• Animal cells and systems
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• v.14 no.2
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• pp.137-145
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• 2010

Four specimens of unknown Coilia sp. were collected for the first time from the Yellow Sea in 2008 and compared with Coilia mystus and Coilia nasus. Coilia sp. showed similar morphology to C. mystus and C. nasus, but differed in that its tail was considerably shorter. We conducted an analysis of the morphological and genetic characteristics in an effort to clarify the taxonomic position of Coilia sp. In counts and measurements, Coilia sp. were well distinguished from C. nasus by the number of scutes (42-44 in Coilia sp. vs. 40-45 in C. mystus vs. 45-55 in C. nasus), ratio of dorsal base length to head length (43.4-47.6 vs. 37.9-47.6 vs. 33.0-41.0), and eye length to head length (19.2-20.8 vs. 17.0-22.4 vs. 13.8-18.2). In caudal skeleton of Coilia sp., urostyle, hypural and epural bones were not observed; instead of them, caudal fin rays were supported by the last vertebra, neural and haemal spines' extension. The molecular phylogenetic relationship was analyzed using 414 base-pair 12S rRNA mitochondrial DNA sequences. The Kimura-2-parameter distance between Coilia sp. and C. mystus was 0.3%, but was 1.3% between Coilia sp. and C. nasus. Both the neighbor-joining tree and maximum-likelihood tree showed that Coilia sp. are closely clustered with C. mystus. Therefore, our results suggest that the Coilia sp. may be a deformed fish of C. mystus.

• Development and Reproduction
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• v.13 no.1
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• pp.43-49
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• 2009

We examined the effects of the vibration stress on cortisol secretion and hematological characteristics in soft-shelled turtle, Pelodiscus sinensis. For the stressed group vibration of $45{\sim}78 dB(V)$ from electric vibrator applied for 30 min with 2-h intervals during daytime ($08:00{\sim}18:00$) up to 28 days. Using the blood samples collected from ten turtles held once a week after vibration stress, we measured hematocrit, hemoglobin, red blood cells, cortisol, glucose, lactic acid, osmolality, $Na^+,\;K^+,\;Cl^－$, aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results have showed that P. sinensis received vibration stress exhibit the 'typical' stress-induced physiological responses (cortisol, glucose, lactic acid, osmolality, ions, hematocrit and hemoglobin) induced by vibration stress. Our data suggested that chronic vibration stress caused substantial stress in the animal, and in particular, the persisting elevated levels of AST and ALT would be highly correlated with the adverse effects of the stress. The high hematological characteristics during entire experimental period showed that the P. sinensis could not adapt to chronic stimuli provoked by vibration stress.

• Journal of Marine Bioscience and Biotechnology
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• v.3 no.1
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• pp.7-12
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• 2008

Cameroon is a country in Western Africa with 16 million inhabitants. Located between Nigeria and Equatorial Guinea, the country is bordered by the Bight of Biafra. It has a 402 km long coastline. It covers an area of about 475,440 sq km. Cameroon obtains its fish supply from five distinct sources notably-small scale maritime Fisheries, inland fisheries, industrial fisheries, aquaculture and importation. Despite its enormous potential, Cameroon produces only 180,000 metric tons fish annually. The total artisanal annual catch is estimated to be 55,000 t of which bonga/Sardinella, white shrimp and demersal fish contribute 58%, 27% and 15% respectively. The industrial fleet expanded rapidly during the sixties and by 1973 there were 29 trawlers and 13 shrimpers which landed a total of about 17,600 t of fish and shrimp. The total catch of the industrial fisheries peaked at about 20,400 t in 1976 and since then catches have generally declined. The per capita fish consumption in Cameroon stands at about 17.9 kg per inhabitant per year. This means that the demand of fish in Cameroon stands at about 280,000 tons. There is a deficit of in fish supply for which the government always resort to massive importation of fish products to meet the local demand. The main fish product for export from Cameroon is the prawn (Panacus duorarum). The main export market of Cameroon's prawn is the European Union (EU). Between 1998 and 2003, Cameroon's export value has been on the decline, dropping from 1,836 metric tons of prawns for a value of 264 millions USD in 1998 to 51 tonnes, for an estimated value of 315,000 USD in 2003. This drastic drop in exportation is consequential to Cameroon's auto suspension from the exportation of prawns towards the EU as a result of non compliance with EU standards. Today, a good quantity of the catches is being exported illicitly through neighbouring countries.

• Journal of Fisheries and Marine Sciences Education
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• v.12 no.1
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• pp.11-21
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• 2000

Most of artificial reefs in the examined areas were normally established except for some lower parts demersed slightly, and some to function. 1) Twenty species of fish were captured by fishing gear, and 10 species were observed by scuba in the examined areas. 2) Five algal and five fisheries animal species were observed as fouling organism. 3) C.P.U.E of trap, long liner and gill netter in the examined areas was respectively 2.12, 10.23, and 4.93, where as that in contral was established as 1.41, 4.43 and 2.86. 4) Species composition by the fishing gear the trap, long liner and gill netter in the examind areas was respectively 94, 85 and 118, where as that in control was established as 58, 37 and 62. Accordingly, The fishing effects in the artificial reefs installation sector was two times higher than comparative sector.

• Journal of Marine Bioscience and Biotechnology
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• v.14 no.1
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• pp.9-24
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• 2022

Low-molecular weight peptides derived from fish collagen exhibit several bioactivities, including antioxidant, antiwrinkle, antimicrobial, antidiabetic, and antihypertension effects. These peptides are also involved in triglyceride suppression and memory improvement. This study aimed to investigate the optimal processing condition for preparing low-molecular weight peptides from flounder skin, and the properties of the hydrolysate. The optimal processing conditions for peptic hydrolysis were as follows: a ratio of pepsin to dried skin powder of 2% (w/w), pH of 2.0, and a temperature of 50℃. Peptic hydrolysate contains several low-molecular weight peptides below 300 Da. Gly-Pro-Hyp(GPHyp) peptide, a process control index, was detected only in peptic hydrolysate on matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF) spectrum. 2,2'-azinobis-(3-3-ethylbenzothiazolline-6- sulfonic acid(ABTS) radical scavenging activity of the peptic hydrolysate was comparable to that of 1 mM ascorbic acid, which was used as a positive control at pH 5.5, whereas collagenase inhibition was five times higher with the peptic hydrolysate than with 1 mM ascorbic acid at pH 7.5. However, the tyrosinase inhibition ability of the peptic hydrolysate was lower than that of arbutin, which was used as a positive control. The antibacterial effect of the peptic hydrolysate against Propionibacterium acne was not observed. These results suggest that the peptic hydrolysate derived from a flounder skin is a promising antiwrinkle agent that can be used in various food and cosmetic products to prevent wrinkles caused by ultraviolet radiations.

• ALGAE
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• v.37 no.4
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• pp.331-347
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• 2022

Atopic dermatitis (AD) is one of major skin inflammatory diseases characterized by excessive Th2-mediated immune responses. Recent evidence provides that interlukin-13 (IL-13) plays the role of a key Th2 cytokine that drives the inflammation underlining AD. Due to adverse effects of commercially available synthetic drugs, the need for treatments based on natural products is gaining much attention. Sargassum horneri is an edible brown algae known for beneficial bioactivities including anti-inflammation. We investigated if polyphenol-rich S. horneri extracts (SHE) could suppress AD-like skin lesions in NC/Nga mice and if that involved inhibition of the infiltration of Th2-mediated cytokine IL-13. We observed markedly increased infiltration of IL-13 positive cells in AD-like skin lesions of mice but SHE treatments decreased it. Also, the dermal expression of IL-13 was sufficient to cause inflammatory responses in mice skin resembling human AD. SHE suppressed the dermal infiltration of inflammatory cells where IL-13 plays a crucial role in skin tissues and in the recruitment of inflammatory cells. Furthermore, it was confirmed that SHE reduced T cell, dendritic cell, and macrophage populations in spleen. Moreover, SHE decreased the collagen deposition in skin and ear dermis resulting in reduced fibrosis that occurs in AD due to excessive collagen. Taken together, our results reveal that SHE suppressed the infiltration of inflammatory cells into skin dermis by decreasing the infiltration of IL-13 positive cells. Therefore, SHE could be taken as a useful therapeutic agent to alleviate AD.

• The Journal of Fisheries Business Administration
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• v.54 no.3
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• pp.69-92
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• 2023

The 'Japanese Useful Fisheries Classification Table', published in advance before the publication of the "Useful Fisheries of Japan", is the first data to classify and introduce fisheries animals and plants in a single table. Therefore, it had received public attention immediately. However, The academy of animal studies at that time quickly pointed out that this classification table was a mixture of traditional and modern classifications, and that there were too many errors. However, Yoshio Tanaka, who was in charge of revising Yamamoto Arikatana, that wrote the classification table, was not an adherent of traditional taxonomy, nor was he ignorant of modern taxonomy. Nevertheless, the classification table, which was quite different from the well-known zoological classification at that time, was prepared. For example, the top classifications of marine organism are not at the same level, but rather a mixture of phylum and class, while the water insect contains several phylums, including Arthropoda, Echinodermata, Mollusca, and Coelenterata. As such, the method of classification of animals in the classification table was hard to understand in the zoological academy at that time. The reason for this unusual taxonomy was that the classification table showed classification of useful fisheries products, not intended to convey academic classification. In other words, it is not for the purpose of academic classification of all fishery products, but for the purpose of presenting standards that can be easily understood by those engaged in the collection, manufacture, and aquaculture of fishery products. This principle of 'Useful Fisheries of Japan' is also ascertained in the "Fishing Methods of Japan" and "Fishery Products of Japan". Regarding the collection and processing of marine products, it could have shown how to catch whales, which are mammals, and how to dismantle whales and obtain oil and meat, according to academic classifications. However, the first appearances in the book include dried squid, abalone, shark fins, and sea cucumbers. In other words, the most important fishery products at that time are presented first. The contents of the classification table, which is considered somewhat bizarre, show where the purpose to compile Useful Fisheries of Japan.

• Journal of fish pathology
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• v.37 no.1
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• pp.147-153
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• 2024

The World Organization for Animal Health (WOAH) recommends two protocols (ITS and COI) for conventional PCR of G. salaris diagnosis. However, ITS PCR protocol may yield false-positive results, leading to unnecessary countermeasures. It's difficult to distinguish between G. salaris and false-positive by similar amplicon size of PCR, since the amplicon size of ITS PCR in G. salaris and false-positive was 1,300 and 1,187 bp, respectively. The nucleotide sequences of ITS false-positive in rainbow trout is 99.7% identical to previously reported host genome sequences of rainbow trout (Oncorhynchus mykiss) and 95.3 to 89.1% identical to those of other salmonid fish species. To reduce false-positive PCR band, PCR was performed by the different annealing temperature, but PCR bands were still detected. In RFLP analysis by HaeIII, the PCR product of G. salaris was digested into four bands of 512, 399, 234 and 154 bp, while the false-positive was digested into seven bands of 297, 263, 242, 144, 93, 80 and 68 bp. In the RFLP patterns digested by HindIII, G. salaris showed two bands of 659 and 640 bp, while false-positive had one fragment of 1,187 bp without any digestion. Therefore, the RFLP method of ITS PCR with HaeIII and HindIII can be used for differentiation between G. salaris and false-positive. These results might provide important information on the improvement of PCR diagnostic method of G. salaris.

• Journal of Marine Life Science
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• v.9 no.1
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• pp.9-21
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• 2024

Paralytic shellfish poisoning (PSP) including Saxitoxin (STX) is caused by harmful algae, and poisoning occurs when the contaminated seafood is consumed. The mouse bioassay (MBA), a standard test method for detecting PSP, is being sanctioned in many countries due to its low detection limit and the animal concerns. An alternative to the MBA is the Neuro-2a cell-based assay. This study aimed to establish various test conditions for Neuro-2a assay, including cell density, culture conditions, and STX treatment conditions, to suit the domestic laboratory environment. As a result, the initial cell density was set to 40,000 cells/well and the incubation time to 24 hours. Additionally, the concentration of Ouabain and Veratridine (O/V) was set to 500/50 μM, at which most cells died. In this study, we identified eight concentrations of STX, ranging from 368 to 47,056 fg/μl, which produced an S-shaped dose-response curve when treated with O/V. Through inter-laboratory variability comparison of the Neuro-2a assay, we established five Quality Control Criteria to verify the appropriateness of the experiments and six Data Criteria (Top and Bottom OD, EC₅₀, EC₂₀, Hill slop, and R² of graph) to determine the reliability of the experimental data. The Neuro-2a assay conducted under the established conditions showed an EC₅₀ value of approximately 1,800~3,500 fg/μl. The intra- & inter-lab variability comparison results showed that the coefficients of variation (CVs) for the Quality Control and Data values ranged from 1.98% to 29.15%, confirming the reproducibility of the experiments. This study presented Quality Control Criteria and Data Criteria to assess the appropriateness of the experiments and confirmed the excellent repeatability and reproducibility of the Neuro-2a assay. To apply the Neuro-2a assay as an alternative method for detecting PSP in domestic seafood, it is essential to establish a toxin extraction method from seafood and toxin quantification methods, and perform correlation analysis with MBA and instrumental analysis methods.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.9
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• pp.1341-1350
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• 2009

Dietary lipids are rapidly hydrolysed and biohydrogenated in the rumen resulting in meat and milk characterised by a high content of saturated fatty acids and low polyunsaturated fatty acids (PUFA), which contributes to increases in the risk of diseases including cardiovascular disease and cancer. There has been considerable interest in altering the fatty acid composition of ruminant products with the overall aim of improving the long-term health of consumers. Metabolism of dietary lipids in the rumen (lipolysis and biohydrogenation) is a major critical control point in determining the fatty acid composition of ruminant lipids. Our understanding of the pathways involved and metabolically important intermediates has advanced considerably in recent years. Advances in molecular microbial technology based on 16S rRNA genes have helped to further advance our knowledge of the key organisms responsible for ruminal lipid transformation. Attention has focused on ruminal biohydrogenation of lipids in forages, plant oils and oilseeds, fish oil, marine algae and fat supplements as important dietary strategies which impact on fatty acid composition of ruminant lipids. Forages, such as grass and legumes, are rich in omega-3 PUFA and are a useful natural strategy in improving nutritional value of ruminant products. Specifically this review targets two key areas in relation to forages: i) what is the fate of the lipid-rich plant chloroplast in the rumen and ii) the role of the enzyme polyphenol oxidase in red clover as a natural plant-based protection mechanism of dietary lipids in the rumen. The review also addresses major pathways and micro-organisms involved in lipolysis and biohydrogenation.