• 한국수산과학회지
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• 제46권4호
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• pp.351-358
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• 2013

A protease inhibitor from fish eggs was fractionated using chromatographic methods. The fractionation efficiency was evaluated in terms of specific inhibitory activity (SIA, U/mg), purity (fold), total inhibitory activity (TIA, U), and recovery (%). The protease inhibitor (PI) from egg extracts of skipjack tuna (ST Katsuwonus pelamis), yellowfin tuna (YT Thunnus albacares) and Alaska pollock (AP Theragra chalcogramma) was fractionated using Sephadex G-50 gel filtration and DEAE-Sepharose CL-6B anion exchange chromatography based on protein size exclusion and net charge, respectively. Fractions exhibiting strong inhibitory activity were contained in the 30-50 kDa fraction on gel filtration and in the range of 0.4-0.7 M NaCl gradient fraction on anion exchange chromatography. The respective TIA and percent recovery of the fraction obtained with gel filtration toward trypsin and $N{\alpha}$-benzoyl-L-arginine-p-nitroanilide (BAPNA) were 2,758.7 U and 29.6% for ST, 1,005.5 U and 25.6% for YT, and 1,267.5 U and 26.0% for AP. Gel filtration chromatography was more effective at fractionating PI than using ion exchange chromatography. These results suggest that fish eggs act as serine protease inhibitors and might be useful for protease inhibition in foodstuffs.

• 한국수산과학회지
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• 제47권2호
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• pp.135-143
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• 2014

Exopeptidase active fractions from the hepatopancreas of the Argentina shortfin squid Illex argentinus, were obtained with acetone (AC 30-40%), ammonium sulfate (AS 60-70% saturation), anion exchange chromatography (AE-II, 0.2 M NaCl) and gel filtration chromatography (GF-I, 30-50 kDa) fractionation methods. A bitter peptide solution that has a bitterness equivalent to that of 2% glycylphenylalanine and prepared by tryptic hydrolysis of milk casein, was treated with the exopeptidase active fractions. The GF-I fraction was the best based on aminopeptidase activity (35.3 U/mg), percentage of recovery (30.7%) and a sensory evaluation (1.7). The amount of released amino acids increased as incubation time increased, and the bitterness of the enzyme reaction mixtures decreased. Incubation with the GF-I fraction for 24 h resulted in the hydrolysis of several peptides as revealed by the reverse-phase high performance liguid chromatography profile, with three peaks (3, 5 and 6) decreasing in area (%) and three peaks (1, 2 and 4) increasing in area (%). Therefore, the GF-I fraction appeared to be ideally suited to reduce bitterness in protein hydrolysates by catalyzing the hydrolysis of bitter peptides.

• 한국식품위생안전성학회지
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• 제36권6호
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• pp.455-473
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• 2021

이 총설에서는 해양 연체동물 내 독소 분석을 하는 연구자들의 이해를 돕기 위하여, 국내에서 생산되거나 유통되는 수산물 중 해양 생물독소에 오염될 수 있는 이매패류와 복족류 종들의 공식 명칭과 생태학적 특성을 소개하였다. 또한 대표적인 생물군의 해부도를 통한 각 기관들의 위치와 공식적인 명칭을 알리고자 하였다. 향후 해양생물독소 분석 관련 식품공전, 학술논문 및 보고서에 실험생물의 정확한 종명과 분석 기관의 명칭에 대한 통일 및 규격화가 필요하다.

• Corrosion Science and Technology
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• 제8권6호
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• pp.243-250
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• 2009

When a steel sheet pipe applied to marine environment, an anti-corrosive coating should be treated to obtain long-term life-time for steels, especially, splash zone. Although anti-corrosive property of coatings is required to be tested in real marine environment, it is difficult because of long test time such as 20 years or more time. Therefore, we used cyclic corrosion tester in a laboratory, which has similar conditions with salt-dry-wet process such as real marine environment. Anti-corrosive properties of the coatings and two steels were tested their anti-corrosive properties under cyclic corrosion test conditions(KS D ISO 14993) and the results were compared with estimate life-time in real marine environment. According to cyclic corrosion test, accelerated corrosive factor of each anti-corrosive coating was investigated accelerated corrosive factor from impedance with EIS method. Accelerated corrosive factor of type SS400 carbon steel and A690 was also investigated their accelerated corrosive factor from the regression curves of weigh loss results. One of the anti-corrosive coatings showed about 50 years life-time compared with standard sample life-time. Carbon steel SS400 showed from 0.1 mm/yr to 0.06 mm/yr as its corrosion rate.

• 한국해양환경ㆍ에너지학회지
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• 제17권4호
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• pp.274-282
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• 2014

정부는 해양쓰레기에 대한 모니터링 및 환경영향평가 등 과학적인 연구사업을 통해 해양쓰레기의 영향을 저감시키고 체계적으로 관리하기 위한 해양쓰레기 오염대응 기술개발사업의 시행을 고려하고 있다. 이에 본 연구에서는 이 사업에 대한 경제적 타당성을 분석함으로써 정책판단에 있어서 유용한 정보를 제공하고자 한다. 이를 위해 경제성 분석 지표인 순현재가치, 편익/비용 비율, 내부수익률을 분석하여 제시한다. 분석결과 순현재가치, 편익/비용 비율, 내부수익률은 각각 457억원, 2.72, 17.12%로 산정되어 각각 0, 1.0, 5.5%를 상회하므로 해양쓰레기 오염대응 기술개발사업은 비용-편익 분석을 통과한다. 따라서 해양쓰레기 오염대응 기술개발사업을 시행하는 것은 사회적으로 바람직하다.

• 한국수산과학회지
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• 제44권1호
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• pp.31-36
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• 2011

This study developed a normal temperature-circulatable retort-sterilized crab analog (RSCA) with a long shelf-life by retort sterilization. We examined the optimum sterilization conditions and quality characteristics of the RSCA. A central composite design for response surface methodology (RSM) was adopted to optimize of the retort sterilization. The central composite design consisted of 11 samples: four factorial points, four star points, and three central points. The sterilization temperature and $F_0$-value for retort sterilization were the independent variables, and the shearing force, whiteness, and sensory score for texture were the dependent variable. The RSM analysis of the multiple response optimization for the RSCA using the Minitap statistical programing gave an $F_0$-value of 3.3 min. at $117.5^{\circ}C$. The RSM analysis also indicated that the sterilization temperature during retort sterilization was the most influential factor, while the $F_0$-value had little effect on the quality of the RSCA. The moisture, pH and volatile basic nitrogen contents of the RSCA product were 73.1%, 7.3 and 17.2 mg/100 g, respectively, and viable cells count was >18 CFU/g. And sensory quality characteristics of this RSCA was similar to those of a commercial crab analog.

• 한국군사과학기술학회지
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• 제25권3호
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• pp.231-238
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• 2022

Mine detection has been mainly studied with images of the forward-looking sonar. Forward-looking sonar assumes the propagation path of the sound wave as a straight path, creating the surrounding images. This might lead to errors in the detection by ignoring the refraction of the sound wave. In this study, we propose a mine localization method that can robustly identify the location of mines in an underwater environment by considering the refraction of sound waves. We propose a method of estimating the elevation angle of arrival of the target echo signal in a single receiver, and estimate the mine location by applying the estimated elevation angle of arrival to ray tracing. As a result of simulation, the method proposed in this paper was more effective in estimating the mine localization than the existing method that assumed the propagation path as a straight line.

• Food Science and Biotechnology
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• 제14권3호
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• pp.433-436
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• 2005

Chitosans were obtained with varying deacetylation times using the ${\beta}$-chitin isolated from Todarodes pacificus, and their deacetylation degrees and average molecular weights were determined. Films prepared with the squid chitosans were characterized by estimating their tensile strengths, percent elongations, water vapor permeabilities, degree of swelling, and temperatures of glass transition and thermal decomposition. The results suggest that the squid chitosan films were comparable to common crustacean chitosan films in regard of mechanical, moisture barrier, and thermal properties, although further, multilateral investigations are necessary to make a more definitive conclusion.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.830-833
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• 2005

The diversification of the regulating system was promoted with the development of the computer technology. On the other hand, environmental problems and the energy conservation problem are being valued as for needs of the society. Our research was able to be achieved in such a situation.

• 한국수산과학회지
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• 제44권5호
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• pp.435-442
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• 2011

This study was conducted to optimize the processing of high quality surimi gel from unmarketable cultured bastard halibut Paralichthys olivaceus. According to endogenous enzyme activity and processing optimization, high quality surimi gel from unmarketable cultured bastard halibut was prepared by mixing 3.0% (w/w) salt, 2.4% (w/w) starch, 5.0% (w/w) egg white and 4.8% (w/w) ice water in a Stephan mixer, set at $5^{\circ}C$ for 24 h, followed by boiling for 30 min, and finally cooling for 30 min. The strength of the surimi gel from unmarketable cultured bastard halibut prepared by the above processing method was $1,257\;g{\times}cm$, which was 33% higher than that of a commercial surimi gel from Alaska pollock, grade SA.