• 제목/요약/키워드: Marine animal

검색결과 733건 처리시간 0.022초

The Optimum Feeding Frequency in Growing Korean Rockfish (Sebastes schlegeli) Rearing at the Temperature of 15℃ and 19℃

  • Mizanur, Rahman Md.;Bai, Sungchul C.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권9호
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    • pp.1319-1327
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    • 2014
  • Two feeding trials were conducted to determine the optimum feeding frequency in growing Korean rockfish, (Sebastes schlegeli) reared at the temperatures of $15^{\circ}C$ and $19^{\circ}C$. Fish averaging $92.2{\pm}0.7$ g (mean${\pm}$standard deviation [SD]) at $15.0{\pm}0.5^{\circ}C$ and $100.2{\pm}0.4g$ ($mean{\pm}SD$) at $19.0{\pm}0.5^{\circ}C$ water temperature were randomly distributed into each of 15 indoor tanks containing 250-L sea water from a semi-recirculation system. A total of five feeding frequency groups were set up in three replicates as follows: one meal in a day at 08:00 hour, two meals a day at 08:00 and 17:00 hours, three meals a day at 08:00, 14:00, and 20:00 hours, four meals a day at 08:00, 12:00, 16:00, and 20:00 hours, and one meal every 2 days at 08:00 hour. Fish were fed at the rate of 1.2% body weight (BW)/d at $15^{\circ}C$ and 1.5% BW/d at $19^{\circ}C$. At the end of 8 wks of feeding trial weight gain and specific growth rate were significantly higher at the fish fed groups of one meal a day and two meals a day at $15^{\circ}C$ and fish fed groups of 1 meal every 2 days at $19^{\circ}C$ were significantly lower than those of all other fish fed groups. Glutamic oxaloacetic transaminase and glutamic pyruvic transaminase of fish fed group at 1 meal every 2 days was significantly higher than those of all other fish fed groups in both experiments. Weight gain, specific growth rate and condition factor were gradually decreased as the feeding frequency increased. The results indicate that growing Korean rockfish 92 and 100 g perform better at $15^{\circ}C$ than $19^{\circ}C$ water temperature. As we expected, current results have indicated that a feeding frequency of 1 meal a day is optimal for the improvement of weight gain in growing Korean rockfish grown from 92 g to 133 g at $15^{\circ}C$ and 100 g to 132 g at $19^{\circ}C$ water temperature.

Molecular and Biochemical Characteristics of ${\beta}$-Propeller Phytase from Marine Pseudomonas sp. BS10-3 and Its Potential Application for Animal Feed Additives

  • Nam, Seung-Jeung;Kim, Young-Ok;Ko, Tea-Kyung;Kang, Jin-Ku;Chun, Kwang-Hoon;Auh, Joong-Hyuck;Lee, Chul-Soon;Lee, In-Kyu;Park, Sunghoon;Oh, Byung-Chul
    • Journal of Microbiology and Biotechnology
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    • 제24권10호
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    • pp.1413-1420
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    • 2014
  • Phytate is an antinutritional factor that impacts the bioavailability of essential minerals such as $Ca^{2+}$, $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ by forming insoluble mineral-phytate salts. These insoluble mineral-phytate salts are hydrolyzed rarely by monogastric animals, because they lack the hydrolyzing phytases and thus excrete the majority of them. The ${\beta}$-propeller phytases (BPPs) hydrolyze these insoluble mineral-phytate salts efficiently. In this study, we cloned a novel BPP gene from a marine Pseudomonas sp. This Pseudomonas BPP gene (PsBPP) had low sequence identity with other known phytases and contained an extra internal repeat domain (residues 24-279) and a typical BPP domain (residues 280-634) at the C-terminus. Structure-based sequence alignment suggested that the N-terminal repeat domain did not possess the active-site residues, whereas the C-terminal BPP domain contained multiple calcium-binding sites, which provide a favorable electrostatic environment for substrate binding and catalytic activity. Thus, we overexpressed the BPP domain from Pseudomonas sp. to potentially hydrolyze insoluble mineral-phytate salts. Purified recombinant PsBPP required $Ca^{2+}$ or $Fe^{2+}$ for phytase activity, indicating that PsBPP hydrolyzes insoluble $Fe^{2+}$-phytate or $Ca^{2+}$-phytate salts. The optimal temperature and pH for the hydrolysis of $Ca^{2+}$-phytate by PsBPP were $50^{\circ}C$ and 6.0, respectively. Biochemical and kinetic studies clearly showed that PsBPP efficiently hydrolyzed $Ca^{2+}$-phytate salts and yielded myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products. Finally, we showed that PsBPP was highly effective for hydrolyzing rice bran with high phytate content. Taken together, our results suggest that PsBPP has great potential in the animal feed industry for reducing phytates.

알코올처리에 의한 찰가자미류껍질 젤라틴의 기능성 개선 (Improvement on the Functional Properties of the Dover Sole Skin Gelatin by Further Ethanol Fractional Precipitation)

  • 조순영;하진환;이응호;김진수
    • Applied Biological Chemistry
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    • 제38권2호
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    • pp.129-134
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    • 1995
  • 수산가공부산물인 어류껍질로부터 식용으로 이용 가능한 고품질의 젤라틴을 제조할 목적으로 알코올처리에 의한 찰가자미류껍질 젤라틴의 제조를 시도하였고, 아울러 제조한 젤라틴을 목적에 맞게 효율적으로 식품산업에 이용하기 위해 필요한 몇가지 기능 특성도 검토하였다. 알코올처리 조건은 추출 및 탈색 처리한 찰가자미류껍질 젤라틴용액에 최종농도가 30%가 되도록 에탄올을 가하고 $0^{\circ}C$에서 12시간동안 분별침전한 젤라틴의 품질이 가장 우수하였고, 이 때의 겔강도, 졸화온도, 겔화온도 및 수율은 각각 223.0 g, $17.7^{\circ}C$, $12.0^{\circ}C$ 및 5.1%로서 에탄올 무처리한 젤라틴에 비해 물리적 특성이 개선되었으나 가축껍질에서 추출한 시판 젤라틴에 비하여 물리적 특성이 낮아 겔화제 등으로 이용하기에는 미흡하였다. 그러나 기능성의 경우 에탄올 처리한 어류껍질 젤라틴이 에탄올 무처리한 어류껍질 젤라틴에 비하여 용해도는 약간 낮았지만 보수력, 지방흡수력, 유화성 및 유화안정성, 거품성 및 거품안정성 등은 훨씬 높았고, 뿐만 아니라 시판되는 가축껍질 젤라틴에 필적하는 수준이었다. 따라서 겔화성 이외에의 기능성을 고려한 식품가공소재로서 에탄올로 처리한 찰가자미껍질 젤라틴을 사용하면 제품의 기능 특성은 다소 향상되리라 판단되었다.

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세균의 의사 소통(Quorum-Sensing) 기구와 그 잠재적 응용성 (Quorum-Sensing Mechanisms in Bacterial Communities and Their Potential Applications)

  • 윤성식
    • 한국축산식품학회지
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    • 제26권3호
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    • pp.402-409
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    • 2006
  • Although microorganisms are, in fact, the most diverse and abundant type of organism on Earth, the ecological functions of microbial populations remains poorly understood. A variety of bacteria including marine Vibrios encounter numerous ecological challenges, such as UV light, predation, competition, and seasonal variations in seawater including pH, salinity, nutrient levels, temperature and so forth. In order to survive and proliferate under variable conditions, they have to develop elaborate means of communication to meet the challenges to which they are exposed. In bacteria, a range of biological functions have recently been found to be regulated by a population density-dependent cell-cell signaling mechanism known as quorum-sensing (QS). In other words, bacterial cells sense population density by monitoring the presence of self-produced extracellular autoinducers (AI). N-acylhomoserine lactone (AHL)-dependent quorum-sensing was first discovered in two luminescent marine bacteria, Vibrio fischeri and Vibrio harveyi. The LuxI/R system of V. fischeriis the paradigm of Gram-negative quorum-sensing systems. At high population density, the accumulated signalstrigger the expression of target genes and thereby initiate a new set of biological activities. Several QS systems have been identified so far. Among them, an AHL-dependent QS system has been found to control biofilm formation in several bacterial species, including Pseudomonas aeruginosa, Aeromonas hydrophila, Burkholderia cepacia, and Serratia liquefaciens. Bacterial biofilm is a structured community of bacterial cells enclosed in a self-produced polymeric matrix that adheres to an inert or living surface. Extracellular signal molecules have been implicated in biofilm formation. Agrobacterium tumefaciens strain NT1(traR, tra::lacZ749) and Chromobacterium violaceum strain CV026 are used as biosensors to detect AHL signals. Quorum sensing in lactic acid bacteria involves peptides that are directly sensed by membrane-located histidine kinases, after which the signal is transmitted to an intracellular regulator. In the nisin autoregulation process in Lactococcus lactis, the NisK protein acts as the sensor for nisin, and NisR protein as the response regulator activatingthe transcription of target genes. For control over growth and survival in bacterial communities, various strategies need to be developed by which receptors of the signal molecules are interfered with or the synthesis and release of the molecules is controlled. However, much is still unknown about the metabolic processes involved in such signal transduction and whether or not various foods and food ingredients may affect communication between spoilage or pathogenic bacteria. In five to ten years, we will be able to discover new signal molecules, some of which may have applications in food preservation to inhibit the growth of pathogens on foods.

명태, 폐계가슴살 및 기계발골계육을 활용한 수리미의 품질 특성 (Quality Characteristics of Surimi Manufactured by Alaska Pollack, Barren Hen Breast Meat and Mechanically Deboned Chicken Meat)

  • 진상근;김일석;양한술;최영준;김병균[한성식품
    • Journal of Animal Science and Technology
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    • 제49권3호
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    • pp.395-404
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    • 2007
  • 수리미 원료육의 특성을 분석하기 위하여 폐계가슴살(T1), 기계발골계육인 S사 MDCM(T2) 및 J사 MDCM(T3)을 활용하여 pH 11.0의 알칼리 조절법으로 수리미를 제조한 후 품질 특성을 비교한 결과 명태연육을 활용한 수리미인 대조구는 다른 처리구들에 비해 백색도, 응집성, 관능평가의 기호도가 높은 장점을 나타낸 반면 검성, 씹힘성 및 겔 특성이 낮은 단점을 나타내었다. T1은 다른 처리구들에 비해 명도, 표면경도, 변형값, 젤리강도 및 관능평가의 향이 높은 장점을 나타낸 반면 조직감 및 접기실험 결과가 낮게 나타났다. T2는 다른 처리구들에 비해 조직감, 전단가 및 겔 특성이 높은 반면 변형값, 관능평가의 기호도가 낮고, 황색도가 높은 단점을 나타내었다. 또한 T3의 경우 다른 처리구들에 비해 응집성이 가장 높았으며, 적색도가 높고, 명도 및 백색도 낮은 단점을 나타내었다. 결론적으로 원료육의 차이에 따른 수리미의 품질 특성을 비교한 결과 폐계가슴살을 활용한 T1에서 높은 단백질 함량과 낮은 지방 함량을 보여 수리미 원료로서의 활용도가 높을 것으로 판단된다. 또한 수리미 주요 겔 특성인 변형값과 응집성을 중심으로 볼 때 명태연육을 대체할 수 있는 수리미 원료육으로 T1과 T3가 뛰어난 것으로 나타났다. 그러나 T1과는 달리 MDCM(T2 및 T3)은 적색도가 높고 백색도가 낮아 수리미 제조시 외관적 제품 특성에 부정적인 영향을 미칠 뿐만 아니라 기호도에서 낮은 점수를 획득하여 이에 대한 추가 연구가 필요할 것이라 판단된다.

국내 포유류 연구의 주제와 동향 (Mammalian Research Topics and Trends in Korea)

  • 고병준;어수형
    • 한국환경생태학회지
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    • 제31권1호
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    • pp.30-41
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    • 2017
  • 국내에서 포유류는 축산학, 수의학, 실험동물학, 생태학, 유전학 등 다양한 분야에서 활발하게 연구되고 있으며, 최근 생물다양성의 중요성이 강조되면서 이들의 보전 및 관리는 국민적으로도 많은 관심을 받고 있다. 하지만 전문 연구와 국민 관심의 증가에도 불구하고 국내 포유류 연구동향을 파악한 사례는 아직까지 찾아보기 힘들다. 이 연구는 국내 포유류의 연구동향을 파악하여 향후 세부연구영역의 계획과 관련 정책 제시를 위한 기초자료 제공을 목표로 하였다. 2015년까지 국내에서 발행된 포유류 학술논문 392편을 분석 대상으로 하였으며, 최근 각광받는 연구영역을 파악하기 위해 텍스트마이닝과 동시출현단어 분석을 이용하였다. 그 결과, 국내 포유류 연구논문 발행 수는 점차 증가하였으며, 연구대상 종 역시 점차 다양해진 것으로 나타났다. 텍스트마이닝과 동시출현단어 분석을 통해 파악된 주된 포유류 연구영역은 (1)진화/계통/유전학, (2)환경/생태학, (3)발생/생식/세포생물학, (4)기생충/수의학, (5)설치류/기생충학, (6)세균/바이러스학, (7)해부/세포생물/실험동물학, (8)형태/해부수의학, (9)축산학, (10)해양포유류학, (11)익수목 연구 등 11개로 구분되었다. 환경/생태학 연구는 11개 연구영역 중에서 최근 가장 활발하였으며, 과거에 비해 연구비율이 급격히 증가한 분야로 나타났다. 환경/생태학 연구분야는 생물다양성 보전의 핵심으로, 최근 생물다양성의 중요성이 강조됨에 따라 국내 서식 포유류의 생태연구에 대한 연구자들의 관심 역시 더욱 증가한 것으로 보인다. 이 연구결과가 미래 국내 포유류 연구의 계획과 관련 정책 수립을 위한 기초자료로 유용하게 활용되기를 희망한다.

조피볼락의 사료원으로 갈색거저리의 영양적 가치 및 소화율 평가 (Nutritional Value and Digestibility of Tenebrio molitor As a Feed Ingredient for Rockfish (Sebastes schlegeli))

  • 장태호;정성목;김에스더;이용석;이상민
    • 수산해양교육연구
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    • 제29권3호
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    • pp.888-898
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    • 2017
  • Tenebrio molitor larvae, also known as yellow mealworms (MW), are rich in protein and lipid and can serve as a potential alternative protein and energy source in commercial aquafeeds. Therefore, this study attempts to evaluate the effects of different drying methods on the nutritional value of MW meal. For this, live MW were cold-anaesthetized before being subjected to three different types of drying methods, including freeze-drying, oven-drying at $60^{\circ}C$ and air-drying at room temperature for three days, and compared for proximate composition and energy content. An in-vivo digestibility test was then conducted to evaluate the nutrient digestibility of MW meal in diets for rockfish, Sebastes schlegeli. A test diet was prepared by mixing the MW meal with a reference diet (Ref) in a 30:70 ratio with chromium oxide as an inert marker at the inclusion level of 0.5%. Rockfish with mean body weight of 150 g were stocked into a fecal collection system equipped with fiberglass tanks of 400 L capacity. Each group of fish was fed one of the experimental diets to apparent satiation for 4 weeks. The results of the proximate analysis showed that drying methods had no significant effect on crude protein, crude lipid, ash and energy contents of MW. Despite being a rich source of protein and lipid, MW meal was deficient in certain amino acids, particularly methionine, and highly unsaturated fatty acids, particularly 22:6n-3 (DHA) and 20:5n-3 (EPA). MW meal showed high digestibility values for protein (93%), lipid (97%) and energy (88%). These results may indicate that MW meal is a nutritious and acceptable feed ingredient, with comparable digestibility values to conventional animal and plant feedstuffs such as fish meal and soybean meal, in practical diet for rockfish at grower stage.

Production of Oleamide, a Functional Lipid, by Streptomyces sp. KK90378

  • Kwon, Jeong-Ho;Hwang, Sung-Eun;Han, Jae-Taek;Kim, Chang-Jin;Rho, Jung-Rae;Shin, Jong-Eon
    • Journal of Microbiology and Biotechnology
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    • 제11권6호
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    • pp.1018-1023
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    • 2001
  • Oleamide (cis-9-octadecenamide) is endogenous primary amide of fatty acid that is produced in small amounts in animal brains. It is known to induce sleep and to lower temperature by destroying the lipid plasma membrane structure of cells, thereby disclosing gap junction channels. To develop a new biological production method for oleamide, a screening program was conducted to isolate a microorganism producing oleamide. Among 1,500 soil microorganisms tested, KK90378 exhibited a potent positive reaction with Dragendoff`s reagent, used to detect the primary amide of oleamide. KK90378 was identified as a Streptomyces species based on cultural and morpohological characteristics, the presence of diaminopimelic acid in the cell wall, and the sugar patterns for the whole-cell extrat. Streptomyces sp. KK90378 produced oleamide 3 days after culture at $28^{\circ}C$, pH 7.2 A series of purification steps, including hexane extraction, silica gel column, and preparative thin layer chromatographies, were performed for the purification of oleamide. A spectrophotometric analysis using $^1H$, $^13C$-NMR, and GC-MS confirmed that the chemical structure of the purified oleamide was identical to that of authentic oleamide.

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해수산 클로렐라(Chlorella ellipsoidea) 유기용매 추출물의 항염증 효과 (Anti-Inflammatory Effect of Chlorella ellipsoidea Extracted from Seawater by Organic Solvents)

  • 최유진;조월순;김현지;남병혁;강은영;오수정;이계안;정민호
    • 한국수산과학회지
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    • 제43권1호
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    • pp.39-45
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    • 2010
  • Chlorella has been reported to have certain beneficial physiological effects, including hypocholesterolemic, antihypertensive, antioxidative, and anti-tumor activities in animal and human studies. The aim of this study was to determine the anti-inflammatory activities of an 80% methanol extract(CE-Met), hexane fraction (CE-Hex), and ethyl acetate fraction (CE-EA) of Chlorella ellipsoidea in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage. Treatment with various concentrations of the C. ellipsoidea extract resulted m a significant, dose-dependent reduction in nitric oxide (NO) production by LPS-induced macrophages. The C. ellipsoidea extract significantly inhibited LPS-induced NO production accompanied by an attenuation of IL-6 and TNF-$\alpha$ formation in macrophages. These results suggest potent inhibit이y effects on the production of inflammatory mediators by a C. ellipsoidea extract. Thus, C. ellipsoidea extract may be a potent anti-inflammatory agent for troubled skin.

해양소재를 이용한 마스크팩의 유용성 : 피부재생효과 (Usefullness of Mask Pack Sheets Including Marine Materials: Skin Regeneration Effect)

  • 박대환;박상욱;최성곤
    • 한국수산과학회지
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    • 제45권2호
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    • pp.167-172
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    • 2012
  • Minerals from marine materials such as deep ocean water and Dead Sea water have been used since ancient times. We made a mask pack sheet including deep ocean water and salt from the Dead Sea and evaluated the function of the mask pack sheet through animal study. Three full-thickness skin defects were made on the backs of Sprague-Dawley rats. The wounds were left untreated in group Con, and mask pack sheets including deep ocean water or deep ocean water and Dead Sea water were used as treatment for 20 min on the skin of animals in groups DP and DDP, respectively. We analyzed the gross, histological and biochemical findings. Groups DDP and DP showed decreases in wound size, as compared to group Con at 7 days after wound infliction. The histological findings revealed that wound healing had progressed further in groups DP and DDP than in group Con, with more rapid collagen deposition and regression of neutrophils. Also, the expression of vascular endothelial growth factor and transforming growth factor ${\beta}1$ were increased in groups DDP and DP compared with those in group Con at 3 days after wound infliction. Mask sheet packs including deep ocean water and Dead Sea salt affected wound healing by reducing the inflammatory phase and stimulated wound contracture by facilitating the deposition of collagen.