• 대한화장품학회지
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• 제36권1호
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• pp.79-87
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• 2010

자외선은 생체 내 활성산소 생성 증가와 내인성 항산화 효소 및 항산화제 감소를 통하여 광노화 과정을 촉진하는 대표적인 외부 환경인자로 알려져 있다. 그러므로 본 연구에서는 12종의 다양한 해양소재 추출물(김, 다시마, 모려, 모자반, 미역, 석결명, 청각, 해룡, 해마, 해삼, 파래, 톳)을 이용하여 기본적인 자유라디칼 소거능 실험을 바탕으로, 자외선 B 조사로 인한 세포독성 및 산화적 사멸을 억제하고 세포 내 항산화 기능을 강화시킬 수 있는 천연 항산화 피부보호소재를 발굴하고자 하였다. 해양소재 추출물의 라디칼 소거능을 1,1-diphenyl-2-picrylhydrazyl (DppH) assay로 비교 측정한 결과 미역, 모자반, 다시마, 해마, 석결명 모려, 해룡의 순으로 항산화력을 나타내었다. 이후 in vitro 세포 실험에서 자외선 B를 섬유아세포주인 HS68 세포에 노출시킨 경우 세포 독성이 유발되어 사멸이 진행되었으며 이는 모자반, 석결명, 청각, 해마 열수 추출물을 처리한 경우 현저히 억제되었다. 특히, 자외선 B로 인한 세포손상은 세포 내 활성산소종의 축적으로 인한 것임을 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) 형광염색법으로 확인할 수 있었으며, 활성산소종의 생성은 모자반, 석결명, 청각, 해마 열수 추출물을 처리한 경우 유의적으로 감소되었다. 한편, 이러한 항산화 작용은 대표적인 내인성 항산화 효소인 catalase, superoxide dismutase 및 heme oxygenase-1의 발현 증가로 매개되었다. 이상의 결과는 모자만, 석결명, 정각, 해마 풍의 해양소재가 항산화 작용을 통하여 산화적 스트레스가 매개하는 피부손상과 노화과정을 예방 및 보호하는 새로운 화장품 천연소재로의 활용 가능성을 제시한다.

• 한국해양생명과학회지
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• 제7권2호
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• pp.121-128
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• 2022

L-asparaginase (ASNase) is a therapeutic enzyme used to treat acute lymphoblastic leukemia. Currently, the most widely used ASNases are originated from bacteria. However, owing to the adverse effects of bacterial ASNases, new resources for ASNase production should be explored. Fungal enzymes are considered efficient and compatible resources of natural products for diverse applications. In particular, fungal species belonging to the genus Trichoderma are well-known producers of several commercial enzymes including cellulase, chitinase, and xylanase. However, enzyme production by marine-derived Trichoderma spp. remains to be elucidated. While screening for extracellular ASNase-producing fungi from marine environments, we found four strains showing extracellular ASNase activity. Based on the morphological and phylogenetic analyses using sequences of translation elongation factor 1-alpha (tef1α), the Trichoderma isolates were identified as T. afroharzianum, T. asperellem, T. citrinoviride, and Trichoderma sp. 1. All four strains showed different ASNase activities depending on the carbon sources. T. asperellem MABIK FU00000795 showed the highest ASNase value with lactose as a carbon source. Based on our findings, we propose that marine-derived Trichoderma spp. are potential candidates for novel ASNase production.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2004년도 Proceedings of ISRS 2004
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• pp.494-497
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• 2004

To date the KOMPSAT OSMI(Ocean Scanning Multi-spectral Imager) data have been widely used in natural disaster monitoring such as Typhoon, Asian Dust, Red Tide, and Forest Fire. Quantitative analyses related to the marine ecosystem have been delayed because they require good quality of data through Cal/Val activities. To resolve such problem, KARI performed the OSMI crosscalibration study with SeaWiFS team. In this study, we will demonstrate the OSMI ocean color products with updated cross-calibration coefficients and compare them to the previous cross-calibration results.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.304.1-304
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• 1998

No Abstract, See Full Text

• 해양환경안전학회지
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• 제8권2호
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• pp.53-60
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• 2002

Marine biotechnology is one of the promising frontier of scientific exploration and commercial utilization for the next century. Compared with the terrestrial environment, the oceans of the world remain largely unexplored and include a major portion of bio-resources. Using the tools of biotechnology, the vast and diverse marine resources can be applied to produce new products and foods. Marine biotechnology has the characteristics of pro-environment, saying energy, and intensive knowledge. Therefore, we can take advantage of the marine biotechnology industry under our situation with the poor natural resources. The study focuses on the current status and administrative supports on marine biotechnology industry for upgrading the economic value of output. The status of our marine biotechnology industry is beginning stage in the economic aspects. Manpower and the level of most technologies are weaker than the ones of the advanced countries. More investment and recruiting skilled specialists are necessary because the improvement of marine biotechnology is depend on the technology and scientists. This study suggests the ways of administrative supports for domestic marine biotechnology: Efficient information network and supporting system for the development of marine biotechnology should be interrelated with other technical and scientific fields; The government should provide sustainable fund for the long-term research project and the infrastructure in the marine biotechnology.

• Archives of Pharmacal Research
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• 제26권7호
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• pp.532-534
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• 2003

Kojic acid dimethyl ether (1), and the known kojic acid mono methyl ether (2), kojic acid (3) and phomaligol A (4) have been isolated from the organic extract of the broth of the marine-derived fungus Altenaria sp. collected from the surface of the marine green alga Ulva pertusa. The structures were assigned on the basis of comprehensive spectroscopic analyses. Each isolate was tested for its tyrosinase inhibitory activity. Kojic acid (3) was found to have significant tyrosinase inhibitory activity, but compounds 1, 2, and 4 were found to be inactive.

• 한국해양바이오학회지
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• 제2권4호
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• pp.230-233
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• 2007

Protein tyrosine phosphatase 1B (PTP1B) acts as a negative regulator of insulin signaling, and selective inhibition of PTP1B has served as a potential drug target for the treatment of type 2 diabetes. As part of our searching for PTP1B inhibitors from natural products, the extracts of marine microorganisms were screened for the inhibitory effects on the activity of protein tyrosine phosphatase 1B (PTP1B). Among the tested 304 extracts, 29 extracts exhibited inhibition rate ranging 40.1 - 83.6 % against PTP1B at the concentration level of $30{\mu}g/mL$.

• Natural Product Sciences
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• 제10권6호
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• pp.272-276
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• 2004

During the course of our screening for bioactive metabolites from marine sponges, EZZ, the inseparable 1:1 mixture of (8E,13Z,20Z)-strobilinin and (7E,13Z,20Z)-felixinin has been found to deliver significant cytotoxicity against some cancer cell lines. In this study, the antioxidant activity of EZZ was first time evaluated by a series of antioxidant assays. It was found that EZZ was weak in scavenging the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), but it was comparable to ascorbic acid in scavenging ABTS and superoxide radicals. In addition, EZZ could protect DNA from hydroxyl radical-induced strand cleavage. The findings of the present study suggest that EZZ possess certain antioxidant activity, which might help to prevent occurrence of cancer by alleviating the oxidative stress in cells.

• 수산해양교육연구
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• 제21권3호
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• pp.373-389
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• 2009

The aims of this study are to examine urgent problems for reorganization into future-proof large purse seine fisheries in order to positively cope with rapidly changing domestic and foreign fisheries circumstances. First, this paper provides current situations of the large purse seine fisheries. The current situations of it are composed of fisheries influence such as vessel number, vessel power, and fisher number, use of resource and fishing places, and changes in products and sales of species caught by the large purse seine. Secondly, this paper reviews current problems of the large purse seine fisheries including cost reduction and increase of value added. Thirdly, this paper suggests basic development direction and countermeasures for strengthening competitiveness of the large purse seine fisheries. In conclusion, urgent problems of the larger purse seine fisheries can be summarized as inefficiency of distribution structures and retarded landing system and facilities as well as the worse of profitability according to shrink of fishing places, reduction of products, lack of workers, and increase in oil price. To solve the urgent problems, the large purse seine fisheries should be changed into an industry with low cost and high efficiency, and also need to introduce of new production system, strengthen autonomous management of natural resource, and increase in value added to products.

• BMB Reports
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• 제48권5호
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• pp.289-294
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• 2015

Caesalpinia sappan is a well-distributed plant that is cultivated in Southeast Asia, Africa, and the Americas. C. sappan has been used in Asian folk medicine and its extract has been shown to have pharmacological effects. Two homoisoflavonoids, sappanol and brazilin, were isolated from C. sappan by using centrifugal partition chromatography (CPC), and tested for protective effects against retinal cell death. The isolated homoisoflavonoids produced approximately 20-fold inhibition of N-retinylidene-N-retinyl-ethanolamine (A2E) photooxidation in a dose-dependent manner. Of the 2 compounds, brazilin showed better inhibition (197.93 ± 1.59 μM of IC₅₀). Cell viability tests and PI/Hoechst 33342 double staining method indicated that compared to the negative control, sappanol significantly attenuated H₂O₂-induced retinal death. The compounds significantly blunted the up-regulation of intracellular reactive oxygen species (ROS), and sappanol inhibited lipid peroxidation in a concentration-dependent manner. Thus, both compounds represent potential antioxidant treatments for retinal diseases. [BMB Reports 2015; 48(5): 289-294]