly inhibited α-MSH-induced extracellular (from 181.24% α 0.62% to 105.15% α 0.31%) and intracellular melanin contents (from 119.8% α 1.2% to 53.4% α 1.7%) as well as intracellular tyrosinase activity (from 143.9% α 4.2% to 103.7% α 1.4%) in B16F10 melanoma cells. At 25 µg/mL and 50 µg/mL concentrations, it could significantly inhibit α-MSH induced hyperpigmentation in zebrafish larvae (from 100% α 2.3% to 60.7% α 1.3% and 47.5% α 1.9% respectively). Additionally, the extract suppressed α-MSH-induced cAMP-CREB-MITF signaling pathway and consequently inhibited tyrosinase expression in B16F10 melanoma cells. In conclusion, our results indicate that this plant extract could suppress the cAMP-CREB-MITF axis which consequently inhibits tyrosinase mediated melanogenesis.

• 생약학회지
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• 제28권4호
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• pp.239-246
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• 1997

The methanol extracts of some marine algae were tested for investigating the effects on the formation of lipid peroxide and the activities of free radical generating enzyme in vitro in bromobenzene-treated rat. The extracts of Enteromorpha compressa, Capsosiphon fulvescens, Gelidium amansii, Hizikia fusiformis, Sargassum siliquastrum and Sargassum thunbergii which decreased the formation of lipid peroxide, inhibited the activity of xanthine and aldehyde oxidases by adding of each extracts. Phloroglucinol isolated from Ecklonia stolonifera reduced bromobenzene-induced hepatic lipid peroxidation. This compound administered daily over one week before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase and glu tathione S-transferase. Epoxide hydrolase activity was decreased by bromobenzene, which was restored by pretreatment of phloroglucinol, The results suggest that the bromobenzene-induced hepatic lipid peroxidation by phloroglucinol is reduced by enhancing the activity of epoxide hydrolase.

• 농촌계획
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• 제13권3호
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• pp.61-72
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• 2007

Natural resource of the island and coastal regions is one of the most important element of ecological network system in Korea. Currently, there is trend that natural and biological resources such as fishing and tidal wetland have been used to local economic activation in island and coastal regions. According to the 5-days work in a week, island and coastal regions become important amenity resource of citizen's recreation area. Korea is peninsula surrounded by maritime. Especially, South and West Sea surrounded by many islands and tidal flat wetlands are emerging marine ecosystem and seascape in the world. Natural resource is limited, so we have to sustain those resource to current tourism. In this study, I would like to suggest that importance of cultural resource as well as natural resource for 'eco-cultural tourism' in order to keep both biological diversity and cultural diversity as amenity resource for future island economic activation in Korea.